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The map label for this gene is tuf-2 [H]

Identifier: 88607578

GI number: 88607578

Start: 1094197

End: 1095378

Strand: Reverse

Name: tuf-2 [H]

Synonym: APH_1032

Alternate gene names: 88607578

Gene position: 1095378-1094197 (Counterclockwise)

Preceding gene: 88606747

Following gene: 88606977

Centisome position: 74.45

GC content: 45.35

Gene sequence:

>1182_bases
ATGACAGAAGGAAGGAAGCCGCATATAAACGTAGGGACAATAGGGCACGTGGATCACGGTAAGACAACGCTTACAGCTGC
GTTAACTACTGTTCTGGCTAGAAAGCTAAGTGGCGCGAACAAAGTGGTGAAGTATGATGAGATAGACAAGGCGCCAGAAG
AAAAGGCAAGAGGAATAACAATATCAACAGCGCATGTGGAGTATGAGACTGAAGGTAGACACTATGCGCATGTAGACTGT
CCGGGGCACGCTGACTACATTAAGAACATGATTACTGGAGCGGCGCAGATGGATGTTGCGATACTGGTTGTGTCGGCGAC
AGACGGAGCGATGCCGCAAACTCGTGAGCATATATTGTTGGCGAAGCAGGTTGGTGTAAAAGACATCGTGGTGTGGATTA
ACAAATGCGATGTAGTTGAAGATGAAGAAATGTTATCGCTCGTGGATATGGAGATACGCGAGTTATTGAGTCAGTATGGA
TATGATGGTGATTCTATCGATGCTGTAAGAGGGTCTGCGGTTAAGGCATTGGAAGAAGATGCTGATGGTCCTTGGAGTGA
TAAGATAATGGAATTGGTAGGTGCTCTGGAAAAGATAGAGCTGCCGATGAGAGAGAAGGACAAGCCGTTTTTGATGTCTG
TAGAAGACGTGTTCTCTATACCTGGACGTGGAACAGTGGTTACAGGAAGAATAGAGAGAGGCGTAGTAAGAGTCGGAGAC
AAGATAGATATTGTAGGTCTGCGTGAATTACAAAGTACGGTTTGTACTGGAGTAGAAATGTTCCATAAGGCATTGGAAGC
TGGTGAAGCTGGTGACAATGCTGGGATACTTCTCAGAGGTATAAAGAAGGAAGACGTAGAGAGAGGTCAAGTACTCAGTG
CGCCTGGGCAAATGAAGTCATATAAGAAGTTCAAGGCGGAAGTATACGTATTGAAGAAGGAAGAGGGAGGAAGGCATACT
CCATTCTTTGCTAATTACCAACCTCAGTTCTACGTTAGAACTACGGATGTAACAGGAAGCATATCGTTACCTGCGGGTGT
AGAAATGGTTATGCCTGGAGATAATCTAAGTATAGAGGTAGCGTTGGATAAGCCTGTTGCGATAGATAAGGGGCTTAGGT
TTGCTGTGAGAGAAGGTGGAAGGACTGTTGGCTCAGGTATTATTACTGAGATTTTGGAGTAG

Upstream 100 bases:

>100_bases
CTATGTCTCAGGGTAGAGCACAGTATAGTATGTTCTTTGCTCGTTATGAAAGGGTGCCTGAGAACGTTGTTGTTAATGAT
GTGAAGAGGTAGGTATTGTT

Downstream 100 bases:

>100_bases
TTTTTATATCTGGAGTTATAAGCTAGGAGTGTAGTTCAATGGTAGAACATCAGTCTCCAAAACTGAGAGTATGGGTTCGA
CTCCTGTCACTCCTGCCATA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTEGRKPHINVGTIGHVDHGKTTLTAALTTVLARKLSGANKVVKYDEIDKAPEEKARGITISTAHVEYETEGRHYAHVDC
PGHADYIKNMITGAAQMDVAILVVSATDGAMPQTREHILLAKQVGVKDIVVWINKCDVVEDEEMLSLVDMEIRELLSQYG
YDGDSIDAVRGSAVKALEEDADGPWSDKIMELVGALEKIELPMREKDKPFLMSVEDVFSIPGRGTVVTGRIERGVVRVGD
KIDIVGLRELQSTVCTGVEMFHKALEAGEAGDNAGILLRGIKKEDVERGQVLSAPGQMKSYKKFKAEVYVLKKEEGGRHT
PFFANYQPQFYVRTTDVTGSISLPAGVEMVMPGDNLSIEVALDKPVAIDKGLRFAVREGGRTVGSGIITEILE

Sequences:

>Translated_393_residues
MTEGRKPHINVGTIGHVDHGKTTLTAALTTVLARKLSGANKVVKYDEIDKAPEEKARGITISTAHVEYETEGRHYAHVDC
PGHADYIKNMITGAAQMDVAILVVSATDGAMPQTREHILLAKQVGVKDIVVWINKCDVVEDEEMLSLVDMEIRELLSQYG
YDGDSIDAVRGSAVKALEEDADGPWSDKIMELVGALEKIELPMREKDKPFLMSVEDVFSIPGRGTVVTGRIERGVVRVGD
KIDIVGLRELQSTVCTGVEMFHKALEAGEAGDNAGILLRGIKKEDVERGQVLSAPGQMKSYKKFKAEVYVLKKEEGGRHT
PFFANYQPQFYVRTTDVTGSISLPAGVEMVMPGDNLSIEVALDKPVAIDKGLRFAVREGGRTVGSGIITEILE
>Mature_392_residues
TEGRKPHINVGTIGHVDHGKTTLTAALTTVLARKLSGANKVVKYDEIDKAPEEKARGITISTAHVEYETEGRHYAHVDCP
GHADYIKNMITGAAQMDVAILVVSATDGAMPQTREHILLAKQVGVKDIVVWINKCDVVEDEEMLSLVDMEIRELLSQYGY
DGDSIDAVRGSAVKALEEDADGPWSDKIMELVGALEKIELPMREKDKPFLMSVEDVFSIPGRGTVVTGRIERGVVRVGDK
IDIVGLRELQSTVCTGVEMFHKALEAGEAGDNAGILLRGIKKEDVERGQVLSAPGQMKSYKKFKAEVYVLKKEEGGRHTP
FFANYQPQFYVRTTDVTGSISLPAGVEMVMPGDNLSIEVALDKPVAIDKGLRFAVREGGRTVGSGIITEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=388, Percent_Identity=54.1237113402062, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=29.8876404494382, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=30.2483069977427, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=28.4064665127021, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=28.4064665127021, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI54607086, Length=319, Percent_Identity=29.4670846394984, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=28.2738095238095, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=28.2738095238095, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=28.2738095238095, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=27.5147928994083, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI4503507, Length=279, Percent_Identity=28.673835125448, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI53729339, Length=240, Percent_Identity=30.4166666666667, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI53729337, Length=240, Percent_Identity=30.4166666666667, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI157426893, Length=240, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI94966754, Length=182, Percent_Identity=29.6703296703297, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=391, Percent_Identity=67.2634271099744, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1789737, Length=391, Percent_Identity=67.2634271099744, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI2367247, Length=376, Percent_Identity=29.2553191489362, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789108, Length=259, Percent_Identity=28.957528957529, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI48994988, Length=283, Percent_Identity=29.3286219081272, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556456, Length=394, Percent_Identity=50.253807106599, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=45.360824742268, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=29.5711060948081, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=29.5711060948081, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=27.0642201834862, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=345, Percent_Identity=28.4057971014493, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=26.7734553775744, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=26.7734553775744, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=350, Percent_Identity=28.2857142857143, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=40.2684563758389, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=27.4678111587983, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=60.253164556962, Blast_Score=471, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=30.4740406320542, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=30.4740406320542, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=337, Percent_Identity=27.0029673590504, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=27.6967930029155, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=25.9786476868327, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=29.7520661157025, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=57.0332480818414, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=57.0332480818414, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=47.3007712082262, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45553807, Length=443, Percent_Identity=30.4740406320542, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI45553816, Length=443, Percent_Identity=30.4740406320542, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24651721, Length=443, Percent_Identity=30.4740406320542, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI17864154, Length=443, Percent_Identity=30.4740406320542, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24652838, Length=443, Percent_Identity=30.0225733634311, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137572, Length=443, Percent_Identity=30.0225733634311, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45550900, Length=433, Percent_Identity=28.8683602771363, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=30.2052785923754, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922690, Length=309, Percent_Identity=29.126213592233, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647048, Length=364, Percent_Identity=26.6483516483516, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647046, Length=364, Percent_Identity=26.6483516483516, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=189, Percent_Identity=35.978835978836, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42859; Mature: 42727

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGRKPHINVGTIGHVDHGKTTLTAALTTVLARKLSGANKVVKYDEIDKAPEEKARGIT
CCCCCCCEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCEE
ISTAHVEYETEGRHYAHVDCPGHADYIKNMITGAAQMDVAILVVSATDGAMPQTREHILL
EEEEEEEEECCCCEEEEEECCCHHHHHHHHHCCCHHCEEEEEEEECCCCCCCCCHHHEEE
AKQVGVKDIVVWINKCDVVEDEEMLSLVDMEIRELLSQYGYDGDSIDAVRGSAVKALEED
HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHC
ADGPWSDKIMELVGALEKIELPMREKDKPFLMSVEDVFSIPGRGTVVTGRIERGVVRVGD
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEECCCCEEEECC
KIDIVGLRELQSTVCTGVEMFHKALEAGEAGDNAGILLRGIKKEDVERGQVLSAPGQMKS
EEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCEECCCCHHHH
YKKFKAEVYVLKKEEGGRHTPFFANYQPQFYVRTTDVTGSISLPAGVEMVMPGDNLSIEV
HHHHEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEECCCCEEEEECCCCCEEEE
ALDKPVAIDKGLRFAVREGGRTVGSGIITEILE
EECCCEECCCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TEGRKPHINVGTIGHVDHGKTTLTAALTTVLARKLSGANKVVKYDEIDKAPEEKARGIT
CCCCCCEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCEE
ISTAHVEYETEGRHYAHVDCPGHADYIKNMITGAAQMDVAILVVSATDGAMPQTREHILL
EEEEEEEEECCCCEEEEEECCCHHHHHHHHHCCCHHCEEEEEEEECCCCCCCCCHHHEEE
AKQVGVKDIVVWINKCDVVEDEEMLSLVDMEIRELLSQYGYDGDSIDAVRGSAVKALEED
HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHC
ADGPWSDKIMELVGALEKIELPMREKDKPFLMSVEDVFSIPGRGTVVTGRIERGVVRVGD
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEECCCCEEEECC
KIDIVGLRELQSTVCTGVEMFHKALEAGEAGDNAGILLRGIKKEDVERGQVLSAPGQMKS
EEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCEECCCCHHHH
YKKFKAEVYVLKKEEGGRHTPFFANYQPQFYVRTTDVTGSISLPAGVEMVMPGDNLSIEV
HHHHEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEECCCCEEEEECCCCCEEEE
ALDKPVAIDKGLRFAVREGGRTVGSGIITEILE
EECCCEECCCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA