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The map label for this gene is tsf

Identifier: 88607503

GI number: 88607503

Start: 410498

End: 411361

Strand: Direct

Name: tsf

Synonym: APH_0398

Alternate gene names: 88607503

Gene position: 410498-411361 (Clockwise)

Preceding gene: 88606909

Following gene: 88606800

Centisome position: 27.9

GC content: 44.21

Gene sequence:

>864_bases
ATGAAAATTGATGTTGAGGTTATCAAAGAGTTGCGTCAGATAACTGGTGCGGGAATTGGTGACTGTAAGGAGGCTCTTGA
ATCATGTTCCGGTGATATGGATAAGGCTAGAGAGTATCTCAGAGAAAAGGGTTTATCTAAGGCATACAAGAAATCTCATA
GAGATGTTGCGGATGGGCTAGTTGCGGTTTTTGCTGAGGGTGATGTGGGTGCGATACTGAAATTGGGTTCAGAGACTGAT
TTCGTTGCTCGTAATGAGAAATTTCGCTCATTGGCTCTTGGTCTTGTAAGGGGGCTTTATGGTTATGGTGTGGAGGATTT
GGAGGGATTTTCTGCTAGTAGCTACGATGGGTCTCGTGTTGCTGATGAGATAGTGGCTGCTGCTGCTGTTTTGGGAGAGA
ATGTAGTCCTGAGTGGTGTTGGGTTTGTAAAATTAAGTGGTCCTGGTGTCATAGGAAGCTATGTTCATGGTATGGTTAGC
GAAGGTTTGGGCAAGGCTGCTGCTCTAGTAGTATTGGAGGGTGGTCCTCATTCTGATGAATTGGCTAGTTTTGCTAAACA
GCTTGCTATGCATATTGTAGCTGCTAAGCCAGAGGCATTATCTATTGATACATTGAGTAGTGATGTTGTTGACCGTGAGA
GGGAACTTGTAGCGAGGCAGGTCGAAGCTCTTGGAAAGCCTGATTCTGTAGCGCAGAAGATCATAGAAGGTAGAATGCAG
AAGTTCTTTGAGGAGATGGTGCTATTAGAGCAGGTTTTTGTTATGGATGGTCAAACAAAGATTCGTGATCTTCTCGTTCA
AAAAGGGCAGAGTCTGAAGCATGAGATACGTATAGCTGCGTATAGGTTATTCGCAATTGCTTAG

Upstream 100 bases:

>100_bases
AAGTAGTTGCCGGGGGGTCTGTTGTTTCTGCCTCTGGCGGTGAAGATGCCGTTGTGCAAGAATAGTACTGATGCTTCTGC
TTATTTTTAAGGTGATGTAG

Downstream 100 bases:

>100_bases
ATGTTAGGGGGTGGGGCATTTGTTAGGAATGTCCCTGCTTGTTCTTTTGTGTTTGTAGGTAAGTGCGGAGCGGGTGTGTC
CGCATAGCGTCGTGTGTATT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKIDVEVIKELRQITGAGIGDCKEALESCSGDMDKAREYLREKGLSKAYKKSHRDVADGLVAVFAEGDVGAILKLGSETD
FVARNEKFRSLALGLVRGLYGYGVEDLEGFSASSYDGSRVADEIVAAAAVLGENVVLSGVGFVKLSGPGVIGSYVHGMVS
EGLGKAAALVVLEGGPHSDELASFAKQLAMHIVAAKPEALSIDTLSSDVVDRERELVARQVEALGKPDSVAQKIIEGRMQ
KFFEEMVLLEQVFVMDGQTKIRDLLVQKGQSLKHEIRIAAYRLFAIA

Sequences:

>Translated_287_residues
MKIDVEVIKELRQITGAGIGDCKEALESCSGDMDKAREYLREKGLSKAYKKSHRDVADGLVAVFAEGDVGAILKLGSETD
FVARNEKFRSLALGLVRGLYGYGVEDLEGFSASSYDGSRVADEIVAAAAVLGENVVLSGVGFVKLSGPGVIGSYVHGMVS
EGLGKAAALVVLEGGPHSDELASFAKQLAMHIVAAKPEALSIDTLSSDVVDRERELVARQVEALGKPDSVAQKIIEGRMQ
KFFEEMVLLEQVFVMDGQTKIRDLLVQKGQSLKHEIRIAAYRLFAIA
>Mature_287_residues
MKIDVEVIKELRQITGAGIGDCKEALESCSGDMDKAREYLREKGLSKAYKKSHRDVADGLVAVFAEGDVGAILKLGSETD
FVARNEKFRSLALGLVRGLYGYGVEDLEGFSASSYDGSRVADEIVAAAAVLGENVVLSGVGFVKLSGPGVIGSYVHGMVS
EGLGKAAALVVLEGGPHSDELASFAKQLAMHIVAAKPEALSIDTLSSDVVDRERELVARQVEALGKPDSVAQKIIEGRMQ
KFFEEMVLLEQVFVMDGQTKIRDLLVQKGQSLKHEIRIAAYRLFAIA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=226, Percent_Identity=32.3008849557522, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI291084500, Length=250, Percent_Identity=30.8, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI291084502, Length=90, Percent_Identity=38.8888888888889, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI291084498, Length=90, Percent_Identity=38.8888888888889, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1786366, Length=276, Percent_Identity=34.4202898550725, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17561440, Length=225, Percent_Identity=27.1111111111111, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921466, Length=212, Percent_Identity=29.2452830188679, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_ANAPZ (Q2GKU8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505003.1
- ProteinModelPortal:   Q2GKU8
- SMR:   Q2GKU8
- STRING:   Q2GKU8
- GeneID:   3930974
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0398
- NMPDR:   fig|212042.5.peg.383
- TIGR:   APH_0398
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   APHA212042:APH_0398-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30803; Mature: 30803

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDVEVIKELRQITGAGIGDCKEALESCSGDMDKAREYLREKGLSKAYKKSHRDVADGL
CCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VAVFAEGDVGAILKLGSETDFVARNEKFRSLALGLVRGLYGYGVEDLEGFSASSYDGSRV
HHHHCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHH
ADEIVAAAAVLGENVVLSGVGFVKLSGPGVIGSYVHGMVSEGLGKAAALVVLEGGPHSDE
HHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LASFAKQLAMHIVAAKPEALSIDTLSSDVVDRERELVARQVEALGKPDSVAQKIIEGRMQ
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KFFEEMVLLEQVFVMDGQTKIRDLLVQKGQSLKHEIRIAAYRLFAIA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIDVEVIKELRQITGAGIGDCKEALESCSGDMDKAREYLREKGLSKAYKKSHRDVADGL
CCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VAVFAEGDVGAILKLGSETDFVARNEKFRSLALGLVRGLYGYGVEDLEGFSASSYDGSRV
HHHHCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHH
ADEIVAAAAVLGENVVLSGVGFVKLSGPGVIGSYVHGMVSEGLGKAAALVVLEGGPHSDE
HHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LASFAKQLAMHIVAAKPEALSIDTLSSDVVDRERELVARQVEALGKPDSVAQKIIEGRMQ
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KFFEEMVLLEQVFVMDGQTKIRDLLVQKGQSLKHEIRIAAYRLFAIA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA