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The map label for this gene is lipA

Identifier: 88607495

GI number: 88607495

Start: 360960

End: 361847

Strand: Direct

Name: lipA

Synonym: APH_0367

Alternate gene names: 88607495

Gene position: 360960-361847 (Clockwise)

Preceding gene: 88606771

Following gene: 88606988

Centisome position: 24.53

GC content: 47.41

Gene sequence:

>888_bases
ATGAATAGTAAGCCCAGCTGGTTAAGGGTAAAAATGCCCGGGGGCGAGGTATTTGAAGAAGTTAGAGATCTAGTCAAACG
CCAAAGGCTCAACACAGTATGTGAAGAGGCTGCATGTCCTAATATAGGTGAGTGCTGGAATAAGAGGCATGCTACTATAA
TGGTGATGGGTGACGTGTGTACCAGAGCGTGTGCGTTCTGTAATGTTAAGACTGGTGTTCCGAGAGCGTTGGATCTTGGG
GAGCCTGAGAGAGTGGGCGAAGCTATCAGCAAGTTAGGGTTGAAGCACGTGGTTATCACGTCTGTGGATAGGGATGATCT
ACCAGATGGTGGGGCAAGTCACTTTGCTAAGTGTATTAGAGAAATAAGGAAGCGTGACCCAAATGTGACCATAGAGATCC
TAACACCGGATTTTCAGGGTAAGCCAGGCGCGGTGGATGTCATCGCTTCTGCAAGGCCTGATGTATATAATCACAACTTG
GAGACGGTTCCTAGGTTATATGCAAGGGTGCGTCCTAGAGCTAAGTATTTCAATTCTTTGCAATTGTTGCAGCAAGTAAA
GGATAAGACTCAGGGAGTATTCACGAAGTCGGGATTGATGTTAGGGCTTGGTGAGCAGAAGGAAGAGGTATACCAGGTTA
TGGATGATCTCAGGTGTGCGGGGGTTGACTTTCTTGTCTTGGGGCAGTATTTGCAACCTACGAAGGACAACATTGATGTG
GATCGGTATGTTACCCCTGAGGAGTTTGAACAGTACAAGCGTATGGCATATGCCAAGGGTTTTTCTATGGTAGCATCTAG
TCCGTTAGCTAGGTCCTCATACCATGCTGAGGATGACTTTCTGAGGCTTCGCGCTTTACGCACTGCGCGTATGCGTGTTG
CTACTTAG

Upstream 100 bases:

>100_bases
TGCCAAAGTACGTGCTTGGTAATCTGCAGCACAGTGTCTGTGAAATGTTGACAAATTATGTAATTTTTACTACTCTCCCA
GCAGTTTATTACTGGTAGTT

Downstream 100 bases:

>100_bases
CTAGTAAGAAGTTGCCGGCAGAATAAGTTTATGCACTCGTAGCTCAGTTAGGATAGAGCGCTACCCTCCGGAGGTAGAGG
TCGCAGGTTCGAGTCCTGCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNSKPSWLRVKMPGGEVFEEVRDLVKRQRLNTVCEEAACPNIGECWNKRHATIMVMGDVCTRACAFCNVKTGVPRALDLG
EPERVGEAISKLGLKHVVITSVDRDDLPDGGASHFAKCIREIRKRDPNVTIEILTPDFQGKPGAVDVIASARPDVYNHNL
ETVPRLYARVRPRAKYFNSLQLLQQVKDKTQGVFTKSGLMLGLGEQKEEVYQVMDDLRCAGVDFLVLGQYLQPTKDNIDV
DRYVTPEEFEQYKRMAYAKGFSMVASSPLARSSYHAEDDFLRLRALRTARMRVAT

Sequences:

>Translated_295_residues
MNSKPSWLRVKMPGGEVFEEVRDLVKRQRLNTVCEEAACPNIGECWNKRHATIMVMGDVCTRACAFCNVKTGVPRALDLG
EPERVGEAISKLGLKHVVITSVDRDDLPDGGASHFAKCIREIRKRDPNVTIEILTPDFQGKPGAVDVIASARPDVYNHNL
ETVPRLYARVRPRAKYFNSLQLLQQVKDKTQGVFTKSGLMLGLGEQKEEVYQVMDDLRCAGVDFLVLGQYLQPTKDNIDV
DRYVTPEEFEQYKRMAYAKGFSMVASSPLARSSYHAEDDFLRLRALRTARMRVAT
>Mature_295_residues
MNSKPSWLRVKMPGGEVFEEVRDLVKRQRLNTVCEEAACPNIGECWNKRHATIMVMGDVCTRACAFCNVKTGVPRALDLG
EPERVGEAISKLGLKHVVITSVDRDDLPDGGASHFAKCIREIRKRDPNVTIEILTPDFQGKPGAVDVIASARPDVYNHNL
ETVPRLYARVRPRAKYFNSLQLLQQVKDKTQGVFTKSGLMLGLGEQKEEVYQVMDDLRCAGVDFLVLGQYLQPTKDNIDV
DRYVTPEEFEQYKRMAYAKGFSMVASSPLARSSYHAEDDFLRLRALRTARMRVAT

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=47.719298245614, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=48.3606557377049, Blast_Score=242, Evalue=3e-64,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=49.6376811594203, Blast_Score=295, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=48.4496124031008, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324770, Length=284, Percent_Identity=47.5352112676056, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=47.887323943662, Blast_Score=274, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_ANAPZ (Q2GKX7)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504974.1
- STRING:   Q2GKX7
- GeneID:   3931160
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0367
- NMPDR:   fig|212042.5.peg.351
- TIGR:   APH_0367
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   KQIFLER
- PhylomeDB:   Q2GKX7
- ProtClustDB:   PRK05481
- BioCyc:   APHA212042:APH_0367-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33255; Mature: 33255

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKPSWLRVKMPGGEVFEEVRDLVKRQRLNTVCEEAACPNIGECWNKRHATIMVMGDVC
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEHHHH
TRACAFCNVKTGVPRALDLGEPERVGEAISKLGLKHVVITSVDRDDLPDGGASHFAKCIR
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
EIRKRDPNVTIEILTPDFQGKPGAVDVIASARPDVYNHNLETVPRLYARVRPRAKYFNSL
HHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHHCHHHHHHHHH
QLLQQVKDKTQGVFTKSGLMLGLGEQKEEVYQVMDDLRCAGVDFLVLGQYLQPTKDNIDV
HHHHHHHHHHCCEEECCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
DRYVTPEEFEQYKRMAYAKGFSMVASSPLARSSYHAEDDFLRLRALRTARMRVAT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSKPSWLRVKMPGGEVFEEVRDLVKRQRLNTVCEEAACPNIGECWNKRHATIMVMGDVC
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEHHHH
TRACAFCNVKTGVPRALDLGEPERVGEAISKLGLKHVVITSVDRDDLPDGGASHFAKCIR
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
EIRKRDPNVTIEILTPDFQGKPGAVDVIASARPDVYNHNLETVPRLYARVRPRAKYFNSL
HHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHHCHHHHHHHHH
QLLQQVKDKTQGVFTKSGLMLGLGEQKEEVYQVMDDLRCAGVDFLVLGQYLQPTKDNIDV
HHHHHHHHHHCCEEECCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
DRYVTPEEFEQYKRMAYAKGFSMVASSPLARSSYHAEDDFLRLRALRTARMRVAT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA