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The map label for this gene is pleD [H]

Identifier: 88607360

GI number: 88607360

Start: 576134

End: 577504

Strand: Reverse

Name: pleD [H]

Synonym: APH_0551

Alternate gene names: 88607360

Gene position: 577504-576134 (Counterclockwise)

Preceding gene: 88607920

Following gene: 88607383

Centisome position: 39.25

GC content: 39.17

Gene sequence:

>1371_bases
GTGACTGCTAAAATACTAATAGTGGATGACTTGCCTTCCAACATTAAATTATTACAAGCGAAGCTTACAGCGGAATACTA
TACTGTAATTACTGCCACAAGTGGCCCTGAAGCAATAAGGATGGCAGAGGAACATCAACCAGATTTGATACTTCTCGACG
TCATGATGCCAGGAATGGACGGGTATGAGACGTGCAAAAACATGCGTACAAACCCTGCCATTGCGTATATACCTATAGTA
ATGGTTACAGCTTTAGATAATACTACAAGTAATAGGGTTAATGGGCTGAGTGCTGGTGCAGATGATTTTCTGATAAAGCC
TATAGATGATGTTGCGCTATTTTCCAGAATCAAGTCCCTTACTCGGTTCAAGATATTAGTAGATGAGCTAAGGCTTAGGG
GCAAGACTACTAAAGATATGAGCATGGTAGATTATAATATCATGCATTATGCAAATCAGATTTCTGATGGTAAGATTCTT
ATAATTGATGAAGACAAAATCCGAGCTGAAAGCATAAATAATCTCTTACAGGAAAGCTTCCAAGAGACTACTATACTCAG
CGACCCTACCCAGAACATAAATTTCACCAACGCGGCAAAGTATGATTTACTCATTATAGATATGCACTTTGCCGGAGATG
GGTTAAGACTTTGCTCAGAGTTCAGAAACAGCAATGAAGAGTCACGTTATACATCTATATTATTGCTCATCGATGAAAGC
GAAGACACAAATATATTGCATAAGGCGTTTGACATAGGCATTAGCGATTACATCATGGTTCCGATTTGTAATAACGAACT
TGTTGCCAGAGTAAACCTACAAATCAAAAAGAAAAGATATCAAAATGCCTTAAGGTCACACCTAAAGAACAATGCAGAAA
TGTCTATAAGGGATTCTCTTACAGGATGCTATAACAGGCGTTACTTCGAGATGCACTTCCAAAATATACTTAATGAGTCA
CAAGAAAAAGACAAACCTCTCTCCGTAATGCTGCTGGATATTGACCATTTTAAGCAGGTAAATGACTCTTTGGGCCATCA
GGTCGGAGATGAAATACTGCAGCAGATATGTAAACGAATCTTCAATAGCATAAGAATATCCGATCTGCTAGCAAGGTATG
GCGGAGAGGAATTTGTTATAATTATGCCTGAAACATGTATAGAAGAGGCTACGTTAGTTGCAGACAGGCTACGACGCATA
ATAGCAGAATCTCCCTTTACTACAAGTGCAGGTCCTTTAAACAAAACTTTAAGCATAGGAGCGGCCAACTTGAGAAGCGG
AGAGACGGCTGAACAGCTACTTACTCGAGCGGATAAGTTGCTTTACGTTGCAAAAAAAACTAGAAACTCGGTTGTGTCTG
AAAAAATCTAA

Upstream 100 bases:

>100_bases
TATCTATTTCTTCTACACTTGTAAATCTTATATGAAGCAAGACTATAGTGTCAGGTACGATTCATCGTATTGTGCAAGAC
GGTAAAATAGTGAGTGTAAT

Downstream 100 bases:

>100_bases
ATTTATTCTCGTTGGGATGATCGTCTCAACTTCCTTGGCTTTTAAAAAACCGCGTGATAAACGCCCTCTCGCATTCAATT
AGCATCAAACTCTTTACCAC

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTAKILIVDDLPSNIKLLQAKLTAEYYTVITATSGPEAIRMAEEHQPDLILLDVMMPGMDGYETCKNMRTNPAIAYIPIV
MVTALDNTTSNRVNGLSAGADDFLIKPIDDVALFSRIKSLTRFKILVDELRLRGKTTKDMSMVDYNIMHYANQISDGKIL
IIDEDKIRAESINNLLQESFQETTILSDPTQNINFTNAAKYDLLIIDMHFAGDGLRLCSEFRNSNEESRYTSILLLIDES
EDTNILHKAFDIGISDYIMVPICNNELVARVNLQIKKKRYQNALRSHLKNNAEMSIRDSLTGCYNRRYFEMHFQNILNES
QEKDKPLSVMLLDIDHFKQVNDSLGHQVGDEILQQICKRIFNSIRISDLLARYGGEEFVIIMPETCIEEATLVADRLRRI
IAESPFTTSAGPLNKTLSIGAANLRSGETAEQLLTRADKLLYVAKKTRNSVVSEKI

Sequences:

>Translated_456_residues
MTAKILIVDDLPSNIKLLQAKLTAEYYTVITATSGPEAIRMAEEHQPDLILLDVMMPGMDGYETCKNMRTNPAIAYIPIV
MVTALDNTTSNRVNGLSAGADDFLIKPIDDVALFSRIKSLTRFKILVDELRLRGKTTKDMSMVDYNIMHYANQISDGKIL
IIDEDKIRAESINNLLQESFQETTILSDPTQNINFTNAAKYDLLIIDMHFAGDGLRLCSEFRNSNEESRYTSILLLIDES
EDTNILHKAFDIGISDYIMVPICNNELVARVNLQIKKKRYQNALRSHLKNNAEMSIRDSLTGCYNRRYFEMHFQNILNES
QEKDKPLSVMLLDIDHFKQVNDSLGHQVGDEILQQICKRIFNSIRISDLLARYGGEEFVIIMPETCIEEATLVADRLRRI
IAESPFTTSAGPLNKTLSIGAANLRSGETAEQLLTRADKLLYVAKKTRNSVVSEKI
>Mature_455_residues
TAKILIVDDLPSNIKLLQAKLTAEYYTVITATSGPEAIRMAEEHQPDLILLDVMMPGMDGYETCKNMRTNPAIAYIPIVM
VTALDNTTSNRVNGLSAGADDFLIKPIDDVALFSRIKSLTRFKILVDELRLRGKTTKDMSMVDYNIMHYANQISDGKILI
IDEDKIRAESINNLLQESFQETTILSDPTQNINFTNAAKYDLLIIDMHFAGDGLRLCSEFRNSNEESRYTSILLLIDESE
DTNILHKAFDIGISDYIMVPICNNELVARVNLQIKKKRYQNALRSHLKNNAEMSIRDSLTGCYNRRYFEMHFQNILNESQ
EKDKPLSVMLLDIDHFKQVNDSLGHQVGDEILQQICKRIFNSIRISDLLARYGGEEFVIIMPETCIEEATLVADRLRRII
AESPFTTSAGPLNKTLSIGAANLRSGETAEQLLTRADKLLYVAKKTRNSVVSEKI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=36.5384615384615, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=40.2298850574713, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=34.3023255813954, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082007, Length=206, Percent_Identity=32.0388349514563, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=200, Percent_Identity=32, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=33.7078651685393, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787816, Length=150, Percent_Identity=34.6666666666667, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=36.4197530864198, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=28.2208588957055, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788394, Length=185, Percent_Identity=31.8918918918919, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=172, Percent_Identity=29.0697674418605, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=36.1344537815126, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=33.9393939393939, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=31.0924369747899, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=34.4262295081967, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=35.5371900826446, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790437, Length=106, Percent_Identity=42.4528301886792, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=29.559748427673, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787541, Length=151, Percent_Identity=29.8013245033113, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=106, Percent_Identity=35.8490566037736, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI2367329, Length=119, Percent_Identity=40.3361344537815, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51571; Mature: 51440

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKILIVDDLPSNIKLLQAKLTAEYYTVITATSGPEAIRMAEEHQPDLILLDVMMPGMD
CCEEEEEEECCCCCHHHHHHHHCEEEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCC
GYETCKNMRTNPAIAYIPIVMVTALDNTTSNRVNGLSAGADDFLIKPIDDVALFSRIKSL
HHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHH
TRFKILVDELRLRGKTTKDMSMVDYNIMHYANQISDGKILIIDEDKIRAESINNLLQESF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
QETTILSDPTQNINFTNAAKYDLLIIDMHFAGDGLRLCSEFRNSNEESRYTSILLLIDES
HHCEECCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEEEEECC
EDTNILHKAFDIGISDYIMVPICNNELVARVNLQIKKKRYQNALRSHLKNNAEMSIRDSL
CCCHHHHHHHHCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEHHHHH
TGCYNRRYFEMHFQNILNESQEKDKPLSVMLLDIDHFKQVNDSLGHQVGDEILQQICKRI
HHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNSIRISDLLARYGGEEFVIIMPETCIEEATLVADRLRRIIAESPFTTSAGPLNKTLSIG
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC
AANLRSGETAEQLLTRADKLLYVAKKTRNSVVSEKI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAKILIVDDLPSNIKLLQAKLTAEYYTVITATSGPEAIRMAEEHQPDLILLDVMMPGMD
CEEEEEEECCCCCHHHHHHHHCEEEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCC
GYETCKNMRTNPAIAYIPIVMVTALDNTTSNRVNGLSAGADDFLIKPIDDVALFSRIKSL
HHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHH
TRFKILVDELRLRGKTTKDMSMVDYNIMHYANQISDGKILIIDEDKIRAESINNLLQESF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
QETTILSDPTQNINFTNAAKYDLLIIDMHFAGDGLRLCSEFRNSNEESRYTSILLLIDES
HHCEECCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEEEEECC
EDTNILHKAFDIGISDYIMVPICNNELVARVNLQIKKKRYQNALRSHLKNNAEMSIRDSL
CCCHHHHHHHHCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEHHHHH
TGCYNRRYFEMHFQNILNESQEKDKPLSVMLLDIDHFKQVNDSLGHQVGDEILQQICKRI
HHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNSIRISDLLARYGGEEFVIIMPETCIEEATLVADRLRRIIAESPFTTSAGPLNKTLSIG
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC
AANLRSGETAEQLLTRADKLLYVAKKTRNSVVSEKI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA