Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 88607345

GI number: 88607345

Start: 142791

End: 144824

Strand: Reverse

Name: ligA

Synonym: APH_0138

Alternate gene names: 88607345

Gene position: 144824-142791 (Counterclockwise)

Preceding gene: 88607392

Following gene: 88606743

Centisome position: 9.84

GC content: 43.76

Gene sequence:

>2034_bases
ATGTCTGACAACGACAGTCGGCGGCGTCTTGCTGATCTAAATGCGCAGCTGAAGCTTCACGATGTTCTATATCATGAACA
TGATGCACCGGAGATCTCAGATGCTCAGTATGATGCGCTCGTGCAGGAGAAACGTGAGTTATTGGAAAAGTTCCCCGAGC
TTAGCGCCTATAATGATTATGAAGGAGTAATTGGTGCACTCACAGTAGATGCCAGATTGCCCAAGATTGCCCATCGTGAA
CCCATGTTATCTCTGGAAAATTCCTTTACTATACAGGACGTAGAAAAGTTTATCTCCAGGGTAAAGCGTTCACTTAATAT
GGATCCTGAAGTCTCTATCACCATAGCTTGTGAACCTAAAATTGATGGTTTGTCATTTGCTGCATTATACGAAAAGGGCT
CTCTTATACGGGTTGCAACTAGAGGAAATGGCCATTTAGGGGAAGATATTACGAACACCGCAAAAGTAATACGTAAGCTC
CCTCACAAAATCGCAAATGCTCCTGAAGTTCTGGAAGTCCGAGGTGAAATATACATGCACCACAGCGACTTTGAAAAACT
GAAGGACGTATGTAACTTTGCGAATCCACGCAATGCCGCAGCTGGCTCTATCCGTCAACTAAATCCTAAAATTGCAGAAG
AGCGGAATCTTCGCTATGTGGCATATTGTATAGTCAATAGCGCCTTAGCTTCTCAAGAAGCAATATTAAAACAGCTTGCC
GAATGGGGGTTTTGCACTCATACAGAGGTACTTTTCGCTGATAATATGGACGACGCGCTGTCGTTCCATACCAGAATGTA
TAATACACGCAGTACTCTAGGGTATGACATTGATGGAATAGTGTATAAAGTAAATGATACGCACCTACAAAAACTGCTTG
GCTCCACAAGTAAGTATCCTAGATGGGCCACAGCTCATAAGTTTCCTTCAACTGAGGCCATCACTAAATTGCGTGATATT
TCTGTGCAGGTTGGAAGAACAGGAGTTATAACGCCCATAGCGGAATTGGAGCCCATAAATATAGGGGGCACCCTGGTATC
GCGGGCAAGTCTTCATAACCTTAACGAGATTGCCCGTAAAGATATACGTATTGGCGACAGTGTTATAGTAAAAAGGGCTG
GCGAGGTCATACCACAGGTAGTAGGCGTAGACCATACCGCACGTTGTAATTCTGCTGTGCCTGAAGAGTATGTATTCCCC
TCGCATTGTCCATCATGTGGAAGTACACTTAGTAGAGCACCTGGAGAAGTAGCGATGCGCTGCACGGCTGAATTATCATG
TCAAGCGCAGGTGCTGGAGCGAGTGAAGCACTTTGTATCTCGGGATGGGCTAAATATAGTAGGTCTAGGAGAAAAACAAA
TAGAGTTTTTCTGCAATGCATCATACATCAGCAACGTTGCTGACATTTTTTCGCTCAGAGAAAAGATAAGTCACATGAAC
TTAAGCGCTGAACATGGATGGGGAGAGAAGTCTATAGCCCTGCTCATTAACGCTATCAATGCCAGTACCACTGTGAAACT
GAGTAATTTCATTTTTGCCTTGGGAATCAGGTTCATAGGAAAGGGTGCCGCAAAGCTGATCGCGGAACACTATAGATCTT
ATAGCGCCTGGGTACGTGCCATGACATCACTGGCGAATGGAGAAGATCCTGATAATATACATGGTATTGGCCTCAAAAGT
ATTGAGTCCTTACGTGCGTTTTTCTCAAGTGAAGACAATTTGAGAGTACTCCAAACGCTTGAAGAAAAACTCAATATCTT
GAATGAAATCGCAAATACAGAGACTGCGTCTCCCATTTCTGGAAAGACTATTGTATTCACTGGCGTGTTGGAAGATATGT
CCCGTAACGAAGCTGCGAAATATGCGGAAACTCTAGGGGCTAAAGTTGGGAATACTGTAACTACTAAAACTGACATACTT
GTTGCTGGAAGCAACTCAGGAAGTAAGCTAGACACTGCTAGAAAACTCGGTATTCAGGTTATGAATGAGTCGGAGTGGAA
AGATCTACTGAAAACTGTATCTAACTCCGAGTAA

Upstream 100 bases:

>100_bases
GCGAGTTCATCGGAGGATGTGACATAGTACGTGAAATGTACCAAAGCGGTGAACTACAAGAGCTTTTGAAAAAGAAGGGC
GTGATAACTGGATAGGTCAC

Downstream 100 bases:

>100_bases
TAGCCTACTTTCGTAATGTCTTCTTATTTACTATGGAGCATATGGTGCTGTGTGCATCTAAAAAATCCGCAAATCAAGAG
CCGTCGCTTACGTTAGCCGC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MSDNDSRRRLADLNAQLKLHDVLYHEHDAPEISDAQYDALVQEKRELLEKFPELSAYNDYEGVIGALTVDARLPKIAHRE
PMLSLENSFTIQDVEKFISRVKRSLNMDPEVSITIACEPKIDGLSFAALYEKGSLIRVATRGNGHLGEDITNTAKVIRKL
PHKIANAPEVLEVRGEIYMHHSDFEKLKDVCNFANPRNAAAGSIRQLNPKIAEERNLRYVAYCIVNSALASQEAILKQLA
EWGFCTHTEVLFADNMDDALSFHTRMYNTRSTLGYDIDGIVYKVNDTHLQKLLGSTSKYPRWATAHKFPSTEAITKLRDI
SVQVGRTGVITPIAELEPINIGGTLVSRASLHNLNEIARKDIRIGDSVIVKRAGEVIPQVVGVDHTARCNSAVPEEYVFP
SHCPSCGSTLSRAPGEVAMRCTAELSCQAQVLERVKHFVSRDGLNIVGLGEKQIEFFCNASYISNVADIFSLREKISHMN
LSAEHGWGEKSIALLINAINASTTVKLSNFIFALGIRFIGKGAAKLIAEHYRSYSAWVRAMTSLANGEDPDNIHGIGLKS
IESLRAFFSSEDNLRVLQTLEEKLNILNEIANTETASPISGKTIVFTGVLEDMSRNEAAKYAETLGAKVGNTVTTKTDIL
VAGSNSGSKLDTARKLGIQVMNESEWKDLLKTVSNSE

Sequences:

>Translated_677_residues
MSDNDSRRRLADLNAQLKLHDVLYHEHDAPEISDAQYDALVQEKRELLEKFPELSAYNDYEGVIGALTVDARLPKIAHRE
PMLSLENSFTIQDVEKFISRVKRSLNMDPEVSITIACEPKIDGLSFAALYEKGSLIRVATRGNGHLGEDITNTAKVIRKL
PHKIANAPEVLEVRGEIYMHHSDFEKLKDVCNFANPRNAAAGSIRQLNPKIAEERNLRYVAYCIVNSALASQEAILKQLA
EWGFCTHTEVLFADNMDDALSFHTRMYNTRSTLGYDIDGIVYKVNDTHLQKLLGSTSKYPRWATAHKFPSTEAITKLRDI
SVQVGRTGVITPIAELEPINIGGTLVSRASLHNLNEIARKDIRIGDSVIVKRAGEVIPQVVGVDHTARCNSAVPEEYVFP
SHCPSCGSTLSRAPGEVAMRCTAELSCQAQVLERVKHFVSRDGLNIVGLGEKQIEFFCNASYISNVADIFSLREKISHMN
LSAEHGWGEKSIALLINAINASTTVKLSNFIFALGIRFIGKGAAKLIAEHYRSYSAWVRAMTSLANGEDPDNIHGIGLKS
IESLRAFFSSEDNLRVLQTLEEKLNILNEIANTETASPISGKTIVFTGVLEDMSRNEAAKYAETLGAKVGNTVTTKTDIL
VAGSNSGSKLDTARKLGIQVMNESEWKDLLKTVSNSE
>Mature_676_residues
SDNDSRRRLADLNAQLKLHDVLYHEHDAPEISDAQYDALVQEKRELLEKFPELSAYNDYEGVIGALTVDARLPKIAHREP
MLSLENSFTIQDVEKFISRVKRSLNMDPEVSITIACEPKIDGLSFAALYEKGSLIRVATRGNGHLGEDITNTAKVIRKLP
HKIANAPEVLEVRGEIYMHHSDFEKLKDVCNFANPRNAAAGSIRQLNPKIAEERNLRYVAYCIVNSALASQEAILKQLAE
WGFCTHTEVLFADNMDDALSFHTRMYNTRSTLGYDIDGIVYKVNDTHLQKLLGSTSKYPRWATAHKFPSTEAITKLRDIS
VQVGRTGVITPIAELEPINIGGTLVSRASLHNLNEIARKDIRIGDSVIVKRAGEVIPQVVGVDHTARCNSAVPEEYVFPS
HCPSCGSTLSRAPGEVAMRCTAELSCQAQVLERVKHFVSRDGLNIVGLGEKQIEFFCNASYISNVADIFSLREKISHMNL
SAEHGWGEKSIALLINAINASTTVKLSNFIFALGIRFIGKGAAKLIAEHYRSYSAWVRAMTSLANGEDPDNIHGIGLKSI
ESLRAFFSSEDNLRVLQTLEEKLNILNEIANTETASPISGKTIVFTGVLEDMSRNEAAKYAETLGAKVGNTVTTKTDILV
AGSNSGSKLDTARKLGIQVMNESEWKDLLKTVSNSE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=680, Percent_Identity=40.4411764705882, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI87082305, Length=536, Percent_Identity=21.455223880597, Blast_Score=93, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ANAPZ (Q2GLI8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504763.1
- ProteinModelPortal:   Q2GLI8
- STRING:   Q2GLI8
- GeneID:   3929997
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0138
- NMPDR:   fig|212042.5.peg.133
- TIGR:   APH_0138
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   FHELKAL
- ProtClustDB:   CLSK747278
- BioCyc:   APHA212042:APH_0138-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74769; Mature: 74638

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 120-120 BINDING 118-118 BINDING 141-141 BINDING 176-176 BINDING 283-283 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNDSRRRLADLNAQLKLHDVLYHEHDAPEISDAQYDALVQEKRELLEKFPELSAYNDY
CCCCHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
EGVIGALTVDARLPKIAHREPMLSLENSFTIQDVEKFISRVKRSLNMDPEVSITIACEPK
CCCEEEEEECCCCCCHHCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
IDGLSFAALYEKGSLIRVATRGNGHLGEDITNTAKVIRKLPHKIANAPEVLEVRGEIYMH
CCCCEEHEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEECCCEEEE
HSDFEKLKDVCNFANPRNAAAGSIRQLNPKIAEERNLRYVAYCIVNSALASQEAILKQLA
CCHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
EWGFCTHTEVLFADNMDDALSFHTRMYNTRSTLGYDIDGIVYKVNDTHLQKLLGSTSKYP
HCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCC
RWATAHKFPSTEAITKLRDISVQVGRTGVITPIAELEPINIGGTLVSRASLHNLNEIARK
CCCHHCCCCCHHHHHHHHHCEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
DIRIGDSVIVKRAGEVIPQVVGVDHTARCNSAVPEEYVFPSHCPSCGSTLSRAPGEVAMR
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHEEH
CTAELSCQAQVLERVKHFVSRDGLNIVGLGEKQIEFFCNASYISNVADIFSLREKISHMN
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHEECHHHHHHHHHHHHHHHHHHHCC
LSAEHGWGEKSIALLINAINASTTVKLSNFIFALGIRFIGKGAAKLIAEHYRSYSAWVRA
CCCCCCCCCCCHHHEEECCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MTSLANGEDPDNIHGIGLKSIESLRAFFSSEDNLRVLQTLEEKLNILNEIANTETASPIS
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GKTIVFTGVLEDMSRNEAAKYAETLGAKVGNTVTTKTDILVAGSNSGSKLDTARKLGIQV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEEEECCCCCCHHHHHHHHHHHH
MNESEWKDLLKTVSNSE
CCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDNDSRRRLADLNAQLKLHDVLYHEHDAPEISDAQYDALVQEKRELLEKFPELSAYNDY
CCCHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
EGVIGALTVDARLPKIAHREPMLSLENSFTIQDVEKFISRVKRSLNMDPEVSITIACEPK
CCCEEEEEECCCCCCHHCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
IDGLSFAALYEKGSLIRVATRGNGHLGEDITNTAKVIRKLPHKIANAPEVLEVRGEIYMH
CCCCEEHEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEECCCEEEE
HSDFEKLKDVCNFANPRNAAAGSIRQLNPKIAEERNLRYVAYCIVNSALASQEAILKQLA
CCHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
EWGFCTHTEVLFADNMDDALSFHTRMYNTRSTLGYDIDGIVYKVNDTHLQKLLGSTSKYP
HCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCC
RWATAHKFPSTEAITKLRDISVQVGRTGVITPIAELEPINIGGTLVSRASLHNLNEIARK
CCCHHCCCCCHHHHHHHHHCEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
DIRIGDSVIVKRAGEVIPQVVGVDHTARCNSAVPEEYVFPSHCPSCGSTLSRAPGEVAMR
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHEEH
CTAELSCQAQVLERVKHFVSRDGLNIVGLGEKQIEFFCNASYISNVADIFSLREKISHMN
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHEECHHHHHHHHHHHHHHHHHHHCC
LSAEHGWGEKSIALLINAINASTTVKLSNFIFALGIRFIGKGAAKLIAEHYRSYSAWVRA
CCCCCCCCCCCHHHEEECCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MTSLANGEDPDNIHGIGLKSIESLRAFFSSEDNLRVLQTLEEKLNILNEIANTETASPIS
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GKTIVFTGVLEDMSRNEAAKYAETLGAKVGNTVTTKTDILVAGSNSGSKLDTARKLGIQV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEEEECCCCCCHHHHHHHHHHHH
MNESEWKDLLKTVSNSE
CCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA