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The map label for this gene is ispE

Identifier: 88607234

GI number: 88607234

Start: 601526

End: 602416

Strand: Reverse

Name: ispE

Synonym: APH_0574

Alternate gene names: 88607234

Gene position: 602416-601526 (Counterclockwise)

Preceding gene: 88606958

Following gene: 88607281

Centisome position: 40.94

GC content: 41.86

Gene sequence:

>891_bases
ATGTCGAAGTATTTTATTAGCGCGCCTGCGAAGATAAACTTGTTTCTCCATATAGTCGGTAAAGCTCCGTGTGGGTATCA
CTTAATAGAGTCTGTCTTTGCATTTGTAGAGTTATACGATGTTTTAGAGTTTGACATTGGCTCTAGAAACCGAGGGATAA
GGTTTCTCAAACCTTCGTGTATTAATAGACGTGATAACACTATACAGCGAGCTATTGGACATTTGGTGAGGAGATGCTCG
CCGGGCACTACGGATAATGTTTATGTTAAAGTTTTGAAGAATATTCCTGTATCGTCTGGATTGGCAGGCGGGTCAGCAGA
TGCCGCTGCTGCTATTAATCTGCTAAGCAAGCTGTGGGGCATAAATGAAAAAGAAACTGAGAGGGTAGCTTTTAGGGTTG
GAAGCGATGTTCCTGTGTGTTTAGAATCTAAAACTGCGTTTGTTGCGGGCATGGGAGACGTAGTGGAATTGCTAGAAGAC
TCATTCTTGCCTAGGCACGTTATTTTGGTAGGGCCCAAAGTGGAATTGAGCACAAAAAGTGTCTTTGACATGTACAATCC
CAAAGCGTTTTCTCCAAGTATAGGTAAGTTGCCTGGTAATTCTGAGGATTGGCTTTCTTTATTGAAGGAAGCTCGTAATG
ACTTAACGGAGGTTTCTGTAGAATTGGTACCGGAGATTCGTATCATATTGGATGTGCTGGGTTCATTAGATGGGTGTTGC
TTTTCCAGAATGAGTGGAAGTGGGGCTATGAGCTTCGCTATTTTTGAGGATGAGAATTCAGCTGAGTTAGCCACTAGGTA
TCTGAGGAGAAATTACCCAGACTGGTTTATTTTCAAAACTAGGATTATTAGGTCGAGTGATCAGGAAGATAATAATATGG
GTAATCTTTGA

Upstream 100 bases:

>100_bases
AGTGATAATTTTATTTAAATTTTTAATAATTTATTTACAATAATGCAAAATAGAGAGTATTACATGTTTGCGGGGATGGG
TTCTGTAGATCGGATGTATG

Downstream 100 bases:

>100_bases
GGCAGGGAATTTGTCGTGATGATTTATATACTCTTTTCTCCTCTTGATCTTGTAGCTTCCTTAATAAAACTAGGAGAGAG
AAACTAAGTCTCCTCTGCAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSKYFISAPAKINLFLHIVGKAPCGYHLIESVFAFVELYDVLEFDIGSRNRGIRFLKPSCINRRDNTIQRAIGHLVRRCS
PGTTDNVYVKVLKNIPVSSGLAGGSADAAAAINLLSKLWGINEKETERVAFRVGSDVPVCLESKTAFVAGMGDVVELLED
SFLPRHVILVGPKVELSTKSVFDMYNPKAFSPSIGKLPGNSEDWLSLLKEARNDLTEVSVELVPEIRIILDVLGSLDGCC
FSRMSGSGAMSFAIFEDENSAELATRYLRRNYPDWFIFKTRIIRSSDQEDNNMGNL

Sequences:

>Translated_296_residues
MSKYFISAPAKINLFLHIVGKAPCGYHLIESVFAFVELYDVLEFDIGSRNRGIRFLKPSCINRRDNTIQRAIGHLVRRCS
PGTTDNVYVKVLKNIPVSSGLAGGSADAAAAINLLSKLWGINEKETERVAFRVGSDVPVCLESKTAFVAGMGDVVELLED
SFLPRHVILVGPKVELSTKSVFDMYNPKAFSPSIGKLPGNSEDWLSLLKEARNDLTEVSVELVPEIRIILDVLGSLDGCC
FSRMSGSGAMSFAIFEDENSAELATRYLRRNYPDWFIFKTRIIRSSDQEDNNMGNL
>Mature_295_residues
SKYFISAPAKINLFLHIVGKAPCGYHLIESVFAFVELYDVLEFDIGSRNRGIRFLKPSCINRRDNTIQRAIGHLVRRCSP
GTTDNVYVKVLKNIPVSSGLAGGSADAAAAINLLSKLWGINEKETERVAFRVGSDVPVCLESKTAFVAGMGDVVELLEDS
FLPRHVILVGPKVELSTKSVFDMYNPKAFSPSIGKLPGNSEDWLSLLKEARNDLTEVSVELVPEIRIILDVLGSLDGCCF
SRMSGSGAMSFAIFEDENSAELATRYLRRNYPDWFIFKTRIIRSSDQEDNNMGNL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=283, Percent_Identity=30.3886925795053, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_ANAPZ (Q2GKD6)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505165.1
- ProteinModelPortal:   Q2GKD6
- SMR:   Q2GKD6
- STRING:   Q2GKD6
- GeneID:   3931014
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0574
- NMPDR:   fig|212042.5.peg.568
- TIGR:   APH_0574
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- PhylomeDB:   Q2GKD6
- ProtClustDB:   PRK14610
- BioCyc:   APHA212042:APH_0574-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32791; Mature: 32660

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYFISAPAKINLFLHIVGKAPCGYHLIESVFAFVELYDVLEFDIGSRNRGIRFLKPSC
CCCEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHH
INRRDNTIQRAIGHLVRRCSPGTTDNVYVKVLKNIPVSSGLAGGSADAAAAINLLSKLWG
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
INEKETERVAFRVGSDVPVCLESKTAFVAGMGDVVELLEDSFLPRHVILVGPKVELSTKS
CCHHHHHHEEEEECCCCCEEECCCCCEEECHHHHHHHHHHCCCCCEEEEECCCEEECHHH
VFDMYNPKAFSPSIGKLPGNSEDWLSLLKEARNDLTEVSVELVPEIRIILDVLGSLDGCC
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FSRMSGSGAMSFAIFEDENSAELATRYLRRNYPDWFIFKTRIIRSSDQEDNNMGNL
HHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKYFISAPAKINLFLHIVGKAPCGYHLIESVFAFVELYDVLEFDIGSRNRGIRFLKPSC
CCEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHH
INRRDNTIQRAIGHLVRRCSPGTTDNVYVKVLKNIPVSSGLAGGSADAAAAINLLSKLWG
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
INEKETERVAFRVGSDVPVCLESKTAFVAGMGDVVELLEDSFLPRHVILVGPKVELSTKS
CCHHHHHHEEEEECCCCCEEECCCCCEEECHHHHHHHHHHCCCCCEEEEECCCEEECHHH
VFDMYNPKAFSPSIGKLPGNSEDWLSLLKEARNDLTEVSVELVPEIRIILDVLGSLDGCC
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FSRMSGSGAMSFAIFEDENSAELATRYLRRNYPDWFIFKTRIIRSSDQEDNNMGNL
HHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA