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The map label for this gene is nuoF [H]

Identifier: 88607132

GI number: 88607132

Start: 448952

End: 450232

Strand: Direct

Name: nuoF [H]

Synonym: APH_0434

Alternate gene names: 88607132

Gene position: 448952-450232 (Clockwise)

Preceding gene: 88607717

Following gene: 88607226

Centisome position: 30.51

GC content: 45.59

Gene sequence:

>1281_bases
GTGAGAGACGAGGATTCTATATTTACTAATTTATCCGGAAAAGAAAGCCCTTATTTAGCGGCGGCAAAAAGCCGTGGTAC
GTGGTTTAACACTAAAGAGTTGCTGGCACTAGGGCGTGATAAAATCATTCAAGAAATTAAGGACTCAGGCCTCAGGGGGA
GAGGAGGAGCAGGGTTTCCCACAGGGCTTAAGTGGAGTTTTATGCCGAAAGCCTTGCCGGAGGGTTCTTCTGCGTATCTT
GTGGTAAACGCTGATGAGTCCGAGCCTGGTACGTGTAAGGACAGGGACATAATTAGGTATGATCCGCATAAAATTCTAGA
AGGCATACTTATTGCAGGTTTTGCTATGGGTGTGACTGCTGCTTACATTTACATTCGGGGTGAGTTTTATAATGAGTACA
CCGTTCTTTTCAAGGCTTTACAGGAAGCTTATGCAGATGGTCTATTAGGAGAAAATGCGTGTGGTTCTGGTTATAACTTG
AACGTTTTTATTCATAGGGGAGCGGGGGCATACATTTGCGGAGAAGAGAGTGCGCAGATTGAATCTATTGAGGGCAGGAA
AGGCATGCCCAGATTGAAGCCTCCATTTCCGGCTGCTGTGGGATTATACGGGTCGCCGACCACTGTAAATAATGTTGAGA
CTATTGCTGTAGTAGGGGATATCCTAAGAAGAGGTTCGGGGTGGTTCTCTTCGCTAGGTATACCGAATAATACTGGGACT
AAGGTCTTTTGTATATCTGGTCACGTGAACAATCCTTGCAATGTCGAGGAGGAATTGGGAATTCCCATGAGAGAACTCAT
TGAGAAGTATGCAGGGGGCGTAATAGGAGGTTGGGATAATTTGCTTGCCGTGATTCCTGGAGGGTCTTCTGTTCCCATGT
TGCCGAAGCACATATGCGACGATATAACTATGGATTTTGATTCTTTGAGGGCTGCTAAGTCTGGGTTAGGAACTGCTGGC
CTGATAGTTATGGATAAGTCTACTGATCTCATCGAAGCTATAGAGAGGATTTCGAGTTTTTATATGCATGAATCGTGTGG
TCAATGCAGCCCTTGTAGGGAAGGCACTGGTTGGATGTGGAGAATGATGCGAAAAATGCTCGATGGTACAGCAGAGGAGA
GTGAGATAGATAAGCTGCTAGATGTTGCTTCTCAGATAGAGGGGCATACAATTTGTGCGTTGGCGGATGCTGCGGTATGG
CCGATACAAGGTCTATTTCGTCATTTTAGATCTGTTGTAGAGGAGAGGCTAAGAAGTTCTAAGGCGCGACGTTTAGCTTA
A

Upstream 100 bases:

>100_bases
CAGGCTTGGTATGCAGTGTTCGTTGAAGTAAGTTGCGAACTTAAGAATTTGAGTTATTTCTGTACTTTAGTAATATAGAC
ATGGTCTTAGTGGGGGTATT

Downstream 100 bases:

>100_bases
TTACGTACTGCTTAGGAGCTGCGTTGAGGTGTTATGCCTAATACAATACAGATATTAGGTCTGTCTTCTCTTCTGAAGTT
AAGAGAGAGGTGAGTTGTAT

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRDEDSIFTNLSGKESPYLAAAKSRGTWFNTKELLALGRDKIIQEIKDSGLRGRGGAGFPTGLKWSFMPKALPEGSSAYL
VVNADESEPGTCKDRDIIRYDPHKILEGILIAGFAMGVTAAYIYIRGEFYNEYTVLFKALQEAYADGLLGENACGSGYNL
NVFIHRGAGAYICGEESAQIESIEGRKGMPRLKPPFPAAVGLYGSPTTVNNVETIAVVGDILRRGSGWFSSLGIPNNTGT
KVFCISGHVNNPCNVEEELGIPMRELIEKYAGGVIGGWDNLLAVIPGGSSVPMLPKHICDDITMDFDSLRAAKSGLGTAG
LIVMDKSTDLIEAIERISSFYMHESCGQCSPCREGTGWMWRMMRKMLDGTAEESEIDKLLDVASQIEGHTICALADAAVW
PIQGLFRHFRSVVEERLRSSKARRLA

Sequences:

>Translated_426_residues
MRDEDSIFTNLSGKESPYLAAAKSRGTWFNTKELLALGRDKIIQEIKDSGLRGRGGAGFPTGLKWSFMPKALPEGSSAYL
VVNADESEPGTCKDRDIIRYDPHKILEGILIAGFAMGVTAAYIYIRGEFYNEYTVLFKALQEAYADGLLGENACGSGYNL
NVFIHRGAGAYICGEESAQIESIEGRKGMPRLKPPFPAAVGLYGSPTTVNNVETIAVVGDILRRGSGWFSSLGIPNNTGT
KVFCISGHVNNPCNVEEELGIPMRELIEKYAGGVIGGWDNLLAVIPGGSSVPMLPKHICDDITMDFDSLRAAKSGLGTAG
LIVMDKSTDLIEAIERISSFYMHESCGQCSPCREGTGWMWRMMRKMLDGTAEESEIDKLLDVASQIEGHTICALADAAVW
PIQGLFRHFRSVVEERLRSSKARRLA
>Mature_426_residues
MRDEDSIFTNLSGKESPYLAAAKSRGTWFNTKELLALGRDKIIQEIKDSGLRGRGGAGFPTGLKWSFMPKALPEGSSAYL
VVNADESEPGTCKDRDIIRYDPHKILEGILIAGFAMGVTAAYIYIRGEFYNEYTVLFKALQEAYADGLLGENACGSGYNL
NVFIHRGAGAYICGEESAQIESIEGRKGMPRLKPPFPAAVGLYGSPTTVNNVETIAVVGDILRRGSGWFSSLGIPNNTGT
KVFCISGHVNNPCNVEEELGIPMRELIEKYAGGVIGGWDNLLAVIPGGSSVPMLPKHICDDITMDFDSLRAAKSGLGTAG
LIVMDKSTDLIEAIERISSFYMHESCGQCSPCREGTGWMWRMMRKMLDGTAEESEIDKLLDVASQIEGHTICALADAAVW
PIQGLFRHFRSVVEERLRSSKARRLA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=418, Percent_Identity=64.1148325358852, Blast_Score=572, Evalue=1e-163,
Organism=Homo sapiens, GI20149568, Length=418, Percent_Identity=64.1148325358852, Blast_Score=572, Evalue=1e-163,
Organism=Escherichia coli, GI1788620, Length=385, Percent_Identity=42.5974025974026, Blast_Score=309, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17531727, Length=426, Percent_Identity=61.9718309859155, Blast_Score=577, Evalue=1e-165,
Organism=Drosophila melanogaster, GI19920794, Length=423, Percent_Identity=63.5933806146572, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI45550452, Length=426, Percent_Identity=59.8591549295775, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24653819, Length=418, Percent_Identity=47.6076555023923, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24653817, Length=418, Percent_Identity=44.2583732057416, Blast_Score=353, Evalue=8e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46397; Mature: 46397

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDEDSIFTNLSGKESPYLAAAKSRGTWFNTKELLALGRDKIIQEIKDSGLRGRGGAGFP
CCCCCCCEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TGLKWSFMPKALPEGSSAYLVVNADESEPGTCKDRDIIRYDPHKILEGILIAGFAMGVTA
CCCEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHE
AYIYIRGEFYNEYTVLFKALQEAYADGLLGENACGSGYNLNVFIHRGAGAYICGEESAQI
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCE
ESIEGRKGMPRLKPPFPAAVGLYGSPTTVNNVETIAVVGDILRRGSGWFSSLGIPNNTGT
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCC
KVFCISGHVNNPCNVEEELGIPMRELIEKYAGGVIGGWDNLLAVIPGGSSVPMLPKHICD
EEEEEECCCCCCCCCHHHCCCCHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCHHHHHH
DITMDFDSLRAAKSGLGTAGLIVMDKSTDLIEAIERISSFYMHESCGQCSPCREGTGWMW
HHHCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RMMRKMLDGTAEESEIDKLLDVASQIEGHTICALADAAVWPIQGLFRHFRSVVEERLRSS
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KARRLA
HHHCCC
>Mature Secondary Structure
MRDEDSIFTNLSGKESPYLAAAKSRGTWFNTKELLALGRDKIIQEIKDSGLRGRGGAGFP
CCCCCCCEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TGLKWSFMPKALPEGSSAYLVVNADESEPGTCKDRDIIRYDPHKILEGILIAGFAMGVTA
CCCEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHE
AYIYIRGEFYNEYTVLFKALQEAYADGLLGENACGSGYNLNVFIHRGAGAYICGEESAQI
EEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCE
ESIEGRKGMPRLKPPFPAAVGLYGSPTTVNNVETIAVVGDILRRGSGWFSSLGIPNNTGT
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCC
KVFCISGHVNNPCNVEEELGIPMRELIEKYAGGVIGGWDNLLAVIPGGSSVPMLPKHICD
EEEEEECCCCCCCCCHHHCCCCHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCHHHHHH
DITMDFDSLRAAKSGLGTAGLIVMDKSTDLIEAIERISSFYMHESCGQCSPCREGTGWMW
HHHCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RMMRKMLDGTAEESEIDKLLDVASQIEGHTICALADAAVWPIQGLFRHFRSVVEERLRSS
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KARRLA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA