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The map label for this gene is pstA [H]

Identifier: 88607119

GI number: 88607119

Start: 362609

End: 363955

Strand: Reverse

Name: pstA [H]

Synonym: APH_0370

Alternate gene names: 88607119

Gene position: 363955-362609 (Counterclockwise)

Preceding gene: 88607365

Following gene: 88607282

Centisome position: 24.74

GC content: 39.12

Gene sequence:

>1347_bases
ATGACTTTTAAAGGTTGTAGCATCTACCTAACTTTGGTAGCACTGTCAGTAAAGGTAGTCTGCAATCTCTCGTTTTTTCA
TTGTATTTTCGGAACATTTATGGGCAAGAATTTGGCGAGGTTGCTTAAGCAACACAGAAAAAGCATGCGTGTATTCTTCA
GAAGCAAACAAGATCGATTTTTTTCTCTACTCTCTCTATCAGCCTTAGGCATTTCTGTAGGAATGTTGATGCTAGTCCTC
TGTAGTATTGTAAATAACGGATACAAAGCCCTGACCGCAACTAAGATCCTTCTACACCTAGATGATACCGAAAGCATAAG
AAAAGACAGTGAATACTTTAGAGAAGACTGCATAAAGCGCATACATAGCGCACTAGATAAAATCCTCAGCAGCCATGTTG
ATTTGAACAACCCTGAAACTAAGAGAATAGCCTATAGTATCGTTAGCGAAACAGCACACTATGAACTTGCAGACTTAATT
CAAAAGAATGTACGTATTAGCCCTGATGGATTGTGGCTAAATGCCTCTACAGAATTCCTAAGTGGCGTTAAAAATAATGA
AATATCTAGCGTGGTGCTCAAAACATTTGAAGATCAAGAACGAATAAAGAGAAGCTTCTTTAATAAATCTCTCTTTACTA
ACTCACATTCCAAAGTACCAGAAAATGCTGGCATATTAGGTGCGCTTATCGGCTCCTTACTGACTATAATAGTGTGTTTA
GCAATTGCCATTCCCCTGGGCATTATGACAGGAATATGTATGCAAGAACTGTTGTCGAATAACAAAGTATCATCAATAAT
CGAGATCAGTATTAATAACCTGTCGTCTGTACCTTCAATAATTTTCGGGGTATTGGGCTTGGTGGTATACCTAAATATAT
TTGGCGTGCCCAGATCCTCATCATTGGCAGGCGGCATGACTTTATCAATCATGATACTTCCGTATCTGATATTGACCACA
AGGCAGGCTTTAGCAGCTGTGCCAGAGTCTGTAAAACATGCAGCGCTTTCCTTAGGAGCCACCAATATGCAAGTAATAAT
GCATCACTCATTACCTATAGCTTTCCCTGCTCTGGTACAGGGTGTTTTAGTGAGTGTTGCACGGATTATTGGGGAATCTT
CCCCTTTAATTATGATAGGAATGGTAGCATTTGTTATTGATCTACCTAAAACTTTCCTTGATCCAGCAAGTGTTCTGCCT
GTGCAAATTTATATTTGGGCAAACAATTCTAGCGCTGAGTTCACAAAGATTTCAGCAATAGCCATACTAGCCTTGCTGAT
GATCTTACTCGTACTAAACATGATAGCCGCATTTATCAAGAGACGCTTAGATAATTTCTCCTTTTAA

Upstream 100 bases:

>100_bases
GATACATGCTTAACACAAAATACTTCAAGAACATCATCAAGGGGCAAATTGTCTGCTTTTATGTGGTTTTTCTACATCGA
GCCTCACAAAACTCTTTCTA

Downstream 100 bases:

>100_bases
GTTATATCGCCTGAATTAAATGGAACACTATTTTTATGAGACTACGCACACCAGCACTACAGCAACATTGATGCCAGAAT
CATCGCCTGCAGCATCCACA

Product: phosphate ABC transporter, permease protein PtsA

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTFKGCSIYLTLVALSVKVVCNLSFFHCIFGTFMGKNLARLLKQHRKSMRVFFRSKQDRFFSLLSLSALGISVGMLMLVL
CSIVNNGYKALTATKILLHLDDTESIRKDSEYFREDCIKRIHSALDKILSSHVDLNNPETKRIAYSIVSETAHYELADLI
QKNVRISPDGLWLNASTEFLSGVKNNEISSVVLKTFEDQERIKRSFFNKSLFTNSHSKVPENAGILGALIGSLLTIIVCL
AIAIPLGIMTGICMQELLSNNKVSSIIEISINNLSSVPSIIFGVLGLVVYLNIFGVPRSSSLAGGMTLSIMILPYLILTT
RQALAAVPESVKHAALSLGATNMQVIMHHSLPIAFPALVQGVLVSVARIIGESSPLIMIGMVAFVIDLPKTFLDPASVLP
VQIYIWANNSSAEFTKISAIAILALLMILLVLNMIAAFIKRRLDNFSF

Sequences:

>Translated_448_residues
MTFKGCSIYLTLVALSVKVVCNLSFFHCIFGTFMGKNLARLLKQHRKSMRVFFRSKQDRFFSLLSLSALGISVGMLMLVL
CSIVNNGYKALTATKILLHLDDTESIRKDSEYFREDCIKRIHSALDKILSSHVDLNNPETKRIAYSIVSETAHYELADLI
QKNVRISPDGLWLNASTEFLSGVKNNEISSVVLKTFEDQERIKRSFFNKSLFTNSHSKVPENAGILGALIGSLLTIIVCL
AIAIPLGIMTGICMQELLSNNKVSSIIEISINNLSSVPSIIFGVLGLVVYLNIFGVPRSSSLAGGMTLSIMILPYLILTT
RQALAAVPESVKHAALSLGATNMQVIMHHSLPIAFPALVQGVLVSVARIIGESSPLIMIGMVAFVIDLPKTFLDPASVLP
VQIYIWANNSSAEFTKISAIAILALLMILLVLNMIAAFIKRRLDNFSF
>Mature_447_residues
TFKGCSIYLTLVALSVKVVCNLSFFHCIFGTFMGKNLARLLKQHRKSMRVFFRSKQDRFFSLLSLSALGISVGMLMLVLC
SIVNNGYKALTATKILLHLDDTESIRKDSEYFREDCIKRIHSALDKILSSHVDLNNPETKRIAYSIVSETAHYELADLIQ
KNVRISPDGLWLNASTEFLSGVKNNEISSVVLKTFEDQERIKRSFFNKSLFTNSHSKVPENAGILGALIGSLLTIIVCLA
IAIPLGIMTGICMQELLSNNKVSSIIEISINNLSSVPSIIFGVLGLVVYLNIFGVPRSSSLAGGMTLSIMILPYLILTTR
QALAAVPESVKHAALSLGATNMQVIMHHSLPIAFPALVQGVLVSVARIIGESSPLIMIGMVAFVIDLPKTFLDPASVLPV
QIYIWANNSSAEFTKISAIAILALLMILLVLNMIAAFIKRRLDNFSF

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=219, Percent_Identity=31.0502283105023, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790164, Length=210, Percent_Identity=24.2857142857143, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 49199; Mature: 49068

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKGCSIYLTLVALSVKVVCNLSFFHCIFGTFMGKNLARLLKQHRKSMRVFFRSKQDRF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLLSLSALGISVGMLMLVLCSIVNNGYKALTATKILLHLDDTESIRKDSEYFREDCIKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHH
IHSALDKILSSHVDLNNPETKRIAYSIVSETAHYELADLIQKNVRISPDGLWLNASTEFL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEECCCHHHH
SGVKNNEISSVVLKTFEDQERIKRSFFNKSLFTNSHSKVPENAGILGALIGSLLTIIVCL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AIAIPLGIMTGICMQELLSNNKVSSIIEISINNLSSVPSIIFGVLGLVVYLNIFGVPRSS
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLAGGMTLSIMILPYLILTTRQALAAVPESVKHAALSLGATNMQVIMHHSLPIAFPALVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHHHH
GVLVSVARIIGESSPLIMIGMVAFVIDLPKTFLDPASVLPVQIYIWANNSSAEFTKISAI
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEEECCCCCHHHHHHHH
AILALLMILLVLNMIAAFIKRRLDNFSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFKGCSIYLTLVALSVKVVCNLSFFHCIFGTFMGKNLARLLKQHRKSMRVFFRSKQDRF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLLSLSALGISVGMLMLVLCSIVNNGYKALTATKILLHLDDTESIRKDSEYFREDCIKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHH
IHSALDKILSSHVDLNNPETKRIAYSIVSETAHYELADLIQKNVRISPDGLWLNASTEFL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEECCCHHHH
SGVKNNEISSVVLKTFEDQERIKRSFFNKSLFTNSHSKVPENAGILGALIGSLLTIIVCL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AIAIPLGIMTGICMQELLSNNKVSSIIEISINNLSSVPSIIFGVLGLVVYLNIFGVPRSS
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLAGGMTLSIMILPYLILTTRQALAAVPESVKHAALSLGATNMQVIMHHSLPIAFPALVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHHHH
GVLVSVARIIGESSPLIMIGMVAFVIDLPKTFLDPASVLPVQIYIWANNSSAEFTKISAI
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEEECCCCCHHHHHHHH
AILALLMILLVLNMIAAFIKRRLDNFSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]