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The map label for this gene is folK [H]

Identifier: 88607066

GI number: 88607066

Start: 208442

End: 208972

Strand: Reverse

Name: folK [H]

Synonym: APH_0212

Alternate gene names: 88607066

Gene position: 208972-208442 (Counterclockwise)

Preceding gene: 88606986

Following gene: 88607777

Centisome position: 14.2

GC content: 38.42

Gene sequence:

>531_bases
ATGGTTAAACAAATATATCAACCCAACAAAAGTGCTATGGTTGTATTAGCATTGGGCAGCAATATTGGCGATAGGATGAG
CGCTTTAAAAGAAGCAATAAAACTCATACCGTTGCATAATAGACTATACTCATCCGTATATGAGAGTTGTGCTTTGCTTC
CTGATAACGCCCCATTTTGCTGGAACATGCCTTTTCTAAACATGGTCATCGCGGGATATACACATTTTTCTCCAGAAGAT
CTCTTACAAAGCATTAAACAAATAGAATCCCAGCTAGGAAGATGTAGTCTTGGTCATTGGAGCCCTAGAAGCATAGATAT
AGACATAATATTGTGGGAAGGCGTTACAGTAGAGGATAAAGCTCTCACTATACCTCATAAGGAAATGCACAAACGTGACT
TTGTTCTAGTACCTACCTGCGACATATGTCCCCGTTTTCCACATCCTATACTAAAAGAAACAGTAGAATCGATATTGCTA
AATAAGCAGGATATCAACCTTGTGAAAAAGTATCAGGCTATACACTTATAG

Upstream 100 bases:

>100_bases
TTATTAACAAATTTTATTATCCTACAAAATCCAGTGCAGTTGCCTATTGCTTCAGAGTATGCTAGGTGCATGGCAGTGGA
TTTTTAGATCTTTTCTCAAA

Downstream 100 bases:

>100_bases
TGAGGGAGAATGGCCTCTATATGATTCATTAAAACACCAGTGCAAGACTCCATTCCGAGGCACAGTGCTGATTATTATCT
GTAAAACTACGCTTCCCATT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MVKQIYQPNKSAMVVLALGSNIGDRMSALKEAIKLIPLHNRLYSSVYESCALLPDNAPFCWNMPFLNMVIAGYTHFSPED
LLQSIKQIESQLGRCSLGHWSPRSIDIDIILWEGVTVEDKALTIPHKEMHKRDFVLVPTCDICPRFPHPILKETVESILL
NKQDINLVKKYQAIHL

Sequences:

>Translated_176_residues
MVKQIYQPNKSAMVVLALGSNIGDRMSALKEAIKLIPLHNRLYSSVYESCALLPDNAPFCWNMPFLNMVIAGYTHFSPED
LLQSIKQIESQLGRCSLGHWSPRSIDIDIILWEGVTVEDKALTIPHKEMHKRDFVLVPTCDICPRFPHPILKETVESILL
NKQDINLVKKYQAIHL
>Mature_176_residues
MVKQIYQPNKSAMVVLALGSNIGDRMSALKEAIKLIPLHNRLYSSVYESCALLPDNAPFCWNMPFLNMVIAGYTHFSPED
LLQSIKQIESQLGRCSLGHWSPRSIDIDIILWEGVTVEDKALTIPHKEMHKRDFVLVPTCDICPRFPHPILKETVESILL
NKQDINLVKKYQAIHL

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=33.8129496402878, Blast_Score=77, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324073, Length=151, Percent_Identity=35.0993377483444, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 20050; Mature: 20050

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKQIYQPNKSAMVVLALGSNIGDRMSALKEAIKLIPLHNRLYSSVYESCALLPDNAPFC
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCE
WNMPFLNMVIAGYTHFSPEDLLQSIKQIESQLGRCSLGHWSPRSIDIDIILWEGVTVEDK
ECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEECCC
ALTIPHKEMHKRDFVLVPTCDICPRFPHPILKETVESILLNKQDINLVKKYQAIHL
EEECCHHHHHCCCEEEEECHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKQIYQPNKSAMVVLALGSNIGDRMSALKEAIKLIPLHNRLYSSVYESCALLPDNAPFC
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCE
WNMPFLNMVIAGYTHFSPEDLLQSIKQIESQLGRCSLGHWSPRSIDIDIILWEGVTVEDK
ECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEECCC
ALTIPHKEMHKRDFVLVPTCDICPRFPHPILKETVESILLNKQDINLVKKYQAIHL
EEECCHHHHHCCCEEEEECHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]