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The map label for this gene is purN [H]

Identifier: 88606915

GI number: 88606915

Start: 94007

End: 94477

Strand: Reverse

Name: purN [H]

Synonym: APH_0087

Alternate gene names: 88606915

Gene position: 94477-94007 (Counterclockwise)

Preceding gene: 88607628

Following gene: 88607297

Centisome position: 6.42

GC content: 44.16

Gene sequence:

>471_bases
TTGACCTTTGTTGTTAGGCGGAAACCGCTGGATATAGAGCACATCAGTACAGTACTAAGGGAGCACGATGTAGGTCTAGT
GTGCCTTGCAGGATTCATGAGCATACTGCCTGAAAAGTTTGTCACAGATTGGCATCACAAGATAATTAACATTCACCCTT
CACTTCTCCCATCTTTTAAGGGATTAAATGCTCAGGAACAAGCCTATAAAGCAGGTGTTAAAATAGCGGGGTGCACCCTA
CATTATGTATATCAAGAGCTTGATGCCGGTCCTATAATAATGCAGGCTGCAGTGCCTGTGCTTAGAGAAGACACTGCTGA
GAGTTTAGCTAGTCGTATTCTAGCTGCTGAGCACGTATGTTATCCCAAGGGTGTGAAGCTCATTGCTCAGGATAAAATCA
AGCTTTGCGATGATGGCACTGTGCAATGTACGGGCGAGGATGAGCTCTTCTTATTTCAGGAGAATTTCTAA

Upstream 100 bases:

>100_bases
CCTAACTATCACCCCCCATTTCAGCAATCACAGTAGCTAGAGTATCTGTTAGTACTGAACATGGTTTTTAGGAGAGCTTA
TAGAATCTCACGCAGATCTC

Downstream 100 bases:

>100_bases
CAAAAAGTTGTGAAGGAACTCTTGCTCACCTTCTATGAACAAAAATCACACTATAAGTCGTCACATAGAATAGAAGGCAG
TCAGCCTAGCTCATATAAGG

Product: putative phosphoribosylglycinamide formyltransferase, truncation

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTFVVRRKPLDIEHISTVLREHDVGLVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQEQAYKAGVKIAGCTL
HYVYQELDAGPIIMQAAVPVLREDTAESLASRILAAEHVCYPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF

Sequences:

>Translated_156_residues
MTFVVRRKPLDIEHISTVLREHDVGLVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQEQAYKAGVKIAGCTL
HYVYQELDAGPIIMQAAVPVLREDTAESLASRILAAEHVCYPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF
>Mature_155_residues
TFVVRRKPLDIEHISTVLREHDVGLVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFKGLNAQEQAYKAGVKIAGCTLH
YVYQELDAGPIIMQAAVPVLREDTAESLASRILAAEHVCYPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=127, Percent_Identity=50.3937007874016, Blast_Score=140, Evalue=6e-34,
Organism=Homo sapiens, GI209869995, Length=127, Percent_Identity=50.3937007874016, Blast_Score=140, Evalue=6e-34,
Organism=Homo sapiens, GI209869993, Length=127, Percent_Identity=50.3937007874016, Blast_Score=140, Evalue=6e-34,
Organism=Escherichia coli, GI1788846, Length=113, Percent_Identity=39.8230088495575, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1787483, Length=83, Percent_Identity=50.6024096385542, Blast_Score=81, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=115, Percent_Identity=42.6086956521739, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24582400, Length=122, Percent_Identity=45.9016393442623, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 17432; Mature: 17301

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFVVRRKPLDIEHISTVLREHDVGLVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFK
CEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCC
GLNAQEQAYKAGVKIAGCTLHYVYQELDAGPIIMQAAVPVLREDTAESLASRILAAEHVC
CCCHHHHHHHCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF
CCCCCEEEECCCEEECCCCEEEECCCCCEEEEECCC
>Mature Secondary Structure 
TFVVRRKPLDIEHISTVLREHDVGLVCLAGFMSILPEKFVTDWHHKIINIHPSLLPSFK
EEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCC
GLNAQEQAYKAGVKIAGCTLHYVYQELDAGPIIMQAAVPVLREDTAESLASRILAAEHVC
CCCHHHHHHHCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YPKGVKLIAQDKIKLCDDGTVQCTGEDELFLFQENF
CCCCCEEEECCCEEECCCCEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]