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The map label for this gene is def [H]

Identifier: 88606874

GI number: 88606874

Start: 1070312

End: 1070905

Strand: Reverse

Name: def [H]

Synonym: APH_1012

Alternate gene names: 88606874

Gene position: 1070905-1070312 (Counterclockwise)

Preceding gene: 88606824

Following gene: 88607596

Centisome position: 72.79

GC content: 41.25

Gene sequence:

>594_bases
ATGAGGGTTCTATCTGTAGAGGATCAACGGGATTTGAAGGTATTACATACCGTATCATCTCCTTTGGAAAGCGTTGATAG
CGCAACTCGAGAAATTGTAAATGAGATGATCAAAATTGCTGAGAGGGGAGACACTGTGGGACTATCTGCAGTGCAATTGG
GGTACCCCATTAGAGTCTTTGTTATAGATATGTTCAGTGGCCTTTTTAACATTACTGAAGATCTGAAGGTGATTTCAGGG
CATCATTCACACAATACCAGAAGTCTCGTATGCATAAATCCCCAAATTGTGAGCTTCTCTGGCGAAACTGTTACTCTGTT
TGAAGGGTGTTTATCTGTGAAATCTTACGGGATGGTGGGAATACAGCGTCCTGGAAATGTTGATCTGAAATATACTGATC
TTGCGGGGAACGTGTGCGTGATAAGAACATTCAATTGGCTTGCTAGATGTGTGCAGCATGAGATGGATCACCTTAACGGA
GTGTTATTGGCTAATATGCTGGATAACATAAAAAATAAATCTGCCCAGAGTGTTTCAGAAGAAGACTTTAGCTCAGTGCA
TATACTGCTTTTAGACAAAAACAAACAGGGCTAG

Upstream 100 bases:

>100_bases
AACATAAGAGATATGTTTTGTTTAGCATATAACAAAGGCCCCTAGGGTTTATTGTTTGTAGCTTCATAAATCTTTTACTG
GTGAAACAAGAGGAGAGAAC

Downstream 100 bases:

>100_bases
GCGTTGGGGTATCGGAGGGATCTTTGAAAATCACAACGATTAGGGATTTTCTTCCAGATTTTGATTAGTTAACTTTGGGC
TTCTGCATTTATTACCGAGA

Product: putative polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MRVLSVEDQRDLKVLHTVSSPLESVDSATREIVNEMIKIAERGDTVGLSAVQLGYPIRVFVIDMFSGLFNITEDLKVISG
HHSHNTRSLVCINPQIVSFSGETVTLFEGCLSVKSYGMVGIQRPGNVDLKYTDLAGNVCVIRTFNWLARCVQHEMDHLNG
VLLANMLDNIKNKSAQSVSEEDFSSVHILLLDKNKQG

Sequences:

>Translated_197_residues
MRVLSVEDQRDLKVLHTVSSPLESVDSATREIVNEMIKIAERGDTVGLSAVQLGYPIRVFVIDMFSGLFNITEDLKVISG
HHSHNTRSLVCINPQIVSFSGETVTLFEGCLSVKSYGMVGIQRPGNVDLKYTDLAGNVCVIRTFNWLARCVQHEMDHLNG
VLLANMLDNIKNKSAQSVSEEDFSSVHILLLDKNKQG
>Mature_197_residues
MRVLSVEDQRDLKVLHTVSSPLESVDSATREIVNEMIKIAERGDTVGLSAVQLGYPIRVFVIDMFSGLFNITEDLKVISG
HHSHNTRSLVCINPQIVSFSGETVTLFEGCLSVKSYGMVGIQRPGNVDLKYTDLAGNVCVIRTFNWLARCVQHEMDHLNG
VLLANMLDNIKNKSAQSVSEEDFSSVHILLLDKNKQG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=173, Percent_Identity=30.0578034682081, Blast_Score=77, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24645728, Length=151, Percent_Identity=31.1258278145695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24645726, Length=150, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21830; Mature: 21830

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLSVEDQRDLKVLHTVSSPLESVDSATREIVNEMIKIAERGDTVGLSAVQLGYPIRVF
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHH
VIDMFSGLFNITEDLKVISGHHSHNTRSLVCINPQIVSFSGETVTLFEGCLSVKSYGMVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCEEHHHHHHHHHHHCCEEE
IQRPGNVDLKYTDLAGNVCVIRTFNWLARCVQHEMDHLNGVLLANMLDNIKNKSAQSVSE
ECCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
EDFSSVHILLLDKNKQG
CCCCEEEEEEEECCCCC
>Mature Secondary Structure
MRVLSVEDQRDLKVLHTVSSPLESVDSATREIVNEMIKIAERGDTVGLSAVQLGYPIRVF
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHH
VIDMFSGLFNITEDLKVISGHHSHNTRSLVCINPQIVSFSGETVTLFEGCLSVKSYGMVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCEEHHHHHHHHHHHCCEEE
IQRPGNVDLKYTDLAGNVCVIRTFNWLARCVQHEMDHLNGVLLANMLDNIKNKSAQSVSE
ECCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
EDFSSVHILLLDKNKQG
CCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA