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The map label for this gene is tgt [H]

Identifier: 88606822

GI number: 88606822

Start: 299582

End: 300766

Strand: Reverse

Name: tgt [H]

Synonym: APH_0314

Alternate gene names: 88606822

Gene position: 300766-299582 (Counterclockwise)

Preceding gene: 88607031

Following gene: 88607897

Centisome position: 20.44

GC content: 42.78

Gene sequence:

>1185_bases
GTGTTTATGTATAGAAATTTTAACTTTAAAGTTCTGTTAGAATCCGGAAAAGCAAGAGTTGGTACAATCACAACTCCTCA
CGGGTCTATTTTTACTCCAGCATTCATTTTCTGCGCTACCAAGGCCTCCATAAAGTCTCTAAAAATACAAGATGTGCAAG
AATGCGGCACTCAAATCATTCTATCTAACACTTACCATCTTATGCTGCAACCCGGAGAGCAAGTAGTTGAATCACTAGGT
GGCTTGCACAAAATGATGGGCTGGAACGGCCCCATGCTTACAGACTCAGGTGGATATCAAATCTTCAGCCTAGGTTATGG
TTCTGTCTCCGAAGAACTTAAAGGGAAGTTACGCAAATCATCAGGTACGTCCCTGATAAAAATTGATGAAAGTGGCGCGC
GCTTTAGATCATATATAAACGGTAGTACCTACCACCTCACCCCCGAAAGATCTGTAGAAATACAGCGCAAATTAGGAGCA
GATTTGGTGGTAGTGCTGGATGAGTGCACTCCATTCCACACAAGCTATGAATACACAAATCAGTCCATGCTTATGAGCAC
CAGGTGGGCACAGAGATCTCTAGAAGCCTTTAAAAGCACAGACGATGGATCACAAGCCCTATACTGCGTGATACAAGGAG
GTGTGTATCCTGACCTACGTAAGACTAGCTGTGAGTTCGCAAATAATTCTCCTTTCTTCGGACATGCAATAGGAGGATCT
CTTGGTGCCTGTAAGCAACAGATGTACGATATAGTGTCTCTAACTTACAATATGCTCGATAAAACTCGTCCAGTACACCT
CTTGGGCATCGGGAACGTAATAGATATATTTCACGGAGTCCTACAGGGTATCGATACTTTCGACTGCGTGTATCCTACAA
GAATTGCACGCCATGGAGCTGCTCTCGTAAAACCCGAGAACAGAGATATGCATGGAAGAGAATACATAAACCTGAATTCC
GCAAAATTCAGAACCAGTACAGATGCAATAGAGCAAGATTGCATCTGCTATACATGCAGAACACATTCAAAAGGATATCT
ACACCACCTACTGAAGGCAAAGGAGCTACTAGCCTATTCATTAATAACCATACACAACATACAGTTCATGAACGACCTGA
TGCAACTCATTAGAAAGTCAATCAGCAACAATAGCCTACAACAAGAACTGACTCGATGGAGCTAG

Upstream 100 bases:

>100_bases
GCATTCATGAAACAGTGTACTCTGCTGTTATAGCTAATTTTTGATGCAAAATATGCATTAATACCCTACTATTATCCACA
CTTTTCCTACCACCTGTAAC

Downstream 100 bases:

>100_bases
TATGCAACCATCACGAAAGTCCACGGTTACATTTTGGATCCACCATAGCGCTATGTGAAATAAAGAATGCTAAATCCTAT
GGATGTTCCTCAAGATAACC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MFMYRNFNFKVLLESGKARVGTITTPHGSIFTPAFIFCATKASIKSLKIQDVQECGTQIILSNTYHLMLQPGEQVVESLG
GLHKMMGWNGPMLTDSGGYQIFSLGYGSVSEELKGKLRKSSGTSLIKIDESGARFRSYINGSTYHLTPERSVEIQRKLGA
DLVVVLDECTPFHTSYEYTNQSMLMSTRWAQRSLEAFKSTDDGSQALYCVIQGGVYPDLRKTSCEFANNSPFFGHAIGGS
LGACKQQMYDIVSLTYNMLDKTRPVHLLGIGNVIDIFHGVLQGIDTFDCVYPTRIARHGAALVKPENRDMHGREYINLNS
AKFRTSTDAIEQDCICYTCRTHSKGYLHHLLKAKELLAYSLITIHNIQFMNDLMQLIRKSISNNSLQQELTRWS

Sequences:

>Translated_394_residues
MFMYRNFNFKVLLESGKARVGTITTPHGSIFTPAFIFCATKASIKSLKIQDVQECGTQIILSNTYHLMLQPGEQVVESLG
GLHKMMGWNGPMLTDSGGYQIFSLGYGSVSEELKGKLRKSSGTSLIKIDESGARFRSYINGSTYHLTPERSVEIQRKLGA
DLVVVLDECTPFHTSYEYTNQSMLMSTRWAQRSLEAFKSTDDGSQALYCVIQGGVYPDLRKTSCEFANNSPFFGHAIGGS
LGACKQQMYDIVSLTYNMLDKTRPVHLLGIGNVIDIFHGVLQGIDTFDCVYPTRIARHGAALVKPENRDMHGREYINLNS
AKFRTSTDAIEQDCICYTCRTHSKGYLHHLLKAKELLAYSLITIHNIQFMNDLMQLIRKSISNNSLQQELTRWS
>Mature_394_residues
MFMYRNFNFKVLLESGKARVGTITTPHGSIFTPAFIFCATKASIKSLKIQDVQECGTQIILSNTYHLMLQPGEQVVESLG
GLHKMMGWNGPMLTDSGGYQIFSLGYGSVSEELKGKLRKSSGTSLIKIDESGARFRSYINGSTYHLTPERSVEIQRKLGA
DLVVVLDECTPFHTSYEYTNQSMLMSTRWAQRSLEAFKSTDDGSQALYCVIQGGVYPDLRKTSCEFANNSPFFGHAIGGS
LGACKQQMYDIVSLTYNMLDKTRPVHLLGIGNVIDIFHGVLQGIDTFDCVYPTRIARHGAALVKPENRDMHGREYINLNS
AKFRTSTDAIEQDCICYTCRTHSKGYLHHLLKAKELLAYSLITIHNIQFMNDLMQLIRKSISNNSLQQELTRWS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=366, Percent_Identity=36.6120218579235, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1786607, Length=384, Percent_Identity=39.0625, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17544680, Length=388, Percent_Identity=33.5051546391753, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI25146502, Length=141, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19920510, Length=385, Percent_Identity=35.5844155844156, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI21355311, Length=261, Percent_Identity=25.6704980842912, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 44212; Mature: 44212

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMYRNFNFKVLLESGKARVGTITTPHGSIFTPAFIFCATKASIKSLKIQDVQECGTQII
CEEEECCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHCCHHHHHHCCHHHHHHCCCEEE
LSNTYHLMLQPGEQVVESLGGLHKMMGWNGPMLTDSGGYQIFSLGYGSVSEELKGKLRKS
EECCEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHC
SGTSLIKIDESGARFRSYINGSTYHLTPERSVEIQRKLGADLVVVLDECTPFHTSYEYTN
CCCEEEEECCCCHHHHHHCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHCC
QSMLMSTRWAQRSLEAFKSTDDGSQALYCVIQGGVYPDLRKTSCEFANNSPFFGHAIGGS
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCEEECCCCC
LGACKQQMYDIVSLTYNMLDKTRPVHLLGIGNVIDIFHGVLQGIDTFDCVYPTRIARHGA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHCHHHHHHCCC
ALVKPENRDMHGREYINLNSAKFRTSTDAIEQDCICYTCRTHSKGYLHHLLKAKELLAYS
EEECCCCCCCCCCEEEECCCCEEECCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
LITIHNIQFMNDLMQLIRKSISNNSLQQELTRWS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MFMYRNFNFKVLLESGKARVGTITTPHGSIFTPAFIFCATKASIKSLKIQDVQECGTQII
CEEEECCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHCCHHHHHHCCHHHHHHCCCEEE
LSNTYHLMLQPGEQVVESLGGLHKMMGWNGPMLTDSGGYQIFSLGYGSVSEELKGKLRKS
EECCEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHC
SGTSLIKIDESGARFRSYINGSTYHLTPERSVEIQRKLGADLVVVLDECTPFHTSYEYTN
CCCEEEEECCCCHHHHHHCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHCC
QSMLMSTRWAQRSLEAFKSTDDGSQALYCVIQGGVYPDLRKTSCEFANNSPFFGHAIGGS
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCEEECCCCC
LGACKQQMYDIVSLTYNMLDKTRPVHLLGIGNVIDIFHGVLQGIDTFDCVYPTRIARHGA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHCHHHHHHCCC
ALVKPENRDMHGREYINLNSAKFRTSTDAIEQDCICYTCRTHSKGYLHHLLKAKELLAYS
EEECCCCCCCCCCEEEECCCCEEECCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
LITIHNIQFMNDLMQLIRKSISNNSLQQELTRWS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA