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The map label for this gene is rne [H]

Identifier: 88606792

GI number: 88606792

Start: 328494

End: 330404

Strand: Direct

Name: rne [H]

Synonym: APH_0345

Alternate gene names: 88606792

Gene position: 328494-330404 (Clockwise)

Preceding gene: 88607394

Following gene: 88607549

Centisome position: 22.33

GC content: 42.96

Gene sequence:

>1911_bases
TTGTGTTTTGTGTCTAATAGTGGTAGTGGAAAGAGAATATTAATTGATGCCGTATACCTAGATGAGATAAGAGTTGCGGT
GCTCGACGAGGGAAAGGTTGTTGAGTTTGATCAGGAATTAAAGGATAGGAAGCAGTTACGTGGGGGGAATATTTATTTCG
CTGTTGTTCGGCGTATTGAGCCTTCATTGCAGGCTGTTTTTATAGAGTATGGTGTAGGAAAGCACGGGTTTTTGCCGTTT
TCAGAAATAGCGGTTGAGCATTATCAAATTCCTCAGGAAGAGAAAGATGTTCTGTTGAAGAATCTGTATGGTGATAATTC
GGAGCAAGATGAGCACTCTGAAGAGGAAGAGTGTACGGGAGATGCAGAGCCTTCTGAAGAAAGTAGTTCAGAAGATGCTG
ATAAAGTGCAGTCCTTAGCTGAGGAACAGCGAGCAGGCAAGAGGTGTTCTGAAGGCAAGAATGGCAAGGGCAAGTTGGTG
CCGCACAAGATGTATAAGGTGCAGAATGTCATAAAGGTTGATCAGAAACTCATGGTTCAGATCACGAAGGAGGAGAGGGG
TAGCAAGTGCGCTACTTTTTCGACTTATATAGTTCTTGCAGGAAGGTATTGCATTTTTATGCCAAATTCTGGAAACAAGA
ACAGTGGCATATCGCGGCGTATAGATGATTGTGTCCACAGGAAAAATTTGAAAGAGTTTCTAGATGAGATGAAGCTTCCA
AAAGAGGCTGGTGTTATTATTAGGACTGCTGGGAGCAATAGAACTCATAAGGAATTGGAGCGAGATTTATCGTACTTACA
GTCTACGTGGGATGAGGTAAAAAGTAGTTTTGAGTCTGTTACGCATCCTTCTCTATTGTACGTAGAAGCTGATGTGATAA
AGCGCACTTTGCGCGATTTCTGTGATGAGAACGTCCATGATGTTGTGGTAGCTGGTAGTAAGGCATTTGGTCTTGTGAAG
GAGTACGCAAAGTCTATGTTCGGCAATAGGCTGCGTGTAAGGACATATAAGGGTAGTATTCCCATATTTACGCATTATAG
GGTCAATGCTCAGATTTTGGAATTGTATACAGATAAGATATATCTGCCTTCTGGAGGGTATTTGGTAATAACACCTACGG
AGGCTTTAGTTTCCATAGACGTGAATTCTGGAAGAATGACAGGAGAAGATAGCATCGAAGAAACCGCCTATAGGACGAAT
ATGGAGGCCGTAAAAGAGATTGCTAGGCAGGTGAATTTACGGGGATTATCCGGGCTCATTGTGATAGATTTCATAGATAT
GGTAAAGTATCGCTATTGCAGGGCTGTTGAGGCTGAGGTGCGCGAAGCTTTCAAGGGGGATAAAGCTAAGATTCAGTTTG
GGTACATAAGTGCATTTGGTTTGATGGAAATTTCCAGGCAGAGGGTGAAGCAGAGTATCCTGGATAGCAGCACAGTGCAG
TGTCATCATTGCAAGGGGGTTGGTAGAACGAGGTCATTAGAGTCTATTTCTGCTAGTATTTTGCGTGATGTTAGTTACGT
GGCTAACAAGAATCCTGAAAAGATACTTGTGGTTTCTGTTGTTTCTTCGGTTGTATCGCATCTATTCAATAGCAAGCGAA
GGCAGATTGCTGAGATAGAGAGTGAATTCAAAGTTCTCATCAGGCTTGATGTAGATGATAATCTAGGTATATCTGATTAT
AATATTCGTGCTGAGAAGGGCTATAGTAGCTTGCTTCATGAGGAGTTGTCTAGTGCTGTGGTCGTGACGGCAGATTCTGT
TAAGAAAAAAGAGGAAGCAGCGGAATTGCCTGCTGAGGGTACTGATCATCAGAAGAGTCTGGCAGAAAATGTTGCGGAGG
TTGGAGAGAGCGTGGTTGCGGTGACTTGGGTTGATAAGTGGTTAACGCGGATGCTCTCTAAAATATCGTAA

Upstream 100 bases:

>100_bases
GTCTTGAGCTAGGTCAAGTATTGCCATGTGTGCATATCTAGCTTCTCGTGGGTATTGGGTTACGTGGTGTAAGGTAGTTT
TTTAATTTATTTAGTGTTGG

Downstream 100 bases:

>100_bases
GCATAGTTAGTGGGGTATTTGAGGCTTTTGGTGCTTCAATATAGAGCTAGTAACGGGGCGTGATGTTTGTTGGTAGCGTT
TGTATTACTAGTTTCCTGAC

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MCFVSNSGSGKRILIDAVYLDEIRVAVLDEGKVVEFDQELKDRKQLRGGNIYFAVVRRIEPSLQAVFIEYGVGKHGFLPF
SEIAVEHYQIPQEEKDVLLKNLYGDNSEQDEHSEEEECTGDAEPSEESSSEDADKVQSLAEEQRAGKRCSEGKNGKGKLV
PHKMYKVQNVIKVDQKLMVQITKEERGSKCATFSTYIVLAGRYCIFMPNSGNKNSGISRRIDDCVHRKNLKEFLDEMKLP
KEAGVIIRTAGSNRTHKELERDLSYLQSTWDEVKSSFESVTHPSLLYVEADVIKRTLRDFCDENVHDVVVAGSKAFGLVK
EYAKSMFGNRLRVRTYKGSIPIFTHYRVNAQILELYTDKIYLPSGGYLVITPTEALVSIDVNSGRMTGEDSIEETAYRTN
MEAVKEIARQVNLRGLSGLIVIDFIDMVKYRYCRAVEAEVREAFKGDKAKIQFGYISAFGLMEISRQRVKQSILDSSTVQ
CHHCKGVGRTRSLESISASILRDVSYVANKNPEKILVVSVVSSVVSHLFNSKRRQIAEIESEFKVLIRLDVDDNLGISDY
NIRAEKGYSSLLHEELSSAVVVTADSVKKKEEAAELPAEGTDHQKSLAENVAEVGESVVAVTWVDKWLTRMLSKIS

Sequences:

>Translated_636_residues
MCFVSNSGSGKRILIDAVYLDEIRVAVLDEGKVVEFDQELKDRKQLRGGNIYFAVVRRIEPSLQAVFIEYGVGKHGFLPF
SEIAVEHYQIPQEEKDVLLKNLYGDNSEQDEHSEEEECTGDAEPSEESSSEDADKVQSLAEEQRAGKRCSEGKNGKGKLV
PHKMYKVQNVIKVDQKLMVQITKEERGSKCATFSTYIVLAGRYCIFMPNSGNKNSGISRRIDDCVHRKNLKEFLDEMKLP
KEAGVIIRTAGSNRTHKELERDLSYLQSTWDEVKSSFESVTHPSLLYVEADVIKRTLRDFCDENVHDVVVAGSKAFGLVK
EYAKSMFGNRLRVRTYKGSIPIFTHYRVNAQILELYTDKIYLPSGGYLVITPTEALVSIDVNSGRMTGEDSIEETAYRTN
MEAVKEIARQVNLRGLSGLIVIDFIDMVKYRYCRAVEAEVREAFKGDKAKIQFGYISAFGLMEISRQRVKQSILDSSTVQ
CHHCKGVGRTRSLESISASILRDVSYVANKNPEKILVVSVVSSVVSHLFNSKRRQIAEIESEFKVLIRLDVDDNLGISDY
NIRAEKGYSSLLHEELSSAVVVTADSVKKKEEAAELPAEGTDHQKSLAENVAEVGESVVAVTWVDKWLTRMLSKIS
>Mature_636_residues
MCFVSNSGSGKRILIDAVYLDEIRVAVLDEGKVVEFDQELKDRKQLRGGNIYFAVVRRIEPSLQAVFIEYGVGKHGFLPF
SEIAVEHYQIPQEEKDVLLKNLYGDNSEQDEHSEEEECTGDAEPSEESSSEDADKVQSLAEEQRAGKRCSEGKNGKGKLV
PHKMYKVQNVIKVDQKLMVQITKEERGSKCATFSTYIVLAGRYCIFMPNSGNKNSGISRRIDDCVHRKNLKEFLDEMKLP
KEAGVIIRTAGSNRTHKELERDLSYLQSTWDEVKSSFESVTHPSLLYVEADVIKRTLRDFCDENVHDVVVAGSKAFGLVK
EYAKSMFGNRLRVRTYKGSIPIFTHYRVNAQILELYTDKIYLPSGGYLVITPTEALVSIDVNSGRMTGEDSIEETAYRTN
MEAVKEIARQVNLRGLSGLIVIDFIDMVKYRYCRAVEAEVREAFKGDKAKIQFGYISAFGLMEISRQRVKQSILDSSTVQ
CHHCKGVGRTRSLESISASILRDVSYVANKNPEKILVVSVVSSVVSHLFNSKRRQIAEIESEFKVLIRLDVDDNLGISDY
NIRAEKGYSSLLHEELSSAVVVTADSVKKKEEAAELPAEGTDHQKSLAENVAEVGESVVAVTWVDKWLTRMLSKIS

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=430, Percent_Identity=40, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI87082235, Length=375, Percent_Identity=32, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 71498; Mature: 71498

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFVSNSGSGKRILIDAVYLDEIRVAVLDEGKVVEFDQELKDRKQLRGGNIYFAVVRRIE
CEEECCCCCCCEEEEEEEEECCEEEEEECCCCEEEHHHHHHHHHHCCCCCEEEEEHHHHC
PSLQAVFIEYGVGKHGFLPFSEIAVEHYQIPQEEKDVLLKNLYGDNSEQDEHSEEEECTG
CCHHEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCC
DAEPSEESSSEDADKVQSLAEEQRAGKRCSEGKNGKGKLVPHKMYKVQNVIKVDQKLMVQ
CCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHE
ITKEERGSKCATFSTYIVLAGRYCIFMPNSGNKNSGISRRIDDCVHRKNLKEFLDEMKLP
EHHHHCCCCCHHHHHHHEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KEAGVIIRTAGSNRTHKELERDLSYLQSTWDEVKSSFESVTHPSLLYVEADVIKRTLRDF
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
CDENVHDVVVAGSKAFGLVKEYAKSMFGNRLRVRTYKGSIPIFTHYRVNAQILELYTDKI
HCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHEEEECCEE
YLPSGGYLVITPTEALVSIDVNSGRMTGEDSIEETAYRTNMEAVKEIARQVNLRGLSGLI
EECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCH
VIDFIDMVKYRYCRAVEAEVREAFKGDKAKIQFGYISAFGLMEISRQRVKQSILDSSTVQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
CHHCKGVGRTRSLESISASILRDVSYVANKNPEKILVVSVVSSVVSHLFNSKRRQIAEIE
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
SEFKVLIRLDVDDNLGISDYNIRAEKGYSSLLHEELSSAVVVTADSVKKKEEAAELPAEG
HCEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCC
TDHQKSLAENVAEVGESVVAVTWVDKWLTRMLSKIS
CCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCFVSNSGSGKRILIDAVYLDEIRVAVLDEGKVVEFDQELKDRKQLRGGNIYFAVVRRIE
CEEECCCCCCCEEEEEEEEECCEEEEEECCCCEEEHHHHHHHHHHCCCCCEEEEEHHHHC
PSLQAVFIEYGVGKHGFLPFSEIAVEHYQIPQEEKDVLLKNLYGDNSEQDEHSEEEECTG
CCHHEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCC
DAEPSEESSSEDADKVQSLAEEQRAGKRCSEGKNGKGKLVPHKMYKVQNVIKVDQKLMVQ
CCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHE
ITKEERGSKCATFSTYIVLAGRYCIFMPNSGNKNSGISRRIDDCVHRKNLKEFLDEMKLP
EHHHHCCCCCHHHHHHHEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KEAGVIIRTAGSNRTHKELERDLSYLQSTWDEVKSSFESVTHPSLLYVEADVIKRTLRDF
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
CDENVHDVVVAGSKAFGLVKEYAKSMFGNRLRVRTYKGSIPIFTHYRVNAQILELYTDKI
HCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHEEEECCEE
YLPSGGYLVITPTEALVSIDVNSGRMTGEDSIEETAYRTNMEAVKEIARQVNLRGLSGLI
EECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCH
VIDFIDMVKYRYCRAVEAEVREAFKGDKAKIQFGYISAFGLMEISRQRVKQSILDSSTVQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
CHHCKGVGRTRSLESISASILRDVSYVANKNPEKILVVSVVSSVVSHLFNSKRRQIAEIE
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
SEFKVLIRLDVDDNLGISDYNIRAEKGYSSLLHEELSSAVVVTADSVKKKEEAAELPAEG
HCEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCC
TDHQKSLAENVAEVGESVVAVTWVDKWLTRMLSKIS
CCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]