Click here to switch to the map view.

The map label for this gene is glyS [H]

Identifier: 88606787

GI number: 88606787

Start: 13256

End: 15361

Strand: Direct

Name: glyS [H]

Synonym: APH_0014

Alternate gene names: 88606787

Gene position: 13256-15361 (Clockwise)

Preceding gene: 88607262

Following gene: 88606746

Centisome position: 0.9

GC content: 38.08

Gene sequence:

>2106_bases
ATGTCTCTTGATTTAGTTTTTGAAATATTATGCGAACAGATGCCATCTAAAATGATGGATATTGCATTGGGGTATCTGAA
AAATACCATCTGTTCTGGGTTTAGTAAGTTTGCTATAGAGAGTCGTAACTCTAGAACTTTAGTCTCGGCAAATCGCATAT
GTTTCATTGCTGAAGGATTGGAACAAGGGAATTCAACTGAAAATAAGGAAATTAAAGGACCGCGAGTATCGTCTTCTCAG
GAATCTATAGCAGGATTCTTGAGAAAGGTTGGAAAATCAGAAGTTGGAGAGCTAGAGGTACGTAATATAGGTGGAGTTGA
GTTTTATTATTCTGTACGGGAATCCGTAGGTGTAAATAATCTCAATGAAAAGGTATGTGAAATACTACAGGATGCTGTAG
ATAATTTTCCTCTACATAAGAGAATGAAGTGGGGGTGCGGTATAGGGGAATGGGTGCGACCTGTTCTAAACGTCGCGTGC
GTGCTTGGTGGGGAAGTAGTCCCTGTAACGGTAGCTGGAAGTAAAGCTAATAATATAACTTACGGGAATATTAGGTTTGC
AAAGACTCCTCATACTATAAATGGAGTGGATAGCTATTTATCATTCTTGTCTGAGAACATGGTCATGTTGGACCATGAGG
AAAGGAGAAGAAGTATATTGAGGCAATTGGATGAGCAAATTGCTGGTACCCTTCTTAAGTATGATGTTCACGATAAAATA
ATAGAGGAGCTCAATGGAATGCTTGAGTATCCTAAGGTACTACTAGGAAGTGTAGATGAAAAATTCATGTCACTACCTAG
AGAGGTGGTAGCATGTGTACTAACTAAACACCAACGATGCTTGGCTATTTATGATAGTAGTGGAAGAATAGTCAAATTCG
CTTCTGTAGCTAGTGTTATCAATGAAAGGGTAATAAAAATTCATGAAATGGTATTGAGAGCAAGGCTTTCAGATGCCATG
TTTTTAATAGAAAAGGATAAGCAGTATGGTTTGGATAGGTATCGGGAGCAGCTTGCGAATATAGTATTTAAGAGAGATTT
GGGCTCGGTACTTGATAAAGTTAACAGAATATCTGCTCTTGCAAAATACGCTTCAGTATGGGTACCTAGGGCGCATATGT
TGGAAGTTGAGAGAGCATCTTTGCTTTCTAAGGCTGATCTTGCTTCGCTGATGGTGAAGGAGTTCCCTGAATTACAGGGA
AAAATGGGGAGGTATTACGCGCAACATGCTGGTGAAGAAAGTGCAGTGTGTGATGCGTTGGAAGAGCACTATTTACCTAG
TAGTCAACAGGATAGATGTCCTTCTTCTCCGGTAGGAATTGCTTTATCAATTGCGGATAAGGTAGACACTTTAGTGGGGT
TAATGGCTACAGAGAGAATAAGTGGTTCTCGTGATCCTTTTGCACTACGTAGAACTGCAATAGCTTTGCTGAGGGTAATA
AAGGAAAACTGTATATCGATACCGTTAGATCTGATGGTATCGAAGTCTGTATCTTTTTATCTTTGCGATGCGAAGAAGAA
TAAGGATTTAAAGTCAGTATTCAGAAGTGTTGATGTTGATTGTATAACTTCTTTAATATTAGATTTCTGTTATGAAAGAT
TGAAGATAATTCTCCGGGAAGAAGGGTTGGATAGAGATATCGTAAATATCGTTGTGGGAGAGTTTCATGATATATTATTG
GTAAAGAAAAAAGCAGTTATATTGAGTGAATATCTGAAAACAGAGGATGGTAAAAACATACTGGCCGCTTATAGGAGAAT
AAGAAACGTACTATTGAAGGGTAGTAGTGTTCCTGTAAAGGAAAAGAGGATATGGTATAAGTGGTGTAGTGAGAAGCTTT
GTATTGAGAGTTGTGAAATAGAGTTATATAGAAAGGCGAAACACTGCGATGGGGTAATTAGCTCGGTACTTAAGGCTCAT
AGTTTTAAGGAAGCCATGGATTGTCTTGCTAACATGAGTAGTGTGGTAAACGACTTTTTAGATAAGGTTAGAGTTCACGA
TGATGGAAGTATTGAGACGCGTAAGAATAGAGTAAATTTGAGTTTATGGGTCGTAAGTTTGTATAATTCTGTTATAGATT
TTAGTAAAATACCTAAGGAATCTTAA

Upstream 100 bases:

>100_bases
GTTATTGGCGTCAATGAACGGGCCTCTTACATACTCAGAGTTAGGAAAATGACTAATATGTGCTGCCAATCTTATGTAAA
GCTATACAAAGTAGCGTGAT

Downstream 100 bases:

>100_bases
ATTGTGATATTGAATATCGCAACTTTGATGTCTACATATAGACTGGCTTCTTTTTTATATGGTGTGAAGAAAATAAGATG
GGAAGTAATGACTATTACGA

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MSLDLVFEILCEQMPSKMMDIALGYLKNTICSGFSKFAIESRNSRTLVSANRICFIAEGLEQGNSTENKEIKGPRVSSSQ
ESIAGFLRKVGKSEVGELEVRNIGGVEFYYSVRESVGVNNLNEKVCEILQDAVDNFPLHKRMKWGCGIGEWVRPVLNVAC
VLGGEVVPVTVAGSKANNITYGNIRFAKTPHTINGVDSYLSFLSENMVMLDHEERRRSILRQLDEQIAGTLLKYDVHDKI
IEELNGMLEYPKVLLGSVDEKFMSLPREVVACVLTKHQRCLAIYDSSGRIVKFASVASVINERVIKIHEMVLRARLSDAM
FLIEKDKQYGLDRYREQLANIVFKRDLGSVLDKVNRISALAKYASVWVPRAHMLEVERASLLSKADLASLMVKEFPELQG
KMGRYYAQHAGEESAVCDALEEHYLPSSQQDRCPSSPVGIALSIADKVDTLVGLMATERISGSRDPFALRRTAIALLRVI
KENCISIPLDLMVSKSVSFYLCDAKKNKDLKSVFRSVDVDCITSLILDFCYERLKIILREEGLDRDIVNIVVGEFHDILL
VKKKAVILSEYLKTEDGKNILAAYRRIRNVLLKGSSVPVKEKRIWYKWCSEKLCIESCEIELYRKAKHCDGVISSVLKAH
SFKEAMDCLANMSSVVNDFLDKVRVHDDGSIETRKNRVNLSLWVVSLYNSVIDFSKIPKES

Sequences:

>Translated_701_residues
MSLDLVFEILCEQMPSKMMDIALGYLKNTICSGFSKFAIESRNSRTLVSANRICFIAEGLEQGNSTENKEIKGPRVSSSQ
ESIAGFLRKVGKSEVGELEVRNIGGVEFYYSVRESVGVNNLNEKVCEILQDAVDNFPLHKRMKWGCGIGEWVRPVLNVAC
VLGGEVVPVTVAGSKANNITYGNIRFAKTPHTINGVDSYLSFLSENMVMLDHEERRRSILRQLDEQIAGTLLKYDVHDKI
IEELNGMLEYPKVLLGSVDEKFMSLPREVVACVLTKHQRCLAIYDSSGRIVKFASVASVINERVIKIHEMVLRARLSDAM
FLIEKDKQYGLDRYREQLANIVFKRDLGSVLDKVNRISALAKYASVWVPRAHMLEVERASLLSKADLASLMVKEFPELQG
KMGRYYAQHAGEESAVCDALEEHYLPSSQQDRCPSSPVGIALSIADKVDTLVGLMATERISGSRDPFALRRTAIALLRVI
KENCISIPLDLMVSKSVSFYLCDAKKNKDLKSVFRSVDVDCITSLILDFCYERLKIILREEGLDRDIVNIVVGEFHDILL
VKKKAVILSEYLKTEDGKNILAAYRRIRNVLLKGSSVPVKEKRIWYKWCSEKLCIESCEIELYRKAKHCDGVISSVLKAH
SFKEAMDCLANMSSVVNDFLDKVRVHDDGSIETRKNRVNLSLWVVSLYNSVIDFSKIPKES
>Mature_700_residues
SLDLVFEILCEQMPSKMMDIALGYLKNTICSGFSKFAIESRNSRTLVSANRICFIAEGLEQGNSTENKEIKGPRVSSSQE
SIAGFLRKVGKSEVGELEVRNIGGVEFYYSVRESVGVNNLNEKVCEILQDAVDNFPLHKRMKWGCGIGEWVRPVLNVACV
LGGEVVPVTVAGSKANNITYGNIRFAKTPHTINGVDSYLSFLSENMVMLDHEERRRSILRQLDEQIAGTLLKYDVHDKII
EELNGMLEYPKVLLGSVDEKFMSLPREVVACVLTKHQRCLAIYDSSGRIVKFASVASVINERVIKIHEMVLRARLSDAMF
LIEKDKQYGLDRYREQLANIVFKRDLGSVLDKVNRISALAKYASVWVPRAHMLEVERASLLSKADLASLMVKEFPELQGK
MGRYYAQHAGEESAVCDALEEHYLPSSQQDRCPSSPVGIALSIADKVDTLVGLMATERISGSRDPFALRRTAIALLRVIK
ENCISIPLDLMVSKSVSFYLCDAKKNKDLKSVFRSVDVDCITSLILDFCYERLKIILREEGLDRDIVNIVVGEFHDILLV
KKKAVILSEYLKTEDGKNILAAYRRIRNVLLKGSSVPVKEKRIWYKWCSEKLCIESCEIELYRKAKHCDGVISSVLKAHS
FKEAMDCLANMSSVVNDFLDKVRVHDDGSIETRKNRVNLSLWVVSLYNSVIDFSKIPKES

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=716, Percent_Identity=27.2346368715084, Blast_Score=240, Evalue=2e-64,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78957; Mature: 78825

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDLVFEILCEQMPSKMMDIALGYLKNTICSGFSKFAIESRNSRTLVSANRICFIAEGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEECCCEEEEECHH
EQGNSTENKEIKGPRVSSSQESIAGFLRKVGKSEVGELEVRNIGGVEFYYSVRESVGVNN
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCC
LNEKVCEILQDAVDNFPLHKRMKWGCGIGEWVRPVLNVACVLGGEVVPVTVAGSKANNIT
HHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEE
YGNIRFAKTPHTINGVDSYLSFLSENMVMLDHEERRRSILRQLDEQIAGTLLKYDVHDKI
ECCEEEECCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEELNGMLEYPKVLLGSVDEKFMSLPREVVACVLTKHQRCLAIYDSSGRIVKFASVASVI
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHH
NERVIKIHEMVLRARLSDAMFLIEKDKQYGLDRYREQLANIVFKRDLGSVLDKVNRISAL
HHHHHHHHHHHHHHHHHHHHEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKYASVWVPRAHMLEVERASLLSKADLASLMVKEFPELQGKMGRYYAQHAGEESAVCDAL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EEHYLPSSQQDRCPSSPVGIALSIADKVDTLVGLMATERISGSRDPFALRRTAIALLRVI
HHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KENCISIPLDLMVSKSVSFYLCDAKKNKDLKSVFRSVDVDCITSLILDFCYERLKIILRE
HHHHHCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EGLDRDIVNIVVGEFHDILLVKKKAVILSEYLKTEDGKNILAAYRRIRNVLLKGSSVPVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCH
EKRIWYKWCSEKLCIESCEIELYRKAKHCDGVISSVLKAHSFKEAMDCLANMSSVVNDFL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKVRVHDDGSIETRKNRVNLSLWVVSLYNSVIDFSKIPKES
HHHEECCCCCCEECCCEEEHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLDLVFEILCEQMPSKMMDIALGYLKNTICSGFSKFAIESRNSRTLVSANRICFIAEGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEECCCEEEEECHH
EQGNSTENKEIKGPRVSSSQESIAGFLRKVGKSEVGELEVRNIGGVEFYYSVRESVGVNN
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCC
LNEKVCEILQDAVDNFPLHKRMKWGCGIGEWVRPVLNVACVLGGEVVPVTVAGSKANNIT
HHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEE
YGNIRFAKTPHTINGVDSYLSFLSENMVMLDHEERRRSILRQLDEQIAGTLLKYDVHDKI
ECCEEEECCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEELNGMLEYPKVLLGSVDEKFMSLPREVVACVLTKHQRCLAIYDSSGRIVKFASVASVI
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHH
NERVIKIHEMVLRARLSDAMFLIEKDKQYGLDRYREQLANIVFKRDLGSVLDKVNRISAL
HHHHHHHHHHHHHHHHHHHHEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKYASVWVPRAHMLEVERASLLSKADLASLMVKEFPELQGKMGRYYAQHAGEESAVCDAL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EEHYLPSSQQDRCPSSPVGIALSIADKVDTLVGLMATERISGSRDPFALRRTAIALLRVI
HHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KENCISIPLDLMVSKSVSFYLCDAKKNKDLKSVFRSVDVDCITSLILDFCYERLKIILRE
HHHHHCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EGLDRDIVNIVVGEFHDILLVKKKAVILSEYLKTEDGKNILAAYRRIRNVLLKGSSVPVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCH
EKRIWYKWCSEKLCIESCEIELYRKAKHCDGVISSVLKAHSFKEAMDCLANMSSVVNDFL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKVRVHDDGSIETRKNRVNLSLWVVSLYNSVIDFSKIPKES
HHHEECCCCCCEECCCEEEHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA