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The map label for this gene is thiD [H]

Identifier: 88606698

GI number: 88606698

Start: 1051289

End: 1052086

Strand: Reverse

Name: thiD [H]

Synonym: APH_0993

Alternate gene names: 88606698

Gene position: 1052086-1051289 (Counterclockwise)

Preceding gene: 88607408

Following gene: 88607551

Centisome position: 71.51

GC content: 45.11

Gene sequence:

>798_bases
TTGTATAAAGGTAATGTGCTTAGCATAGCTGGGTCTGATTCCGGTGGAGGAGCTGGTATTCAAGCTGACATAAAAACCAT
TACGATGTTGGGGTGCTACGCAGCTACATGCATTACCGCTATAACGGCGCAGAATACTCAAGAAGTTAGGGGAGTGTGGG
AGCTAGCTCCTGAAGTAGTAAAGGGGCAATTGGAAGCGGTACTGGATGACCTCAAGGTAGATGCTATAAAAATAGGTATG
CTGCCGGTTGGTTTGATAGGGGTGGTTGCTGAAAGTCTACCCTGCGAGATACCTGTGATACTGGATCCTGTGATGGTATC
GACGTCAGGTTTTGAATTAACAGACCCTATGGGATATACTGAAAGTATAAGACCGCTTGCACAGAATGCTGCTTTGGTTA
CACCCAATATTAAGGAGACCGAAGCGCTCTCAGGCATGAAAAATATCCGCACGCGGGAGGAAATGGTTACTGCAGGTAAC
AAGATAATTCAATTTCTAGGCATAAAGGCGGTTCTGGTTAAGGGTTGTATCTGGGAAGAGGAAGAGTTTGTAGAAAGCGT
ATTGATATACGATGGCAAGACTGTTTTCTTTAGAAATGAAAGAGCTCCTGGCGAGCTCCATGGCACAGGATGCACTTTAA
CATCAGCCATAGCGTGTTTCATAGCACGAGGGCTTTCACTGGAGGAGAGTACAAGGCTTGCATTTGACTATGTCTCGTGT
ACCATAAGAAACGCACCCATAGTAGGGCAGGGTTTAAGGCCTCTTTTTCACAACTATTTTATAGTAGGCGATTGTTAA

Upstream 100 bases:

>100_bases
AAGTTGAGTGATATTGTGCGTCTCATAGACATTGAATTGACAAGCTCGGCAGGCGGAGTTACAGTCCCGTTTGTGCGTTC
GATAATACGTAGAGTAGAGC

Downstream 100 bases:

>100_bases
AGTTTGAATCCTTGTTATAGGCTTGGTTCCCTTGCTGGCCAGGATAGGTGTTTAATAACACACGTCATGTTGTTACCTGT
ACGCCCATCTATAACACAGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MYKGNVLSIAGSDSGGGAGIQADIKTITMLGCYAATCITAITAQNTQEVRGVWELAPEVVKGQLEAVLDDLKVDAIKIGM
LPVGLIGVVAESLPCEIPVILDPVMVSTSGFELTDPMGYTESIRPLAQNAALVTPNIKETEALSGMKNIRTREEMVTAGN
KIIQFLGIKAVLVKGCIWEEEEFVESVLIYDGKTVFFRNERAPGELHGTGCTLTSAIACFIARGLSLEESTRLAFDYVSC
TIRNAPIVGQGLRPLFHNYFIVGDC

Sequences:

>Translated_265_residues
MYKGNVLSIAGSDSGGGAGIQADIKTITMLGCYAATCITAITAQNTQEVRGVWELAPEVVKGQLEAVLDDLKVDAIKIGM
LPVGLIGVVAESLPCEIPVILDPVMVSTSGFELTDPMGYTESIRPLAQNAALVTPNIKETEALSGMKNIRTREEMVTAGN
KIIQFLGIKAVLVKGCIWEEEEFVESVLIYDGKTVFFRNERAPGELHGTGCTLTSAIACFIARGLSLEESTRLAFDYVSC
TIRNAPIVGQGLRPLFHNYFIVGDC
>Mature_265_residues
MYKGNVLSIAGSDSGGGAGIQADIKTITMLGCYAATCITAITAQNTQEVRGVWELAPEVVKGQLEAVLDDLKVDAIKIGM
LPVGLIGVVAESLPCEIPVILDPVMVSTSGFELTDPMGYTESIRPLAQNAALVTPNIKETEALSGMKNIRTREEMVTAGN
KIIQFLGIKAVLVKGCIWEEEEFVESVLIYDGKTVFFRNERAPGELHGTGCTLTSAIACFIARGLSLEESTRLAFDYVSC
TIRNAPIVGQGLRPLFHNYFIVGDC

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=264, Percent_Identity=35.9848484848485, Blast_Score=155, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325378, Length=249, Percent_Identity=35.3413654618474, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=277, Percent_Identity=37.5451263537906, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324517, Length=277, Percent_Identity=36.8231046931408, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28421; Mature: 28421

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKGNVLSIAGSDSGGGAGIQADIKTITMLGCYAATCITAITAQNTQEVRGVWELAPEVV
CCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KGQLEAVLDDLKVDAIKIGMLPVGLIGVVAESLPCEIPVILDPVMVSTSGFELTDPMGYT
HHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHCCCCCCEEECCEEECCCCCEECCCCCCH
ESIRPLAQNAALVTPNIKETEALSGMKNIRTREEMVTAGNKIIQFLGIKAVLVKGCIWEE
HHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EEFVESVLIYDGKTVFFRNERAPGELHGTGCTLTSAIACFIARGLSLEESTRLAFDYVSC
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHEEEEEEE
TIRNAPIVGQGLRPLFHNYFIVGDC
EEECCCCCCCCCHHHHHCEEEEECC
>Mature Secondary Structure
MYKGNVLSIAGSDSGGGAGIQADIKTITMLGCYAATCITAITAQNTQEVRGVWELAPEVV
CCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KGQLEAVLDDLKVDAIKIGMLPVGLIGVVAESLPCEIPVILDPVMVSTSGFELTDPMGYT
HHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHCCCCCCEEECCEEECCCCCEECCCCCCH
ESIRPLAQNAALVTPNIKETEALSGMKNIRTREEMVTAGNKIIQFLGIKAVLVKGCIWEE
HHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EEFVESVLIYDGKTVFFRNERAPGELHGTGCTLTSAIACFIARGLSLEESTRLAFDYVSC
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHEEEEEEE
TIRNAPIVGQGLRPLFHNYFIVGDC
EEECCCCCCCCCHHHHHCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]