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The map label for this gene is cnrH [H]

Identifier: 87200940

GI number: 87200940

Start: 3143341

End: 3143904

Strand: Direct

Name: cnrH [H]

Synonym: Saro_2928

Alternate gene names: 87200940

Gene position: 3143341-3143904 (Clockwise)

Preceding gene: 87200939

Following gene: 87200941

Centisome position: 88.26

GC content: 66.13

Gene sequence:

>564_bases
ATGAACGACACCGGGACCGACGTTGGCGATCGCGAACTGGTCTCGCGCGCGCTCTCAGGACAGCAGAGCGCGTACACGCA
CCTGATGGACCGCCATCGCACCGTCGTCTTCCGCGTGGTGCGCCATCACACCGGCGACGAGAGCGAGGCGCTCGACCTCA
CGCAGGAGGCGTTCATCTCGGCGTTCGCTTCGCTGAACAGGTTCGACCCCGGCCGCCCCTTCCGCACCTGGCTGCTGCGG
ATCGCCATCAACAAGTGCCGCGATTGGGCGCGCCGCCGCGCGGTGCGGCGCCTGTTCCGCATGGCGCGCCCGCTGGAAGA
GGCGTTCGACATCGCCGACCAGGGGCAGGACCCTGAATCCGAAGCGATTTCGCGGCGCGAGCTGGCCCGGATATCCGGCG
CGATCGCTTCGCTGCCGGCCAATCTCAAGGACGCGCTGATCCTTTGCCGGATCGAAGGGCTGAGCATGGCCGAAGCCGCG
CAGGTCCTGGGCCTTTCGGAAAAGGCCGTGGAAACGCGCATTTACCGGGCAAGGCAGAAATTGTCGGAAATTCTTGAGGG
ATAG

Upstream 100 bases:

>100_bases
CGTGTTCGCCATGAGATCGGTACTAAACCCTTCTCAACAGGCGATTTTTGACCAGGAAATCAGCCGCGCGCTGACTGCGC
CGAACGACACCTAAACGTAC

Downstream 100 bases:

>100_bases
TTCCCGCGCGTGCGTATTCGCAATCGATGGACAAGAACCTCCTGCTCGACCGCCGCCGCCTGCTTGTCGGCTCCGGCGCC
CTGGCCCTCGCGCCGCTGTT

Product: FecI-like sigma-24 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNDTGTDVGDRELVSRALSGQQSAYTHLMDRHRTVVFRVVRHHTGDESEALDLTQEAFISAFASLNRFDPGRPFRTWLLR
IAINKCRDWARRRAVRRLFRMARPLEEAFDIADQGQDPESEAISRRELARISGAIASLPANLKDALILCRIEGLSMAEAA
QVLGLSEKAVETRIYRARQKLSEILEG

Sequences:

>Translated_187_residues
MNDTGTDVGDRELVSRALSGQQSAYTHLMDRHRTVVFRVVRHHTGDESEALDLTQEAFISAFASLNRFDPGRPFRTWLLR
IAINKCRDWARRRAVRRLFRMARPLEEAFDIADQGQDPESEAISRRELARISGAIASLPANLKDALILCRIEGLSMAEAA
QVLGLSEKAVETRIYRARQKLSEILEG
>Mature_187_residues
MNDTGTDVGDRELVSRALSGQQSAYTHLMDRHRTVVFRVVRHHTGDESEALDLTQEAFISAFASLNRFDPGRPFRTWLLR
IAINKCRDWARRRAVRRLFRMARPLEEAFDIADQGQDPESEAISRRELARISGAIASLPANLKDALILCRIEGLSMAEAA
QVLGLSEKAVETRIYRARQKLSEILEG

Specific function: CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in a RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigm

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=176, Percent_Identity=30.1136363636364, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21147; Mature: 21147

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTGTDVGDRELVSRALSGQQSAYTHLMDRHRTVVFRVVRHHTGDESEALDLTQEAFIS
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFASLNRFDPGRPFRTWLLRIAINKCRDWARRRAVRRLFRMARPLEEAFDIADQGQDPES
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EAISRRELARISGAIASLPANLKDALILCRIEGLSMAEAAQVLGLSEKAVETRIYRARQK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
LSEILEG
HHHHHCC
>Mature Secondary Structure
MNDTGTDVGDRELVSRALSGQQSAYTHLMDRHRTVVFRVVRHHTGDESEALDLTQEAFIS
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFASLNRFDPGRPFRTWLLRIAINKCRDWARRRAVRRLFRMARPLEEAFDIADQGQDPES
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EAISRRELARISGAIASLPANLKDALILCRIEGLSMAEAAQVLGLSEKAVETRIYRARQK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
LSEILEG
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380802; 8052622; 10671463; 10671464 [H]