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The map label for this gene is cysS

Identifier: 87200394

GI number: 87200394

Start: 2533879

End: 2535360

Strand: Reverse

Name: cysS

Synonym: Saro_2381

Alternate gene names: 87200394

Gene position: 2535360-2533879 (Counterclockwise)

Preceding gene: 87200395

Following gene: 87200393

Centisome position: 71.19

GC content: 65.05

Gene sequence:

>1482_bases
ATGTCCGTCCACGCTCCCGCGCCGTTCGCGCCTTCGGCACCGCTCCGGCTGTTCAATAGCCTCACCCGCCAGCTGGAAGC
GTTCCAGCCGGTCCACGCGGGCGAGGCGCGCGTCTATTCGTGCGGGCCGACCGTGTACAACTACCCGCACATCGGCAACA
TGCGGGCCTATGTCTTCGCCGATCTGCTCGGGCGTACGCTGTCGTTCAAGGGGCTGAAGCTTACTCACGTCATCAACATC
ACCGACGTCGGCCACCTGACCGACGATGCCGACGAGGGCGAGGACAAGATGGAGAAGATGGCGCGGGCCGAGGCGCGCTC
CATCTGGGACATCGCGAAGCACTATACCCAGGCCTATTGGGAGGACGTGAAGGCGCTCAACATCCGCCAGCCCGCGCGCT
GGACGATTGCCACCGAATACGTGCCCCAGATGATTGCCTTTGCCGAGCGGATCGCGGCGAAGCATTGCTACGAACTCGAC
AGCGGGCTCTATTTCGATGTCTCTACGGTCGCCGATTACGGCCGTCTGGCCCGCGCCCAGACCGAAGAGGGCGAGGGGCG
CATCGAGGCGGTCGAAGGCAAGCGCAACGGCGCCGACTTCGCCATCTGGCGCAAGACGCCGCCGGGCGAGAAACGCCAGA
TGGAATGGGATTCGCCATGGGGCAGGGGCGCCCCGGGCTGGCATCTCGAATGCTCGGTCATGTCGGGCGAAGTCCTCGGA
TTTCCGTTCGACATCCACACCGGCGGCATCGACCACCGCGAGATCCACCACCCGAACGAGATCGCGCAGAACCAGGCGTT
CTGCTGCACCAATGGCTTGGACGTGCCGGCCAATTCGGGCGCGCGCATCTGGATGCACAACAACTTCCTGGTCGAGCGCT
CGGGCAAGATGAGCAAGTCGAGCGGCGAGTTCCTGCGCCTGCAGCTGCTGATCGACAAGGGTTATCACCCTCTGGCCTAC
CGCCTCATGTGCCTCCAGGCCCACTATCGCAGCGAACTCGAGTTCTCGTGGGAGGGCCTTGGCGCCGCACTGACGCGGCT
CAAGCGGATGCTGATCGCGGTGGGCCAGCTCAAGGAGCGTGCGCCGGAAGTCGCAGCGCAGCCGGGGCCGCGCTTCACGC
CGTTCCTCGAAAGGTTCGACGCGGCTATGTCGGACGACCTCAATACCGCCGTCGCACTGACCGTTTTCGAGGAAGTGCTC
GGGCTGAAGAAGGTCGATCCGGCGGACAAGCTTGCCGCTGTCGCCGCGATGGACGCCGTCCTCGGGCTCGGGCTGCTTGA
ACTCGACCGCAGGGCGCTGCGCATCCGGCCCAAGGGCGTCGAGATCGACGAGGCTGGGATCGAAGCCCTGCTAGATGCTC
GCAAGGCGGCCCGTGCCGAAAAGGACTTTGCCCGCTCTGACGCACTTCGCGACCAGATTGCTGCCAAGGGCGTGGAAGTG
ATGGACGGCGATCCGCTCGGCTGGGAGTGGAAACTCGCGTAA

Upstream 100 bases:

>100_bases
TCGACGAGCCGGTGACAGGCACCGGAAACGACGACCTGCTGGGCGATGAAGAAGGTGAGTGACTTGACCTGATGCTCGGG
AGCGGGGCAAGGGCAGGGCC

Downstream 100 bases:

>100_bases
CCAGTTTCATTTCAGGACCGGCGCGCTAATCTCTCCGCCGACACAACCGGAGAGTGCCGCAAAGTGACAGTTCTTGTGAT
GAATGCCGTCGCGGCGCCGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSVHAPAPFAPSAPLRLFNSLTRQLEAFQPVHAGEARVYSCGPTVYNYPHIGNMRAYVFADLLGRTLSFKGLKLTHVINI
TDVGHLTDDADEGEDKMEKMARAEARSIWDIAKHYTQAYWEDVKALNIRQPARWTIATEYVPQMIAFAERIAAKHCYELD
SGLYFDVSTVADYGRLARAQTEEGEGRIEAVEGKRNGADFAIWRKTPPGEKRQMEWDSPWGRGAPGWHLECSVMSGEVLG
FPFDIHTGGIDHREIHHPNEIAQNQAFCCTNGLDVPANSGARIWMHNNFLVERSGKMSKSSGEFLRLQLLIDKGYHPLAY
RLMCLQAHYRSELEFSWEGLGAALTRLKRMLIAVGQLKERAPEVAAQPGPRFTPFLERFDAAMSDDLNTAVALTVFEEVL
GLKKVDPADKLAAVAAMDAVLGLGLLELDRRALRIRPKGVEIDEAGIEALLDARKAARAEKDFARSDALRDQIAAKGVEV
MDGDPLGWEWKLA

Sequences:

>Translated_493_residues
MSVHAPAPFAPSAPLRLFNSLTRQLEAFQPVHAGEARVYSCGPTVYNYPHIGNMRAYVFADLLGRTLSFKGLKLTHVINI
TDVGHLTDDADEGEDKMEKMARAEARSIWDIAKHYTQAYWEDVKALNIRQPARWTIATEYVPQMIAFAERIAAKHCYELD
SGLYFDVSTVADYGRLARAQTEEGEGRIEAVEGKRNGADFAIWRKTPPGEKRQMEWDSPWGRGAPGWHLECSVMSGEVLG
FPFDIHTGGIDHREIHHPNEIAQNQAFCCTNGLDVPANSGARIWMHNNFLVERSGKMSKSSGEFLRLQLLIDKGYHPLAY
RLMCLQAHYRSELEFSWEGLGAALTRLKRMLIAVGQLKERAPEVAAQPGPRFTPFLERFDAAMSDDLNTAVALTVFEEVL
GLKKVDPADKLAAVAAMDAVLGLGLLELDRRALRIRPKGVEIDEAGIEALLDARKAARAEKDFARSDALRDQIAAKGVEV
MDGDPLGWEWKLA
>Mature_492_residues
SVHAPAPFAPSAPLRLFNSLTRQLEAFQPVHAGEARVYSCGPTVYNYPHIGNMRAYVFADLLGRTLSFKGLKLTHVINIT
DVGHLTDDADEGEDKMEKMARAEARSIWDIAKHYTQAYWEDVKALNIRQPARWTIATEYVPQMIAFAERIAAKHCYELDS
GLYFDVSTVADYGRLARAQTEEGEGRIEAVEGKRNGADFAIWRKTPPGEKRQMEWDSPWGRGAPGWHLECSVMSGEVLGF
PFDIHTGGIDHREIHHPNEIAQNQAFCCTNGLDVPANSGARIWMHNNFLVERSGKMSKSSGEFLRLQLLIDKGYHPLAYR
LMCLQAHYRSELEFSWEGLGAALTRLKRMLIAVGQLKERAPEVAAQPGPRFTPFLERFDAAMSDDLNTAVALTVFEEVLG
LKKVDPADKLAAVAAMDAVLGLGLLELDRRALRIRPKGVEIDEAGIEALLDARKAARAEKDFARSDALRDQIAAKGVEVM
DGDPLGWEWKLA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=337, Percent_Identity=34.7181008902077, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI10835051, Length=247, Percent_Identity=33.1983805668016, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI303304987, Length=247, Percent_Identity=33.1983805668016, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI62240992, Length=247, Percent_Identity=33.1983805668016, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI21269877, Length=247, Percent_Identity=33.1983805668016, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1786737, Length=487, Percent_Identity=37.5770020533881, Blast_Score=248, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17509795, Length=257, Percent_Identity=31.5175097276265, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17509797, Length=257, Percent_Identity=31.5175097276265, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324082, Length=316, Percent_Identity=30.6962025316456, Blast_Score=140, Evalue=7e-34,
Organism=Drosophila melanogaster, GI19922218, Length=503, Percent_Identity=29.4234592445328, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI19922364, Length=323, Percent_Identity=30.3405572755418, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_NOVAD (Q2G5Q6)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497651.1
- ProteinModelPortal:   Q2G5Q6
- SMR:   Q2G5Q6
- STRING:   Q2G5Q6
- GeneID:   3915726
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2381
- NMPDR:   fig|48935.1.peg.2439
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   EKRQMEW
- ProtClustDB:   CLSK835090
- BioCyc:   NARO279238:SARO_2381-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54955; Mature: 54824

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHAPAPFAPSAPLRLFNSLTRQLEAFQPVHAGEARVYSCGPTVYNYPHIGNMRAYVFA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
DLLGRTLSFKGLKLTHVINITDVGHLTDDADEGEDKMEKMARAEARSIWDIAKHYTQAYW
HHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDVKALNIRQPARWTIATEYVPQMIAFAERIAAKHCYELDSGLYFDVSTVADYGRLARAQ
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCC
TEEGEGRIEAVEGKRNGADFAIWRKTPPGEKRQMEWDSPWGRGAPGWHLECSVMSGEVLG
CCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEE
FPFDIHTGGIDHREIHHPNEIAQNQAFCCTNGLDVPANSGARIWMHNNFLVERSGKMSKS
CEEEECCCCCCCHHCCCCHHHHCCCCEEECCCCCCCCCCCCEEEEECCEEEECCCCCCCC
SGEFLRLQLLIDKGYHPLAYRLMCLQAHYRSELEFSWEGLGAALTRLKRMLIAVGQLKER
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
APEVAAQPGPRFTPFLERFDAAMSDDLNTAVALTVFEEVLGLKKVDPADKLAAVAAMDAV
CCHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGLGLLELDRRALRIRPKGVEIDEAGIEALLDARKAARAEKDFARSDALRDQIAAKGVEV
HHCHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
MDGDPLGWEWKLA
ECCCCCCEEEEEC
>Mature Secondary Structure 
SVHAPAPFAPSAPLRLFNSLTRQLEAFQPVHAGEARVYSCGPTVYNYPHIGNMRAYVFA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
DLLGRTLSFKGLKLTHVINITDVGHLTDDADEGEDKMEKMARAEARSIWDIAKHYTQAYW
HHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDVKALNIRQPARWTIATEYVPQMIAFAERIAAKHCYELDSGLYFDVSTVADYGRLARAQ
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCC
TEEGEGRIEAVEGKRNGADFAIWRKTPPGEKRQMEWDSPWGRGAPGWHLECSVMSGEVLG
CCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEE
FPFDIHTGGIDHREIHHPNEIAQNQAFCCTNGLDVPANSGARIWMHNNFLVERSGKMSKS
CEEEECCCCCCCHHCCCCHHHHCCCCEEECCCCCCCCCCCCEEEEECCEEEECCCCCCCC
SGEFLRLQLLIDKGYHPLAYRLMCLQAHYRSELEFSWEGLGAALTRLKRMLIAVGQLKER
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
APEVAAQPGPRFTPFLERFDAAMSDDLNTAVALTVFEEVLGLKKVDPADKLAAVAAMDAV
CCHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGLGLLELDRRALRIRPKGVEIDEAGIEALLDARKAARAEKDFARSDALRDQIAAKGVEV
HHCHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
MDGDPLGWEWKLA
ECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA