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The map label for this gene is gspE [C]

Identifier: 87199873

GI number: 87199873

Start: 1955826

End: 1957391

Strand: Reverse

Name: gspE [C]

Synonym: Saro_1856

Alternate gene names: 87199873

Gene position: 1957391-1955826 (Counterclockwise)

Preceding gene: 87199875

Following gene: 87199871

Centisome position: 54.96

GC content: 64.24

Gene sequence:

>1566_bases
ATGAGCGCTTTCGGGCGAAAGAACGGCATTGCAGGCAATTCCGGCGGAGGTCGCCCCTCGTTCGGCGTGGCCCGCCCGAT
GCGTGGAGGCGAAGCCGGGGCCACGGGCGCCGCGCCGTCGTCGGCTTCCACGCCGATGCCCGTCCCGATGCCCATGCCGA
TGGGAGGGGACCAGTTCCCGCCGATCCCGTCGATGGAGGACATGGACGCCCCGTCGAGCGCGGCTGCCCTGAAGGCCGAT
GCGCTGTCGCGCCTGGCGGACCGCGCCAACGCGGTTGCCGAGGGCAATTACCAGGCCGAAGGGTTCGAAGCCTCGGTCCA
CAAGATCAAGGAACAGGTGCTGCCGCGCCTGCTCGAGCGCGTCGATCCGGAAGCGGCCGCGACCCTGACCAAGGACGAGC
TGTCCGAGGAATTCCGGCCGATCATCATGGAAGTGCTGGCCGAGCTGAAGGTCACGCTGAACAGGCGCGAGCAGTTCGCG
CTGGAAAAGGTGCTGATCGACGAGCTGCTCGGCTTCGGTCCGCTGGAAGAGCTGCTCAACGACCCGGACGTTTCCGACAT
CATGGTCAACGGACCTGAGCAGACCTACATCGAAAAGAAGGGCAAGCTGCAACTTGCCCCGATCCGTTTTCGCGACGAAA
GCCACCTGTTCCAGATCGCCCAGCGCATCGTCAACCAGGTCGGCCGCCGCGTCGACCAGACCACGCCGCTGGCCGACGCC
CGCCTCAAGGACGGCAGCCGCGTCAACGTGATCGTGCCGCCGCTGTCGCTGCGCGGCACCGCGATCTCGATCCGCAAGTT
CTCCGAAAAGCCGATCACGATCGACATGCTGCGCGACTTCGGCTCGATGTCGGACAAGATGGCGACCTGCCTCAAGATCG
CCGGCGCAAGCCGCATGAACGTGGTCATCTCGGGCGGTACCGGTTCGGGCAAGACGACGATGCTCAACGCCCTGTCGAAG
ATGATCGACCCGGGCGAGCGCGTGTTGACCATCGAGGACGCGGCCGAACTCCGCTTGCAGCAGCCGCACTGGCTTCCGCT
GGAAACGCGTCCGCCGAACCTTGAAGGCCAGGGTGCGATCACCATCGGCGACCTTGTGAAGAACGCGCTGCGCATGCGTC
CCGACCGCATCATCCTGGGCGAAATCCGTGGCGCAGAATGCTTCGACCTTCTGGCGGCGATGAACACCGGCCACGACGGG
TCGATGTGCACGCTTCACGCCAACAGCCCGCGCGAATGCCTTGGCCGTATGGAAAACATGATCCTGATGGGCGATATCAA
GATCCCCAAGGAGGCCATCAGCCGCCAGATCGCGGAATCGGTCGACCTGATCGTTCAGGTCAAGCGCCTGCGCGATGGTT
CGCGTCGCACGACCAACATCACCGAAGTGATCGGGATGGAAGGCGACGTGATCGTGACGCAGGAACTGTTCAAGTTCGAA
TATCTGGACGAGACCGACGAAGGCAAGATCATCGGCGAGTTCCGGTCCTCCGGCCTGCGCCCATACACTCTTGAAAAGGC
GCGCCAGTTCGGGTTCGACCAGGCCTATCTCGAGGCCTGCCTCTAG

Upstream 100 bases:

>100_bases
ACCATGACCCGCGCATAGACCGCCATCTGCCGTCAACGGTTAGCCTTTCATAAAAGATTGGCGCGTAGAAAAATCGGCAG
ATGGACGGGAAGATTGCACG

Downstream 100 bases:

>100_bases
GCCGTAAACGGCCTAGGCCGCGAACCACTCCCGCGCCGCGAGGCCGAGGAGAATACAGGCGGCAAGGAAGGCGATCAGGT
AGATCACCGTCCACGGCATG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSAFGRKNGIAGNSGGGRPSFGVARPMRGGEAGATGAAPSSASTPMPVPMPMPMGGDQFPPIPSMEDMDAPSSAAALKAD
ALSRLADRANAVAEGNYQAEGFEASVHKIKEQVLPRLLERVDPEAAATLTKDELSEEFRPIIMEVLAELKVTLNRREQFA
LEKVLIDELLGFGPLEELLNDPDVSDIMVNGPEQTYIEKKGKLQLAPIRFRDESHLFQIAQRIVNQVGRRVDQTTPLADA
RLKDGSRVNVIVPPLSLRGTAISIRKFSEKPITIDMLRDFGSMSDKMATCLKIAGASRMNVVISGGTGSGKTTMLNALSK
MIDPGERVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDG
SMCTLHANSPRECLGRMENMILMGDIKIPKEAISRQIAESVDLIVQVKRLRDGSRRTTNITEVIGMEGDVIVTQELFKFE
YLDETDEGKIIGEFRSSGLRPYTLEKARQFGFDQAYLEACL

Sequences:

>Translated_521_residues
MSAFGRKNGIAGNSGGGRPSFGVARPMRGGEAGATGAAPSSASTPMPVPMPMPMGGDQFPPIPSMEDMDAPSSAAALKAD
ALSRLADRANAVAEGNYQAEGFEASVHKIKEQVLPRLLERVDPEAAATLTKDELSEEFRPIIMEVLAELKVTLNRREQFA
LEKVLIDELLGFGPLEELLNDPDVSDIMVNGPEQTYIEKKGKLQLAPIRFRDESHLFQIAQRIVNQVGRRVDQTTPLADA
RLKDGSRVNVIVPPLSLRGTAISIRKFSEKPITIDMLRDFGSMSDKMATCLKIAGASRMNVVISGGTGSGKTTMLNALSK
MIDPGERVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDG
SMCTLHANSPRECLGRMENMILMGDIKIPKEAISRQIAESVDLIVQVKRLRDGSRRTTNITEVIGMEGDVIVTQELFKFE
YLDETDEGKIIGEFRSSGLRPYTLEKARQFGFDQAYLEACL
>Mature_520_residues
SAFGRKNGIAGNSGGGRPSFGVARPMRGGEAGATGAAPSSASTPMPVPMPMPMGGDQFPPIPSMEDMDAPSSAAALKADA
LSRLADRANAVAEGNYQAEGFEASVHKIKEQVLPRLLERVDPEAAATLTKDELSEEFRPIIMEVLAELKVTLNRREQFAL
EKVLIDELLGFGPLEELLNDPDVSDIMVNGPEQTYIEKKGKLQLAPIRFRDESHLFQIAQRIVNQVGRRVDQTTPLADAR
LKDGSRVNVIVPPLSLRGTAISIRKFSEKPITIDMLRDFGSMSDKMATCLKIAGASRMNVVISGGTGSGKTTMLNALSKM
IDPGERVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGS
MCTLHANSPRECLGRMENMILMGDIKIPKEAISRQIAESVDLIVQVKRLRDGSRRTTNITEVIGMEGDVIVTQELFKFEY
LDETDEGKIIGEFRSSGLRPYTLEKARQFGFDQAYLEACL

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 56910; Mature: 56779

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFGRKNGIAGNSGGGRPSFGVARPMRGGEAGATGAAPSSASTPMPVPMPMPMGGDQFP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PIPSMEDMDAPSSAAALKADALSRLADRANAVAEGNYQAEGFEASVHKIKEQVLPRLLER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VDPEAAATLTKDELSEEFRPIIMEVLAELKVTLNRREQFALEKVLIDELLGFGPLEELLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
DPDVSDIMVNGPEQTYIEKKGKLQLAPIRFRDESHLFQIAQRIVNQVGRRVDQTTPLADA
CCCCCCEEECCCHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RLKDGSRVNVIVPPLSLRGTAISIRKFSEKPITIDMLRDFGSMSDKMATCLKIAGASRMN
HCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEHHHHHHHCCHHHHHHHHHHHCCCCEEE
VVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAI
EEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCE
TIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGSMCTLHANSPRECLGRMENM
EHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCE
ILMGDIKIPKEAISRQIAESVDLIVQVKRLRDGSRRTTNITEVIGMEGDVIVTQELFKFE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEHHHHHHHH
YLDETDEGKIIGEFRSSGLRPYTLEKARQFGFDQAYLEACL
HCCCCCCCCEEEHHHHCCCCCHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
SAFGRKNGIAGNSGGGRPSFGVARPMRGGEAGATGAAPSSASTPMPVPMPMPMGGDQFP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PIPSMEDMDAPSSAAALKADALSRLADRANAVAEGNYQAEGFEASVHKIKEQVLPRLLER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VDPEAAATLTKDELSEEFRPIIMEVLAELKVTLNRREQFALEKVLIDELLGFGPLEELLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
DPDVSDIMVNGPEQTYIEKKGKLQLAPIRFRDESHLFQIAQRIVNQVGRRVDQTTPLADA
CCCCCCEEECCCHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RLKDGSRVNVIVPPLSLRGTAISIRKFSEKPITIDMLRDFGSMSDKMATCLKIAGASRMN
HCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEHHHHHHHCCHHHHHHHHHHHCCCCEEE
VVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLPLETRPPNLEGQGAI
EEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCE
TIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGSMCTLHANSPRECLGRMENM
EHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCE
ILMGDIKIPKEAISRQIAESVDLIVQVKRLRDGSRRTTNITEVIGMEGDVIVTQELFKFE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEHHHHHHHH
YLDETDEGKIIGEFRSSGLRPYTLEKARQFGFDQAYLEACL
HCCCCCCCCEEEHHHHCCCCCHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]