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The map label for this gene is rbsR [H]

Identifier: 87199745

GI number: 87199745

Start: 1820365

End: 1821411

Strand: Reverse

Name: rbsR [H]

Synonym: Saro_1728

Alternate gene names: 87199745

Gene position: 1821411-1820365 (Counterclockwise)

Preceding gene: 87199750

Following gene: 87199738

Centisome position: 51.14

GC content: 64.28

Gene sequence:

>1047_bases
ATGGGTGAAATGAACATCGCAAGTACGAGCCGCGGCAACGCACACGCAACGATGGAGGACGTGGCAAAACTCGCTGGCGT
CTCGCTAAAGAGCGTGTCTCGCGTAATCAACGCCGAGCCGCACGTATCGGCAAAGCTGAGGGCAAAGGTCGAGGCGGCGA
TTGCCGAACTCAATTACGTTCCGGATACGGCAGCGCGCTCGCTTGCCGGATCGCGGGCTTTCATCGTCGGCCTGCTGTTC
GACAACCCCAGCCCGAACTACACGATGAACATCCAGAAGGGCGTGTACGAGACCTGCCGCGACCAGCAGCACCACCTGCG
CATCGACAACATCGATTCGACGGTTCCCGCCGAAAAGTTCGAGGCACAACTGGCGGCGATGGTGCGCAACAGCCGATGCG
ACGGGTTCGTGCTAACGCCTCCCCTTACCGACAACGTGGTGCTACTCGATTTCCTCGACCGTAGCGGCATCCGCTATGTA
CGCATTGCGCCGGACATTCAGCCCGACCGATCGCCCGGGGTCTGCATCGATGACGCGGCAGCAGCAGCCGCCGCCGCGCG
CCACCTGTGGGAACTGGGGCACAGGCGCTTTGCCGTGGTGCGCGGGCCCGCCAGCCACGGCGCGGCGGGACGGCGACGCC
AAGGCTTCATCGACGAGTTGCACAGGCTCGGCGCGGAGAACCCCATCATCGAGGCGGAAGGCAATTTCAGCTTCGAAAGC
GGCATCGCAGCGGGTGCGAAGGTTCTGGCGGCAACCCCCCGCCCGACCGCGATCTTTGCCGCGAACGACGATTCGGCCGC
AGGCGTCATGGTCGCCTGCTCGCAGGCCGGACTGAAAGTGCCGAACGACGTTTCAGTCTGCGGCTTCGACGATAGCTGGG
TGGCGAAGTCGGTCTGGCCCTATCTGACCACCGTCTACCAGCCCATCGAGGAGATGGGCCGGGCCGCCGCGGCGCTGTTG
CTACGCCGCGACGAGCCCGACAATGTCCTCCACGAACTGGATTTCAGTCTCGTCGTCAGGGCTTCGACGGCACCCCCGCC
CCAATAG

Upstream 100 bases:

>100_bases
CATGATATCGACACCGGCGCGTGAGATTGCGGAATGGACACGACAAGGCTGATGACGAAATCAATGGATCGGCTAAAAGG
CCGCCAGCACGAAGATCGGC

Downstream 100 bases:

>100_bases
CTTCAAGCGTCACCGGCCCGATCAGGCCCGAGGGCCGCAAAGGCGCGTCGGGGCCATAGGTCGGGAGCGCCGTCCAGGTG
ATCCGGGACGCATTGGGCTG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MGEMNIASTSRGNAHATMEDVAKLAGVSLKSVSRVINAEPHVSAKLRAKVEAAIAELNYVPDTAARSLAGSRAFIVGLLF
DNPSPNYTMNIQKGVYETCRDQQHHLRIDNIDSTVPAEKFEAQLAAMVRNSRCDGFVLTPPLTDNVVLLDFLDRSGIRYV
RIAPDIQPDRSPGVCIDDAAAAAAAARHLWELGHRRFAVVRGPASHGAAGRRRQGFIDELHRLGAENPIIEAEGNFSFES
GIAAGAKVLAATPRPTAIFAANDDSAAGVMVACSQAGLKVPNDVSVCGFDDSWVAKSVWPYLTTVYQPIEEMGRAAAALL
LRRDEPDNVLHELDFSLVVRASTAPPPQ

Sequences:

>Translated_348_residues
MGEMNIASTSRGNAHATMEDVAKLAGVSLKSVSRVINAEPHVSAKLRAKVEAAIAELNYVPDTAARSLAGSRAFIVGLLF
DNPSPNYTMNIQKGVYETCRDQQHHLRIDNIDSTVPAEKFEAQLAAMVRNSRCDGFVLTPPLTDNVVLLDFLDRSGIRYV
RIAPDIQPDRSPGVCIDDAAAAAAAARHLWELGHRRFAVVRGPASHGAAGRRRQGFIDELHRLGAENPIIEAEGNFSFES
GIAAGAKVLAATPRPTAIFAANDDSAAGVMVACSQAGLKVPNDVSVCGFDDSWVAKSVWPYLTTVYQPIEEMGRAAAALL
LRRDEPDNVLHELDFSLVVRASTAPPPQ
>Mature_347_residues
GEMNIASTSRGNAHATMEDVAKLAGVSLKSVSRVINAEPHVSAKLRAKVEAAIAELNYVPDTAARSLAGSRAFIVGLLFD
NPSPNYTMNIQKGVYETCRDQQHHLRIDNIDSTVPAEKFEAQLAAMVRNSRCDGFVLTPPLTDNVVLLDFLDRSGIRYVR
IAPDIQPDRSPGVCIDDAAAAAAAARHLWELGHRRFAVVRGPASHGAAGRRRQGFIDELHRLGAENPIIEAEGNFSFESG
IAAGAKVLAATPRPTAIFAANDDSAAGVMVACSQAGLKVPNDVSVCGFDDSWVAKSVWPYLTTVYQPIEEMGRAAAALLL
RRDEPDNVLHELDFSLVVRASTAPPPQ

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=315, Percent_Identity=33.6507936507937, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786540, Length=347, Percent_Identity=32.8530259365994, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=32.258064516129, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=29.4117647058824, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788474, Length=315, Percent_Identity=31.7460317460317, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=32.6666666666667, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789068, Length=297, Percent_Identity=27.9461279461279, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=26.9938650306748, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=25.3968253968254, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787580, Length=326, Percent_Identity=23.9263803680982, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790689, Length=326, Percent_Identity=25.1533742331288, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37373; Mature: 37241

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEMNIASTSRGNAHATMEDVAKLAGVSLKSVSRVINAEPHVSAKLRAKVEAAIAELNYV
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
PDTAARSLAGSRAFIVGLLFDNPSPNYTMNIQKGVYETCRDQQHHLRIDNIDSTVPAEKF
CCHHHHHHCCCCEEEEEEEECCCCCCEEEEEHHHHHHHHCCCHHCEEECCCCCCCCHHHH
EAQLAAMVRNSRCDGFVLTPPLTDNVVLLDFLDRSGIRYVRIAPDIQPDRSPGVCIDDAA
HHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCEECHHH
AAAAAARHLWELGHRRFAVVRGPASHGAAGRRRQGFIDELHRLGAENPIIEAEGNFSFES
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCHHHHHHHCCCCCCEEECCCCCCHHC
GIAAGAKVLAATPRPTAIFAANDDSAAGVMVACSQAGLKVPNDVSVCGFDDSWVAKSVWP
CHHCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHH
YLTTVYQPIEEMGRAAAALLLRRDEPDNVLHELDFSLVVRASTAPPPQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEECCCCCCC
>Mature Secondary Structure 
GEMNIASTSRGNAHATMEDVAKLAGVSLKSVSRVINAEPHVSAKLRAKVEAAIAELNYV
CCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
PDTAARSLAGSRAFIVGLLFDNPSPNYTMNIQKGVYETCRDQQHHLRIDNIDSTVPAEKF
CCHHHHHHCCCCEEEEEEEECCCCCCEEEEEHHHHHHHHCCCHHCEEECCCCCCCCHHHH
EAQLAAMVRNSRCDGFVLTPPLTDNVVLLDFLDRSGIRYVRIAPDIQPDRSPGVCIDDAA
HHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCEECHHH
AAAAAARHLWELGHRRFAVVRGPASHGAAGRRRQGFIDELHRLGAENPIIEAEGNFSFES
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCHHHHHHHCCCCCCEEECCCCCCHHC
GIAAGAKVLAATPRPTAIFAANDDSAAGVMVACSQAGLKVPNDVSVCGFDDSWVAKSVWP
CHHCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHH
YLTTVYQPIEEMGRAAAALLLRRDEPDNVLHELDFSLVVRASTAPPPQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]