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The map label for this gene is ald [H]

Identifier: 87199699

GI number: 87199699

Start: 1766893

End: 1768008

Strand: Reverse

Name: ald [H]

Synonym: Saro_1682

Alternate gene names: 87199699

Gene position: 1768008-1766893 (Counterclockwise)

Preceding gene: 87199701

Following gene: 87199695

Centisome position: 49.64

GC content: 67.29

Gene sequence:

>1116_bases
ATGCGCGTCGGAACCGTCCGTGAAATCAAGAACCATGAATACCGCGTCGGGCTTACCCCCGAAAGCGTCCGCGAACTCGT
CGCCCATGGCCACGAAGTGTGGGTCGAAGCCGGCGCGGGCAACGGCATAGGAGCAAGCGACGCCGACTACGAAGGCGCGG
GCGCGATCATCAAGGCCGATGCCAGGACCGTGTTCGACGGTTGCGAGATGATCGTGAAGGTCAAGGAACCCCAGGCCGTC
GAGCGCGCGATGCTGCGCCGTGGCCAGGTGCTCTACACTTATCTCCATCTCGCACCCGATCCGGAACAGACCGCCGACCT
CGTCAAGTCCGGCGTCACCGCGATCGCCTATGAAACCGTCACCGGCGCGGGCAATGCCCTGCCCCTGCTCAAGCCCATGA
GCCAGGTCGCAGGTCGCATGTCGATCCAGGCTGGCGCGACAGCGCTCGAAAAGGCCAACGGGGGCCGGGGCGTGCTGCTT
GGCGGCGTACCGGGCGTGCTGCCGGGCAAGGTCGTCGTCATCGGCGGCGGCGTGGTGGGGTTCAATGCGGCGCAGATGGC
CGTGGGGCTTGGCGCCGACGTGACCATCCTCGACCGCGACCCTGACGTGCTCGAACGCCTCGACAACCACTTCGAAGGCC
GCGCCCGCACGCTCTATTCGGGCAAGGCCGCGCTTGCAGCGCTGGTCGCCGAGGCCGACCTCGTGATCGGCGCCGTGCTG
ATCCCCGGCGCCGCCGCGCCGAAGCTGGTCACGCGCGACATGCTGGCGACGATGAAGCCCGGCGCGGTTCTCGTCGACGT
TGCCATCGACCAGGGCGGCTGCTTCGAGACCAGCAAGGCGACGACCCATGCCGACCCCACCTATGTCGTCGACGGCGTGG
TCCACTACTGCGTCGCCAACATGCCGGGCGCGGTTGCACGTACCAGCACCTACGCGCTGAACAACGTCACGCTCCCCCAC
GCGCTGCGCATCGCGGACAAGGGCTGGAAGGAAGCGCTGCGCGCCGACCCCAACCTCGCAGCGGGCCTGAACGTCCACGA
GGGCAAGGTCACTTACGAAGCCGTGGCAACCGAACTGGGATACGAATACACCCCGGTCGCCGACATTCTCGCCTGA

Upstream 100 bases:

>100_bases
CCAAACAGCCACATCGCAAGGCTGCAAATAGGCAAGAACTGCCGGGACCATCGCGATAGTCTTGCGTTTACCAGGAGACC
ACGAGCCACAGGAGCTTGTC

Downstream 100 bases:

>100_bases
CCGGGACCGGGGCGGCCTCCTTTAGAAGGTCGCCCCTCCCCGACTACCGCCCGTGCCTAGATCGCGTTGATGTCGACGAC
GGTCGGGATCGTGTCGTTTA

Product: L-alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRVGTVREIKNHEYRVGLTPESVRELVAHGHEVWVEAGAGNGIGASDADYEGAGAIIKADARTVFDGCEMIVKVKEPQAV
ERAMLRRGQVLYTYLHLAPDPEQTADLVKSGVTAIAYETVTGAGNALPLLKPMSQVAGRMSIQAGATALEKANGGRGVLL
GGVPGVLPGKVVVIGGGVVGFNAAQMAVGLGADVTILDRDPDVLERLDNHFEGRARTLYSGKAALAALVAEADLVIGAVL
IPGAAAPKLVTRDMLATMKPGAVLVDVAIDQGGCFETSKATTHADPTYVVDGVVHYCVANMPGAVARTSTYALNNVTLPH
ALRIADKGWKEALRADPNLAAGLNVHEGKVTYEAVATELGYEYTPVADILA

Sequences:

>Translated_371_residues
MRVGTVREIKNHEYRVGLTPESVRELVAHGHEVWVEAGAGNGIGASDADYEGAGAIIKADARTVFDGCEMIVKVKEPQAV
ERAMLRRGQVLYTYLHLAPDPEQTADLVKSGVTAIAYETVTGAGNALPLLKPMSQVAGRMSIQAGATALEKANGGRGVLL
GGVPGVLPGKVVVIGGGVVGFNAAQMAVGLGADVTILDRDPDVLERLDNHFEGRARTLYSGKAALAALVAEADLVIGAVL
IPGAAAPKLVTRDMLATMKPGAVLVDVAIDQGGCFETSKATTHADPTYVVDGVVHYCVANMPGAVARTSTYALNNVTLPH
ALRIADKGWKEALRADPNLAAGLNVHEGKVTYEAVATELGYEYTPVADILA
>Mature_371_residues
MRVGTVREIKNHEYRVGLTPESVRELVAHGHEVWVEAGAGNGIGASDADYEGAGAIIKADARTVFDGCEMIVKVKEPQAV
ERAMLRRGQVLYTYLHLAPDPEQTADLVKSGVTAIAYETVTGAGNALPLLKPMSQVAGRMSIQAGATALEKANGGRGVLL
GGVPGVLPGKVVVIGGGVVGFNAAQMAVGLGADVTILDRDPDVLERLDNHFEGRARTLYSGKAALAALVAEADLVIGAVL
IPGAAAPKLVTRDMLATMKPGAVLVDVAIDQGGCFETSKATTHADPTYVVDGVVHYCVANMPGAVARTSTYALNNVTLPH
ALRIADKGWKEALRADPNLAAGLNVHEGKVTYEAVATELGYEYTPVADILA

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=346, Percent_Identity=30.0578034682081, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI122939153, Length=346, Percent_Identity=30.0578034682081, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1787887, Length=346, Percent_Identity=33.2369942196532, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550456, Length=337, Percent_Identity=29.673590504451, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38633; Mature: 38633

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGTVREIKNHEYRVGLTPESVRELVAHGHEVWVEAGAGNGIGASDADYEGAGAIIKAD
CCCCCHHHHCCCCEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEECC
ARTVFDGCEMIVKVKEPQAVERAMLRRGQVLYTYLHLAPDPEQTADLVKSGVTAIAYETV
CHHHHHHCEEEEEECCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEEE
TGAGNALPLLKPMSQVAGRMSIQAGATALEKANGGRGVLLGGVPGVLPGKVVVIGGGVVG
CCCCCCCHHHHHHHHHHHCEEHHHCCHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
FNAAQMAVGLGADVTILDRDPDVLERLDNHFEGRARTLYSGKAALAALVAEADLVIGAVL
CCHHHHHEECCCCEEEECCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHEEHEE
IPGAAAPKLVTRDMLATMKPGAVLVDVAIDQGGCFETSKATTHADPTYVVDGVVHYCVAN
CCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
MPGAVARTSTYALNNVTLPHALRIADKGWKEALRADPNLAAGLNVHEGKVTYEAVATELG
CCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCEECCCCCHHHHHHHHHC
YEYTPVADILA
CCCCCHHHHCC
>Mature Secondary Structure
MRVGTVREIKNHEYRVGLTPESVRELVAHGHEVWVEAGAGNGIGASDADYEGAGAIIKAD
CCCCCHHHHCCCCEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEECC
ARTVFDGCEMIVKVKEPQAVERAMLRRGQVLYTYLHLAPDPEQTADLVKSGVTAIAYETV
CHHHHHHCEEEEEECCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEEE
TGAGNALPLLKPMSQVAGRMSIQAGATALEKANGGRGVLLGGVPGVLPGKVVVIGGGVVG
CCCCCCCHHHHHHHHHHHCEEHHHCCHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
FNAAQMAVGLGADVTILDRDPDVLERLDNHFEGRARTLYSGKAALAALVAEADLVIGAVL
CCHHHHHEECCCCEEEECCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHEEHEE
IPGAAAPKLVTRDMLATMKPGAVLVDVAIDQGGCFETSKATTHADPTYVVDGVVHYCVAN
CCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
MPGAVARTSTYALNNVTLPHALRIADKGWKEALRADPNLAAGLNVHEGKVTYEAVATELG
CCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCEECCCCCHHHHHHHHHC
YEYTPVADILA
CCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]