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The map label for this gene is glgB

Identifier: 87199675

GI number: 87199675

Start: 1735553

End: 1737718

Strand: Reverse

Name: glgB

Synonym: Saro_1658

Alternate gene names: 87199675

Gene position: 1737718-1735553 (Counterclockwise)

Preceding gene: 87199676

Following gene: 87199674

Centisome position: 48.79

GC content: 65.93

Gene sequence:

>2166_bases
ATGAAACCACCGGCGAGCGCCATCGATGCCCTTCTGGACGGGACGCACGCCGATCCTTTCTCGCTTCTCGGCATCCACGA
AGGACCGGACGGCGCCTTCGCCCGCGCCGTCCTGCCCGGCGCGGAAGAGGCCGTGGCATGGTCGCTCTCGGGCAAGAAGC
TGGGCAAGCTCAGCCGCGTCGACGGGCGCGGCCTGTTCGAAGGCAAGGTAAAGGGACCGCGCCAGCCAGTCCGCTATGCC
TGCAAGGCCGATGGGCACGAATGGCTCGTCACCGACCCCTATTCCTTCGGCCCCGTCCTCGGCCCGCTCGACGATTTCCT
GATCGCCGAGGGCACGCATCTGCGGCTGTTCGACAAGATGGGTGCGCACCTGATCGAGCACGAGGGCGCGCGCGGCGTGC
ACTTCGCGGTTTGGGCGCCCAACGCGCGGCTCGTCTCGGTCGTCGGTGATTTCAACGACTGGGATCACCGCCGCCACCCG
ATGCGCCGCCGGGCGGACATCGGCGTCTGGGAAATCTTCATTCCCGACATCGGCGAACATCGCGCCTACAAGTACCGCAT
CGTCGGCCACGACGGCGGTGTGCTTCCGCTCAAAGCCGATCCCTATGCGCTTGCCGCCGAATTCCGCCCCAGCACCGCCT
CGCTCACCGCGCACCCGGTCAAGATGGACTGGGCCGACGCCGCCCACCGCGCGCACTGGGCCTCGGTCGACGCGCGCCGC
GAGCCGATGTCGATCTACGAGGTCCACCCCGGCTCGTGGCAGAAGCCGCACGAGGAAGGCTTCCATACCTGGGATGAGTT
GGCCGACCGCCTGATCCCCTATGTCGCGGAAATGGGCTTCACCCACATTGAATTCCTGCCCGTCTCCGAGCACCCCTACG
ATCCGAGCTGGGGCTACCAGACGACCGGCCTCTACGCCCCGTCAGCCCGCTTCGGCCCGCCGGAGGGGTTCGCCCGTTTC
GTCGATGGCGCCCACCGGGCGGGCATTTCCGTCCTTATCGACTGGGTCCCTGCCCACTTCCCGACGGACGAGCACGGCCT
CGTCCGTTTTGACGGCACAGCGCTCTACGAACACGAAGACCCTCGCCTCGGCTTCCATCCCGACTGGAACACGCTGATCT
ACAACTTCGGCCGCCGCGAGGTCGTCAGCTTCCTCGTCAACAACGCGCTGTTCTGGGCCGAACGCTATCATGTCGACGGG
CTGCGCGTGGATGCGGTCGCCTCGATGCTGTACCGCGACTACTCGCGCAAGGCTGGCGAGTGGATTCCCAACGCCGAAGG
CGGCCGCGAGAACTGGGAAGCGGTCGAGTTCCTCAAGGCGATGAACCGGGCCGTCTATGGCAGCCACGCCGGCTTCCTCA
CCATCGCCGAGGAATCAACCGCGTGGCCCGGCGTATCCAAACCGGCGTTCGATGGCGCCCCGCGCGAGAACCTCGGCTTC
GGCTTCAAGTGGAACATGGGCTTCATGCACGATACGCTGAAGTACATGGCGCGCGAGCCGATCCACCGCCGCTACCATCA
CGATGAGATCACCTTCGGCCTGATGTACGCCTTCAGCGAGAACTTCGTCCTGCCGCTGAGCCATGACGAAGTGGTCCACG
GCAAGGGCAGCCTGCTCAACAAGATGAGCGGCGATGACTGGCAGAAGTTCGCCAACCTGCGCGCCTATTACGGGCTGATG
TGGGGCTATCCCGGCAAGAAGCTGCTGTTCATGGGCCAGGAATTCGCCCAGCGCCGCGAGTGGAGCGAGGCTCGCGCGCT
CGACTGGGACCTGCTCCAGGCTCCCGCCCACGAGGGCATCCGCCGCTGGGTACGCGATCTCAACCGCGTCTATGCCAGCC
GCCCCGCACTCCACGCCCGCGATTGCGAGCCGGAAGGGTTCGAATGGCTGGTGGTGGACGATGCGGAAGCCTCGATCTTC
GCCTGGCTGCGCAAGGCACCGGGCGCAAGGCCGGTCGCTGTGATCTGCAACATGACCCCGCAGGTCCACGACCACTATCG
CCTGCCGCTTCCGCTCGATGGGGAATGGCGCGAGGTCCTGAACAGCGATGCCGAGGACTATGGCGGCAGCGGCATCGGCA
ATCTTGGCAAGGTCACTGCCGAACAAGGCGCCGCTTTCGTGGTCCTCCCGCCGCTGGCCACGCTGATGCTTGAATTCGAA
GGATAA

Upstream 100 bases:

>100_bases
GCGCTTCGGCAATCCGCAACATTGCCAACGTAGGCTGGTTCTCGTCCGATCGCACGATCGGCGAATATGCCCGCGACATC
TGGGGGGTCCGAGACCGAGC

Downstream 100 bases:

>100_bases
GAAGGAAGGGGAAGAACGGGGATGCGCGATACCTACTCTCAGCCGCTGGCGCGCGATGCGATGGCCTATGTCCTGGCCGG
CGGACGCGGCAGCCGCCTCA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MKPPASAIDALLDGTHADPFSLLGIHEGPDGAFARAVLPGAEEAVAWSLSGKKLGKLSRVDGRGLFEGKVKGPRQPVRYA
CKADGHEWLVTDPYSFGPVLGPLDDFLIAEGTHLRLFDKMGAHLIEHEGARGVHFAVWAPNARLVSVVGDFNDWDHRRHP
MRRRADIGVWEIFIPDIGEHRAYKYRIVGHDGGVLPLKADPYALAAEFRPSTASLTAHPVKMDWADAAHRAHWASVDARR
EPMSIYEVHPGSWQKPHEEGFHTWDELADRLIPYVAEMGFTHIEFLPVSEHPYDPSWGYQTTGLYAPSARFGPPEGFARF
VDGAHRAGISVLIDWVPAHFPTDEHGLVRFDGTALYEHEDPRLGFHPDWNTLIYNFGRREVVSFLVNNALFWAERYHVDG
LRVDAVASMLYRDYSRKAGEWIPNAEGGRENWEAVEFLKAMNRAVYGSHAGFLTIAEESTAWPGVSKPAFDGAPRENLGF
GFKWNMGFMHDTLKYMAREPIHRRYHHDEITFGLMYAFSENFVLPLSHDEVVHGKGSLLNKMSGDDWQKFANLRAYYGLM
WGYPGKKLLFMGQEFAQRREWSEARALDWDLLQAPAHEGIRRWVRDLNRVYASRPALHARDCEPEGFEWLVVDDAEASIF
AWLRKAPGARPVAVICNMTPQVHDHYRLPLPLDGEWREVLNSDAEDYGGSGIGNLGKVTAEQGAAFVVLPPLATLMLEFE
G

Sequences:

>Translated_721_residues
MKPPASAIDALLDGTHADPFSLLGIHEGPDGAFARAVLPGAEEAVAWSLSGKKLGKLSRVDGRGLFEGKVKGPRQPVRYA
CKADGHEWLVTDPYSFGPVLGPLDDFLIAEGTHLRLFDKMGAHLIEHEGARGVHFAVWAPNARLVSVVGDFNDWDHRRHP
MRRRADIGVWEIFIPDIGEHRAYKYRIVGHDGGVLPLKADPYALAAEFRPSTASLTAHPVKMDWADAAHRAHWASVDARR
EPMSIYEVHPGSWQKPHEEGFHTWDELADRLIPYVAEMGFTHIEFLPVSEHPYDPSWGYQTTGLYAPSARFGPPEGFARF
VDGAHRAGISVLIDWVPAHFPTDEHGLVRFDGTALYEHEDPRLGFHPDWNTLIYNFGRREVVSFLVNNALFWAERYHVDG
LRVDAVASMLYRDYSRKAGEWIPNAEGGRENWEAVEFLKAMNRAVYGSHAGFLTIAEESTAWPGVSKPAFDGAPRENLGF
GFKWNMGFMHDTLKYMAREPIHRRYHHDEITFGLMYAFSENFVLPLSHDEVVHGKGSLLNKMSGDDWQKFANLRAYYGLM
WGYPGKKLLFMGQEFAQRREWSEARALDWDLLQAPAHEGIRRWVRDLNRVYASRPALHARDCEPEGFEWLVVDDAEASIF
AWLRKAPGARPVAVICNMTPQVHDHYRLPLPLDGEWREVLNSDAEDYGGSGIGNLGKVTAEQGAAFVVLPPLATLMLEFE
G
>Mature_721_residues
MKPPASAIDALLDGTHADPFSLLGIHEGPDGAFARAVLPGAEEAVAWSLSGKKLGKLSRVDGRGLFEGKVKGPRQPVRYA
CKADGHEWLVTDPYSFGPVLGPLDDFLIAEGTHLRLFDKMGAHLIEHEGARGVHFAVWAPNARLVSVVGDFNDWDHRRHP
MRRRADIGVWEIFIPDIGEHRAYKYRIVGHDGGVLPLKADPYALAAEFRPSTASLTAHPVKMDWADAAHRAHWASVDARR
EPMSIYEVHPGSWQKPHEEGFHTWDELADRLIPYVAEMGFTHIEFLPVSEHPYDPSWGYQTTGLYAPSARFGPPEGFARF
VDGAHRAGISVLIDWVPAHFPTDEHGLVRFDGTALYEHEDPRLGFHPDWNTLIYNFGRREVVSFLVNNALFWAERYHVDG
LRVDAVASMLYRDYSRKAGEWIPNAEGGRENWEAVEFLKAMNRAVYGSHAGFLTIAEESTAWPGVSKPAFDGAPRENLGF
GFKWNMGFMHDTLKYMAREPIHRRYHHDEITFGLMYAFSENFVLPLSHDEVVHGKGSLLNKMSGDDWQKFANLRAYYGLM
WGYPGKKLLFMGQEFAQRREWSEARALDWDLLQAPAHEGIRRWVRDLNRVYASRPALHARDCEPEGFEWLVVDDAEASIF
AWLRKAPGARPVAVICNMTPQVHDHYRLPLPLDGEWREVLNSDAEDYGGSGIGNLGKVTAEQGAAFVVLPPLATLMLEFE
G

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=612, Percent_Identity=26.4705882352941, Blast_Score=160, Evalue=5e-39,
Organism=Escherichia coli, GI1789839, Length=725, Percent_Identity=52.9655172413793, Blast_Score=744, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=416, Percent_Identity=27.8846153846154, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI32564391, Length=309, Percent_Identity=30.0970873786408, Blast_Score=135, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6320826, Length=392, Percent_Identity=28.0612244897959, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28573410, Length=614, Percent_Identity=27.1986970684039, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_NOVAD (Q2G7S5)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496932.1
- ProteinModelPortal:   Q2G7S5
- SMR:   Q2G7S5
- STRING:   Q2G7S5
- GeneID:   3918767
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1658
- NMPDR:   fig|48935.1.peg.1329
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   PPLATIM
- PhylomeDB:   Q2G7S5
- ProtClustDB:   PRK05402
- BioCyc:   NARO279238:SARO_1658-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 81220; Mature: 81220

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: ACT_SITE 299-299 ACT_SITE 334-334 ACT_SITE 339-339 ACT_SITE 402-402 ACT_SITE 404-404 ACT_SITE 457-457 ACT_SITE 528-528 ACT_SITE 529-529

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPASAIDALLDGTHADPFSLLGIHEGPDGAFARAVLPGAEEAVAWSLSGKKLGKLSRV
CCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHEEEECCCCHHCCCCCC
DGRGLFEGKVKGPRQPVRYACKADGHEWLVTDPYSFGPVLGPLDDFLIAEGTHLRLFDKM
CCCCEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCEEEHHHHH
GAHLIEHEGARGVHFAVWAPNARLVSVVGDFNDWDHRRHPMRRRADIGVWEIFIPDIGEH
CHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCHHHCCHHHHHCCCCEEEEECCCCCCC
RAYKYRIVGHDGGVLPLKADPYALAAEFRPSTASLTAHPVKMDWADAAHRAHWASVDARR
CEEEEEEEECCCCEEEECCCCCEEEEECCCCCCCEEECCEEECHHHHHHHHHHHCCCCCC
EPMSIYEVHPGSWQKPHEEGFHTWDELADRLIPYVAEMGFTHIEFLPVSEHPYDPSWGYQ
CCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
TTGLYAPSARFGPPEGFARFVDGAHRAGISVLIDWVPAHFPTDEHGLVRFDGTALYEHED
CCCEECCCCCCCCHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCEEEECCEEEEECCC
PRLGFHPDWNTLIYNFGRREVVSFLVNNALFWAERYHVDGLRVDAVASMLYRDYSRKAGE
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHHCCC
WIPNAEGGRENWEAVEFLKAMNRAVYGSHAGFLTIAEESTAWPGVSKPAFDGAPRENLGF
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GFKWNMGFMHDTLKYMAREPIHRRYHHDEITFGLMYAFSENFVLPLSHDEVVHGKGSLLN
CEEECCCHHHHHHHHHHHCHHHHHHCCCCCEEEEEEEECCCEEEECCCCCEECCCCHHHH
KMSGDDWQKFANLRAYYGLMWGYPGKKLLFMGQEFAQRREWSEARALDWDLLQAPAHEGI
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RRWVRDLNRVYASRPALHARDCEPEGFEWLVVDDAEASIFAWLRKAPGARPVAVICNMTP
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCC
QVHDHYRLPLPLDGEWREVLNSDAEDYGGSGIGNLGKVTAEQGAAFVVLPPLATLMLEFE
CCCCCEECCCCCCCHHHHHHCCCCHHCCCCCCCCCCCCHHHCCCEEEEECCCEEEEEEEC
G
C
>Mature Secondary Structure
MKPPASAIDALLDGTHADPFSLLGIHEGPDGAFARAVLPGAEEAVAWSLSGKKLGKLSRV
CCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHEEEECCCCHHCCCCCC
DGRGLFEGKVKGPRQPVRYACKADGHEWLVTDPYSFGPVLGPLDDFLIAEGTHLRLFDKM
CCCCEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCEEEHHHHH
GAHLIEHEGARGVHFAVWAPNARLVSVVGDFNDWDHRRHPMRRRADIGVWEIFIPDIGEH
CHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCHHHCCHHHHHCCCCEEEEECCCCCCC
RAYKYRIVGHDGGVLPLKADPYALAAEFRPSTASLTAHPVKMDWADAAHRAHWASVDARR
CEEEEEEEECCCCEEEECCCCCEEEEECCCCCCCEEECCEEECHHHHHHHHHHHCCCCCC
EPMSIYEVHPGSWQKPHEEGFHTWDELADRLIPYVAEMGFTHIEFLPVSEHPYDPSWGYQ
CCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
TTGLYAPSARFGPPEGFARFVDGAHRAGISVLIDWVPAHFPTDEHGLVRFDGTALYEHED
CCCEECCCCCCCCHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCEEEECCEEEEECCC
PRLGFHPDWNTLIYNFGRREVVSFLVNNALFWAERYHVDGLRVDAVASMLYRDYSRKAGE
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHHCCC
WIPNAEGGRENWEAVEFLKAMNRAVYGSHAGFLTIAEESTAWPGVSKPAFDGAPRENLGF
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GFKWNMGFMHDTLKYMAREPIHRRYHHDEITFGLMYAFSENFVLPLSHDEVVHGKGSLLN
CEEECCCHHHHHHHHHHHCHHHHHHCCCCCEEEEEEEECCCEEEECCCCCEECCCCHHHH
KMSGDDWQKFANLRAYYGLMWGYPGKKLLFMGQEFAQRREWSEARALDWDLLQAPAHEGI
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RRWVRDLNRVYASRPALHARDCEPEGFEWLVVDDAEASIFAWLRKAPGARPVAVICNMTP
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCC
QVHDHYRLPLPLDGEWREVLNSDAEDYGGSGIGNLGKVTAEQGAAFVVLPPLATLMLEFE
CCCCCEECCCCCCCHHHHHHCCCCHHCCCCCCCCCCCCHHHCCCEEEEECCCEEEEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA