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The map label for this gene is pspA [H]

Identifier: 87199178

GI number: 87199178

Start: 1203995

End: 1204819

Strand: Direct

Name: pspA [H]

Synonym: Saro_1156

Alternate gene names: 87199178

Gene position: 1203995-1204819 (Clockwise)

Preceding gene: 87199177

Following gene: 87199179

Centisome position: 33.81

GC content: 65.21

Gene sequence:

>825_bases
ATGGCTGACGACCTCTCTCCGATCCGCCCGGAACGAGCCGATGGTGGTCGCACCACCCGGCTCGACGCCGAGCTTGATCG
GCTGCGCTCTTCGACTGCAAATGGTGCGTCCGGCCAGAACCGGCCCGCTTTCACTTCCGGAGTAAACCTGATGGGCATCT
TTTCGCGTACCCGCGATATCATTGCCGCCAATTTCAATGACTTGCTCGACAAGGCCGATGATCCGGCGAAGATGATCCGG
ATGATCATCATGGAGATGGAGGAGACGCTGGTCGAAGTCCGTGCCTCGGCGGCGCGCACCATCGCCGACCAGAAGGAAAT
GGGCCGCCACGTGTCCCGGCTGGAACGCCTCCAGGCCGACTGGTCCGAAAAGGCCCAGCTCGCCCTGTCGAAGGACCGCG
AGGACCTTGCCCGCGCCGCTCTCGTCGAAAAGAAGAAAGCCGCCGACATGGCCGACCAGCTCAAGGTCGAGATTGCCGTC
CTCGACGATGCGATGCGCGCCTACGAGCAGGACATCGAAAAGCTCCAGACCCGCCTGCGCGAGGCCCGCAGCCGCCAGAC
CGCCATCGCCGCGCGCCTCGAAAGCGCCGAGAACCGCGTCCGCCTGCGCAGCTTGCTTGCCAGCGAGCGTGTCGATGAAG
CGATGGCCCGTTTCGACCAGCTCGAACGCCGCGTCGATTATGCCGAAGGCCGCGCCGACGCGCTCAGCCTCGCCGACGGC
AGCGCCAAGCCCAGCCTCGCGGACGAGATCGCCGCGCTCGCCGGGCAGGACAAGATCGACGAGGAACTCGAAGCGATGAA
GCGCCAGCTCGGAAAGGAAGCCTGA

Upstream 100 bases:

>100_bases
GTGCGATGGTGGTGATGTGCTGCTTTGCCCTGACCCAGCAGGTCCGCGCCGTGCCGACCGTCATGGCATCCTCGGCCGCC
ATTACCGGCGAGCTGGCATG

Downstream 100 bases:

>100_bases
TCCATGGAAGACGTTGTTGCGATCGCCGCGATTTTTCTCGGCCTGCCGTGGATCATCTTCCACTACATCACCAAGTGGAA
GACCGCCGCCACCCTGACGA

Product: phage shock protein A, PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MADDLSPIRPERADGGRTTRLDAELDRLRSSTANGASGQNRPAFTSGVNLMGIFSRTRDIIAANFNDLLDKADDPAKMIR
MIIMEMEETLVEVRASAARTIADQKEMGRHVSRLERLQADWSEKAQLALSKDREDLARAALVEKKKAADMADQLKVEIAV
LDDAMRAYEQDIEKLQTRLREARSRQTAIAARLESAENRVRLRSLLASERVDEAMARFDQLERRVDYAEGRADALSLADG
SAKPSLADEIAALAGQDKIDEELEAMKRQLGKEA

Sequences:

>Translated_274_residues
MADDLSPIRPERADGGRTTRLDAELDRLRSSTANGASGQNRPAFTSGVNLMGIFSRTRDIIAANFNDLLDKADDPAKMIR
MIIMEMEETLVEVRASAARTIADQKEMGRHVSRLERLQADWSEKAQLALSKDREDLARAALVEKKKAADMADQLKVEIAV
LDDAMRAYEQDIEKLQTRLREARSRQTAIAARLESAENRVRLRSLLASERVDEAMARFDQLERRVDYAEGRADALSLADG
SAKPSLADEIAALAGQDKIDEELEAMKRQLGKEA
>Mature_273_residues
ADDLSPIRPERADGGRTTRLDAELDRLRSSTANGASGQNRPAFTSGVNLMGIFSRTRDIIAANFNDLLDKADDPAKMIRM
IIMEMEETLVEVRASAARTIADQKEMGRHVSRLERLQADWSEKAQLALSKDREDLARAALVEKKKAADMADQLKVEIAVL
DDAMRAYEQDIEKLQTRLREARSRQTAIAARLESAENRVRLRSLLASERVDEAMARFDQLERRVDYAEGRADALSLADGS
AKPSLADEIAALAGQDKIDEELEAMKRQLGKEA

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=217, Percent_Identity=44.2396313364055, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 30497; Mature: 30366

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDLSPIRPERADGGRTTRLDAELDRLRSSTANGASGQNRPAFTSGVNLMGIFSRTRDI
CCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
IAANFNDLLDKADDPAKMIRMIIMEMEETLVEVRASAARTIADQKEMGRHVSRLERLQAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WSEKAQLALSKDREDLARAALVEKKKAADMADQLKVEIAVLDDAMRAYEQDIEKLQTRLR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARSRQTAIAARLESAENRVRLRSLLASERVDEAMARFDQLERRVDYAEGRADALSLADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
SAKPSLADEIAALAGQDKIDEELEAMKRQLGKEA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADDLSPIRPERADGGRTTRLDAELDRLRSSTANGASGQNRPAFTSGVNLMGIFSRTRDI
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
IAANFNDLLDKADDPAKMIRMIIMEMEETLVEVRASAARTIADQKEMGRHVSRLERLQAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WSEKAQLALSKDREDLARAALVEKKKAADMADQLKVEIAVLDDAMRAYEQDIEKLQTRLR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARSRQTAIAARLESAENRVRLRSLLASERVDEAMARFDQLERRVDYAEGRADALSLADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
SAKPSLADEIAALAGQDKIDEELEAMKRQLGKEA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]