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The map label for this gene is pheS

Identifier: 87198712

GI number: 87198712

Start: 725769

End: 726869

Strand: Direct

Name: pheS

Synonym: Saro_0688

Alternate gene names: 87198712

Gene position: 725769-726869 (Clockwise)

Preceding gene: 87198711

Following gene: 87198713

Centisome position: 20.38

GC content: 65.03

Gene sequence:

>1101_bases
ATGGAACAGCTCGATCAACAGCAGGCCGAAACGCTTTCGGCCATCGCGCAAGCGGCCACGCCCGAAGCAGTGGAGGCGAT
CCGCGTCTCGGCGCTCGGCAAGCAGGGGTGGGTCAGCGCCCTGCTCAAGTCGCTGGGCGGAATGACCCCGGAACAGCGCC
AGAGCGAAGGCCCGAAAATCCACGCCGCGCGTGAGGCCGTGACAACAGCCTTGGCCGAACGCAAGTCTGCGCTCGAAGGG
GCAGCGCTCGAAGCGCGCCTCGCTGCCGAAACGGTGGACCTCTCGCTCCCCGCGCCCGACCTGGCGAAAGGGTCGGTCCA
CCCCGTGTCGCAGGTCATGGACGAGCTGGCCGAGATCTTCGCGGACATGGGCTTCGCCGTGGCCAGCGGGCCGGAGATCG
AGGACGACTGGCACAACTTCACCGCGCTCAACATGCCGGAAACCCACCCGGCGCGGGCGATGCACGATACCTTCTACTTC
CCCGACAAGGACGCGGAAGGCCGCTCGATGCTGTTGCGCACGCACACCTCGCCGGTGCAGATCCGCTCGATGCTGAAAGC
GGGCGCGCCCTTGCGCATCATTGCGCCCGGCCGTGTCTACCGCTCGGATTCCGACGCGACGCACACGCCGATGTTCCACC
AGATCGAAGGTCTCGTCATCGACAAGGGCATTCACCTCGGTCACCTCAAGTGGACGCTGGAGACCTTCCTCAAGGCCTTC
TTCGAACGTGACGACATCGTCCTGCGCCTGCGACCCAGCTACTTCCCCTTCACCGAACCATCGGTCGAAGTCGACGTCGG
CTACACACTGGTGAACGGAAAGCGAGTCGTCGGCGGCAGTGGCGATGCGGACAACGGCGGCTGGATGGAAGTTCTGGGCT
CCGGCATGGTCAACCGCAAGGTCATCGAATTCGGCGGCCTCGATCCCGATGAATGGCAGGGCTTCGCTTTCGGCACCGGT
GTCGATCGCCTTGCCATGCTCAAGTACGGCATGGACGACCTTCGCGCCTTCTTCGATGGCGATGCGCGGTGGCTCGGCCA
CTACGGTTTCGGCGCACTCGACGTGCCGACCCTTTCCGGCGGTGTTGGAGTACGCTCGTGA

Upstream 100 bases:

>100_bases
GCAGGCGTTCGTCGGCGCGGCGGTCCAGGCGCTCCATGCGCCGGGGCGCGGATAAGTCCACACTTTGATTCTCTCGCGTT
TGCGGCTAGCGGCTGCGCGC

Downstream 100 bases:

>100_bases
AGTTCTCGTTCTCGTGGTTGAAGTCGGTCCTCGATACCAAGGCCGATGCGAAGCTGATCGCGGAAAAGCTGACCTCGCTG
GGTCTGGAGATCGAAAGCGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MEQLDQQQAETLSAIAQAATPEAVEAIRVSALGKQGWVSALLKSLGGMTPEQRQSEGPKIHAAREAVTTALAERKSALEG
AALEARLAAETVDLSLPAPDLAKGSVHPVSQVMDELAEIFADMGFAVASGPEIEDDWHNFTALNMPETHPARAMHDTFYF
PDKDAEGRSMLLRTHTSPVQIRSMLKAGAPLRIIAPGRVYRSDSDATHTPMFHQIEGLVIDKGIHLGHLKWTLETFLKAF
FERDDIVLRLRPSYFPFTEPSVEVDVGYTLVNGKRVVGGSGDADNGGWMEVLGSGMVNRKVIEFGGLDPDEWQGFAFGTG
VDRLAMLKYGMDDLRAFFDGDARWLGHYGFGALDVPTLSGGVGVRS

Sequences:

>Translated_366_residues
MEQLDQQQAETLSAIAQAATPEAVEAIRVSALGKQGWVSALLKSLGGMTPEQRQSEGPKIHAAREAVTTALAERKSALEG
AALEARLAAETVDLSLPAPDLAKGSVHPVSQVMDELAEIFADMGFAVASGPEIEDDWHNFTALNMPETHPARAMHDTFYF
PDKDAEGRSMLLRTHTSPVQIRSMLKAGAPLRIIAPGRVYRSDSDATHTPMFHQIEGLVIDKGIHLGHLKWTLETFLKAF
FERDDIVLRLRPSYFPFTEPSVEVDVGYTLVNGKRVVGGSGDADNGGWMEVLGSGMVNRKVIEFGGLDPDEWQGFAFGTG
VDRLAMLKYGMDDLRAFFDGDARWLGHYGFGALDVPTLSGGVGVRS
>Mature_366_residues
MEQLDQQQAETLSAIAQAATPEAVEAIRVSALGKQGWVSALLKSLGGMTPEQRQSEGPKIHAAREAVTTALAERKSALEG
AALEARLAAETVDLSLPAPDLAKGSVHPVSQVMDELAEIFADMGFAVASGPEIEDDWHNFTALNMPETHPARAMHDTFYF
PDKDAEGRSMLLRTHTSPVQIRSMLKAGAPLRIIAPGRVYRSDSDATHTPMFHQIEGLVIDKGIHLGHLKWTLETFLKAF
FERDDIVLRLRPSYFPFTEPSVEVDVGYTLVNGKRVVGGSGDADNGGWMEVLGSGMVNRKVIEFGGLDPDEWQGFAFGTG
VDRLAMLKYGMDDLRAFFDGDARWLGHYGFGALDVPTLSGGVGVRS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=273, Percent_Identity=32.6007326007326, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI5729820, Length=254, Percent_Identity=28.3464566929134, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788007, Length=348, Percent_Identity=50.5747126436782, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI32563657, Length=276, Percent_Identity=29.3478260869565, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=276, Percent_Identity=29.3478260869565, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=264, Percent_Identity=31.0606060606061, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=378, Percent_Identity=25.6613756613757, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=294, Percent_Identity=24.1496598639456, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18858079, Length=276, Percent_Identity=30.0724637681159, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_NOVAD (Q2GAI8)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495969.1
- ProteinModelPortal:   Q2GAI8
- SMR:   Q2GAI8
- STRING:   Q2GAI8
- GeneID:   3918113
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0688
- NMPDR:   fig|48935.1.peg.1927
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q2GAI8
- ProtClustDB:   PRK00488
- BioCyc:   NARO279238:SARO_0688-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39734; Mature: 39734

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQLDQQQAETLSAIAQAATPEAVEAIRVSALGKQGWVSALLKSLGGMTPEQRQSEGPKI
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHCCCCCC
HAAREAVTTALAERKSALEGAALEARLAAETVDLSLPAPDLAKGSVHPVSQVMDELAEIF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ADMGFAVASGPEIEDDWHNFTALNMPETHPARAMHDTFYFPDKDAEGRSMLLRTHTSPVQ
HHCCCEECCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCHHH
IRSMLKAGAPLRIIAPGRVYRSDSDATHTPMFHQIEGLVIDKGIHLGHLKWTLETFLKAF
HHHHHHCCCCEEEEECCCEECCCCCCCCCCHHHHHCCEEEECCCEEEHHHHHHHHHHHHH
FERDDIVLRLRPSYFPFTEPSVEVDVGYTLVNGKRVVGGSGDADNGGWMEVLGSGMVNRK
HCCCCEEEEECCCCCCCCCCCEEEEECEEEECCCEEECCCCCCCCCCHHHHHCCCCCCCE
VIEFGGLDPDEWQGFAFGTGVDRLAMLKYGMDDLRAFFDGDARWLGHYGFGALDVPTLSG
EEEECCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GVGVRS
CCCCCC
>Mature Secondary Structure
MEQLDQQQAETLSAIAQAATPEAVEAIRVSALGKQGWVSALLKSLGGMTPEQRQSEGPKI
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHCCCCCC
HAAREAVTTALAERKSALEGAALEARLAAETVDLSLPAPDLAKGSVHPVSQVMDELAEIF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
ADMGFAVASGPEIEDDWHNFTALNMPETHPARAMHDTFYFPDKDAEGRSMLLRTHTSPVQ
HHCCCEECCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCHHH
IRSMLKAGAPLRIIAPGRVYRSDSDATHTPMFHQIEGLVIDKGIHLGHLKWTLETFLKAF
HHHHHHCCCCEEEEECCCEECCCCCCCCCCHHHHHCCEEEECCCEEEHHHHHHHHHHHHH
FERDDIVLRLRPSYFPFTEPSVEVDVGYTLVNGKRVVGGSGDADNGGWMEVLGSGMVNRK
HCCCCEEEEECCCCCCCCCCCEEEEECEEEECCCEEECCCCCCCCCCHHHHHCCCCCCCE
VIEFGGLDPDEWQGFAFGTGVDRLAMLKYGMDDLRAFFDGDARWLGHYGFGALDVPTLSG
EEEECCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GVGVRS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA