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The map label for this gene is dnaG [H]

Identifier: 87198665

GI number: 87198665

Start: 679419

End: 681137

Strand: Direct

Name: dnaG [H]

Synonym: Saro_0641

Alternate gene names: 87198665

Gene position: 679419-681137 (Clockwise)

Preceding gene: 87198664

Following gene: 87198666

Centisome position: 19.08

GC content: 65.74

Gene sequence:

>1719_bases
ATGGCCATCCCGCATCAATTCCTGGACGAGCTTCGATCGCGCACGACACTGTCCGCGCTGATCGCGCAGACCCTGAAAAT
CGACAAGGCAGGTCGCGAGTTCAAGGCCTGTTGCCCGTTCCACGGCGAGAAGACGCCGAGCTTCACCATCAACGATGCCA
AGGGTTTCTATCATTGCTTCGGATGCGGCGCCCACGGCGATGCCATCCGGTGGATGATCGACCAGCGTGGCCTGCAGTTC
ATCGACGCTGTGAGCGAGCTGGCGAGCGCGGCGGGCATGAAAGTGCCGGCGCCGACGCCGCAGGAGCGCGAACGTTCTGC
CAAGATCGAGACGGTGCGCGAGGCTCTGGACATCGCGCAGGGCATCTTCGTGGGCCAACTGCGCGAGGCCGGTGCCGTGA
TGCAGTACCTCGAGCGGCGCGGCATGACGCCGATGGATCTGGAGATCTTCGGCATCGGCTACGCCCGTGGCATGGACGGA
TCATTGCGCGGGTCTGGCATCGGTCGGCAGATCGGGCAGGCTGCCGGCCTGTTGAGCCAGCGCGAAGATGGATCCTTGCG
CGAGATGTTCCACGATCGCGTCACGGTTCCGATCCACGATGCGCGCGGGCGCCTCGTCGGGTTCGGCGGTCGCGTCTGGC
CTGGTCGACGTCGCGACACGCCGAAGTTCGTGAATAGCCCCGATAGTCCGATCTTCGACAAGGGACGGACCTTGTTCAAT
CTGCATCGTGCGGCGCCAGCGGCGCGGCCGGGTGCAGAGAACCGGCTGATCGTCGTCGAAGGCTATTTCGACGTCGTGTC
CATGGCCAGGGCGGGCTTTGCCGCTTGTGTTGCGCCGATGGGTACCGCGCTGACGGAAGAGCAGCTGGTACGTTGCTGGC
GGCTTCATCACCGGCCGGTGCTGTTGTTCGATGGCGATTCCGCAGGGCAAAAGGCTGCGGTGCGGGCCTGCCGCACGGCC
ATCCCGGGTATCGGGCCCGGGCGCGAGCTGGCGATCGCATTGCTGCCTGACGGGAAGGACCCTGACGAGCTGCTGTACAT
GGACGCCACCGGCATGGGCCGGCGCGCCATCGAAGCGATCGTGACCGAAGCCCTTCCGCTGCATACGTTCCTGTTCGATG
CGATCGTCGCGGAGGCGGGCGCAAATCCGACGCCAGAGCAGACGGCCGCGGTGTGGGCAGAACTGGCCGCGCTGGCCGGG
GAGATCGCCGACGAGGAGACGCGGTGCCAGTACCTTGGCACATGGCGTGCCCGGTTCGAGCGCGAGATCTCTGCGCTGCC
GCAGGTCCACGAGGCAGAGCCGCTGCATGCGGTGGTCGCGGCCGAGGACGGCGAATACGTGTTTCCCGAAAGCGAGTCCG
ACAGCCAGCGGCGTTTGATCGCGCTGGTGCGTGCGGTGCTCAAGAAGCGCGAGGAACGGCGCGAGATCAACGAAGAGATC
GCGGACCTGATGAAGATGGCCGAACTCGCCGGGTTCCAGAAGAAGGCGATCAGCGCCGCAGTGGCGGACATCGAGTCCGA
CATGAAGCACGGCCCGGCCAAGCGCGAGGAAGATGAAATGCAACGCGTGCTCTACCGCCGCGTGCTGGGGATCAGGGGGC
CGATGACCGAAGCTATGATGCCGCAGTTGATCGATGGCACGCCGCGGGGCGCCAGCGCGCAGGTGAAGCGGCGCGCGACA
GTGCATGCGTTGATCGATGCCAGGGCCAGCGCAGTATGA

Upstream 100 bases:

>100_bases
GAATGCGCGGCCGATACACCGCGTGCTGATCTTCCGCGCGCGCTTGCCCAGTTCGACAGGATTCGCGCGATCCTGGGCAC
GCAATCGGCCGAGTGACGAG

Downstream 100 bases:

>100_bases
AGCGGCTTTCCTTGCTCGAGCGTTCGCGCCTTCCGACCAATGATCTTGGCAATGCGCGCCGGCTGTTCGAGGCAGCAAAC
GGACGCCTGTTGTGGTTGGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MAIPHQFLDELRSRTTLSALIAQTLKIDKAGREFKACCPFHGEKTPSFTINDAKGFYHCFGCGAHGDAIRWMIDQRGLQF
IDAVSELASAAGMKVPAPTPQERERSAKIETVREALDIAQGIFVGQLREAGAVMQYLERRGMTPMDLEIFGIGYARGMDG
SLRGSGIGRQIGQAAGLLSQREDGSLREMFHDRVTVPIHDARGRLVGFGGRVWPGRRRDTPKFVNSPDSPIFDKGRTLFN
LHRAAPAARPGAENRLIVVEGYFDVVSMARAGFAACVAPMGTALTEEQLVRCWRLHHRPVLLFDGDSAGQKAAVRACRTA
IPGIGPGRELAIALLPDGKDPDELLYMDATGMGRRAIEAIVTEALPLHTFLFDAIVAEAGANPTPEQTAAVWAELAALAG
EIADEETRCQYLGTWRARFEREISALPQVHEAEPLHAVVAAEDGEYVFPESESDSQRRLIALVRAVLKKREERREINEEI
ADLMKMAELAGFQKKAISAAVADIESDMKHGPAKREEDEMQRVLYRRVLGIRGPMTEAMMPQLIDGTPRGASAQVKRRAT
VHALIDARASAV

Sequences:

>Translated_572_residues
MAIPHQFLDELRSRTTLSALIAQTLKIDKAGREFKACCPFHGEKTPSFTINDAKGFYHCFGCGAHGDAIRWMIDQRGLQF
IDAVSELASAAGMKVPAPTPQERERSAKIETVREALDIAQGIFVGQLREAGAVMQYLERRGMTPMDLEIFGIGYARGMDG
SLRGSGIGRQIGQAAGLLSQREDGSLREMFHDRVTVPIHDARGRLVGFGGRVWPGRRRDTPKFVNSPDSPIFDKGRTLFN
LHRAAPAARPGAENRLIVVEGYFDVVSMARAGFAACVAPMGTALTEEQLVRCWRLHHRPVLLFDGDSAGQKAAVRACRTA
IPGIGPGRELAIALLPDGKDPDELLYMDATGMGRRAIEAIVTEALPLHTFLFDAIVAEAGANPTPEQTAAVWAELAALAG
EIADEETRCQYLGTWRARFEREISALPQVHEAEPLHAVVAAEDGEYVFPESESDSQRRLIALVRAVLKKREERREINEEI
ADLMKMAELAGFQKKAISAAVADIESDMKHGPAKREEDEMQRVLYRRVLGIRGPMTEAMMPQLIDGTPRGASAQVKRRAT
VHALIDARASAV
>Mature_571_residues
AIPHQFLDELRSRTTLSALIAQTLKIDKAGREFKACCPFHGEKTPSFTINDAKGFYHCFGCGAHGDAIRWMIDQRGLQFI
DAVSELASAAGMKVPAPTPQERERSAKIETVREALDIAQGIFVGQLREAGAVMQYLERRGMTPMDLEIFGIGYARGMDGS
LRGSGIGRQIGQAAGLLSQREDGSLREMFHDRVTVPIHDARGRLVGFGGRVWPGRRRDTPKFVNSPDSPIFDKGRTLFNL
HRAAPAARPGAENRLIVVEGYFDVVSMARAGFAACVAPMGTALTEEQLVRCWRLHHRPVLLFDGDSAGQKAAVRACRTAI
PGIGPGRELAIALLPDGKDPDELLYMDATGMGRRAIEAIVTEALPLHTFLFDAIVAEAGANPTPEQTAAVWAELAALAGE
IADEETRCQYLGTWRARFEREISALPQVHEAEPLHAVVAAEDGEYVFPESESDSQRRLIALVRAVLKKREERREINEEIA
DLMKMAELAGFQKKAISAAVADIESDMKHGPAKREEDEMQRVLYRRVLGIRGPMTEAMMPQLIDGTPRGASAQVKRRATV
HALIDARASAV

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=416, Percent_Identity=39.6634615384615, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 62689; Mature: 62557

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPHQFLDELRSRTTLSALIAQTLKIDKAGREFKACCPFHGEKTPSFTINDAKGFYHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGDAIRWMIDQRGLQFIDAVSELASAAGMKVPAPTPQERERSAKIETVREALDIAQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GIFVGQLREAGAVMQYLERRGMTPMDLEIFGIGYARGMDGSLRGSGIGRQIGQAAGLLSQ
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHC
REDGSLREMFHDRVTVPIHDARGRLVGFGGRVWPGRRRDTPKFVNSPDSPIFDKGRTLFN
CCCCHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCHHCCCCCCCCHHCCCHHHH
LHRAAPAARPGAENRLIVVEGYFDVVSMARAGFAACVAPMGTALTEEQLVRCWRLHHRPV
HHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEE
LLFDGDSAGQKAAVRACRTAIPGIGPGRELAIALLPDGKDPDELLYMDATGMGRRAIEAI
EEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHH
VTEALPLHTFLFDAIVAEAGANPTPEQTAAVWAELAALAGEIADEETRCQYLGTWRARFE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
REISALPQVHEAEPLHAVVAAEDGEYVFPESESDSQRRLIALVRAVLKKREERREINEEI
HHHHHCCCCCCCCCCEEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLMKMAELAGFQKKAISAAVADIESDMKHGPAKREEDEMQRVLYRRVLGIRGPMTEAMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
PQLIDGTPRGASAQVKRRATVHALIDARASAV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIPHQFLDELRSRTTLSALIAQTLKIDKAGREFKACCPFHGEKTPSFTINDAKGFYHCF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGDAIRWMIDQRGLQFIDAVSELASAAGMKVPAPTPQERERSAKIETVREALDIAQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GIFVGQLREAGAVMQYLERRGMTPMDLEIFGIGYARGMDGSLRGSGIGRQIGQAAGLLSQ
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHC
REDGSLREMFHDRVTVPIHDARGRLVGFGGRVWPGRRRDTPKFVNSPDSPIFDKGRTLFN
CCCCHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCHHCCCCCCCCHHCCCHHHH
LHRAAPAARPGAENRLIVVEGYFDVVSMARAGFAACVAPMGTALTEEQLVRCWRLHHRPV
HHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEE
LLFDGDSAGQKAAVRACRTAIPGIGPGRELAIALLPDGKDPDELLYMDATGMGRRAIEAI
EEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHH
VTEALPLHTFLFDAIVAEAGANPTPEQTAAVWAELAALAGEIADEETRCQYLGTWRARFE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
REISALPQVHEAEPLHAVVAAEDGEYVFPESESDSQRRLIALVRAVLKKREERREINEEI
HHHHHCCCCCCCCCCEEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLMKMAELAGFQKKAISAAVADIESDMKHGPAKREEDEMQRVLYRRVLGIRGPMTEAMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
PQLIDGTPRGASAQVKRRATVHALIDARASAV
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]