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The map label for this gene is fixK [H]

Identifier: 87198515

GI number: 87198515

Start: 531932

End: 532702

Strand: Direct

Name: fixK [H]

Synonym: Saro_0490

Alternate gene names: 87198515

Gene position: 531932-532702 (Clockwise)

Preceding gene: 87198512

Following gene: 87198518

Centisome position: 14.94

GC content: 66.67

Gene sequence:

>771_bases
ATGGCAAGAGACGACGAAACCGGCCTCATGGTGGAGAATGCCGCAGCGCGGTGTGCACAGTGCCCGCTTGCGACGTGCGA
AGGGCTGCGCCAGCCACCCGATGAACTGCGCGCGTGGATCGCCGCGTTCCGCCAGGGCGAGGCGCGCTTTGTCAGGGGCG
AACAGGTCTTTGCGCAGGGCTTGCGCCTGCGGCATCTCTACACTGTCCTCTCAGGCGTGCTGATGCGTACCCGGCTGCTC
GAGGATGGGCGGCGCCAGATCATCAATTTCATGTTTCCGGGAGACTTCATCGGCCTGCAGGCGGCGCTTGACGGCGAAAT
GAGCCATGCGGTCGAGGCGGTGACGTCGGCCACGCTCTGCGTCTTTGCGCGGGACCGCTTCTTCGAACTGGCGGCACTTC
AGCCGCGTCTTTCGTTCGACCTGACATGGCTTGCCGCGCACGAGGAGGCGGCGCTCGAGGAACACATCGTCTCGCTCGGC
CAGCGCAACGCGCGCGAGCGGATGGCGGCCCTGGCGGTTTTTCTCGTCCAGCGAGGCATGGACACCGGGCTGGCCCCCGA
CGGTATCCTGCCGATCACCGTGACCCAGGGCCAGATCGCGGACATGTTGGGACTTTCGCTGGTCCATACCAACCGCACCT
TGCAGGCGCTGCGGCGGATGGGGCTGGTCGACTGGACGCTTTCCGCGATCCGCATCCCCGACATGGATGCCGCCCGGCGG
ATGGCACGGATCGACGGCGTCGGCGCCTCTGCGCCGCGTCCGTACATCTGA

Upstream 100 bases:

>100_bases
AATAACGGGTTCCCGTAGTGCACATCCCGGGAACAAACCCTGTCATTTGACATAGCGCATCCAGGGCGGGGCTGATTTGT
ACCGCCGACAGGGAGTGCGG

Downstream 100 bases:

>100_bases
AAATCGGCTCTGAAAAAAATTTCCAAAAAAGTTTCGCCCCCGGGAACCGCTCCACAAGACGCTCGTTAAGGGCATGTCAC
CGCCGAGACCCCCCCTCCCG

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MARDDETGLMVENAAARCAQCPLATCEGLRQPPDELRAWIAAFRQGEARFVRGEQVFAQGLRLRHLYTVLSGVLMRTRLL
EDGRRQIINFMFPGDFIGLQAALDGEMSHAVEAVTSATLCVFARDRFFELAALQPRLSFDLTWLAAHEEAALEEHIVSLG
QRNARERMAALAVFLVQRGMDTGLAPDGILPITVTQGQIADMLGLSLVHTNRTLQALRRMGLVDWTLSAIRIPDMDAARR
MARIDGVGASAPRPYI

Sequences:

>Translated_256_residues
MARDDETGLMVENAAARCAQCPLATCEGLRQPPDELRAWIAAFRQGEARFVRGEQVFAQGLRLRHLYTVLSGVLMRTRLL
EDGRRQIINFMFPGDFIGLQAALDGEMSHAVEAVTSATLCVFARDRFFELAALQPRLSFDLTWLAAHEEAALEEHIVSLG
QRNARERMAALAVFLVQRGMDTGLAPDGILPITVTQGQIADMLGLSLVHTNRTLQALRRMGLVDWTLSAIRIPDMDAARR
MARIDGVGASAPRPYI
>Mature_255_residues
ARDDETGLMVENAAARCAQCPLATCEGLRQPPDELRAWIAAFRQGEARFVRGEQVFAQGLRLRHLYTVLSGVLMRTRLLE
DGRRQIINFMFPGDFIGLQAALDGEMSHAVEAVTSATLCVFARDRFFELAALQPRLSFDLTWLAAHEEAALEEHIVSLGQ
RNARERMAALAVFLVQRGMDTGLAPDGILPITVTQGQIADMLGLSLVHTNRTLQALRRMGLVDWTLSAIRIPDMDAARRM
ARIDGVGASAPRPYI

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=191, Percent_Identity=27.2251308900524, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28313; Mature: 28182

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDDETGLMVENAAARCAQCPLATCEGLRQPPDELRAWIAAFRQGEARFVRGEQVFAQG
CCCCCCCCEEEHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCHHEECHHHHHHHH
LRLRHLYTVLSGVLMRTRLLEDGRRQIINFMFPGDFIGLQAALDGEMSHAVEAVTSATLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHH
VFARDRFFELAALQPRLSFDLTWLAAHEEAALEEHIVSLGQRNARERMAALAVFLVQRGM
HHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
DTGLAPDGILPITVTQGQIADMLGLSLVHTNRTLQALRRMGLVDWTLSAIRIPDMDAARR
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCHHHHHH
MARIDGVGASAPRPYI
HHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ARDDETGLMVENAAARCAQCPLATCEGLRQPPDELRAWIAAFRQGEARFVRGEQVFAQG
CCCCCCCEEEHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCHHEECHHHHHHHH
LRLRHLYTVLSGVLMRTRLLEDGRRQIINFMFPGDFIGLQAALDGEMSHAVEAVTSATLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHH
VFARDRFFELAALQPRLSFDLTWLAAHEEAALEEHIVSLGQRNARERMAALAVFLVQRGM
HHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
DTGLAPDGILPITVTQGQIADMLGLSLVHTNRTLQALRRMGLVDWTLSAIRIPDMDAARR
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCHHHHHH
MARIDGVGASAPRPYI
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]