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The map label for this gene is boxB [H]

Identifier: 86751751

GI number: 86751751

Start: 5262467

End: 5263912

Strand: Reverse

Name: boxB [H]

Synonym: RPB_4653

Alternate gene names: 86751751

Gene position: 5263912-5262467 (Counterclockwise)

Preceding gene: 86751752

Following gene: 86751749

Centisome position: 98.73

GC content: 62.72

Gene sequence:

>1446_bases
ATGAACATCATGAACGTCGACTACTCGACCAAGATTCCGAATAACGTCGATCTCGCCTCCGATCGTCAGGTGCTGAAGGC
CTTGGAAGGCTGGCACCCGGGATACATCGACTGGTGGAACGACATGGGCCCCGAGGGCTTCCAGCAGTCGCTGGTGTATC
TACGCACGGCCTATTCGGTCGATCCGCGCGGCTGGGCGAAATTCGACTACGTCAAGATGCCGGATTATCGCTGGGGCGTT
CTGCTGGCGCCACAGGAGGAGAACCGTAAGATCCCGTTCGGCGAACATTACGGCGAGCCGGCCTGGCAGGAAGTCCCCGG
CGAGTATCGTGCGATGCTGCGCCGCCTGATCGTGATCCAGGGCGACACCGAGCCGGCCTCGGTCGAGCAGCAGCGCCATC
TCGGCAAGACCGCGCCGTCGCTCTACGACATGCGCAACCTGTTCCAGGTCAATGTCGAAGAGGGGCGCCATCTGTGGGCG
ATGGTGTATCTCCTGCAGAAGTATTTCGGCCGCGACGGCCGCGAGGAGGCCGACGATCTGTTGCGCCGGCGCTCCGGCGA
CGCCGATTCGCCGCGGATGCTCGGCGCCTTCAACGAAGCGACGCCGGACTGGCTGTCGTTCTTCATGTTCACCTATTTCA
CCGATCGCGACGGCAAGATGCAGTTGCACTCGCTGGCGCAATCGGGCTTCGATCCGTTGTCGCGCACCTGCCGCTTCATG
CTGACCGAAGAAGCGCATCACATGTTCGTCGGCGAGACCGGCATCACCCGCATCATCCAGCGCACCTGCGACGCGATGCG
CGAGGCCGGCATCAGCGACCCGACGGACATTGCGAAAGTCCGCGCGCTCGGCGTCATCGATCTGCCGACGATCCAGAAGA
AGCTCAATCTGCACTACACGCTGTCGCTCGATCTGTTCGGCTCGGAAGTTTCGACCAACGCCGCCAACGCCTTCAACTTC
GGCATCAAGGGCCGCTACCACGAGACCCAGATCGACGATGATCACCAGCTCAAGAATTCGACGTATCCGGTGCTGAAATT
CGTCGACGGCGAGATCAGGCGCGTCGACGAGCCGGCGCTGACCGCGCTCAACATGCGGCTGCGCGACGACTACTCGCAGG
ATTGCGTCAAGGGCCTGCTGCGCTGGAACAAGGTGATCACCACCGCCGGCTACGACTATGCGCTGACGCTGCCGCACGTC
GCCTTTCACCGCGCCATCGGCGAGTTCAAGGACGTTCACGCGACGCCCGAAGGCATCCTGATCGACGGTGCGACCTGGGA
CAAGCGCAAGGACGAGTGGCTGCCGTCCACCGACGACGGCGACTTCATCGCCAGCCTGATGCAGCCGGTGAGCGACCCCG
GCGCCTACGCCCCGTGGATCTCGCCCCCCAAAGTCGGCATCGACAACAAGCCCGGCGATTTCGAATATGTGAAGATCGAG
ACCTGA

Upstream 100 bases:

>100_bases
TGAAACGCGGTGGTCGCACCCTTCGAGGCCCGCGCCGGGTGGCACGGGCACCTCAGGGTGACGGCGTCAGAACGGCAAGC
AGAGACCCAGGGAGCACGCC

Downstream 100 bases:

>100_bases
GCGCCTCGCGTCCCCGCCGGGGCGGCTGGTCTGCCGCCCCTCGGGCTTCATCGGAGGATTTGCCAGCGAAGTCTTGGAGG
CGGGCGTCCGCGCGTTGCGG

Product: benzoyl-CoA oxygenase component

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit B; Benzoyl-CoA dioxygenase oxygenase component [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNIMNVDYSTKIPNNVDLASDRQVLKALEGWHPGYIDWWNDMGPEGFQQSLVYLRTAYSVDPRGWAKFDYVKMPDYRWGV
LLAPQEENRKIPFGEHYGEPAWQEVPGEYRAMLRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWA
MVYLLQKYFGRDGREEADDLLRRRSGDADSPRMLGAFNEATPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFM
LTEEAHHMFVGETGITRIIQRTCDAMREAGISDPTDIAKVRALGVIDLPTIQKKLNLHYTLSLDLFGSEVSTNAANAFNF
GIKGRYHETQIDDDHQLKNSTYPVLKFVDGEIRRVDEPALTALNMRLRDDYSQDCVKGLLRWNKVITTAGYDYALTLPHV
AFHRAIGEFKDVHATPEGILIDGATWDKRKDEWLPSTDDGDFIASLMQPVSDPGAYAPWISPPKVGIDNKPGDFEYVKIE
T

Sequences:

>Translated_481_residues
MNIMNVDYSTKIPNNVDLASDRQVLKALEGWHPGYIDWWNDMGPEGFQQSLVYLRTAYSVDPRGWAKFDYVKMPDYRWGV
LLAPQEENRKIPFGEHYGEPAWQEVPGEYRAMLRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWA
MVYLLQKYFGRDGREEADDLLRRRSGDADSPRMLGAFNEATPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFM
LTEEAHHMFVGETGITRIIQRTCDAMREAGISDPTDIAKVRALGVIDLPTIQKKLNLHYTLSLDLFGSEVSTNAANAFNF
GIKGRYHETQIDDDHQLKNSTYPVLKFVDGEIRRVDEPALTALNMRLRDDYSQDCVKGLLRWNKVITTAGYDYALTLPHV
AFHRAIGEFKDVHATPEGILIDGATWDKRKDEWLPSTDDGDFIASLMQPVSDPGAYAPWISPPKVGIDNKPGDFEYVKIE
T
>Mature_481_residues
MNIMNVDYSTKIPNNVDLASDRQVLKALEGWHPGYIDWWNDMGPEGFQQSLVYLRTAYSVDPRGWAKFDYVKMPDYRWGV
LLAPQEENRKIPFGEHYGEPAWQEVPGEYRAMLRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWA
MVYLLQKYFGRDGREEADDLLRRRSGDADSPRMLGAFNEATPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFM
LTEEAHHMFVGETGITRIIQRTCDAMREAGISDPTDIAKVRALGVIDLPTIQKKLNLHYTLSLDLFGSEVSTNAANAFNF
GIKGRYHETQIDDDHQLKNSTYPVLKFVDGEIRRVDEPALTALNMRLRDDYSQDCVKGLLRWNKVITTAGYDYALTLPHV
AFHRAIGEFKDVHATPEGILIDGATWDKRKDEWLPSTDDGDFIASLMQPVSDPGAYAPWISPPKVGIDNKPGDFEYVKIE
T

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxB acts as the benzoyl-CoA oxygenase, after being reduced by the reductase component BoxA. BoxAB does not

COG id: COG3396

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the benzoyl-CoA oxygenase component B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017635
- InterPro:   IPR012347
- InterPro:   IPR009078
- InterPro:   IPR007814 [H]

Pfam domain/function: PF05138 PaaA_PaaC [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 55021; Mature: 55021

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIMNVDYSTKIPNNVDLASDRQVLKALEGWHPGYIDWWNDMGPEGFQQSLVYLRTAYSV
CCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DPRGWAKFDYVKMPDYRWGVLLAPQEENRKIPFGEHYGEPAWQEVPGEYRAMLRRLIVIQ
CCCCCCEECEEECCCCCCEEEECCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHEEEE
GDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWAMVYLLQKYFGRDGREEADDL
CCCCCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHCCCCCHHHHHH
LRRRSGDADSPRMLGAFNEATPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFM
HHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHCCCCHHHHHHHHH
LTEEAHHMFVGETGITRIIQRTCDAMREAGISDPTDIAKVRALGVIDLPTIQKKLNLHYT
HHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHCCEEEE
LSLDLFGSEVSTNAANAFNFGIKGRYHETQIDDDHQLKNSTYPVLKFVDGEIRRVDEPAL
EEEEEECCCCCCCCCCCEECCCCCCEECCCCCCCHHHCCCCCCEEEECCCHHHHCCCHHH
TALNMRLRDDYSQDCVKGLLRWNKVITTAGYDYALTLPHVAFHRAIGEFKDVHATPEGIL
HHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCEE
IDGATWDKRKDEWLPSTDDGDFIASLMQPVSDPGAYAPWISPPKVGIDNKPGDFEYVKIE
EECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
T
C
>Mature Secondary Structure
MNIMNVDYSTKIPNNVDLASDRQVLKALEGWHPGYIDWWNDMGPEGFQQSLVYLRTAYSV
CCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DPRGWAKFDYVKMPDYRWGVLLAPQEENRKIPFGEHYGEPAWQEVPGEYRAMLRRLIVIQ
CCCCCCEECEEECCCCCCEEEECCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHEEEE
GDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWAMVYLLQKYFGRDGREEADDL
CCCCCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHCCCCCHHHHHH
LRRRSGDADSPRMLGAFNEATPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFM
HHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHCCCCHHHHHHHHH
LTEEAHHMFVGETGITRIIQRTCDAMREAGISDPTDIAKVRALGVIDLPTIQKKLNLHYT
HHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHCCEEEE
LSLDLFGSEVSTNAANAFNFGIKGRYHETQIDDDHQLKNSTYPVLKFVDGEIRRVDEPAL
EEEEEECCCCCCCCCCCEECCCCCCEECCCCCCCHHHCCCCCCEEEECCCHHHHCCCHHH
TALNMRLRDDYSQDCVKGLLRWNKVITTAGYDYALTLPHVAFHRAIGEFKDVHATPEGIL
HHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCEE
IDGATWDKRKDEWLPSTDDGDFIASLMQPVSDPGAYAPWISPPKVGIDNKPGDFEYVKIE
EECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]