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The map label for this gene is pyrD

Identifier: 86751720

GI number: 86751720

Start: 5223342

End: 5224436

Strand: Reverse

Name: pyrD

Synonym: RPB_4622

Alternate gene names: 86751720

Gene position: 5224436-5223342 (Counterclockwise)

Preceding gene: 86751721

Following gene: 86751712

Centisome position: 97.99

GC content: 69.13

Gene sequence:

>1095_bases
GTGATCCGCGCCTTCGACGCGTTCTCGCTCCCGCTGCTGCGTCTGCTCGACGCCGAAGACGCCCACCGCCTCGCCATCCA
GGGGTTGCGGCTGCTGCCGCAGGTGAAGCCGCGCCCGGACGATTCCAAGCTCGCGGTGCGCGCCTTCGGGCTGAACTTCC
CCAATCCGGTCGGCATCGCCGCCGGTTTCGACAAGAATGCCGAAGCGCCGGATGCGCTGCTGCGGCTCGGCTTCGGCTTC
GTCGAGATCGGCACGGTGACGCCGAAGCCGCAGGCCGGCAATCCGCGGCCGCGGTTGTTCCGGCTGGAGCGCGACGAGGC
TATCATCAACCGGATGGGCTTCAACAATGACGGCGCCGAGGCCGTGCTACGCCGGCTTGCGGCGCGGGCGCAGCAGGGCG
GCATCGTCGGCGTCAATGTCGGCGCCAACAAGGACAGCACCGATCGCGTCGCCGACTACGTGTCGCTGATCGAGACCTTT
GCGCCGGTGGCGAGCTATTTCACCGTCAACGTGTCGTCGCCGAATACGCCGGGCCTGCGCAATCTGCAGCAGGCGGCGGC
GCTCGACGATCTGCTGGCGCGGGTGATCGAGGCCCGCGAACGGGTCCGCGCCAGCGCCGGCGACACTCCTGTGCTGCTGA
AGATTGCGCCCGACCTCACGCTCAGTGAACTCGACGACGTGGTGCACATCGCCCGCTCGCGCCGGGTCGACGGCATGATC
GTCGCCAACACCACGCTGTCGCGCTCCCCGATGCTGCGCGAACGGACGCGGCTGAACGAGCAGGGCGGCCTCAGCGGCCG
GCCGCTGTTCCGGCTGTCGACCCGGATGGTGGCGGAGACCTATGTCCGGGCCGAGGGCGCATTTCCGCTGATCGGCGTCG
GCGGCATCGATTCCGGCGGCGCGGCGCTGACCAAGATCCGCGCCGGCGCCAGCCTGGTGCAGCTGTATTCGGCGCTGATC
TACAAGGGCCTCGGCCTCGTCGACAGCATCAAGGCCGATCTCGCCTCGACGCTGCTGCGCACCGGGCGTGACTCGCTTTC
CGAAATCGTCGGTGCCGACGCGCCGACCATCACCGCGGAAGAGTGGCCGGTGTAA

Upstream 100 bases:

>100_bases
TTCCGCATCTCTACGGCGAACTCCATCTCGGCGCCGTCACTGGCATTCGCGACATCGCAACCTTTGCCGACGGTCGCCAC
GATCTCACGGAGGTCGCGCT

Downstream 100 bases:

>100_bases
GGGCTGTCAGATCGTCGTCATTCCGGGGCGCCGCGGAGCGGCGAACCCGGAGTCTGATACGAGCAGAGCATCTCGAGATT
CCGGGTTCGCGCCTGCGGCG

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MIRAFDAFSLPLLRLLDAEDAHRLAIQGLRLLPQVKPRPDDSKLAVRAFGLNFPNPVGIAAGFDKNAEAPDALLRLGFGF
VEIGTVTPKPQAGNPRPRLFRLERDEAIINRMGFNNDGAEAVLRRLAARAQQGGIVGVNVGANKDSTDRVADYVSLIETF
APVASYFTVNVSSPNTPGLRNLQQAAALDDLLARVIEARERVRASAGDTPVLLKIAPDLTLSELDDVVHIARSRRVDGMI
VANTTLSRSPMLRERTRLNEQGGLSGRPLFRLSTRMVAETYVRAEGAFPLIGVGGIDSGGAALTKIRAGASLVQLYSALI
YKGLGLVDSIKADLASTLLRTGRDSLSEIVGADAPTITAEEWPV

Sequences:

>Translated_364_residues
MIRAFDAFSLPLLRLLDAEDAHRLAIQGLRLLPQVKPRPDDSKLAVRAFGLNFPNPVGIAAGFDKNAEAPDALLRLGFGF
VEIGTVTPKPQAGNPRPRLFRLERDEAIINRMGFNNDGAEAVLRRLAARAQQGGIVGVNVGANKDSTDRVADYVSLIETF
APVASYFTVNVSSPNTPGLRNLQQAAALDDLLARVIEARERVRASAGDTPVLLKIAPDLTLSELDDVVHIARSRRVDGMI
VANTTLSRSPMLRERTRLNEQGGLSGRPLFRLSTRMVAETYVRAEGAFPLIGVGGIDSGGAALTKIRAGASLVQLYSALI
YKGLGLVDSIKADLASTLLRTGRDSLSEIVGADAPTITAEEWPV
>Mature_364_residues
MIRAFDAFSLPLLRLLDAEDAHRLAIQGLRLLPQVKPRPDDSKLAVRAFGLNFPNPVGIAAGFDKNAEAPDALLRLGFGF
VEIGTVTPKPQAGNPRPRLFRLERDEAIINRMGFNNDGAEAVLRRLAARAQQGGIVGVNVGANKDSTDRVADYVSLIETF
APVASYFTVNVSSPNTPGLRNLQQAAALDDLLARVIEARERVRASAGDTPVLLKIAPDLTLSELDDVVHIARSRRVDGMI
VANTTLSRSPMLRERTRLNEQGGLSGRPLFRLSTRMVAETYVRAEGAFPLIGVGGIDSGGAALTKIRAGASLVQLYSALI
YKGLGLVDSIKADLASTLLRTGRDSLSEIVGADAPTITAEEWPV

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=361, Percent_Identity=49.0304709141274, Blast_Score=308, Evalue=4e-84,
Organism=Escherichia coli, GI1787177, Length=322, Percent_Identity=44.7204968944099, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17509475, Length=356, Percent_Identity=44.6629213483146, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI281361352, Length=358, Percent_Identity=48.3240223463687, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI17137316, Length=358, Percent_Identity=48.3240223463687, Blast_Score=327, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_RHOP2 (Q2IR55)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_488216.1
- ProteinModelPortal:   Q2IR55
- SMR:   Q2IR55
- STRING:   Q2IR55
- GeneID:   3912439
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_4622
- eggNOG:   COG0167
- HOGENOM:   HBG351027
- OMA:   AALNRMG
- ProtClustDB:   PRK05286
- BioCyc:   RPAL316058:RPB_4622-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 39049; Mature: 39049

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRAFDAFSLPLLRLLDAEDAHRLAIQGLRLLPQVKPRPDDSKLAVRAFGLNFPNPVGIA
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEE
AGFDKNAEAPDALLRLGFGFVEIGTVTPKPQAGNPRPRLFRLERDEAIINRMGFNNDGAE
CCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCHH
AVLRRLAARAQQGGIVGVNVGANKDSTDRVADYVSLIETFAPVASYFTVNVSSPNTPGLR
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHH
NLQQAAALDDLLARVIEARERVRASAGDTPVLLKIAPDLTLSELDDVVHIARSRRVDGMI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEE
VANTTLSRSPMLRERTRLNEQGGLSGRPLFRLSTRMVAETYVRAEGAFPLIGVGGIDSGG
EECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AALTKIRAGASLVQLYSALIYKGLGLVDSIKADLASTLLRTGRDSLSEIVGADAPTITAE
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCC
EWPV
CCCC
>Mature Secondary Structure
MIRAFDAFSLPLLRLLDAEDAHRLAIQGLRLLPQVKPRPDDSKLAVRAFGLNFPNPVGIA
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEE
AGFDKNAEAPDALLRLGFGFVEIGTVTPKPQAGNPRPRLFRLERDEAIINRMGFNNDGAE
CCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCHH
AVLRRLAARAQQGGIVGVNVGANKDSTDRVADYVSLIETFAPVASYFTVNVSSPNTPGLR
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHH
NLQQAAALDDLLARVIEARERVRASAGDTPVLLKIAPDLTLSELDDVVHIARSRRVDGMI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEE
VANTTLSRSPMLRERTRLNEQGGLSGRPLFRLSTRMVAETYVRAEGAFPLIGVGGIDSGG
EECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AALTKIRAGASLVQLYSALIYKGLGLVDSIKADLASTLLRTGRDSLSEIVGADAPTITAE
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCC
EWPV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA