Click here to switch to the map view.

The map label for this gene is fadA [H]

Identifier: 86751703

GI number: 86751703

Start: 5200023

End: 5201231

Strand: Reverse

Name: fadA [H]

Synonym: RPB_4605

Alternate gene names: 86751703

Gene position: 5201231-5200023 (Counterclockwise)

Preceding gene: 86751704

Following gene: 86751702

Centisome position: 97.55

GC content: 66.25

Gene sequence:

>1209_bases
ATGCCCGAAGCCTATATCTACGATCACGTTCGCACGCCTCGCGGCCGCGGCAAGGCCGACGGCGCCTTGCACGAGGTGAC
CGCGCTGGCGCTCGCCACCGTACCGCTGAAAGCGCTGAAGGAACGCAACAACCTGAAGCAGGACGTGGTCGACGACGTCA
TCCTCGGCGTGGTCGATCCGGTCGGTGAGGCCGGCTCGGACATCGCGCGGTTCGCGGCGATGAACGCGGGCCTCGGCGAA
GCCGTGCCGGGCATCCAGATCAGCCGCTTCTGTGCCTCGGGCCTCGACGCGGTGAACTTCGCTGCGGCGCAGATCATGAG
CGGCCAGCACGAGCTGGTGATCGGCGGCGGCGCGGAATCGATGAGCCGCATCGGCATCGGCGCCTCCGGCGGCGCCTGGC
CGATGGACCCGTCGATGGCTGTGCCGTCCTACTTCATGCCGCAGGGCATTTCGGCCGATTTGATCGCGACCAAATACGGT
TTCTCGCGGGACGACGTCGACGCTTATGCGGTGCAGAGCCAGCAGCGCTCGGCGAAGTCGTGGGAAGAAGGCCGCTTCGC
CAAATCGGTCGTGCCGGTCAAGGACATCAACGGCCTGACCATTCTGGCCAAGGACGAGCACATGCGGCCATCGACGACGA
TGCAGTCGCTCGGGCAGTTGCAGCCGTCGTTCGCGCCGATGGCGGTGATGGGCGGTTTCGACGCGGTGGCGATCCAGTCG
CATCCGGAGATCGAGAAGGTCAACTACGTCCATCATGCCGGCAACTCTTCCGGCATCGTCGATGGCGCCGGCGCGGTGCT
GCTCGGCAGCAAGGAAGCCGGTGCCAAGCACGGCCTCAAGCCGCGCGCAAAAATTCGCGCCTTCGCCAATATCGGCTCCG
AGCCGGCGATGATGCTGACCGGCCCGGTCGACGTCACCAAGAAGCTGTTCGAGCGCTCCGGCATGAAGAAGAGCGACATC
GACCTGTTCGAGCTCAACGAGGCTTTCGCCTCGGTGGTGCTGCGCTTCATGCAGGCGTTCGAGATCGACAACGACCAGAT
CAACGTGACCGGCGGCGCGATCGCGCTCGGCCATCCGCTCGGCGCGACCGGCGCGATGATCCTCGGCACCGTGCTCGACG
AGCTCGAGCGCACCGGCAAGGCGACCGCGCTGGTGACGCTGTGCATCGGCGGCGGCATGGGCACCGCGACGATCATCGAA
CGCGTCTGA

Upstream 100 bases:

>100_bases
TGGATTGCTTCGTCGCTATCGCTCCTCGCAATGACGAAATGACACGCGATTGTTCAATTCGTCCGGCATCCTGAGCACGG
ACCGATACGGGAGAGAACCG

Downstream 100 bases:

>100_bases
CGCCGTAGGGTGGGCAAAGCGAAGCGTGCCCACCATCGCATTCATTGCGTGAACGTGGGCACGGCGCGAGGCGCGCCTTT
GCCCACCCTACAAACACCCA

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPEAYIYDHVRTPRGRGKADGALHEVTALALATVPLKALKERNNLKQDVVDDVILGVVDPVGEAGSDIARFAAMNAGLGE
AVPGIQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAESMSRIGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYG
FSRDDVDAYAVQSQQRSAKSWEEGRFAKSVVPVKDINGLTILAKDEHMRPSTTMQSLGQLQPSFAPMAVMGGFDAVAIQS
HPEIEKVNYVHHAGNSSGIVDGAGAVLLGSKEAGAKHGLKPRAKIRAFANIGSEPAMMLTGPVDVTKKLFERSGMKKSDI
DLFELNEAFASVVLRFMQAFEIDNDQINVTGGAIALGHPLGATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIE
RV

Sequences:

>Translated_402_residues
MPEAYIYDHVRTPRGRGKADGALHEVTALALATVPLKALKERNNLKQDVVDDVILGVVDPVGEAGSDIARFAAMNAGLGE
AVPGIQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAESMSRIGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYG
FSRDDVDAYAVQSQQRSAKSWEEGRFAKSVVPVKDINGLTILAKDEHMRPSTTMQSLGQLQPSFAPMAVMGGFDAVAIQS
HPEIEKVNYVHHAGNSSGIVDGAGAVLLGSKEAGAKHGLKPRAKIRAFANIGSEPAMMLTGPVDVTKKLFERSGMKKSDI
DLFELNEAFASVVLRFMQAFEIDNDQINVTGGAIALGHPLGATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIE
RV
>Mature_401_residues
PEAYIYDHVRTPRGRGKADGALHEVTALALATVPLKALKERNNLKQDVVDDVILGVVDPVGEAGSDIARFAAMNAGLGEA
VPGIQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAESMSRIGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYGF
SRDDVDAYAVQSQQRSAKSWEEGRFAKSVVPVKDINGLTILAKDEHMRPSTTMQSLGQLQPSFAPMAVMGGFDAVAIQSH
PEIEKVNYVHHAGNSSGIVDGAGAVLLGSKEAGAKHGLKPRAKIRAFANIGSEPAMMLTGPVDVTKKLFERSGMKKSDID
LFELNEAFASVVLRFMQAFEIDNDQINVTGGAIALGHPLGATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIER
V

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=34.4221105527638, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI167614485, Length=404, Percent_Identity=33.6633663366337, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=33.8461538461538, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4557237, Length=415, Percent_Identity=29.1566265060241, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=29.6629213483146, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI194353979, Length=125, Percent_Identity=39.2, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=40.8450704225352, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI48994986, Length=410, Percent_Identity=35.8536585365854, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI87082165, Length=417, Percent_Identity=37.410071942446, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1788554, Length=419, Percent_Identity=36.5155131264916, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788683, Length=427, Percent_Identity=27.6346604215457, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=32.1695760598504, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=412, Percent_Identity=31.7961165048544, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17535921, Length=413, Percent_Identity=29.7820823244552, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=448, Percent_Identity=27.0089285714286, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17535917, Length=146, Percent_Identity=32.1917808219178, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322031, Length=359, Percent_Identity=32.8690807799443, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6325229, Length=419, Percent_Identity=30.3102625298329, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655093, Length=417, Percent_Identity=38.3693045563549, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI17648125, Length=413, Percent_Identity=33.8983050847458, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24640423, Length=416, Percent_Identity=30.2884615384615, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI17137578, Length=447, Percent_Identity=27.7404921700224, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42160; Mature: 42029

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAYIYDHVRTPRGRGKADGALHEVTALALATVPLKALKERNNLKQDVVDDVILGVVDP
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
VGEAGSDIARFAAMNAGLGEAVPGIQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAES
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCHHH
MSRIGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYGFSRDDVDAYAVQSQQRSAKS
HHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHECCCCCCCCCHHHHHHHHHHHHHH
WEEGRFAKSVVPVKDINGLTILAKDEHMRPSTTMQSLGQLQPSFAPMAVMGGFDAVAIQS
HHHCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCEEEECC
HPEIEKVNYVHHAGNSSGIVDGAGAVLLGSKEAGAKHGLKPRAKIRAFANIGSEPAMMLT
CCCHHHHHHEEECCCCCCCCCCCCEEEEECCCCCHHCCCCCHHHHHHHHHCCCCCCEEEE
GPVDVTKKLFERSGMKKSDIDLFELNEAFASVVLRFMQAFEIDNDQINVTGGAIALGHPL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCC
GATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIERV
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
PEAYIYDHVRTPRGRGKADGALHEVTALALATVPLKALKERNNLKQDVVDDVILGVVDP
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
VGEAGSDIARFAAMNAGLGEAVPGIQISRFCASGLDAVNFAAAQIMSGQHELVIGGGAES
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCHHH
MSRIGIGASGGAWPMDPSMAVPSYFMPQGISADLIATKYGFSRDDVDAYAVQSQQRSAKS
HHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHECCCCCCCCCHHHHHHHHHHHHHH
WEEGRFAKSVVPVKDINGLTILAKDEHMRPSTTMQSLGQLQPSFAPMAVMGGFDAVAIQS
HHHCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCEEEECC
HPEIEKVNYVHHAGNSSGIVDGAGAVLLGSKEAGAKHGLKPRAKIRAFANIGSEPAMMLT
CCCHHHHHHEEECCCCCCCCCCCCEEEEECCCCCHHCCCCCHHHHHHHHHCCCCCCEEEE
GPVDVTKKLFERSGMKKSDIDLFELNEAFASVVLRFMQAFEIDNDQINVTGGAIALGHPL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCC
GATGAMILGTVLDELERTGKATALVTLCIGGGMGTATIIERV
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]