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The map label for this gene is thrC [H]

Identifier: 86751676

GI number: 86751676

Start: 5174593

End: 5176011

Strand: Reverse

Name: thrC [H]

Synonym: RPB_4578

Alternate gene names: 86751676

Gene position: 5176011-5174593 (Counterclockwise)

Preceding gene: 86751677

Following gene: 86751675

Centisome position: 97.08

GC content: 66.6

Gene sequence:

>1419_bases
TTGACGACCTATGTTTCCACCCGGGGCGAAGCCCCGCAGCTCGGCTTTTGCGATGTGATGCTGACCGGGCTCGCCCGTGA
CGGCGGGCTCTACATCCCTGACGTCTGGCCGCAGCTTTCGCCCGACGCCATCGCCGCTTTGTTCGGCCGGCCGTATTGGG
AGGTCGCCGTCGAGGTGATCCGGCCGTTCGTCGCCGGCGAGATTTCCGATGCCGAGCTCGGCCGGATGGCCAATGAAGCC
TACGCCACCTTCCGGCATCCCGCCGTGGTGCCGCTGCGCCAGAGCGGGTCGAGCCAGTTCGTGCTCGAGCTGTTTCACGG
TCCGACGCTGGCGTTCAAGGACGTCGCGATGCAGTTGCTGTCGCGGCTGATGGATCACGTGCTGGAGAAGCGCCGGCAGC
GCACCACCATCGTGGTCGCGACCTCCGGCGACACCGGCGGCGCCGCGGTCGATGCCTTCGCCAATTTGAACAATGTCGAT
CTGGTGGTGCTGTTTCCGCATGGCCGGATCTCCGACGTGCAGCGGATGATGATGACGACGTCGGGCGCGCCGAATGTGCA
TGCGCTGGCGATCGAGGGCCATTTCGACGATTGCCAGGCGATCGTGAAGGGGCTGTTCAACCACCACGCCTTCCGCGACA
AGGTGGCGCTGTCGGGCGTCAATTCGATCAACTGGGCGCGGATCGTCGCCCAGGTGGTGTACTACTTCACCAGCGCGGTG
GCGCTCGGCGCGCCCAACCGCGTCGTCGATTTCACCGTGCCGACCGGCAATTTCGGCGACATCTTCGCCGGCTATGTCGC
CAAGAAAATGGGCCTGCCGGTGCGGCGGCTGAAGATCGCCGCCAACACCAACGACATTCTGGCGCGGACGCTGAAGACCG
GGATCTACGAGGTCCGCGAGGTCCATTCCACGACCTCGCCGTCGATGGACATTCAGGTGTCGTCGAATTTCGAACGGTTG
CTGTTCGAGGCGACCGGCCGCGACGCCGCGCAGGTGCGGGCGCTGATGGGGTCGCTGCACCAGTCCGGCCGGTTCGTGCT
GCCGGATGCCGTGCTGGCGGCGATCCGCGAGGAATTCGACGCCGGCCGTGCCGACGAGACCGAGACCGAGGCCGCGATCC
GTACTGCTTGGCGCGAGGCCGGCGATCTGGTCGATCCGCACACCGCGGTGGCGCTGGCGGTCGCCGATCGCGACACCACG
GACCCGACCGTGCCCAATATCGTTCTGTCCACGGCGCACGCCGCCAAATTCCCCGACGCGGTCGAGGCCGCCTGCGGAAT
GCGGCCGGAACTGCCGGCCTGGCTCGGCGGGCTGATGAGCGGCACCGAAACGATGAAGATCATGCCGCGCGACCAGGCGG
CGATCGAACGCCACATCCTGTCTGTCAGTCGGGCGGCGAAACAAGGAGCAGTCGGATGA

Upstream 100 bases:

>100_bases
GCGGGAAAACCCTCTATGGTGCCCGGGATTTCACCCGTCGTGAGACGAATAGTATTCGCTATCAAAGGCTTGGCCGCGGC
CTAGGCCTTTGGAGGATGCC

Downstream 100 bases:

>100_bases
GCGTCGAAGTCAGCAAGCTTCCCTCGGGCCTGACCGTCGTCACCGACACCATGCCGCATCTCGAGACCGCGGCGCTGGGC
GTCTGGACCGGCGTCGGCGG

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTTYVSTRGEAPQLGFCDVMLTGLARDGGLYIPDVWPQLSPDAIAALFGRPYWEVAVEVIRPFVAGEISDAELGRMANEA
YATFRHPAVVPLRQSGSSQFVLELFHGPTLAFKDVAMQLLSRLMDHVLEKRRQRTTIVVATSGDTGGAAVDAFANLNNVD
LVVLFPHGRISDVQRMMMTTSGAPNVHALAIEGHFDDCQAIVKGLFNHHAFRDKVALSGVNSINWARIVAQVVYYFTSAV
ALGAPNRVVDFTVPTGNFGDIFAGYVAKKMGLPVRRLKIAANTNDILARTLKTGIYEVREVHSTTSPSMDIQVSSNFERL
LFEATGRDAAQVRALMGSLHQSGRFVLPDAVLAAIREEFDAGRADETETEAAIRTAWREAGDLVDPHTAVALAVADRDTT
DPTVPNIVLSTAHAAKFPDAVEAACGMRPELPAWLGGLMSGTETMKIMPRDQAAIERHILSVSRAAKQGAVG

Sequences:

>Translated_472_residues
MTTYVSTRGEAPQLGFCDVMLTGLARDGGLYIPDVWPQLSPDAIAALFGRPYWEVAVEVIRPFVAGEISDAELGRMANEA
YATFRHPAVVPLRQSGSSQFVLELFHGPTLAFKDVAMQLLSRLMDHVLEKRRQRTTIVVATSGDTGGAAVDAFANLNNVD
LVVLFPHGRISDVQRMMMTTSGAPNVHALAIEGHFDDCQAIVKGLFNHHAFRDKVALSGVNSINWARIVAQVVYYFTSAV
ALGAPNRVVDFTVPTGNFGDIFAGYVAKKMGLPVRRLKIAANTNDILARTLKTGIYEVREVHSTTSPSMDIQVSSNFERL
LFEATGRDAAQVRALMGSLHQSGRFVLPDAVLAAIREEFDAGRADETETEAAIRTAWREAGDLVDPHTAVALAVADRDTT
DPTVPNIVLSTAHAAKFPDAVEAACGMRPELPAWLGGLMSGTETMKIMPRDQAAIERHILSVSRAAKQGAVG
>Mature_471_residues
TTYVSTRGEAPQLGFCDVMLTGLARDGGLYIPDVWPQLSPDAIAALFGRPYWEVAVEVIRPFVAGEISDAELGRMANEAY
ATFRHPAVVPLRQSGSSQFVLELFHGPTLAFKDVAMQLLSRLMDHVLEKRRQRTTIVVATSGDTGGAAVDAFANLNNVDL
VVLFPHGRISDVQRMMMTTSGAPNVHALAIEGHFDDCQAIVKGLFNHHAFRDKVALSGVNSINWARIVAQVVYYFTSAVA
LGAPNRVVDFTVPTGNFGDIFAGYVAKKMGLPVRRLKIAANTNDILARTLKTGIYEVREVHSTTSPSMDIQVSSNFERLL
FEATGRDAAQVRALMGSLHQSGRFVLPDAVLAAIREEFDAGRADETETEAAIRTAWREAGDLVDPHTAVALAVADRDTTD
PTVPNIVLSTAHAAKFPDAVEAACGMRPELPAWLGGLMSGTETMKIMPRDQAAIERHILSVSRAAKQGAVG

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI149193323, Length=457, Percent_Identity=37.199124726477, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI153792148, Length=458, Percent_Identity=36.8995633187773, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1786185, Length=450, Percent_Identity=32.6666666666667, Blast_Score=215, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6319901, Length=459, Percent_Identity=40.7407407407407, Blast_Score=322, Evalue=6e-89,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 51022; Mature: 50891

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYVSTRGEAPQLGFCDVMLTGLARDGGLYIPDVWPQLSPDAIAALFGRPYWEVAVEVI
CCCEECCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHCCHHHHHHHHHH
RPFVAGEISDAELGRMANEAYATFRHPAVVPLRQSGSSQFVLELFHGPTLAFKDVAMQLL
HHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEHHHHCCCCHHHHHHHHHHH
SRLMDHVLEKRRQRTTIVVATSGDTGGAAVDAFANLNNVDLVVLFPHGRISDVQRMMMTT
HHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHC
SGAPNVHALAIEGHFDDCQAIVKGLFNHHAFRDKVALSGVNSINWARIVAQVVYYFTSAV
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ALGAPNRVVDFTVPTGNFGDIFAGYVAKKMGLPVRRLKIAANTNDILARTLKTGIYEVRE
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
VHSTTSPSMDIQVSSNFERLLFEATGRDAAQVRALMGSLHQSGRFVLPDAVLAAIREEFD
HHCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHC
AGRADETETEAAIRTAWREAGDLVDPHTAVALAVADRDTTDPTVPNIVLSTAHAAKFPDA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHEECCHHHCCCHH
VEAACGMRPELPAWLGGLMSGTETMKIMPRDQAAIERHILSVSRAAKQGAVG
HHHHCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTYVSTRGEAPQLGFCDVMLTGLARDGGLYIPDVWPQLSPDAIAALFGRPYWEVAVEVI
CCEECCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHCCHHHHHHHHHH
RPFVAGEISDAELGRMANEAYATFRHPAVVPLRQSGSSQFVLELFHGPTLAFKDVAMQLL
HHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEHHHHCCCCHHHHHHHHHHH
SRLMDHVLEKRRQRTTIVVATSGDTGGAAVDAFANLNNVDLVVLFPHGRISDVQRMMMTT
HHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHC
SGAPNVHALAIEGHFDDCQAIVKGLFNHHAFRDKVALSGVNSINWARIVAQVVYYFTSAV
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ALGAPNRVVDFTVPTGNFGDIFAGYVAKKMGLPVRRLKIAANTNDILARTLKTGIYEVRE
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
VHSTTSPSMDIQVSSNFERLLFEATGRDAAQVRALMGSLHQSGRFVLPDAVLAAIREEFD
HHCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHC
AGRADETETEAAIRTAWREAGDLVDPHTAVALAVADRDTTDPTVPNIVLSTAHAAKFPDA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHEECCHHHCCCHH
VEAACGMRPELPAWLGGLMSGTETMKIMPRDQAAIERHILSVSRAAKQGAVG
HHHHCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1333566; 10984043 [H]