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The map label for this gene is gltD [H]

Identifier: 86751628

GI number: 86751628

Start: 5118767

End: 5120221

Strand: Reverse

Name: gltD [H]

Synonym: RPB_4530

Alternate gene names: 86751628

Gene position: 5120221-5118767 (Counterclockwise)

Preceding gene: 86751629

Following gene: 86751627

Centisome position: 96.03

GC content: 67.01

Gene sequence:

>1455_bases
ATGGGCAAGATCACGGGATTTCTGGAAATCGAACGGCACGATCGGGTCTATGCGCCGGTCGCCGAGCGGGTGAAGAACTA
CAACGAATTCGTCGTCCCGCTCAGCGACAAGGACCTCGGCGACCAGGCCGCGCGCTGCATGAATTGCGGCATTCCGTATT
GCCACGGCACCGGCTCGGCGCAGCCGGGCGCGCCGGGCTGCCCGGTCAACAACCAGATCCCCGACTGGAACGATCTGGTG
TACCAGGGCAATTGGCAGGAGGCCTCGCGCAACCTGCACTCCACCAACAACTTCCCGGAATTCACCGGCCGCATCTGCCC
GGCGCCGTGCGAAGCCTCGTGCACGCTGAACATCGACGACAACCCGGTCACCATCAAGACCATCGAATGCGCCATCGTCG
ACCGCGCCTGGGAGAACGGCTGGCTCGTGCCCGAGCTCGCCTCGGTCAAGACCGGCCAGAGCGTCGCGGTGATCGGCGCC
GGCCCGGCGGGCCTCGCCTGCGCCCAGCAGTTGGCGCGCGCCGGCCATGACGTCCACGTCTATGAGAAGCTCGCCAAGGC
CGGCGGCCTGCTGCGCTACGGCATTCCCGACTTCAAGATGGAGAAGCATCTGATCGACCGCCGCGTCGCGCAGATGGAAG
CCGAAGGCGTCACCTTCCACTACAATGCACCGGTCGGCGGCAATGCGGCCGGCGCGGTCGATCCGGCCGAGCTGCTGAAG
CAGTACGACGCCGTGGCCTTGACCGGCGGCGCGGAATATCCGCGCGACCTGCCGATCCCGGGCCGCGAGCTCTCCGGCAT
CCATTTCGCGATGGACTTCCTGACCCAGCAGAACCGCCGCGTCTCCGGCGAAGCGGTGAACGGCGCCGACATTCTGGCGA
CCGGCAAGCACGTCGTGGTGATCGGCGGCGGCGACACCGGCTCGGACTGCATCGGCACCTCGATCCGTCAGGGCGCCACC
TCGGTGACCCAGATCGAGATCATGGCGGCGCCGCCGGAGAAGGAAGACAAGGGCCTGACCTGGCCGAACTGGCCGATGAA
GATGCGCACCTCGTCGAGCCAGGCCGAAGGCGCGGTCCGCGAATTCGCGGTGCTGACGCAGAGCTTCACGGGCGAGAACG
GCGCGGTGAAGCAGCTCGATTGCGTCCGCGTCGACGCCAAATTCCAGCCGATCCCCGGCACCGAATTCACGCTGCAGGCC
GATCTGGTGCTGCTGGCGATGGGCTTCGTCTCGCCGGTCAAGCAAGGCCTGCTCGAAAAACTCGGCGTCGCCCTCGACCC
GCGCGGCAATGTCAGCGCCGACCTGTCGCACTTCACCACCTCGGTCGACAAAGTCTTCGCCGCCGGCGACATGCGCCGCG
GCCAGTCGCTGGTCGTCTGGGCGATCCGCGAAGGCCGCCTGTGCGCCCGGGCGATCGATCAGTTCCTGATGGGAAGCACG
ACGCTGCCGAAGTAA

Upstream 100 bases:

>100_bases
AGCAGCAGAACAGTCATCCTTCGAGGCTCGCCCAAGCGGGCGAGCGCCTCAGGATGACGGCTCAGGATGAATTCGAAGGT
TCAGGGGCGTTGGTAGTCTG

Downstream 100 bases:

>100_bases
GGGGGGACGGCCCCTTACTTCTCCCTCCCCCTTGTGGGGAGGGTGGCCCGGCGTAGCGCAGCGAAGCCGGGACGGGTGGG
GGGCCGCGGGCAGGGCGCCA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MGKITGFLEIERHDRVYAPVAERVKNYNEFVVPLSDKDLGDQAARCMNCGIPYCHGTGSAQPGAPGCPVNNQIPDWNDLV
YQGNWQEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDDNPVTIKTIECAIVDRAWENGWLVPELASVKTGQSVAVIGA
GPAGLACAQQLARAGHDVHVYEKLAKAGGLLRYGIPDFKMEKHLIDRRVAQMEAEGVTFHYNAPVGGNAAGAVDPAELLK
QYDAVALTGGAEYPRDLPIPGRELSGIHFAMDFLTQQNRRVSGEAVNGADILATGKHVVVIGGGDTGSDCIGTSIRQGAT
SVTQIEIMAAPPEKEDKGLTWPNWPMKMRTSSSQAEGAVREFAVLTQSFTGENGAVKQLDCVRVDAKFQPIPGTEFTLQA
DLVLLAMGFVSPVKQGLLEKLGVALDPRGNVSADLSHFTTSVDKVFAAGDMRRGQSLVVWAIREGRLCARAIDQFLMGST
TLPK

Sequences:

>Translated_484_residues
MGKITGFLEIERHDRVYAPVAERVKNYNEFVVPLSDKDLGDQAARCMNCGIPYCHGTGSAQPGAPGCPVNNQIPDWNDLV
YQGNWQEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDDNPVTIKTIECAIVDRAWENGWLVPELASVKTGQSVAVIGA
GPAGLACAQQLARAGHDVHVYEKLAKAGGLLRYGIPDFKMEKHLIDRRVAQMEAEGVTFHYNAPVGGNAAGAVDPAELLK
QYDAVALTGGAEYPRDLPIPGRELSGIHFAMDFLTQQNRRVSGEAVNGADILATGKHVVVIGGGDTGSDCIGTSIRQGAT
SVTQIEIMAAPPEKEDKGLTWPNWPMKMRTSSSQAEGAVREFAVLTQSFTGENGAVKQLDCVRVDAKFQPIPGTEFTLQA
DLVLLAMGFVSPVKQGLLEKLGVALDPRGNVSADLSHFTTSVDKVFAAGDMRRGQSLVVWAIREGRLCARAIDQFLMGST
TLPK
>Mature_483_residues
GKITGFLEIERHDRVYAPVAERVKNYNEFVVPLSDKDLGDQAARCMNCGIPYCHGTGSAQPGAPGCPVNNQIPDWNDLVY
QGNWQEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDDNPVTIKTIECAIVDRAWENGWLVPELASVKTGQSVAVIGAG
PAGLACAQQLARAGHDVHVYEKLAKAGGLLRYGIPDFKMEKHLIDRRVAQMEAEGVTFHYNAPVGGNAAGAVDPAELLKQ
YDAVALTGGAEYPRDLPIPGRELSGIHFAMDFLTQQNRRVSGEAVNGADILATGKHVVVIGGGDTGSDCIGTSIRQGATS
VTQIEIMAAPPEKEDKGLTWPNWPMKMRTSSSQAEGAVREFAVLTQSFTGENGAVKQLDCVRVDAKFQPIPGTEFTLQAD
LVLLAMGFVSPVKQGLLEKLGVALDPRGNVSADLSHFTTSVDKVFAAGDMRRGQSLVVWAIREGRLCARAIDQFLMGSTT
LPK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=464, Percent_Identity=23.9224137931034, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1789606, Length=490, Percent_Identity=37.9591836734694, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1788811, Length=477, Percent_Identity=36.6876310272537, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082180, Length=474, Percent_Identity=33.3333333333333, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788468, Length=460, Percent_Identity=28.0434782608696, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1789243, Length=427, Percent_Identity=27.1662763466042, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=503, Percent_Identity=42.3459244532803, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71984108, Length=309, Percent_Identity=26.537216828479, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=160, Percent_Identity=31.25, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=499, Percent_Identity=42.685370741483, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28574881, Length=495, Percent_Identity=48.8888888888889, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665539, Length=495, Percent_Identity=48.8888888888889, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665547, Length=495, Percent_Identity=48.8888888888889, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665543, Length=495, Percent_Identity=48.8888888888889, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24640763, Length=308, Percent_Identity=25.6493506493506, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18858217, Length=308, Percent_Identity=25.6493506493506, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51986; Mature: 51854

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKITGFLEIERHDRVYAPVAERVKNYNEFVVPLSDKDLGDQAARCMNCGIPYCHGTGSA
CCCEEEEEEEECCCCEEHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCC
QPGAPGCPVNNQIPDWNDLVYQGNWQEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDD
CCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEECC
NPVTIKTIECAIVDRAWENGWLVPELASVKTGQSVAVIGAGPAGLACAQQLARAGHDVHV
CCEEEEEEEEEEEECCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHH
YEKLAKAGGLLRYGIPDFKMEKHLIDRRVAQMEAEGVTFHYNAPVGGNAAGAVDPAELLK
HHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHH
QYDAVALTGGAEYPRDLPIPGRELSGIHFAMDFLTQQNRRVSGEAVNGADILATGKHVVV
HCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEE
IGGGDTGSDCIGTSIRQGATSVTQIEIMAAPPEKEDKGLTWPNWPMKMRTSSSQAEGAVR
EECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
EFAVLTQSFTGENGAVKQLDCVRVDAKFQPIPGTEFTLQADLVLLAMGFVSPVKQGLLEK
HHHHHHHHCCCCCCCEEEEEEEEECCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LGVALDPRGNVSADLSHFTTSVDKVFAAGDMRRGQSLVVWAIREGRLCARAIDQFLMGST
HCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCC
TLPK
CCCC
>Mature Secondary Structure 
GKITGFLEIERHDRVYAPVAERVKNYNEFVVPLSDKDLGDQAARCMNCGIPYCHGTGSA
CCEEEEEEEECCCCEEHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCC
QPGAPGCPVNNQIPDWNDLVYQGNWQEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDD
CCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEECC
NPVTIKTIECAIVDRAWENGWLVPELASVKTGQSVAVIGAGPAGLACAQQLARAGHDVHV
CCEEEEEEEEEEEECCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHH
YEKLAKAGGLLRYGIPDFKMEKHLIDRRVAQMEAEGVTFHYNAPVGGNAAGAVDPAELLK
HHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHH
QYDAVALTGGAEYPRDLPIPGRELSGIHFAMDFLTQQNRRVSGEAVNGADILATGKHVVV
HCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEE
IGGGDTGSDCIGTSIRQGATSVTQIEIMAAPPEKEDKGLTWPNWPMKMRTSSSQAEGAVR
EECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
EFAVLTQSFTGENGAVKQLDCVRVDAKFQPIPGTEFTLQADLVLLAMGFVSPVKQGLLEK
HHHHHHHHCCCCCCCEEEEEEEEECCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LGVALDPRGNVSADLSHFTTSVDKVFAAGDMRRGQSLVVWAIREGRLCARAIDQFLMGST
HCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCC
TLPK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]