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The map label for this gene is cheB

Identifier: 86751025

GI number: 86751025

Start: 4468960

End: 4470126

Strand: Reverse

Name: cheB

Synonym: RPB_3917

Alternate gene names: 86751025

Gene position: 4470126-4468960 (Counterclockwise)

Preceding gene: 86751026

Following gene: 86751024

Centisome position: 83.84

GC content: 69.15

Gene sequence:

>1167_bases
ATGAGTGTAGCTCTGGCAGGAAGTCCGGCCATCAATGCGACGCAGTATGAACCGCTGCGGGTGATGGTCGTCGACGATTC
GGTGGTGATTCGCGGGCTGATCTCGCGTTGGATCGAGGCCGAGCCCGACATGGTCGTCGCGGCCTCGCTGCGCACCGGGC
TCGACGCGGTCAATCAGGTCGAACGCATCAAGCCGGACATCGCCCTGCTCGACATCGAGATGCCCGAGCTCGACGGCATC
GCCGCGCTGCCGCAATTGCTGGCCAAGAAGCGCGACCTGATCGTCATCATGGCGTCGACCCTGACGCGCCGTAACGCCGA
GATCAGCTTCAAGGCGCTGTCGCTCGGCGCCGCCGACTACATTCCGAAGCCGGAGACGACCCGCGAATCCGCCGGCGCCG
AGGTGTTCAGGCACGACCTGCTGCAGAAGATCCGCCATCTCGGCGGCAAGGTGCGGCGCAGGGTCTCGGCGCCGAGCGCC
AGCGCGCCCATGGTCGCGCGCGTTCGCGAGGCGGCCGCAGCACCGGCCGCGCCGAGCGTCGCCAGTCAGGCGGCGCTGGT
GAAGCGTGCGTTCGGCCCGACGGCGCCGCGCGTGCTGCTGATCGGGTCCTCGACCGGCGGGCCGCAGGCGCTGATGTCGC
TGGTCGCCGAGATCGGTCCGGTGATCGACCGCTATCCGGTGCTGATCACGCAGCATATGCCGCCGACCTTCACGACGATT
CTCGCCGAACATCTGATGCGCGCCGCCCGGCGGCCGGCGCACGAGGGCGTCGACGGCGAACTGATCAAGCCGGGCCGGAT
CTATCTGGCCCCCGGCGGTCGCCACATGCGGGTGACCCGCCAGGGCGGACAGCCCGCGATCGCGCTCGACGACGGCCCGG
CGGTGAATTTCTGCAAGCCCGCGGTCGACCCGCTGTTCACCTCGGCGATCGACGTCTGGCAGGGCGGGATCATGGCGGTG
GTGTTGACCGGAATGGGGTCCGACGGCATGCGCGGCGGCAAGGACATCGTCGCCGCCGGCGGCAGCGTGATCGCCCAGGA
CGAGGCCAGCAGCGTGGTGTGGGGCATGCCCGGCGCGGCGGCGAATGCCGGCATCTGCGCCGCGGTGTTGCCGCTCAATC
AGATCGGGCCGAAGCTCGTACGTTTGTTTTCAGGAGATCGGTCGTGA

Upstream 100 bases:

>100_bases
GAAGTTCCAGGAAGTCGGCCTGATCTGACGGGGTCTGCCGGGATGTCGTCGACGCTGCGTCATTCGACGCGGCGGGGGTG
TCGTGTTGCGTCGGTGTGTG

Downstream 100 bases:

>100_bases
CGCCGCTGGACTACGAATATCTGCGCAAGGCGTTGAAGGACCGCTCCGGGCTCGATCTGTCGATCGACAAGCAGTACCTC
GTCGAAAGCCGGCTGCTGCC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSVALAGSPAINATQYEPLRVMVVDDSVVIRGLISRWIEAEPDMVVAASLRTGLDAVNQVERIKPDIALLDIEMPELDGI
AALPQLLAKKRDLIVIMASTLTRRNAEISFKALSLGAADYIPKPETTRESAGAEVFRHDLLQKIRHLGGKVRRRVSAPSA
SAPMVARVREAAAAPAAPSVASQAALVKRAFGPTAPRVLLIGSSTGGPQALMSLVAEIGPVIDRYPVLITQHMPPTFTTI
LAEHLMRAARRPAHEGVDGELIKPGRIYLAPGGRHMRVTRQGGQPAIALDDGPAVNFCKPAVDPLFTSAIDVWQGGIMAV
VLTGMGSDGMRGGKDIVAAGGSVIAQDEASSVVWGMPGAAANAGICAAVLPLNQIGPKLVRLFSGDRS

Sequences:

>Translated_388_residues
MSVALAGSPAINATQYEPLRVMVVDDSVVIRGLISRWIEAEPDMVVAASLRTGLDAVNQVERIKPDIALLDIEMPELDGI
AALPQLLAKKRDLIVIMASTLTRRNAEISFKALSLGAADYIPKPETTRESAGAEVFRHDLLQKIRHLGGKVRRRVSAPSA
SAPMVARVREAAAAPAAPSVASQAALVKRAFGPTAPRVLLIGSSTGGPQALMSLVAEIGPVIDRYPVLITQHMPPTFTTI
LAEHLMRAARRPAHEGVDGELIKPGRIYLAPGGRHMRVTRQGGQPAIALDDGPAVNFCKPAVDPLFTSAIDVWQGGIMAV
VLTGMGSDGMRGGKDIVAAGGSVIAQDEASSVVWGMPGAAANAGICAAVLPLNQIGPKLVRLFSGDRS
>Mature_387_residues
SVALAGSPAINATQYEPLRVMVVDDSVVIRGLISRWIEAEPDMVVAASLRTGLDAVNQVERIKPDIALLDIEMPELDGIA
ALPQLLAKKRDLIVIMASTLTRRNAEISFKALSLGAADYIPKPETTRESAGAEVFRHDLLQKIRHLGGKVRRRVSAPSAS
APMVARVREAAAAPAAPSVASQAALVKRAFGPTAPRVLLIGSSTGGPQALMSLVAEIGPVIDRYPVLITQHMPPTFTTIL
AEHLMRAARRPAHEGVDGELIKPGRIYLAPGGRHMRVTRQGGQPAIALDDGPAVNFCKPAVDPLFTSAIDVWQGGIMAVV
LTGMGSDGMRGGKDIVAAGGSVIAQDEASSVVWGMPGAAANAGICAAVLPLNQIGPKLVRLFSGDRS

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=364, Percent_Identity=34.8901098901099, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_RHOP2 (Q2IT50)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_487521.1
- ProteinModelPortal:   Q2IT50
- SMR:   Q2IT50
- STRING:   Q2IT50
- GeneID:   3911722
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3917
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   QVVMMST
- ProtClustDB:   PRK00742
- BioCyc:   RPAL316058:RPB_3917-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 40740; Mature: 40609

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 204-204 ACT_SITE 232-232 ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVALAGSPAINATQYEPLRVMVVDDSVVIRGLISRWIEAEPDMVVAASLRTGLDAVNQV
CCEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
ERIKPDIALLDIEMPELDGIAALPQLLAKKRDLIVIMASTLTRRNAEISFKALSLGAADY
HHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCCEEEEEEECCCHHC
IPKPETTRESAGAEVFRHDLLQKIRHLGGKVRRRVSAPSASAPMVARVREAAAAPAAPSV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHH
ASQAALVKRAFGPTAPRVLLIGSSTGGPQALMSLVAEIGPVIDRYPVLITQHMPPTFTTI
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHH
LAEHLMRAARRPAHEGVDGELIKPGRIYLAPGGRHMRVTRQGGQPAIALDDGPAVNFCKP
HHHHHHHHHCCCCCCCCCCCEECCCEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCHH
AVDPLFTSAIDVWQGGIMAVVLTGMGSDGMRGGKDIVAAGGSVIAQDEASSVVWGMPGAA
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEECCCCEEECCCCCCEEECCCCCC
ANAGICAAVLPLNQIGPKLVRLFSGDRS
CCCCCEEEECCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVALAGSPAINATQYEPLRVMVVDDSVVIRGLISRWIEAEPDMVVAASLRTGLDAVNQV
CEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
ERIKPDIALLDIEMPELDGIAALPQLLAKKRDLIVIMASTLTRRNAEISFKALSLGAADY
HHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCCEEEEEEECCCHHC
IPKPETTRESAGAEVFRHDLLQKIRHLGGKVRRRVSAPSASAPMVARVREAAAAPAAPSV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHH
ASQAALVKRAFGPTAPRVLLIGSSTGGPQALMSLVAEIGPVIDRYPVLITQHMPPTFTTI
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHH
LAEHLMRAARRPAHEGVDGELIKPGRIYLAPGGRHMRVTRQGGQPAIALDDGPAVNFCKP
HHHHHHHHHCCCCCCCCCCCEECCCEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCHH
AVDPLFTSAIDVWQGGIMAVVLTGMGSDGMRGGKDIVAAGGSVIAQDEASSVVWGMPGAA
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEECCCCEEECCCCCCEEECCCCCC
ANAGICAAVLPLNQIGPKLVRLFSGDRS
CCCCCEEEECCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA