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The map label for this gene is sigK [H]

Identifier: 86751017

GI number: 86751017

Start: 4462199

End: 4462747

Strand: Reverse

Name: sigK [H]

Synonym: RPB_3909

Alternate gene names: 86751017

Gene position: 4462747-4462199 (Counterclockwise)

Preceding gene: 86751018

Following gene: 86751016

Centisome position: 83.7

GC content: 63.21

Gene sequence:

>549_bases
ATGCTGACGTCGGCTGAGTTGGTCTGGCTGCTTGCCGCTGTGGCCAAAGGGGATCAGGCAGCCTTCGAGCGGCTCTACAA
CGCCACGCGCGCGAAACTCTACGGCGTGGTTCTGCGTATCTTGAGACGTCAGGATCTCGCGGAAGAAGTCATCCAGGAAA
CGTACGTCAAGATCTGGAACAGCGCCGGAACGTTCAATCCCGGCGTGGCGTCGCCGATCACCTGGATGGCGTCGATCGCG
CGGAACCGGGCCATCGACGTGGCGCGCAAGCGCAGCGAGGTTTCGATCGAGGACGAGCCTGCGGCGCAGGAAGCCGCGGC
GGATTCGCCGGATCCGCTGGCGCGCCGGGAGATGACTGAAGAATTGAAGCGGCTGCTCGAATGCGTCGGCCGGCTGGAGC
CGGATCGCCAGAAGCTCGTGCTGCTCGCTTACTACAATGGTTGGAGCCGCGAACAGCTCGCGGCGAAATTCGATGCGCCG
GTGAACACGGTCAAGACTTGGCTGCGTCGCAGCCTGCTCGATATCCGGGAGTGTCTGGGACTCGTCTGA

Upstream 100 bases:

>100_bases
TGCGACATGGCGGACCGAAACGTCGCGAGATATGCTACGAACGGCCGACGCGCTCGACCGGACGGTCGGGGCGGCGCGGA
ATTGCCGGGATGGGGACTGA

Downstream 100 bases:

>100_bases
TGGCCATTAGCGAAGACCATATCGCGCTCGCCGCGGAATACGCGCTCGGTACGCTCGATGCCGGCGAGCGGGCGCAGGTC
GAGTCCATGATGGCTGCCGA

Product: sigma-24 (FecI-like)

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MLTSAELVWLLAAVAKGDQAAFERLYNATRAKLYGVVLRILRRQDLAEEVIQETYVKIWNSAGTFNPGVASPITWMASIA
RNRAIDVARKRSEVSIEDEPAAQEAAADSPDPLARREMTEELKRLLECVGRLEPDRQKLVLLAYYNGWSREQLAAKFDAP
VNTVKTWLRRSLLDIRECLGLV

Sequences:

>Translated_182_residues
MLTSAELVWLLAAVAKGDQAAFERLYNATRAKLYGVVLRILRRQDLAEEVIQETYVKIWNSAGTFNPGVASPITWMASIA
RNRAIDVARKRSEVSIEDEPAAQEAAADSPDPLARREMTEELKRLLECVGRLEPDRQKLVLLAYYNGWSREQLAAKFDAP
VNTVKTWLRRSLLDIRECLGLV
>Mature_182_residues
MLTSAELVWLLAAVAKGDQAAFERLYNATRAKLYGVVLRILRRQDLAEEVIQETYVKIWNSAGTFNPGVASPITWMASIA
RNRAIDVARKRSEVSIEDEPAAQEAAADSPDPLARREMTEELKRLLECVGRLEPDRQKLVLLAYYNGWSREQLAAKFDAP
VNTVKTWLRRSLLDIRECLGLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 20543; Mature: 20543

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSAELVWLLAAVAKGDQAAFERLYNATRAKLYGVVLRILRRQDLAEEVIQETYVKIWN
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGTFNPGVASPITWMASIARNRAIDVARKRSEVSIEDEPAAQEAAADSPDPLARREMTE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
ELKRLLECVGRLEPDRQKLVLLAYYNGWSREQLAAKFDAPVNTVKTWLRRSLLDIRECLG
HHHHHHHHHHCCCCCCHHEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure
MLTSAELVWLLAAVAKGDQAAFERLYNATRAKLYGVVLRILRRQDLAEEVIQETYVKIWN
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGTFNPGVASPITWMASIARNRAIDVARKRSEVSIEDEPAAQEAAADSPDPLARREMTE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
ELKRLLECVGRLEPDRQKLVLLAYYNGWSREQLAAKFDAPVNTVKTWLRRSLLDIRECLG
HHHHHHHHHHCCCCCCHHEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA