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The map label for this gene is rffG [H]

Identifier: 86750919

GI number: 86750919

Start: 4347875

End: 4348912

Strand: Reverse

Name: rffG [H]

Synonym: RPB_3810

Alternate gene names: 86750919

Gene position: 4348912-4347875 (Counterclockwise)

Preceding gene: 86750920

Following gene: 86750918

Centisome position: 81.57

GC content: 65.99

Gene sequence:

>1038_bases
GTGACCCGACCGATCCCGGCATCCTCTCCCGACGCGAAGGCCGGCGCGGCGTTCGGCAAGGTGCTGGTGATCGGCAGCAA
TTCCTTCACCGGCGCCGAGATGGTGCGCGATCTGCTCGACCACGGCGCCGAAGTGGTCGGCGTCAGCCGCTCGCCGGAGC
CGCACCAGGCGTTCCTGCCGTATCGCTGGACCAACACCGAGCGGTTCCGCTTCCGCCAGCTCGATCTCAACCACGACCTC
GACGCGCTGATGGCGCTGATCGAGGCCGAGCGGTGTCCCTGGATCATCAATTTCGCCGCGCAAAGCATGGTCGGCGAGAG
CTGGCTCAATCCCGACCACTGGTTCATGACCAACGTGGTGTCCACTGTGCGATTGCACGAGCGGCTGCGGCACTGCGACT
TCCTCGATCGCTACGTCCACGTCACCACGCCGGAGGTCTACGGCAACGCCACCGGCACGCTGACCGAGGAGGCGCCGTTC
GATCCCTCGACGCCCTATGCGGTGTCGCGCGCGGCCGGCGACATGAGCCTGCGCTCGTATTTCCGCGCCTATCAGTTTCC
GGTGCTGTACACCCGCGCCGCCAATGTCTACGGGCCGGGGCAGCGGCTGTACCGGATCGTGCCGCGCACCATCTTGTTCA
TCCGATTGGGCCGCAAGCTGCAACTGCACGGCGGCGGCACCTCGGAACGCTCGTTCATCGCAGCTTCCGACGTCGCCGAC
GCGACGCGGCGGATCGCGGCCGCAGGCACGCTCGGCGACACCTACCACATCGCGACCGACCGCATCGTCACCATCCGCGA
GCTGGTCGAACTGATCTGCGCGACGATGGGCGTGAAGTTCGAGGATCACGTCGAGATCGTCGGCGAACGGCTCGGCAAGG
ACTCGGCTTACCGACTCGACAGCGGCAAGATCCGCCGTGAGCTCGGCTGGAGCGACCGCGTCACGCTGGAGCAGGGCATC
GACGATACCATCCGCTGGGTCGATCGCTGGTTCGATGTGTTGAAGGACCAACCTTTCGACTATGCGCACAAGCCTTGA

Upstream 100 bases:

>100_bases
ACGACGCGATCCGCGAGATCATCGTCAAGTTCCAGCGCGGCGAATTGAAGAACGAGGACCGGCACTACAATCTGCGCTGG
ATGCAGCGGGGCGTGCAGGC

Downstream 100 bases:

>100_bases
GGACATCGATGGGCCAGGAAATCGATCTGCTCGTCAACTACCCGCGCACCAAGCGCAATGTCGACGAGCGCGGCCAGACC
AAATCCGAAGAGGACCGCGC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTRPIPASSPDAKAGAAFGKVLVIGSNSFTGAEMVRDLLDHGAEVVGVSRSPEPHQAFLPYRWTNTERFRFRQLDLNHDL
DALMALIEAERCPWIINFAAQSMVGESWLNPDHWFMTNVVSTVRLHERLRHCDFLDRYVHVTTPEVYGNATGTLTEEAPF
DPSTPYAVSRAAGDMSLRSYFRAYQFPVLYTRAANVYGPGQRLYRIVPRTILFIRLGRKLQLHGGGTSERSFIAASDVAD
ATRRIAAAGTLGDTYHIATDRIVTIRELVELICATMGVKFEDHVEIVGERLGKDSAYRLDSGKIRRELGWSDRVTLEQGI
DDTIRWVDRWFDVLKDQPFDYAHKP

Sequences:

>Translated_345_residues
MTRPIPASSPDAKAGAAFGKVLVIGSNSFTGAEMVRDLLDHGAEVVGVSRSPEPHQAFLPYRWTNTERFRFRQLDLNHDL
DALMALIEAERCPWIINFAAQSMVGESWLNPDHWFMTNVVSTVRLHERLRHCDFLDRYVHVTTPEVYGNATGTLTEEAPF
DPSTPYAVSRAAGDMSLRSYFRAYQFPVLYTRAANVYGPGQRLYRIVPRTILFIRLGRKLQLHGGGTSERSFIAASDVAD
ATRRIAAAGTLGDTYHIATDRIVTIRELVELICATMGVKFEDHVEIVGERLGKDSAYRLDSGKIRRELGWSDRVTLEQGI
DDTIRWVDRWFDVLKDQPFDYAHKP
>Mature_344_residues
TRPIPASSPDAKAGAAFGKVLVIGSNSFTGAEMVRDLLDHGAEVVGVSRSPEPHQAFLPYRWTNTERFRFRQLDLNHDLD
ALMALIEAERCPWIINFAAQSMVGESWLNPDHWFMTNVVSTVRLHERLRHCDFLDRYVHVTTPEVYGNATGTLTEEAPFD
PSTPYAVSRAAGDMSLRSYFRAYQFPVLYTRAANVYGPGQRLYRIVPRTILFIRLGRKLQLHGGGTSERSFIAASDVADA
TRRIAAAGTLGDTYHIATDRIVTIRELVELICATMGVKFEDHVEIVGERLGKDSAYRLDSGKIRRELGWSDRVTLEQGID
DTIRWVDRWFDVLKDQPFDYAHKP

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=30.9090909090909, Blast_Score=152, Evalue=3e-37,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=29.8550724637681, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=30.3977272727273, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17568069, Length=270, Percent_Identity=30.7407407407407, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532424, Length=273, Percent_Identity=25.2747252747253, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI133901786, Length=354, Percent_Identity=24.8587570621469, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=354, Percent_Identity=24.8587570621469, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=354, Percent_Identity=24.8587570621469, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=354, Percent_Identity=24.8587570621469, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=354, Percent_Identity=24.8587570621469, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=337, Percent_Identity=24.6290801186944, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=23.0985915492958, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38990; Mature: 38858

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIPASSPDAKAGAAFGKVLVIGSNSFTGAEMVRDLLDHGAEVVGVSRSPEPHQAFLP
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
YRWTNTERFRFRQLDLNHDLDALMALIEAERCPWIINFAAQSMVGESWLNPDHWFMTNVV
EEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHHHH
STVRLHERLRHCDFLDRYVHVTTPEVYGNATGTLTEEAPFDPSTPYAVSRAAGDMSLRSY
HHHHHHHHHHHHHHHHCEEEEECCHHHCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHH
FRAYQFPVLYTRAANVYGPGQRLYRIVPRTILFIRLGRKLQLHGGGTSERSFIAASDVAD
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHHH
ATRRIAAAGTLGDTYHIATDRIVTIRELVELICATMGVKFEDHVEIVGERLGKDSAYRLD
HHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECC
SGKIRRELGWSDRVTLEQGIDDTIRWVDRWFDVLKDQPFDYAHKP
CCHHHHHCCCCCCEEHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TRPIPASSPDAKAGAAFGKVLVIGSNSFTGAEMVRDLLDHGAEVVGVSRSPEPHQAFLP
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
YRWTNTERFRFRQLDLNHDLDALMALIEAERCPWIINFAAQSMVGESWLNPDHWFMTNVV
EEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHHHH
STVRLHERLRHCDFLDRYVHVTTPEVYGNATGTLTEEAPFDPSTPYAVSRAAGDMSLRSY
HHHHHHHHHHHHHHHHCEEEEECCHHHCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHH
FRAYQFPVLYTRAANVYGPGQRLYRIVPRTILFIRLGRKLQLHGGGTSERSFIAASDVAD
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHHH
ATRRIAAAGTLGDTYHIATDRIVTIRELVELICATMGVKFEDHVEIVGERLGKDSAYRLD
HHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEECC
SGKIRRELGWSDRVTLEQGIDDTIRWVDRWFDVLKDQPFDYAHKP
CCHHHHHCCCCCCEEHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]