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The map label for this gene is ilvB [H]

Identifier: 86750911

GI number: 86750911

Start: 4338928

End: 4340730

Strand: Reverse

Name: ilvB [H]

Synonym: RPB_3802

Alternate gene names: 86750911

Gene position: 4340730-4338928 (Counterclockwise)

Preceding gene: 86750912

Following gene: 86750909

Centisome position: 81.41

GC content: 67.67

Gene sequence:

>1803_bases
GTGAAGCTCTCCGACTACGTCATCGACTTCCTCGCGCAGCGCGGCGTCAGCCATGTGTTCGGCATTTCCGGCGGCGCGGC
GGTGCATATGTTCGATTCGGCGGCGAAGCATCCGGATGTCACGCCGATCTTCCCGCAGCACGAGCAGGCCGCCGCGATCG
CCGCCGACGGCTACGCGCGCGCCACCGGCAAGCTCGGCGTCGCCATCACCACCTCCGGCCCCGGCGCGACCAATCTGCTG
ACCGGGGTGTGCTGCGCGTATTATGATTCAGTGCCGACGCTGATGATCACCGGGCAGGTCGCGACGCATCGGCTCAAGGG
CAACAACGACGTCCGCCAGCTAGGCTTCCAGGAGACCGACGTGACGTCGATCTTCGCCACGGTGACGAAATATGCGGTGC
AGATCTCCGATCCCGCGACGATCCGCTATCATCTGGAAAAGGCCTACTATCTCGCCTTCGAGGGCCGGCCCGGCGCGGTG
CTGATCGATCTGCCGGACGATCTGCAGCGCGCCGAGATCGATCCGGAGGCGCTGGCGTCGTTCGTGCCGGAGACGCAGAT
CGCCACGACCGATCTCGACGCCGAGATCGTCGCCTTGCTGCCGCTGATCGCGCAGGCGAAGCGACCGGTGCTGGTGCTGG
GCGGCGGGCTGTCGACGCCGCGGATCGGCGCCGCGCTCGATCAACTGATCGACCGGCTCGCCATGCCGGTGCTGACGACC
TGGGCCGCGACCGATCTGATCGCGCATGATCATCCGCTGCGGGTCGGGCCGTTCGGTGTTTACGGGCCGCGGCTCGGCAA
TTTCACCGTGCAGAATGCCGACCTCATTCTCTGCCTCGGCAGCCGGCTGTCGCAGAACGTCACCGGCGGCATCCTGCCGT
CGTTCGCGCGCGAGGCGACGATCGTGATGGTCGACGCCAGCCGCGGCGAGATGGACAAGTTCGACGCGCGCGGCATCGCC
GTCGCGACGCGGATCGAGGCGCGGCTCGACGGGTTCGTGCCGAAGCTGCTCGGAGCGATCGAGGCCGCGCCGCCGCGCGA
CGAATGGCTGGCGCAGATCGCGCATTGGCGTAGCGCGCTGCCGGACGATCGTCCCGGTCCCGCGCCCGCCAATGCAGGCT
TCGTGGACGCCTACGACTTCGTCGACAAGTTGAGCGAGACCGCGCCCGCCGACGAGCTGATCTATGTCGACACCGGCGGC
AATCTGACCTGGACCTGCAACGGCTTCCGCATCCAGCGCGGCCAGCGGCTGATCTCCGACTGGAACAACACCGCGATGGG
CTATGCGCTGCCGGCCGCGATCGGCGCGGCGGTGCAGGCGAAGGGCGGGGTGAGCTGCATCATCGGCGATGGCGGCCTGA
TGCTGTCGCTCGGCGAACTGGCGCTGCTGTCGCGTCACAGGCTGCCGGTGCGGCTGTTCCTGTTCAACAATCACGGGCAC
GGCATCCAGAAGCAGACATTGGAGACCTGGCTCGACGGCAACTATGTCGGCGTCGATGCGCCGAGCGGATTGTCGTTCGT
CGATGTCGCCAAGGTCGCCGAGGCGATGGACCTGCCGGTGGTCACGATCAGCCGCAGCGCGGACATCGCCGCCAAGCTGC
GCGAGGTCTATGCGCGGCAGGGACCGGTGTTCTGCAATGTCGAGATCAATCCCGCGCAGAAGCTGTATCCGGTGCTGAAA
TTCGGCGCGCCGCTGGAAAGCCAGATGCCGGCGATCGACGACGCGCTGATCGCGCGCGAGATGATCGTGCCGCCGTTCGT
CCCCGGCGCGGCGCCGAAGCACAGCGGCGGCGCGGGGGTGTGA

Upstream 100 bases:

>100_bases
GGCGGCGCGACGCTGGCCGCGCGGCTCGAAGCCGACGGCAACATCTACAAGCTGCGGCGGCACGGCAATTGAGCGCGCCC
GCCCGCATCAGGAGGTTCGC

Downstream 100 bases:

>100_bases
GGCTGCGGAATGCTGAGTGTATTGCGTGTTCGCTTACTTGCTCGGCTCATACTGAATCGCCACTCGCTCTACCGTCATCC
TGAGGCGCGAGCGCAGCGAG

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKLSDYVIDFLAQRGVSHVFGISGGAAVHMFDSAAKHPDVTPIFPQHEQAAAIAADGYARATGKLGVAITTSGPGATNLL
TGVCCAYYDSVPTLMITGQVATHRLKGNNDVRQLGFQETDVTSIFATVTKYAVQISDPATIRYHLEKAYYLAFEGRPGAV
LIDLPDDLQRAEIDPEALASFVPETQIATTDLDAEIVALLPLIAQAKRPVLVLGGGLSTPRIGAALDQLIDRLAMPVLTT
WAATDLIAHDHPLRVGPFGVYGPRLGNFTVQNADLILCLGSRLSQNVTGGILPSFAREATIVMVDASRGEMDKFDARGIA
VATRIEARLDGFVPKLLGAIEAAPPRDEWLAQIAHWRSALPDDRPGPAPANAGFVDAYDFVDKLSETAPADELIYVDTGG
NLTWTCNGFRIQRGQRLISDWNNTAMGYALPAAIGAAVQAKGGVSCIIGDGGLMLSLGELALLSRHRLPVRLFLFNNHGH
GIQKQTLETWLDGNYVGVDAPSGLSFVDVAKVAEAMDLPVVTISRSADIAAKLREVYARQGPVFCNVEINPAQKLYPVLK
FGAPLESQMPAIDDALIAREMIVPPFVPGAAPKHSGGAGV

Sequences:

>Translated_600_residues
MKLSDYVIDFLAQRGVSHVFGISGGAAVHMFDSAAKHPDVTPIFPQHEQAAAIAADGYARATGKLGVAITTSGPGATNLL
TGVCCAYYDSVPTLMITGQVATHRLKGNNDVRQLGFQETDVTSIFATVTKYAVQISDPATIRYHLEKAYYLAFEGRPGAV
LIDLPDDLQRAEIDPEALASFVPETQIATTDLDAEIVALLPLIAQAKRPVLVLGGGLSTPRIGAALDQLIDRLAMPVLTT
WAATDLIAHDHPLRVGPFGVYGPRLGNFTVQNADLILCLGSRLSQNVTGGILPSFAREATIVMVDASRGEMDKFDARGIA
VATRIEARLDGFVPKLLGAIEAAPPRDEWLAQIAHWRSALPDDRPGPAPANAGFVDAYDFVDKLSETAPADELIYVDTGG
NLTWTCNGFRIQRGQRLISDWNNTAMGYALPAAIGAAVQAKGGVSCIIGDGGLMLSLGELALLSRHRLPVRLFLFNNHGH
GIQKQTLETWLDGNYVGVDAPSGLSFVDVAKVAEAMDLPVVTISRSADIAAKLREVYARQGPVFCNVEINPAQKLYPVLK
FGAPLESQMPAIDDALIAREMIVPPFVPGAAPKHSGGAGV
>Mature_600_residues
MKLSDYVIDFLAQRGVSHVFGISGGAAVHMFDSAAKHPDVTPIFPQHEQAAAIAADGYARATGKLGVAITTSGPGATNLL
TGVCCAYYDSVPTLMITGQVATHRLKGNNDVRQLGFQETDVTSIFATVTKYAVQISDPATIRYHLEKAYYLAFEGRPGAV
LIDLPDDLQRAEIDPEALASFVPETQIATTDLDAEIVALLPLIAQAKRPVLVLGGGLSTPRIGAALDQLIDRLAMPVLTT
WAATDLIAHDHPLRVGPFGVYGPRLGNFTVQNADLILCLGSRLSQNVTGGILPSFAREATIVMVDASRGEMDKFDARGIA
VATRIEARLDGFVPKLLGAIEAAPPRDEWLAQIAHWRSALPDDRPGPAPANAGFVDAYDFVDKLSETAPADELIYVDTGG
NLTWTCNGFRIQRGQRLISDWNNTAMGYALPAAIGAAVQAKGGVSCIIGDGGLMLSLGELALLSRHRLPVRLFLFNNHGH
GIQKQTLETWLDGNYVGVDAPSGLSFVDVAKVAEAMDLPVVTISRSADIAAKLREVYARQGPVFCNVEINPAQKLYPVLK
FGAPLESQMPAIDDALIAREMIVPPFVPGAAPKHSGGAGV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=487, Percent_Identity=24.0246406570842, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI21361361, Length=562, Percent_Identity=23.6654804270463, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=30.6737588652482, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=29.8951048951049, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=28.3393501805054, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=23.3273056057866, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788716, Length=566, Percent_Identity=23.8515901060071, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=489, Percent_Identity=25.3578732106339, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=489, Percent_Identity=25.3578732106339, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17542570, Length=513, Percent_Identity=25.1461988304094, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=29.3015332197615, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6320123, Length=548, Percent_Identity=23.3576642335766, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=510, Percent_Identity=26.4705882352941, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=516, Percent_Identity=24.6124031007752, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323163, Length=510, Percent_Identity=25.8823529411765, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320816, Length=309, Percent_Identity=24.9190938511327, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922626, Length=516, Percent_Identity=25.968992248062, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63852; Mature: 63852

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDYVIDFLAQRGVSHVFGISGGAAVHMFDSAAKHPDVTPIFPQHEQAAAIAADGYAR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCCEEECCCCCC
ATGKLGVAITTSGPGATNLLTGVCCAYYDSVPTLMITGQVATHRLKGNNDVRQLGFQETD
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHEEEECCCCHHHHCCCCHHH
VTSIFATVTKYAVQISDPATIRYHLEKAYYLAFEGRPGAVLIDLPDDLQRAEIDPEALAS
HHHHHHHHHHHEEEECCCCEEEEEEEEEEEEEEECCCCEEEEECCCCHHHCCCCHHHHHH
FVPETQIATTDLDAEIVALLPLIAQAKRPVLVLGGGLSTPRIGAALDQLIDRLAMPVLTT
HCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
WAATDLIAHDHPLRVGPFGVYGPRLGNFTVQNADLILCLGSRLSQNVTGGILPSFAREAT
HHHHHHHCCCCCCEECCCCCCCCCCCCEEECCCCEEEEECCHHCCCCCCCCCHHHCCCEE
IVMVDASRGEMDKFDARGIAVATRIEARLDGFVPKLLGAIEAAPPRDEWLAQIAHWRSAL
EEEEECCCCCCCHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
PDDRPGPAPANAGFVDAYDFVDKLSETAPADELIYVDTGGNLTWTCNGFRIQRGQRLISD
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCEEEECCHHHHHC
WNNTAMGYALPAAIGAAVQAKGGVSCIIGDGGLMLSLGELALLSRHRLPVRLFLFNNHGH
CCCCCCCCHHHHHHHHHEECCCCEEEEEECCCEEEEHHHHHHHHCCCCCEEEEEECCCCC
GIQKQTLETWLDGNYVGVDAPSGLSFVDVAKVAEAMDLPVVTISRSADIAAKLREVYARQ
CCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHC
GPVFCNVEINPAQKLYPVLKFGAPLESQMPAIDDALIAREMIVPPFVPGAAPKHSGGAGV
CCEEEEEEECCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLSDYVIDFLAQRGVSHVFGISGGAAVHMFDSAAKHPDVTPIFPQHEQAAAIAADGYAR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCCEEECCCCCC
ATGKLGVAITTSGPGATNLLTGVCCAYYDSVPTLMITGQVATHRLKGNNDVRQLGFQETD
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHEEEECCCCHHHHCCCCHHH
VTSIFATVTKYAVQISDPATIRYHLEKAYYLAFEGRPGAVLIDLPDDLQRAEIDPEALAS
HHHHHHHHHHHEEEECCCCEEEEEEEEEEEEEEECCCCEEEEECCCCHHHCCCCHHHHHH
FVPETQIATTDLDAEIVALLPLIAQAKRPVLVLGGGLSTPRIGAALDQLIDRLAMPVLTT
HCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
WAATDLIAHDHPLRVGPFGVYGPRLGNFTVQNADLILCLGSRLSQNVTGGILPSFAREAT
HHHHHHHCCCCCCEECCCCCCCCCCCCEEECCCCEEEEECCHHCCCCCCCCCHHHCCCEE
IVMVDASRGEMDKFDARGIAVATRIEARLDGFVPKLLGAIEAAPPRDEWLAQIAHWRSAL
EEEEECCCCCCCHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
PDDRPGPAPANAGFVDAYDFVDKLSETAPADELIYVDTGGNLTWTCNGFRIQRGQRLISD
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCEEEECCHHHHHC
WNNTAMGYALPAAIGAAVQAKGGVSCIIGDGGLMLSLGELALLSRHRLPVRLFLFNNHGH
CCCCCCCCHHHHHHHHHEECCCCEEEEEECCCEEEEHHHHHHHHCCCCCEEEEEECCCCC
GIQKQTLETWLDGNYVGVDAPSGLSFVDVAKVAEAMDLPVVTISRSADIAAKLREVYARQ
CCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHC
GPVFCNVEINPAQKLYPVLKFGAPLESQMPAIDDALIAREMIVPPFVPGAAPKHSGGAGV
CCEEEEEEECCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]