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The map label for this gene is fliM [H]

Identifier: 86750893

GI number: 86750893

Start: 4319698

End: 4320903

Strand: Reverse

Name: fliM [H]

Synonym: RPB_3784

Alternate gene names: 86750893

Gene position: 4320903-4319698 (Counterclockwise)

Preceding gene: 86750894

Following gene: 86750892

Centisome position: 81.04

GC content: 64.43

Gene sequence:

>1206_bases
ATGGCTGGCCAGGAACAACTCGATCAAGACGCCATCGCCGCCCAATGGGAAGCGTCGATGGCGTCGGAAGACCCCGAGGC
CGCCGCGAAGGCGGCCGAGGAGAACGAACTCACCGGAACCATGGCGGAACAATGGGCCGCCATGGTCGAGGACGGCGGTC
GCGATCTCGGCGGCACCGGCAAGAACAGCGGCGAGCGCGTGTTGTCGCAGGAGGAGATCGACAATCTCCTCGGCTTCAGC
GTCGGCGAGGTGCATCTCGACGAGAACTCCGGCATCCGCGCGATCATCGATTCGGCGATGGTCTCCTACGAGCGCTTGCC
GATGCTCGAAATCGTGTTCGACCGCTTGGTGCGGTTGATGACGACGAGCCTGCGCAATTTCACCTCCGACAACGTCGAAG
TCTCGCTCGACCGCATCACCTCGGTGCGCTTCAGCGACTACATGAACTCGATCCCGCTGCCGGCGGTGCTGTGCGTGTTC
AAGGCCGAGGAGTGGCAGAATTTCGGATTGGCCACGGTCGATTCGAGCCTGATCTATTCGATGATCGACGTGCTGCTCGG
CGGCCGGCGCGGCCAGGCCGCGATCCGGATCGAGGGTCGGCCCTACACCACGATCGAAACCAATCTGGTGAAGCGGTTGC
TGGAAGTCGTGCTCGCCGACGCCGAGCAGGCGTTCCGGCCGCTGTCGCCGGTGGCGTTCTCGATCGACCGGCTCGAAACC
AATCCGCGCTTCGCCGCGATCAGCCGCCCGGCCAATGCGGCGATCCTGGTCCGTCTGCACATCGACATGGAAGACCGCGG
CGGCAATATCGAGCTGCTGTTGCCTTACGCCACGATCGAACCGATCCGCGGCGTGCTGATGCAGATGTTCATGGGCGAGA
AATTCGGCCGTGACCAGGTCTGGGAGGGCCATCTCGCCACCGAGATCGGCCAGGCCGACATTTCCGTCGACGCCGTATTG
TACGAGGCGGAGGTGCCGCTGCGGCAGTTGATGGCGCTGCAGGTCGGCGACACGCTGCCGCTGGATCTGCGCGCCGACGC
GCTGGTCGCGGTGCGCTGCGGCAGCGTCACGCTGACGGAAGGACGAATGGGCCGGGTCGGCGATCGCGTCGCCATCCGCG
TCACCAAACCATTGCGCAAGCCGGTCACGACCTACGCGATGTTCGAGCGGACCGACGAGCAAAGCAAAATGATGGAGGCA
CAATGA

Upstream 100 bases:

>100_bases
CCGGATGAAGGAAGAACTGACCCGACGCGTCAACGTCGCGATCGCGCCGGCGCAGGTGACAGCGGTGCTGTTCAAGGAAG
TACTGGTGCAGTGAGCTGAC

Downstream 100 bases:

>100_bases
GCCATACTCTCGGACTGGTCATCGAAAGTCTGGTCGCTGTGCTGCTGATCCTGACGATCGGCTATTGCTGGCTGCTCAAC
AAGCGGCTGCTCCGCCTCAG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAGQEQLDQDAIAAQWEASMASEDPEAAAKAAEENELTGTMAEQWAAMVEDGGRDLGGTGKNSGERVLSQEEIDNLLGFS
VGEVHLDENSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFSDYMNSIPLPAVLCVF
KAEEWQNFGLATVDSSLIYSMIDVLLGGRRGQAAIRIEGRPYTTIETNLVKRLLEVVLADAEQAFRPLSPVAFSIDRLET
NPRFAAISRPANAAILVRLHIDMEDRGGNIELLLPYATIEPIRGVLMQMFMGEKFGRDQVWEGHLATEIGQADISVDAVL
YEAEVPLRQLMALQVGDTLPLDLRADALVAVRCGSVTLTEGRMGRVGDRVAIRVTKPLRKPVTTYAMFERTDEQSKMMEA
Q

Sequences:

>Translated_401_residues
MAGQEQLDQDAIAAQWEASMASEDPEAAAKAAEENELTGTMAEQWAAMVEDGGRDLGGTGKNSGERVLSQEEIDNLLGFS
VGEVHLDENSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFSDYMNSIPLPAVLCVF
KAEEWQNFGLATVDSSLIYSMIDVLLGGRRGQAAIRIEGRPYTTIETNLVKRLLEVVLADAEQAFRPLSPVAFSIDRLET
NPRFAAISRPANAAILVRLHIDMEDRGGNIELLLPYATIEPIRGVLMQMFMGEKFGRDQVWEGHLATEIGQADISVDAVL
YEAEVPLRQLMALQVGDTLPLDLRADALVAVRCGSVTLTEGRMGRVGDRVAIRVTKPLRKPVTTYAMFERTDEQSKMMEA
Q
>Mature_400_residues
AGQEQLDQDAIAAQWEASMASEDPEAAAKAAEENELTGTMAEQWAAMVEDGGRDLGGTGKNSGERVLSQEEIDNLLGFSV
GEVHLDENSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFSDYMNSIPLPAVLCVFK
AEEWQNFGLATVDSSLIYSMIDVLLGGRRGQAAIRIEGRPYTTIETNLVKRLLEVVLADAEQAFRPLSPVAFSIDRLETN
PRFAAISRPANAAILVRLHIDMEDRGGNIELLLPYATIEPIRGVLMQMFMGEKFGRDQVWEGHLATEIGQADISVDAVLY
EAEVPLRQLMALQVGDTLPLDLRADALVAVRCGSVTLTEGRMGRVGDRVAIRVTKPLRKPVTTYAMFERTDEQSKMMEAQ

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=322, Percent_Identity=24.5341614906832, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 44271; Mature: 44140

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQEQLDQDAIAAQWEASMASEDPEAAAKAAEENELTGTMAEQWAAMVEDGGRDLGGTG
CCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
KNSGERVLSQEEIDNLLGFSVGEVHLDENSGIRAIIDSAMVSYERLPMLEIVFDRLVRLM
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TTSLRNFTSDNVEVSLDRITSVRFSDYMNSIPLPAVLCVFKAEEWQNFGLATVDSSLIYS
HHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MIDVLLGGRRGQAAIRIEGRPYTTIETNLVKRLLEVVLADAEQAFRPLSPVAFSIDRLET
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHCC
NPRFAAISRPANAAILVRLHIDMEDRGGNIELLLPYATIEPIRGVLMQMFMGEKFGRDQV
CCCEEEECCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC
WEGHLATEIGQADISVDAVLYEAEVPLRQLMALQVGDTLPLDLRADALVAVRCGSVTLTE
CCCHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCEEEEC
GRMGRVGDRVAIRVTKPLRKPVTTYAMFERTDEQSKMMEAQ
CCCCCCCCEEEEEECCHHHCCHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
AGQEQLDQDAIAAQWEASMASEDPEAAAKAAEENELTGTMAEQWAAMVEDGGRDLGGTG
CCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
KNSGERVLSQEEIDNLLGFSVGEVHLDENSGIRAIIDSAMVSYERLPMLEIVFDRLVRLM
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TTSLRNFTSDNVEVSLDRITSVRFSDYMNSIPLPAVLCVFKAEEWQNFGLATVDSSLIYS
HHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MIDVLLGGRRGQAAIRIEGRPYTTIETNLVKRLLEVVLADAEQAFRPLSPVAFSIDRLET
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHCC
NPRFAAISRPANAAILVRLHIDMEDRGGNIELLLPYATIEPIRGVLMQMFMGEKFGRDQV
CCCEEEECCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC
WEGHLATEIGQADISVDAVLYEAEVPLRQLMALQVGDTLPLDLRADALVAVRCGSVTLTE
CCCHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCEEEEC
GRMGRVGDRVAIRVTKPLRKPVTTYAMFERTDEQSKMMEAQ
CCCCCCCCEEEEEECCHHHCCHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1315735 [H]