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The map label for this gene is trmJ [H]

Identifier: 86750840

GI number: 86750840

Start: 4265545

End: 4266741

Strand: Reverse

Name: trmJ [H]

Synonym: RPB_3731

Alternate gene names: 86750840

Gene position: 4266741-4265545 (Counterclockwise)

Preceding gene: 86750842

Following gene: 86750839

Centisome position: 80.03

GC content: 69.51

Gene sequence:

>1197_bases
ATGGCCGGTTCCGGCACCGATAAATCCAAGCCCGCTGCGGACCTGGCCGGTCCCGTTGTGATTCTCGTTGAACCTCAACT
GGGCGAAAATATCGGGATGTGCGCCCGCGCCATGGGTAATTTCGGCCTGAAACGTTTACGTCTGGTGAAGCCGCGCGACG
GCTGGCCCAATGTCCATGCCCGCCGCTCGGCCTCCGGCGCCGATCACATCCTGGACGCGGTCGAACTGTTCGAGACGGTG
GGCGAGGCGGTGGCCGACTGCACGCTGCTGTTCGCCACCACCGCGCGCGCGCATGATCAGGCCAAGCCGGTGCGAGGGCC
GGAGGCGGCGGCGCAGGAGATGATCACCGCGGCCGTGGGCGGCGGCACCGCCGGCATCCTGTTCGGCCGCGAGCGCTACG
GCCTCGAGAACCACGAGGTGGCGCTCGCCAACCGCATCGTCACCTTCCCGGTCAACCCCGCCTTCGCCTCGCTGAACCTC
GCCCAGGCCGTGCTGCTGATGGGCTACGAGTGGTTCAAGCACGCCACCGGCGGCGCGCTGCCCTATGCGATGCCGGAGCG
CTCCGAGCCCGCCTCGCAGCACCAGATGACGGCGTTCTTCGACAACCTGGTCGCCGAGCTCGACCGCGTGGAATTTCTGC
GCCCGCCGGAGAAGCGCGACACCATGCTGGTCAATCTGCGCAACATCTTCACCCGGATGGACCCGACCAGGCAGGACATC
CACACGCTGCACGGCGCGGTGATGGCGATCGCCGAGGGCCGCAAGGGCCCAGCCAAGGGCGGCGTGCTCGACGGCGCGCA
GGCGACCAAGCTGCGCGCGCTGTTGGCCGAGCGCGCCGCCGCGGGCCATGGCGACGACGCCGGCTCGATGCGCGGGCTGG
CGCGAATGCTCCGCCGCAACCCGACCGACGCCGAGCGGCTGCTCTGGGAGCACCTGCGCAAGGACCGCCGCTTCGCCGGC
CAATTCAAGCGCCAGACCCCGGTCGGCCGTCACATCCCGGATTTCGTCTCGTTCCCCCACCGCATCGCGATCGAGCTGGT
CAATCCGGACGAAAGCGACGCGATCGCGACCGACCGCGCGGCGCGCAAGGACTGGCTGGAAGCGCGCGACTATCGCGTCG
TGCTGATCGAGGCGGCGATGGTCGAGCGGGATATCGCCGAGGTGCTGGGGCGGCTGGAGGCGGTGGTGAAGGGGTAG

Upstream 100 bases:

>100_bases
AGGCCGCCGCGGCCGCCTTGTTCAAGCTCCGCTATAATCTCAACACGCGCATACGGCGCGCCTTGGTCGAACCCGAGGCG
GTTCTTTGAAAGCTCGTTTG

Downstream 100 bases:

>100_bases
GGCGTCATCCGGGCGGTCTTGTGAGGGCCGGCGCGATGGCATATGTTAATGGCATATGCCATGGAGGTCAGCATGCCGGT
CCACCGACACGTCCGGTTGT

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAGSGTDKSKPAADLAGPVVILVEPQLGENIGMCARAMGNFGLKRLRLVKPRDGWPNVHARRSASGADHILDAVELFETV
GEAVADCTLLFATTARAHDQAKPVRGPEAAAQEMITAAVGGGTAGILFGRERYGLENHEVALANRIVTFPVNPAFASLNL
AQAVLLMGYEWFKHATGGALPYAMPERSEPASQHQMTAFFDNLVAELDRVEFLRPPEKRDTMLVNLRNIFTRMDPTRQDI
HTLHGAVMAIAEGRKGPAKGGVLDGAQATKLRALLAERAAAGHGDDAGSMRGLARMLRRNPTDAERLLWEHLRKDRRFAG
QFKRQTPVGRHIPDFVSFPHRIAIELVNPDESDAIATDRAARKDWLEARDYRVVLIEAAMVERDIAEVLGRLEAVVKG

Sequences:

>Translated_398_residues
MAGSGTDKSKPAADLAGPVVILVEPQLGENIGMCARAMGNFGLKRLRLVKPRDGWPNVHARRSASGADHILDAVELFETV
GEAVADCTLLFATTARAHDQAKPVRGPEAAAQEMITAAVGGGTAGILFGRERYGLENHEVALANRIVTFPVNPAFASLNL
AQAVLLMGYEWFKHATGGALPYAMPERSEPASQHQMTAFFDNLVAELDRVEFLRPPEKRDTMLVNLRNIFTRMDPTRQDI
HTLHGAVMAIAEGRKGPAKGGVLDGAQATKLRALLAERAAAGHGDDAGSMRGLARMLRRNPTDAERLLWEHLRKDRRFAG
QFKRQTPVGRHIPDFVSFPHRIAIELVNPDESDAIATDRAARKDWLEARDYRVVLIEAAMVERDIAEVLGRLEAVVKG
>Mature_397_residues
AGSGTDKSKPAADLAGPVVILVEPQLGENIGMCARAMGNFGLKRLRLVKPRDGWPNVHARRSASGADHILDAVELFETVG
EAVADCTLLFATTARAHDQAKPVRGPEAAAQEMITAAVGGGTAGILFGRERYGLENHEVALANRIVTFPVNPAFASLNLA
QAVLLMGYEWFKHATGGALPYAMPERSEPASQHQMTAFFDNLVAELDRVEFLRPPEKRDTMLVNLRNIFTRMDPTRQDIH
TLHGAVMAIAEGRKGPAKGGVLDGAQATKLRALLAERAAAGHGDDAGSMRGLARMLRRNPTDAERLLWEHLRKDRRFAGQ
FKRQTPVGRHIPDFVSFPHRIAIELVNPDESDAIATDRAARKDWLEARDYRVVLIEAAMVERDIAEVLGRLEAVVKG

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=248, Percent_Identity=31.4516129032258, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790865, Length=181, Percent_Identity=33.1491712707182, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 43446; Mature: 43315

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSGTDKSKPAADLAGPVVILVEPQLGENIGMCARAMGNFGLKRLRLVKPRDGWPNVHA
CCCCCCCCCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCC
RRSASGADHILDAVELFETVGEAVADCTLLFATTARAHDQAKPVRGPEAAAQEMITAAVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GGTAGILFGRERYGLENHEVALANRIVTFPVNPAFASLNLAQAVLLMGYEWFKHATGGAL
CCCEEEEECHHHCCCCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYAMPERSEPASQHQMTAFFDNLVAELDRVEFLRPPEKRDTMLVNLRNIFTRMDPTRQDI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHCCCCHHHH
HTLHGAVMAIAEGRKGPAKGGVLDGAQATKLRALLAERAAAGHGDDAGSMRGLARMLRRN
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PTDAERLLWEHLRKDRRFAGQFKRQTPVGRHIPDFVSFPHRIAIELVNPDESDAIATDRA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEEECCCCCCHHHHHHH
ARKDWLEARDYRVVLIEAAMVERDIAEVLGRLEAVVKG
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGSGTDKSKPAADLAGPVVILVEPQLGENIGMCARAMGNFGLKRLRLVKPRDGWPNVHA
CCCCCCCCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCC
RRSASGADHILDAVELFETVGEAVADCTLLFATTARAHDQAKPVRGPEAAAQEMITAAVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GGTAGILFGRERYGLENHEVALANRIVTFPVNPAFASLNLAQAVLLMGYEWFKHATGGAL
CCCEEEEECHHHCCCCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYAMPERSEPASQHQMTAFFDNLVAELDRVEFLRPPEKRDTMLVNLRNIFTRMDPTRQDI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHCCCCHHHH
HTLHGAVMAIAEGRKGPAKGGVLDGAQATKLRALLAERAAAGHGDDAGSMRGLARMLRRN
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PTDAERLLWEHLRKDRRFAGQFKRQTPVGRHIPDFVSFPHRIAIELVNPDESDAIATDRA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEEECCCCCCHHHHHHH
ARKDWLEARDYRVVLIEAAMVERDIAEVLGRLEAVVKG
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA