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The map label for this gene is queC2 [H]

Identifier: 86750585

GI number: 86750585

Start: 3979332

End: 3980027

Strand: Direct

Name: queC2 [H]

Synonym: RPB_3474

Alternate gene names: 86750585

Gene position: 3979332-3980027 (Clockwise)

Preceding gene: 86750581

Following gene: 86750586

Centisome position: 74.64

GC content: 52.73

Gene sequence:

>696_bases
ATGGCCAACTTCCCTAGCATTGCTCTATCCTTGCAACCGCGAGTCGCACATCAGGTTCAAAAGATGGCACGGAAAAAAGT
CCTGGTACTGCACAGTGGTGGCATGGACTCGACCACGTGTTTGCTGCAAGCTAAAGCTGAAGGACATGACGTAGCGAGCC
TAGGCATTGACTACGGGCAACGGCTGCTTGTCGAGATGATGTTCGCCGAGGGACAGTGCGAAAAATACAGCATTCCGCGC
CACGTTATAAACGTGAACTGGCAGAAAGCGGAACGCCAGATACCGTTGAACCGTTCGGTCGAAGAAATGGCCCACAGTGT
TTCCCCAGCCTTCCTCCCTGGCCGAAACATCGTCTTCCTTGGCTTGGGCCATGCCCACGCCGCAGGGATTGGCGCCGACG
AGTTGCAAATCGGATTAAACTGTGTCGACTTCTCAGGATACCCTGACTGCACGACCCAATTTTACGACTCCTACTGCACG
ATGCTCAACATCGGAAATCCGGGCGGACCAAAACTAGTTGCCCCCTTACTCAAGATGAGCAAACCAGAAATTGCTCGACT
TGCCAGCACACTTGGACTCCAACGAAATGATACATGGAGCTGTTATCGCCCCCAAATACGCGAGGGTTCCATTGTGGCTT
GCGGCGAATGTGACGCCTGCAAGCTTCACGAATTTGCTTGGCAAGAATTGAAATAG

Upstream 100 bases:

>100_bases
ACCCAACTGACGAAATCGTCATGACACCGGGCATCCGTCTAGCACTTTGGCCACACAAACGGAAAGACCGCTGGATCTTG
GCCGCGTGACTGTTTTACCA

Downstream 100 bases:

>100_bases
CTTTTGTCAACCATAGGCCTCATCGCATGCTCCAAGACAAAGCGCACGACTGGTGCGCCCGCGGCGCTGCTATACAGCTC
CGCCTTATTCAAAAAGTCGC

Product: ExsB

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase 2; PreQ(0) synthase 2; Queuosine biosynthesis protein queC 2 [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MANFPSIALSLQPRVAHQVQKMARKKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQRLLVEMMFAEGQCEKYSIPR
HVINVNWQKAERQIPLNRSVEEMAHSVSPAFLPGRNIVFLGLGHAHAAGIGADELQIGLNCVDFSGYPDCTTQFYDSYCT
MLNIGNPGGPKLVAPLLKMSKPEIARLASTLGLQRNDTWSCYRPQIREGSIVACGECDACKLHEFAWQELK

Sequences:

>Translated_231_residues
MANFPSIALSLQPRVAHQVQKMARKKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQRLLVEMMFAEGQCEKYSIPR
HVINVNWQKAERQIPLNRSVEEMAHSVSPAFLPGRNIVFLGLGHAHAAGIGADELQIGLNCVDFSGYPDCTTQFYDSYCT
MLNIGNPGGPKLVAPLLKMSKPEIARLASTLGLQRNDTWSCYRPQIREGSIVACGECDACKLHEFAWQELK
>Mature_230_residues
ANFPSIALSLQPRVAHQVQKMARKKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQRLLVEMMFAEGQCEKYSIPRH
VINVNWQKAERQIPLNRSVEEMAHSVSPAFLPGRNIVFLGLGHAHAAGIGADELQIGLNCVDFSGYPDCTTQFYDSYCTM
LNIGNPGGPKLVAPLLKMSKPEIARLASTLGLQRNDTWSCYRPQIREGSIVACGECDACKLHEFAWQELK

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)) [H]

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family [H]

Homologues:

Organism=Escherichia coli, GI1786648, Length=208, Percent_Identity=34.1346153846154, Blast_Score=103, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018317
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06508 ExsB [H]

EC number: NA

Molecular weight: Translated: 25414; Mature: 25282

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFPSIALSLQPRVAHQVQKMARKKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQ
CCCCCCEEEEECHHHHHHHHHHHHCEEEEEECCCCCCEEEEEEECCCCCCEEHHHHHHHH
RLLVEMMFAEGQCEKYSIPRHVINVNWQKAERQIPLNRSVEEMAHSVSPAFLPGRNIVFL
HHHHHHHHHCCCCCCCCCCCEEEECCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEE
GLGHAHAAGIGADELQIGLNCVDFSGYPDCTTQFYDSYCTMLNIGNPGGPKLVAPLLKMS
ECCCHHHCCCCHHHEEECCEEEECCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHCC
KPEIARLASTLGLQRNDTWSCYRPQIREGSIVACGECDACKLHEFAWQELK
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ANFPSIALSLQPRVAHQVQKMARKKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQ
CCCCCEEEEECHHHHHHHHHHHHCEEEEEECCCCCCEEEEEEECCCCCCEEHHHHHHHH
RLLVEMMFAEGQCEKYSIPRHVINVNWQKAERQIPLNRSVEEMAHSVSPAFLPGRNIVFL
HHHHHHHHHCCCCCCCCCCCEEEECCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEE
GLGHAHAAGIGADELQIGLNCVDFSGYPDCTTQFYDSYCTMLNIGNPGGPKLVAPLLKMS
ECCCHHHCCCCHHHEEECCEEEECCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHCC
KPEIARLASTLGLQRNDTWSCYRPQIREGSIVACGECDACKLHEFAWQELK
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA