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The map label for this gene is gph [H]

Identifier: 86750498

GI number: 86750498

Start: 3871733

End: 3872410

Strand: Direct

Name: gph [H]

Synonym: RPB_3387

Alternate gene names: 86750498

Gene position: 3871733-3872410 (Clockwise)

Preceding gene: 86750490

Following gene: 86750499

Centisome position: 72.62

GC content: 67.99

Gene sequence:

>678_bases
ATGAGCACATCCCGCACCGTTGTCTTCGACCTGGACGGCACGCTGATCGACACCGCGCCGGACCTGATCAATGCCCTGAA
TTACATCCTCGTCCGCGAAGGCATGCCGGCGGTCCCGCTCGCAAAGGCCCGCAATATGATCGGGCAAGGCGCGCGGCGAT
TGCTGGAGCGCGGCCTCGAACTCGACGGCCGCGTCATCAGCCCGGACGACGTCAACCGCCTCGCGGTCGATTTCATCGAC
TATTACGCCGCCAATATCGCCGTCGAGTCGCGGCCGTTCGAGGGACTGGAACAGACGCTCGATGCGCTCGCCGGCCAGGG
CTACCAGTTCGCGGTGTGCACCAACAAGCTGGAATGGCTGTCGAAGCTGCTGCTCGACCAGCTCGGCCTGAGCTCCCGCT
TCGCCGCGATCTGCGGCGCCGACACCTTCGGCGTCGCCAAACCCGACCCGGCGATCCTGCGCGAGACCATTGCCAAGGCC
GGCGGCGCACTGGCCTCGGCGGTGATGGTCGGCGACGCCGGACCGGATGTCGGCGTCGCGCGGCGCGCCGGCATCCCGGT
GATCGGCGTCGAGTTCGGCTACACCGAGGTTCCGATCGCCGAGCTCAAGCCGGACCGGCTGGTCGGCCATATGCGCGACT
TGCCGAACGCCGTCACGCAGCTGCTGCCGCCGGCCTGA

Upstream 100 bases:

>100_bases
ATATCTAGCGACGTTTGCGGCATAACAACAGGGTGGATTCCGTCGCCAGCCCGGCATCGGACGGTTGCGAAAATTCACAA
GGACAGCTAGCGCTTCCCGG

Downstream 100 bases:

>100_bases
CCGCGGCGATATCCGGTTGATCCCCCTGCACAAATGACGTGTGGACGACCGTTAACCAATACTTAAGATTGCGCCGACCC
CGGTTGCCAGCGCAGCTTCG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSTSRTVVFDLDGTLIDTAPDLINALNYILVREGMPAVPLAKARNMIGQGARRLLERGLELDGRVISPDDVNRLAVDFID
YYAANIAVESRPFEGLEQTLDALAGQGYQFAVCTNKLEWLSKLLLDQLGLSSRFAAICGADTFGVAKPDPAILRETIAKA
GGALASAVMVGDAGPDVGVARRAGIPVIGVEFGYTEVPIAELKPDRLVGHMRDLPNAVTQLLPPA

Sequences:

>Translated_225_residues
MSTSRTVVFDLDGTLIDTAPDLINALNYILVREGMPAVPLAKARNMIGQGARRLLERGLELDGRVISPDDVNRLAVDFID
YYAANIAVESRPFEGLEQTLDALAGQGYQFAVCTNKLEWLSKLLLDQLGLSSRFAAICGADTFGVAKPDPAILRETIAKA
GGALASAVMVGDAGPDVGVARRAGIPVIGVEFGYTEVPIAELKPDRLVGHMRDLPNAVTQLLPPA
>Mature_224_residues
STSRTVVFDLDGTLIDTAPDLINALNYILVREGMPAVPLAKARNMIGQGARRLLERGLELDGRVISPDDVNRLAVDFIDY
YAANIAVESRPFEGLEQTLDALAGQGYQFAVCTNKLEWLSKLLLDQLGLSSRFAAICGADTFGVAKPDPAILRETIAKAG
GALASAVMVGDAGPDVGVARRAGIPVIGVEFGYTEVPIAELKPDRLVGHMRDLPNAVTQLLPPA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=228, Percent_Identity=30.2631578947368, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23956; Mature: 23825

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSRTVVFDLDGTLIDTAPDLINALNYILVREGMPAVPLAKARNMIGQGARRLLERGLE
CCCCCEEEEECCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LDGRVISPDDVNRLAVDFIDYYAANIAVESRPFEGLEQTLDALAGQGYQFAVCTNKLEWL
CCCEEECCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
SKLLLDQLGLSSRFAAICGADTFGVAKPDPAILRETIAKAGGALASAVMVGDAGPDVGVA
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHEECCCCCCCCHH
RRAGIPVIGVEFGYTEVPIAELKPDRLVGHMRDLPNAVTQLLPPA
HHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STSRTVVFDLDGTLIDTAPDLINALNYILVREGMPAVPLAKARNMIGQGARRLLERGLE
CCCCEEEEECCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LDGRVISPDDVNRLAVDFIDYYAANIAVESRPFEGLEQTLDALAGQGYQFAVCTNKLEWL
CCCEEECCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
SKLLLDQLGLSSRFAAICGADTFGVAKPDPAILRETIAKAGGALASAVMVGDAGPDVGVA
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHEECCCCCCCCHH
RRAGIPVIGVEFGYTEVPIAELKPDRLVGHMRDLPNAVTQLLPPA
HHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]