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The map label for this gene is opuCB [H]

Identifier: 86750448

GI number: 86750448

Start: 3817274

End: 3818833

Strand: Direct

Name: opuCB [H]

Synonym: RPB_3337

Alternate gene names: 86750448

Gene position: 3817274-3818833 (Clockwise)

Preceding gene: 86750447

Following gene: 86750454

Centisome position: 71.6

GC content: 70.38

Gene sequence:

>1560_bases
ATGAGCCTGTTCGGCGATCCGCGCTGGAACGAGGCGCTGGCCAATCTGCCGGACACGCTCGGCGCGCATGTCCGCGTCAG
CCTCGCCGCGCTGGCGCTCGGCCTCGTCGTCAGCCTGCCGCTGGCGATTGTTGCGCGACGCCGCCCGATCCTGCGCGGCG
CCCTGCTCGGCTTCGCCGGCATCGTCCAGACCATCCCCGGCCTCGCTCTACTGGCGCTGTTCTATCCGCTGCTGCTGGCG
CTGGCGGCGTTGAGTCTCCGCGCCTTCGGCGTCGGCTTCTCGGCGTTCGGCTTCCTGCCCGCGGTGCTGGCGCTGGCGCT
GTATGCGATGCTGCCGGTGCTGCGCAACACCATCACCGGGCTCGACGGCGTCGATCCGGCGCTCGTCGAGGCGGCGCAGG
GCGTCGGCATGACGCCGCGGCAGGTGTTGACGATGGTCGAGGTACCGCTGGCGCTGCCGGTGATCATGGCCGGCATTCGC
ACAGCCGCCGTATGGGTGATCGGCACCGCGACGCTGTCCACGCCCATTGGCCAGACCAGCCTCGGCAACTACATCTTCGC
CGGGCTGCAGACGCAGAACTGGGTGCTGGTGCTGTTCGGCTGCGCCGCCGCCGCAGTGCTGGCGCTCGCGGTCGATCAAC
TGCTGGCGCTGATCGAGAGCGGCCTGCGGCTGCGCAGCCGCGTCCGCACCGCGCTCGGCGGCCTTGGCCTCGCCGCGCTG
CTGATCGCGACACTGGCGCCGTCGCTGGCCGGCACGCGATCGACCTACTTCGTCGGCGCCAAGACCTTCACCGAGCAATA
TGTGCTCGCGGCCCTTCTCCAGCAGCGGCTCGACGCCGCCGGGCTGTCGGCCACGACGCGCGCCGGCCTCGGCTCCAGCG
TGATCTACGACGCGCTGAGGAGCGGCGACATCGACGCCTATGTCGACTACTCCGGCACGCTGTGGGCCAATCAGTTCAAG
CACCCCGGCATCGCCGCGCGCGACCAGGTGCTGGCCGATCTGAAGCAGGATCTCGCCAAGGACGGCGTCACCCTGCTCGG
CGACCTCGGCTTCGCCAACGCCTATGCGCTGGCGATGCCGCGCGCCAGGGCCGAGGCGCTCGGTATCCGCTCGATCGCCG
ATCTCGCCGCGCGCGCGCCGGCGATGACGATCGCCGGCGACTACGAGTTCTTCTCCCGCCCGGAATGGGCCAATCTGCGC
CGCACCTATGGGCTCACTTTCAAGGCCGAACGGCAGATGCAGCCCGACTTCATGTACGCGGCCGTCGCCACCGGCGAGGT
CGATCTGATCGCCGGCTATACCTCGGACGGGCTGATCGCCAAATACGATCTCGTCGTGCTGGACGATCCCAAGCAGGCGA
TCCCGCCTTACGACGCGGTGCTGCTGATCTCACCAAAGCGCGCCGGCGACGCGAAGCTACGCGATGCGCTGACGCCGCTG
GTCGGCCGCATCGATATCGGCGCGATGCGCGCCGCCAATCTGCGCGCCGCCTCGGGCGGCGGCGACGGCACGCCCGACGC
GGTGGCGCGGGCGCTGTGGGACGGCATCAAGGCGCGCTGA

Upstream 100 bases:

>100_bases
AGCTGGCGCACAGCACCGAGCCCTATGTCACCGAACTCCTGCAGACGCCGCGCAGGCAGGCTCTCAGGCTCTCTGCGCTG
CTGCCACAGGACGGCGCGGT

Downstream 100 bases:

>100_bases
TCGCCGCGCGCTTACTTCGCGGGCCGCAGCCCGAGCCGCTTTGCCACCACCTTGTCGATCCACCGCGGCGGCAGCTTGGT
CGACAGCCAGAACTGCAGCG

Product: substrate-binding region of ABC-type glycine betaine transport system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSLFGDPRWNEALANLPDTLGAHVRVSLAALALGLVVSLPLAIVARRRPILRGALLGFAGIVQTIPGLALLALFYPLLLA
LAALSLRAFGVGFSAFGFLPAVLALALYAMLPVLRNTITGLDGVDPALVEAAQGVGMTPRQVLTMVEVPLALPVIMAGIR
TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWVLVLFGCAAAAVLALAVDQLLALIESGLRLRSRVRTALGGLGLAAL
LIATLAPSLAGTRSTYFVGAKTFTEQYVLAALLQQRLDAAGLSATTRAGLGSSVIYDALRSGDIDAYVDYSGTLWANQFK
HPGIAARDQVLADLKQDLAKDGVTLLGDLGFANAYALAMPRARAEALGIRSIADLAARAPAMTIAGDYEFFSRPEWANLR
RTYGLTFKAERQMQPDFMYAAVATGEVDLIAGYTSDGLIAKYDLVVLDDPKQAIPPYDAVLLISPKRAGDAKLRDALTPL
VGRIDIGAMRAANLRAASGGGDGTPDAVARALWDGIKAR

Sequences:

>Translated_519_residues
MSLFGDPRWNEALANLPDTLGAHVRVSLAALALGLVVSLPLAIVARRRPILRGALLGFAGIVQTIPGLALLALFYPLLLA
LAALSLRAFGVGFSAFGFLPAVLALALYAMLPVLRNTITGLDGVDPALVEAAQGVGMTPRQVLTMVEVPLALPVIMAGIR
TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWVLVLFGCAAAAVLALAVDQLLALIESGLRLRSRVRTALGGLGLAAL
LIATLAPSLAGTRSTYFVGAKTFTEQYVLAALLQQRLDAAGLSATTRAGLGSSVIYDALRSGDIDAYVDYSGTLWANQFK
HPGIAARDQVLADLKQDLAKDGVTLLGDLGFANAYALAMPRARAEALGIRSIADLAARAPAMTIAGDYEFFSRPEWANLR
RTYGLTFKAERQMQPDFMYAAVATGEVDLIAGYTSDGLIAKYDLVVLDDPKQAIPPYDAVLLISPKRAGDAKLRDALTPL
VGRIDIGAMRAANLRAASGGGDGTPDAVARALWDGIKAR
>Mature_518_residues
SLFGDPRWNEALANLPDTLGAHVRVSLAALALGLVVSLPLAIVARRRPILRGALLGFAGIVQTIPGLALLALFYPLLLAL
AALSLRAFGVGFSAFGFLPAVLALALYAMLPVLRNTITGLDGVDPALVEAAQGVGMTPRQVLTMVEVPLALPVIMAGIRT
AAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWVLVLFGCAAAAVLALAVDQLLALIESGLRLRSRVRTALGGLGLAALL
IATLAPSLAGTRSTYFVGAKTFTEQYVLAALLQQRLDAAGLSATTRAGLGSSVIYDALRSGDIDAYVDYSGTLWANQFKH
PGIAARDQVLADLKQDLAKDGVTLLGDLGFANAYALAMPRARAEALGIRSIADLAARAPAMTIAGDYEFFSRPEWANLRR
TYGLTFKAERQMQPDFMYAAVATGEVDLIAGYTSDGLIAKYDLVVLDDPKQAIPPYDAVLLISPKRAGDAKLRDALTPLV
GRIDIGAMRAANLRAASGGGDGTPDAVARALWDGIKAR

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1732

COG function: function code M; Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788451, Length=208, Percent_Identity=32.2115384615385, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788449, Length=183, Percent_Identity=34.9726775956284, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1789033, Length=195, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 54430; Mature: 54299

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFGDPRWNEALANLPDTLGAHVRVSLAALALGLVVSLPLAIVARRRPILRGALLGFAG
CCCCCCCCHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IVQTIPGLALLALFYPLLLALAALSLRAFGVGFSAFGFLPAVLALALYAMLPVLRNTITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDGVDPALVEAAQGVGMTPRQVLTMVEVPLALPVIMAGIRTAAVWVIGTATLSTPIGQTS
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCC
LGNYIFAGLQTQNWVLVLFGCAAAAVLALAVDQLLALIESGLRLRSRVRTALGGLGLAAL
CCCCEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIATLAPSLAGTRSTYFVGAKTFTEQYVLAALLQQRLDAAGLSATTRAGLGSSVIYDALR
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHH
SGDIDAYVDYSGTLWANQFKHPGIAARDQVLADLKQDLAKDGVTLLGDLGFANAYALAMP
CCCCCEEEECCCCEEHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCC
RARAEALGIRSIADLAARAPAMTIAGDYEFFSRPEWANLRRTYGLTFKAERQMQPDFMYA
HHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHHHHHCCEEEECCCCCCCCEEE
AVATGEVDLIAGYTSDGLIAKYDLVVLDDPKQAIPPYDAVLLISPKRAGDAKLRDALTPL
EEECCCEEEEEECCCCCCEEEEEEEEECCCHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
VGRIDIGAMRAANLRAASGGGDGTPDAVARALWDGIKAR
HHCCCCCHHHHCCCEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLFGDPRWNEALANLPDTLGAHVRVSLAALALGLVVSLPLAIVARRRPILRGALLGFAG
CCCCCCCHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IVQTIPGLALLALFYPLLLALAALSLRAFGVGFSAFGFLPAVLALALYAMLPVLRNTITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDGVDPALVEAAQGVGMTPRQVLTMVEVPLALPVIMAGIRTAAVWVIGTATLSTPIGQTS
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCC
LGNYIFAGLQTQNWVLVLFGCAAAAVLALAVDQLLALIESGLRLRSRVRTALGGLGLAAL
CCCCEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIATLAPSLAGTRSTYFVGAKTFTEQYVLAALLQQRLDAAGLSATTRAGLGSSVIYDALR
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHH
SGDIDAYVDYSGTLWANQFKHPGIAARDQVLADLKQDLAKDGVTLLGDLGFANAYALAMP
CCCCCEEEECCCCEEHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCC
RARAEALGIRSIADLAARAPAMTIAGDYEFFSRPEWANLRRTYGLTFKAERQMQPDFMYA
HHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHHHHHCCEEEECCCCCCCCEEE
AVATGEVDLIAGYTSDGLIAKYDLVVLDDPKQAIPPYDAVLLISPKRAGDAKLRDALTPL
EEECCCEEEEEECCCCCCEEEEEEEEECCCHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
VGRIDIGAMRAANLRAASGGGDGTPDAVARALWDGIKAR
HHCCCCCHHHHCCCEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]