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The map label for this gene is bioB

Identifier: 86750442

GI number: 86750442

Start: 3811502

End: 3812563

Strand: Direct

Name: bioB

Synonym: RPB_3331

Alternate gene names: 86750442

Gene position: 3811502-3812563 (Clockwise)

Preceding gene: 86750433

Following gene: 86750447

Centisome position: 71.49

GC content: 66.01

Gene sequence:

>1062_bases
TTGAACTCGATTGATCTGGCCTCACTGGCCGCCGCCACCCCCACCCTTCGCCACGACTGGACACGCGAACAGGCCGCCGC
GATTTACAACCTGCCGTTCGCCGACCTGATCTTCCGGGCGCAGACCATCCACCGCCAGAGCTTCGACGCCAACGAGGTGC
AGTGCAATCAGCTGCTCAACGTCAAGACCGGCGGCTGCGCCGAGGATTGCGGCTATTGCAGCCAGTCAGCGCATCACGAC
ACCGCCCTGCCCGCCTCCAAGCTGATGGACCCCAAGAAGGTCATCGAAGGCGCCAAGGCGGCTCGCGACGCAGGTGCCAC
CCGCTATTGCATGGGCGCGGCGTGGCGATCGCCGAAGGATCGCGACATGGCGCCGGTGATCGAGATGGTGAAGGGCGTCA
AGGCGCTCGGCATGGAAGCCTGCATGACGCTCGGCATGCTGACGGACGATCAGGCGCAGCAACTCGCCGACGCCGGCCTC
GATTACTACAACCACAACATCGACACCTCCGAGGAGTTCTACTCCTCCGTGGTCAAGTCCCGCAGCTTCGGCGACCGGCT
CGACACCCTCGCCACGGTGCAGGACGCCGGCATCAAGGTGTGCTGCGGCGGCATTCTCGGCCTTGGTGAGAAGCCGACCG
ACCGCGTCGAGATGCTGCGCACGCTCGCCAACCTGCCGCAGCATCCGGAGAGCGTGCCGATCAACATGCTGATCCCGATC
GAGGGCACGCCGATCGCCAAGACCGCCAAGCCGGTCGATCCGTTCGAGTTCGTCCGCACCATCGCGCTGGCGCGGATCAT
GATGCCGAAGTCGGACGTGCGGCTGGCCGCCGGCCGCACCGCGATGAGCGACGAGATGCAGTCGCTGTGTTTCCTCGCCG
GCGCCAATTCGATCTTCATCGGCGACACCCTGCTGACCACGCCGAACCCCGGCGACAGCAAGGACCGCATGCTGTTCGCC
CGGCTCGGCATCACCCCGCGCGAGGGTGCCCCGGTCGAGGCGCACAGCCACGACCACGATCACGATCACCACGACCATCA
TCACGGCCACAGCCACAGCTGA

Upstream 100 bases:

>100_bases
TACCTTTGGCCTAGCCCTCTTCGCGCGGTAAGCGGTTCCTTCCCGGATTGAAACGCCGTAAGGCTCTTGAGCCGGGAGAC
GACTGCATTGGAGTACGACG

Downstream 100 bases:

>100_bases
GGCCCGGACCCACAAAGGTCGTCATGGCCGGGCTTCGTCCCGGCCATCCACGGACGCGAGCACTGAGCCTATGGCAAGAC
GTGGATGGCCGGGACAAGCC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNSIDLASLAAATPTLRHDWTREQAAAIYNLPFADLIFRAQTIHRQSFDANEVQCNQLLNVKTGGCAEDCGYCSQSAHHD
TALPASKLMDPKKVIEGAKAARDAGATRYCMGAAWRSPKDRDMAPVIEMVKGVKALGMEACMTLGMLTDDQAQQLADAGL
DYYNHNIDTSEEFYSSVVKSRSFGDRLDTLATVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
EGTPIAKTAKPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQSLCFLAGANSIFIGDTLLTTPNPGDSKDRMLFA
RLGITPREGAPVEAHSHDHDHDHHDHHHGHSHS

Sequences:

>Translated_353_residues
MNSIDLASLAAATPTLRHDWTREQAAAIYNLPFADLIFRAQTIHRQSFDANEVQCNQLLNVKTGGCAEDCGYCSQSAHHD
TALPASKLMDPKKVIEGAKAARDAGATRYCMGAAWRSPKDRDMAPVIEMVKGVKALGMEACMTLGMLTDDQAQQLADAGL
DYYNHNIDTSEEFYSSVVKSRSFGDRLDTLATVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
EGTPIAKTAKPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQSLCFLAGANSIFIGDTLLTTPNPGDSKDRMLFA
RLGITPREGAPVEAHSHDHDHDHHDHHHGHSHS
>Mature_353_residues
MNSIDLASLAAATPTLRHDWTREQAAAIYNLPFADLIFRAQTIHRQSFDANEVQCNQLLNVKTGGCAEDCGYCSQSAHHD
TALPASKLMDPKKVIEGAKAARDAGATRYCMGAAWRSPKDRDMAPVIEMVKGVKALGMEACMTLGMLTDDQAQQLADAGL
DYYNHNIDTSEEFYSSVVKSRSFGDRLDTLATVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
EGTPIAKTAKPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQSLCFLAGANSIFIGDTLLTTPNPGDSKDRMLFA
RLGITPREGAPVEAHSHDHDHDHHDHHHGHSHS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=314, Percent_Identity=56.687898089172, Blast_Score=391, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=48.58934169279, Blast_Score=322, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_RHOP2 (Q2IUT3)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486938.1
- STRING:   Q2IUT3
- GeneID:   3911133
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3331
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2306521
- BioCyc:   RPAL316058:RPB_3331-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 38426; Mature: 38426

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIDLASLAAATPTLRHDWTREQAAAIYNLPFADLIFRAQTIHRQSFDANEVQCNQLLN
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHC
VKTGGCAEDCGYCSQSAHHDTALPASKLMDPKKVIEGAKAARDAGATRYCMGAAWRSPKD
CCCCCCHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
RDMAPVIEMVKGVKALGMEACMTLGMLTDDQAQQLADAGLDYYNHNIDTSEEFYSSVVKS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHH
RSFGDRLDTLATVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
CCHHHHHHHHHHHHHCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
EGTPIAKTAKPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQSLCFLAGANSIFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEE
GDTLLTTPNPGDSKDRMLFARLGITPREGAPVEAHSHDHDHDHHDHHHGHSHS
CCEEEECCCCCCCHHHEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSIDLASLAAATPTLRHDWTREQAAAIYNLPFADLIFRAQTIHRQSFDANEVQCNQLLN
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHC
VKTGGCAEDCGYCSQSAHHDTALPASKLMDPKKVIEGAKAARDAGATRYCMGAAWRSPKD
CCCCCCHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
RDMAPVIEMVKGVKALGMEACMTLGMLTDDQAQQLADAGLDYYNHNIDTSEEFYSSVVKS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHH
RSFGDRLDTLATVQDAGIKVCCGGILGLGEKPTDRVEMLRTLANLPQHPESVPINMLIPI
CCHHHHHHHHHHHHHCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEC
EGTPIAKTAKPVDPFEFVRTIALARIMMPKSDVRLAAGRTAMSDEMQSLCFLAGANSIFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEE
GDTLLTTPNPGDSKDRMLFARLGITPREGAPVEAHSHDHDHDHHDHHHGHSHS
CCEEEECCCCCCCHHHEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA