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The map label for this gene is lip2 [H]

Identifier: 86750145

GI number: 86750145

Start: 3451041

End: 3451985

Strand: Direct

Name: lip2 [H]

Synonym: RPB_3028

Alternate gene names: 86750145

Gene position: 3451041-3451985 (Clockwise)

Preceding gene: 86750144

Following gene: 86750146

Centisome position: 64.73

GC content: 67.94

Gene sequence:

>945_bases
ATGCCAGCAAAACTCGATCCCGATGCCGCCGCCGTCTACAAGGCGTTCCAGGACGCGGGCCGCCCCGCCTATGAAGATCT
CACGGCCGAGGAAGCGCGCGCGTACTACGACGCCGCCCGGCTCGTCAGCAATCCCGACCCGCGCGAACTCGCCTCGATCC
AGTCGATCGCGATCCCTGGACCCGCAGGCGACATCCCGGCCCGCGTCTACACGCCGAAGACACTGCGTCAGGACAACGGT
CTGGCGCCGGCGCTGGTGTTCTTTCACGGCGGCGGCTGGGTGATCGGCAATCTCGACACCCATGACGTCGTCTGCCGCGC
CATCGCCGACGAAGGCCAACTGATCGTCATCTCGGTCGACTACCGGCTCGCCCCCGAGCACAAATTCCCGGCCGCCGTCG
AGGACGCGATCGCGGCGACCCAATGGGTCGCCGACAATGCGCGCAAGCTCGGCATCGATCCCGAACGCATCAGCGTCGGC
GGCGACAGCGCCGGCGGCAATCTGTCGGCCGTGGTGACGATCCACGCCCGCGACCATGGCGGGCCGATGCTCGCCGGCCA
GGTGCTGATCTATCCGGCCACCGACTTCGCGATGGACCATCCGTCGCACAGCGAACCGGAGACCAGCGTGCTGCTGACGC
ATTCCGTGATCAGATGGTTTCGCGATCACTATCTCGGCGGCGCACCGGTCGAACAGGACTGGCGCGCCTCCCCGGCCCGC
GTCGAAACCCTGGCCGGGCTGCCGCCGGCCTTCGTCATCACCGCCGGCGCCGACCCGCTGCGCGACGAAGGCGACGAATA
CGCGCGTCGCCTCGCCGATGCCGGCGTGCCGGTGACGCATCGGACTTATCCCGGCCAATTCCACGGCTTCTTCACCATGG
GCAAGCTGCTGCCGAAGGCGAACGACGCGGTCGTCGAAATCGGCGATTGGCTGAAGGCGCTGTAG

Upstream 100 bases:

>100_bases
CGCCTCCGACGAGGCGAGCTACGTGTCGGGCGCGGCCTACACCGTCGACGGCGGCCGCACCGCGAGCTGATCGTTCTCTC
TCACCAGGTCCAGCAAAACC

Downstream 100 bases:

>100_bases
CCAGCGCGGATTGACCGGCGTCAACGTCGTCGCGCGCCGAACCGGCGAGGTTGGCCCCCCGCAATCAGAGGGCCGCCGAC
GCGCGGCGCAACGGCGTTCG

Product: esterase/lipase/thioesterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPAKLDPDAAAVYKAFQDAGRPAYEDLTAEEARAYYDAARLVSNPDPRELASIQSIAIPGPAGDIPARVYTPKTLRQDNG
LAPALVFFHGGGWVIGNLDTHDVVCRAIADEGQLIVISVDYRLAPEHKFPAAVEDAIAATQWVADNARKLGIDPERISVG
GDSAGGNLSAVVTIHARDHGGPMLAGQVLIYPATDFAMDHPSHSEPETSVLLTHSVIRWFRDHYLGGAPVEQDWRASPAR
VETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVTHRTYPGQFHGFFTMGKLLPKANDAVVEIGDWLKAL

Sequences:

>Translated_314_residues
MPAKLDPDAAAVYKAFQDAGRPAYEDLTAEEARAYYDAARLVSNPDPRELASIQSIAIPGPAGDIPARVYTPKTLRQDNG
LAPALVFFHGGGWVIGNLDTHDVVCRAIADEGQLIVISVDYRLAPEHKFPAAVEDAIAATQWVADNARKLGIDPERISVG
GDSAGGNLSAVVTIHARDHGGPMLAGQVLIYPATDFAMDHPSHSEPETSVLLTHSVIRWFRDHYLGGAPVEQDWRASPAR
VETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVTHRTYPGQFHGFFTMGKLLPKANDAVVEIGDWLKAL
>Mature_313_residues
PAKLDPDAAAVYKAFQDAGRPAYEDLTAEEARAYYDAARLVSNPDPRELASIQSIAIPGPAGDIPARVYTPKTLRQDNGL
APALVFFHGGGWVIGNLDTHDVVCRAIADEGQLIVISVDYRLAPEHKFPAAVEDAIAATQWVADNARKLGIDPERISVGG
DSAGGNLSAVVTIHARDHGGPMLAGQVLIYPATDFAMDHPSHSEPETSVLLTHSVIRWFRDHYLGGAPVEQDWRASPARV
ETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVTHRTYPGQFHGFFTMGKLLPKANDAVVEIGDWLKAL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=342, Percent_Identity=30.7017543859649, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI206597554, Length=287, Percent_Identity=32.0557491289199, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI61966717, Length=290, Percent_Identity=28.9655172413793, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI68051721, Length=277, Percent_Identity=28.5198555956679, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI157041239, Length=159, Percent_Identity=36.4779874213836, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI226423947, Length=285, Percent_Identity=27.719298245614, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI21328446, Length=110, Percent_Identity=40.9090909090909, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI157041237, Length=251, Percent_Identity=26.2948207171315, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI68508967, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI68508965, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI68508957, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1786682, Length=280, Percent_Identity=28.9285714285714, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17540028, Length=318, Percent_Identity=28.6163522012579, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17567059, Length=273, Percent_Identity=32.2344322344322, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996133, Length=134, Percent_Identity=38.8059701492537, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI72001146, Length=130, Percent_Identity=36.9230769230769, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115533410, Length=142, Percent_Identity=30.2816901408451, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=142, Percent_Identity=30.2816901408451, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656084, Length=114, Percent_Identity=36.8421052631579, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656076, Length=114, Percent_Identity=36.8421052631579, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130169, Length=114, Percent_Identity=36.8421052631579, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33724; Mature: 33593

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLDPDAAAVYKAFQDAGRPAYEDLTAEEARAYYDAARLVSNPDPRELASIQSIAIPG
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCC
PAGDIPARVYTPKTLRQDNGLAPALVFFHGGGWVIGNLDTHDVVCRAIADEGQLIVISVD
CCCCCCCEEECCHHHHCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHCCCCCEEEEEEC
YRLAPEHKFPAAVEDAIAATQWVADNARKLGIDPERISVGGDSAGGNLSAVVTIHARDHG
CEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCCCEEEEEEEEEECCC
GPMLAGQVLIYPATDFAMDHPSHSEPETSVLLTHSVIRWFRDHYLGGAPVEQDWRASPAR
CCEEECEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVTHRTYPGQFHGFFTMGKLLPKA
HHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCC
NDAVVEIGDWLKAL
CCCEEEHHHHHHCC
>Mature Secondary Structure 
PAKLDPDAAAVYKAFQDAGRPAYEDLTAEEARAYYDAARLVSNPDPRELASIQSIAIPG
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCC
PAGDIPARVYTPKTLRQDNGLAPALVFFHGGGWVIGNLDTHDVVCRAIADEGQLIVISVD
CCCCCCCEEECCHHHHCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHCCCCCEEEEEEC
YRLAPEHKFPAAVEDAIAATQWVADNARKLGIDPERISVGGDSAGGNLSAVVTIHARDHG
CEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCCCEEEEEEEEEECCC
GPMLAGQVLIYPATDFAMDHPSHSEPETSVLLTHSVIRWFRDHYLGGAPVEQDWRASPAR
CCEEECEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVTHRTYPGQFHGFFTMGKLLPKA
HHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCC
NDAVVEIGDWLKAL
CCCEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]