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The map label for this gene is pleD [H]

Identifier: 86750103

GI number: 86750103

Start: 3401005

End: 3402063

Strand: Direct

Name: pleD [H]

Synonym: RPB_2986

Alternate gene names: 86750103

Gene position: 3401005-3402063 (Clockwise)

Preceding gene: 86750102

Following gene: 86750113

Centisome position: 63.79

GC content: 65.63

Gene sequence:

>1059_bases
ATGCAGAATGACCACGAACGCAGCATGGCGTATGCCGAGCTGGCGCTGAACCAGATCCGGGCGCTGCGGCAGGGCGCGAT
GCCGCGCAACTACGAGATCTGGTACGTCTACGCCACCGGCTACAACGCCGAGCTCAACAAGGCGATCAACGAGGCGCTGG
CCCAGAACGGCCGGCTGTCGGACGCCGACCTCGAGCAGCTACACGATAGCTATTTGTCGCACGGCCGCACCCACGAGCGC
ATCGACCGGATCGGCGCCCGCGTCGTCAACGAGATCGACGACATGATGTCGCTGCTGTCCGATGCGCTCGGCACCACCGC
GACGTTCGGCGACAACCTGCAGGGCGCGAGCCAGAACCTGTCGATCGCCACCGACCACGAGCAGATCAAGGCGATCATCG
AGCGGCTGGTCGGGTCGACCCGCGAGATGCAGCAGGCCAACCACGCCCTCGAAGAGCGGCTGTCCTCGTCGCGGCAGGAA
ATCACCAATCTGCAGGCCAGCCTCGAGGCGATCAGCGCCGAGAGCCTGACCGATCCCTTGACCGGCCTCGGCAACCGCAA
GCATTTCGACCGCGTCGTCACCGAAGCGGTGCGCAGCGCCACGGTGAGCCGGCTGCCGCTGTCGCTGCTGATGATGGACA
TCGATCACTTCAAGTCGTTCAACGACAATTACGGCCACCTCACCGGCGATCAGGTGCTGCGGCTGGTCGGCATGACGTTG
AAGCAGAGCATCAAGACCAAGAACGTCCCGGCGCGCTACGGCGGCGAGGAATTCGCCGTGGTGCTGCCGGACACGCCGCT
GCGCCAGGCGCTGATGGTCGCCGACGACATCCGCCGCGTGGTGATGTCGAAGGAATTGAAGAAGAAGTCGACCGGCGAGA
TCCTCGGCCGGGTGACGCTGTCGGCCGGCGTCTCGACGCTGAAGCCGGACGACGACGCCGACGCGCTGATCGAACGCGCC
GACGCCTGCCTGTATTCGGCCAAGCGCAACGGCCGCAACCGGGTGATCTGCGAAACCGATCCTGAATTCCGCAGCGTCGC
GCCCGCCAAGGTCGCGTAG

Upstream 100 bases:

>100_bases
CCCGGGACATTTACCCGGCATTCACCCTCGCAACGTAGCCTTGCAACCGTTGATGCAGGGTTGGGGGCAGTCCCCCCACG
ACGGGGAGCATCTGGCCGCC

Downstream 100 bases:

>100_bases
CGCCGCAACTAAGCTCCGCGCTGCCGCACGCGCTTCGGCTTCGCGGCGCTGGCCGCCGCGCGCGGCTTGCTCTTCGGCTT
CCGCGTCTTCGTCAGCGCCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MQNDHERSMAYAELALNQIRALRQGAMPRNYEIWYVYATGYNAELNKAINEALAQNGRLSDADLEQLHDSYLSHGRTHER
IDRIGARVVNEIDDMMSLLSDALGTTATFGDNLQGASQNLSIATDHEQIKAIIERLVGSTREMQQANHALEERLSSSRQE
ITNLQASLEAISAESLTDPLTGLGNRKHFDRVVTEAVRSATVSRLPLSLLMMDIDHFKSFNDNYGHLTGDQVLRLVGMTL
KQSIKTKNVPARYGGEEFAVVLPDTPLRQALMVADDIRRVVMSKELKKKSTGEILGRVTLSAGVSTLKPDDDADALIERA
DACLYSAKRNGRNRVICETDPEFRSVAPAKVA

Sequences:

>Translated_352_residues
MQNDHERSMAYAELALNQIRALRQGAMPRNYEIWYVYATGYNAELNKAINEALAQNGRLSDADLEQLHDSYLSHGRTHER
IDRIGARVVNEIDDMMSLLSDALGTTATFGDNLQGASQNLSIATDHEQIKAIIERLVGSTREMQQANHALEERLSSSRQE
ITNLQASLEAISAESLTDPLTGLGNRKHFDRVVTEAVRSATVSRLPLSLLMMDIDHFKSFNDNYGHLTGDQVLRLVGMTL
KQSIKTKNVPARYGGEEFAVVLPDTPLRQALMVADDIRRVVMSKELKKKSTGEILGRVTLSAGVSTLKPDDDADALIERA
DACLYSAKRNGRNRVICETDPEFRSVAPAKVA
>Mature_352_residues
MQNDHERSMAYAELALNQIRALRQGAMPRNYEIWYVYATGYNAELNKAINEALAQNGRLSDADLEQLHDSYLSHGRTHER
IDRIGARVVNEIDDMMSLLSDALGTTATFGDNLQGASQNLSIATDHEQIKAIIERLVGSTREMQQANHALEERLSSSRQE
ITNLQASLEAISAESLTDPLTGLGNRKHFDRVVTEAVRSATVSRLPLSLLMMDIDHFKSFNDNYGHLTGDQVLRLVGMTL
KQSIKTKNVPARYGGEEFAVVLPDTPLRQALMVADDIRRVVMSKELKKKSTGEILGRVTLSAGVSTLKPDDDADALIERA
DACLYSAKRNGRNRVICETDPEFRSVAPAKVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=178, Percent_Identity=37.0786516853933, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081881, Length=203, Percent_Identity=34.9753694581281, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=38.7878787878788, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787802, Length=218, Percent_Identity=33.0275229357798, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=34.0659340659341, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787816, Length=188, Percent_Identity=35.1063829787234, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=31.2849162011173, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=191, Percent_Identity=31.9371727748691, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=30.7262569832402, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=28.0487804878049, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788956, Length=185, Percent_Identity=30.8108108108108, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787056, Length=172, Percent_Identity=31.3953488372093, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=31.547619047619, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=29.608938547486, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39023; Mature: 39023

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNDHERSMAYAELALNQIRALRQGAMPRNYEIWYVYATGYNAELNKAINEALAQNGRLS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCC
DADLEQLHDSYLSHGRTHERIDRIGARVVNEIDDMMSLLSDALGTTATFGDNLQGASQNL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
SIATDHEQIKAIIERLVGSTREMQQANHALEERLSSSRQEITNLQASLEAISAESLTDPL
CEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TGLGNRKHFDRVVTEAVRSATVSRLPLSLLMMDIDHFKSFNDNYGHLTGDQVLRLVGMTL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KQSIKTKNVPARYGGEEFAVVLPDTPLRQALMVADDIRRVVMSKELKKKSTGEILGRVTL
HHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGVSTLKPDDDADALIERADACLYSAKRNGRNRVICETDPEFRSVAPAKVA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCC
>Mature Secondary Structure
MQNDHERSMAYAELALNQIRALRQGAMPRNYEIWYVYATGYNAELNKAINEALAQNGRLS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCC
DADLEQLHDSYLSHGRTHERIDRIGARVVNEIDDMMSLLSDALGTTATFGDNLQGASQNL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
SIATDHEQIKAIIERLVGSTREMQQANHALEERLSSSRQEITNLQASLEAISAESLTDPL
CEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TGLGNRKHFDRVVTEAVRSATVSRLPLSLLMMDIDHFKSFNDNYGHLTGDQVLRLVGMTL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KQSIKTKNVPARYGGEEFAVVLPDTPLRQALMVADDIRRVVMSKELKKKSTGEILGRVTL
HHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGVSTLKPDDDADALIERADACLYSAKRNGRNRVICETDPEFRSVAPAKVA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA