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The map label for this gene is trmFO

Identifier: 86749845

GI number: 86749845

Start: 3111557

End: 3113005

Strand: Direct

Name: trmFO

Synonym: RPB_2728

Alternate gene names: 86749845

Gene position: 3111557-3113005 (Clockwise)

Preceding gene: 86749844

Following gene: 86749851

Centisome position: 58.36

GC content: 69.29

Gene sequence:

>1449_bases
ATGATCATTGCCGCGCCCCCTCCCCAACCCGCGTCCAACCAGCCTGTTCACGTCATCGGCGGCGGCCTCGCCGGCTCCGA
AGCCACCTGGCAATTGGCGCGCGCCGGCGTGCGCGTCGTGCTGCACGAGATGCGGCCGCATCGCTCGACAGAGGCGCACA
AGACCGACGGCCTCGCCGAGCTGGTCTGCTCCAATTCCTTCCGCTCCGATGACGCCGCCAACAACGCGGTCGGGCTGCTG
CACGCCGAGATGCGCCGGCTCGGCTCGCTGGTGATGCGCGCGGCCGACGCCAATCAGGTCCCGGCCGGCGGCGCGCTGGC
GGTCGATCGCGACGGCTTCTCCGCCGCCGTCACGAAGGCGCTGGCCGAGCATCCGCTGATCGAGATCCGCCGCGAGGAAA
TCGCCGGCCTGCCGCCGGAGGACTGGGGCAACGTCATCGTCGCCACCGGCCCGCTGACCTCGGCGGCGCTGGCCGAGGCG
GTCCGCGCGCTGACCGATGAATCCGCGCTGGCGTTCTTCGACGCCATCGCCCCGATCGTGCATCGCGACTCGATCGACAT
GTCGATCGCCTGGTTCCAGTCGCGCTACGACAAGGCCGGCCCCGGCGGCAGCGGCGCCGACTATCTCAACTGCCCGATGA
ATCGCGAGCAGTACGACGCTTTCGTCGACGCGCTGATCGCCGGCGACAAGGTCGATTTCAAGGACTGGGAGAAGGACACG
CCGTATTTCGACGGCTGCCTGCCGATCGAGGTGATGGCCGAGCGCGGCCGCGAGACGCTGCGCCACGGGCCGATGAAGCC
GGTCGGGCTCACCAATCCGCACAACCCGACGGTCAAGGCCTACGCCATCGTCCAGCTCCGCCAGGACAACAAGCTCGGCA
CGCTGTTCAACATGGTCGGCTTCCAGACCAAGCTGAACTACGCCGCGCAGCAACGCGTCTTCCGCACCATCCCGGGGCTG
GAAAATGCTGAATTTGCAAGGCTCGGCGGGCTGCATCGCAACACGTTTCTGAACTCGCCGAAGCTGCTCGATGCCGGCCT
GCGGCTGCGCGCCGCGCCGCGGCTGCGGTTCGCCGGCCAGATGACCGGCTGCGAAGGCTATGTCGAATCAGCCGGCATCG
GTCTCGTCGCCGGGCTCAGCGCCGCCGCCGACGCGCTCGGCCGATCCATGACGACGCCGCCGCCGACCACCGCGCTCGGC
GCCCTGCTCGGCCACATCACCGGCGGCCATATCGAGACCATCGACGCCGGCCCGCGCTCGTTTCAGCCGATGAACATCAA
TTTCGGCCTGTTCCCGCCGCTGGCGCAGGCGCCGACCCACGGCCCCGACGGCAAGAAGCTGCGCGGCCCGGAAAAGTCGG
TCGCCAAGAAACAGGCGCTCAGCGCCCGCGCGCTGGCGGATCTGGATGCGTGGATCGCTGCAAGCCTGAAGCTACCGGCG
GCGGCGTAG

Upstream 100 bases:

>100_bases
GGGCGTTGTCGTATCCGGGCCATGCTGATCGGCCGCCCTGACCTGGCCGCGACCGCCGAAACGGATTTGACCCGGGGCGG
AAACGCGCTAGCTGTTGCGC

Downstream 100 bases:

>100_bases
CGCCTCTGCCGATGCATCCGACATTTGTTGCGGCGTCAGCTGTTGTGCGCCCGCCACAACTACTCCGTCATCCTGAGGCG
CCGCCGCGCAGCGGCCGCCT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIIAAPPPQPASNQPVHVIGGGLAGSEATWQLARAGVRVVLHEMRPHRSTEAHKTDGLAELVCSNSFRSDDAANNAVGLL
HAEMRRLGSLVMRAADANQVPAGGALAVDRDGFSAAVTKALAEHPLIEIRREEIAGLPPEDWGNVIVATGPLTSAALAEA
VRALTDESALAFFDAIAPIVHRDSIDMSIAWFQSRYDKAGPGGSGADYLNCPMNREQYDAFVDALIAGDKVDFKDWEKDT
PYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLFNMVGFQTKLNYAAQQRVFRTIPGL
ENAEFARLGGLHRNTFLNSPKLLDAGLRLRAAPRLRFAGQMTGCEGYVESAGIGLVAGLSAAADALGRSMTTPPPTTALG
ALLGHITGGHIETIDAGPRSFQPMNINFGLFPPLAQAPTHGPDGKKLRGPEKSVAKKQALSARALADLDAWIAASLKLPA
AA

Sequences:

>Translated_482_residues
MIIAAPPPQPASNQPVHVIGGGLAGSEATWQLARAGVRVVLHEMRPHRSTEAHKTDGLAELVCSNSFRSDDAANNAVGLL
HAEMRRLGSLVMRAADANQVPAGGALAVDRDGFSAAVTKALAEHPLIEIRREEIAGLPPEDWGNVIVATGPLTSAALAEA
VRALTDESALAFFDAIAPIVHRDSIDMSIAWFQSRYDKAGPGGSGADYLNCPMNREQYDAFVDALIAGDKVDFKDWEKDT
PYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLFNMVGFQTKLNYAAQQRVFRTIPGL
ENAEFARLGGLHRNTFLNSPKLLDAGLRLRAAPRLRFAGQMTGCEGYVESAGIGLVAGLSAAADALGRSMTTPPPTTALG
ALLGHITGGHIETIDAGPRSFQPMNINFGLFPPLAQAPTHGPDGKKLRGPEKSVAKKQALSARALADLDAWIAASLKLPA
AA
>Mature_482_residues
MIIAAPPPQPASNQPVHVIGGGLAGSEATWQLARAGVRVVLHEMRPHRSTEAHKTDGLAELVCSNSFRSDDAANNAVGLL
HAEMRRLGSLVMRAADANQVPAGGALAVDRDGFSAAVTKALAEHPLIEIRREEIAGLPPEDWGNVIVATGPLTSAALAEA
VRALTDESALAFFDAIAPIVHRDSIDMSIAWFQSRYDKAGPGGSGADYLNCPMNREQYDAFVDALIAGDKVDFKDWEKDT
PYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLFNMVGFQTKLNYAAQQRVFRTIPGL
ENAEFARLGGLHRNTFLNSPKLLDAGLRLRAAPRLRFAGQMTGCEGYVESAGIGLVAGLSAAADALGRSMTTPPPTTALG
ALLGHITGGHIETIDAGPRSFQPMNINFGLFPPLAQAPTHGPDGKKLRGPEKSVAKKQALSARALADLDAWIAASLKLPA
AA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_RHOP2 (Q2IWI0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486341.1
- STRING:   Q2IWI0
- GeneID:   3910521
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2728
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   RPAL316058:RPB_2728-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 51235; Mature: 51235

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIAAPPPQPASNQPVHVIGGGLAGSEATWQLARAGVRVVLHEMRPHRSTEAHKTDGLAE
CEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHH
LVCSNSFRSDDAANNAVGLLHAEMRRLGSLVMRAADANQVPAGGALAVDRDGFSAAVTKA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
LAEHPLIEIRREEIAGLPPEDWGNVIVATGPLTSAALAEAVRALTDESALAFFDAIAPIV
HHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
HRDSIDMSIAWFQSRYDKAGPGGSGADYLNCPMNREQYDAFVDALIAGDKVDFKDWEKDT
HCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
PYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLFNMVG
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHH
FQTKLNYAAQQRVFRTIPGLENAEFARLGGLHRNTFLNSPKLLDAGLRLRAAPRLRFAGQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHCCCCHHHHCCCEEECCCCCCCCCC
MTGCEGYVESAGIGLVAGLSAAADALGRSMTTPPPTTALGALLGHITGGHIETIDAGPRS
CCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCC
FQPMNINFGLFPPLAQAPTHGPDGKKLRGPEKSVAKKQALSARALADLDAWIAASLKLPA
CCCEECCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AA
CC
>Mature Secondary Structure
MIIAAPPPQPASNQPVHVIGGGLAGSEATWQLARAGVRVVLHEMRPHRSTEAHKTDGLAE
CEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHH
LVCSNSFRSDDAANNAVGLLHAEMRRLGSLVMRAADANQVPAGGALAVDRDGFSAAVTKA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
LAEHPLIEIRREEIAGLPPEDWGNVIVATGPLTSAALAEAVRALTDESALAFFDAIAPIV
HHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
HRDSIDMSIAWFQSRYDKAGPGGSGADYLNCPMNREQYDAFVDALIAGDKVDFKDWEKDT
HCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
PYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLFNMVG
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHH
FQTKLNYAAQQRVFRTIPGLENAEFARLGGLHRNTFLNSPKLLDAGLRLRAAPRLRFAGQ
HHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHCCCCHHHHCCCEEECCCCCCCCCC
MTGCEGYVESAGIGLVAGLSAAADALGRSMTTPPPTTALGALLGHITGGHIETIDAGPRS
CCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCC
FQPMNINFGLFPPLAQAPTHGPDGKKLRGPEKSVAKKQALSARALADLDAWIAASLKLPA
CCCEECCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA