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The map label for this gene is pncB [H]

Identifier: 86749710

GI number: 86749710

Start: 2973629

End: 2974945

Strand: Direct

Name: pncB [H]

Synonym: RPB_2592

Alternate gene names: 86749710

Gene position: 2973629-2974945 (Clockwise)

Preceding gene: 86749709

Following gene: 86749711

Centisome position: 55.77

GC content: 62.19

Gene sequence:

>1317_bases
ATGAGTTTTACCGATATCGCCACCCGGGTTCACAACCACAACTACCGGATGGACCCCGTCGTCCGCACGCTGCTCGACAC
CGATTTCTACAAGCTGCTGATGCTGCAGCTGATCTGGATGAAGCATCGGGATACGCCGGTGACGTTCGGGGTGATCAACC
GATCGAAATCGATCCGGATCGCAGACGAGATCGATATCGGCGAATTGCGGGCGCAACTGGACCACGCGAGAACGCTTCGG
CTGTCCAAGCGGGAAATGATCTGGCTGGCCGGCAATACGTTCTACGGCAAGAAGCAGATCTTCCGACCCGAATTTTTGTC
CTGGCTCGAGACCTTCGCGCTGCCGGAATACGAGGTCGACGTTCGTAACGGCCAGTACGAACTTCGCTTCGCCGGGCCAT
GGTCGCTGACGACCATGTGGGAAATTCCGGCGCTTTCGATCATCAACGAGCTGCGCGCGCGCAAAGCGATGCGCAATATG
GGGCGCTTCGAGCTCGACGTCCTTTATGCGCGCGCCAAGGCGAAGCTGTGGGGCAAAGTCGAGCGTCTGCAGGTGCTCAA
GGCGGCCGGCCGGCTGAAGGTCGGTGATTTCGGCACGCGCCGTCGGCACGGCTATCTGTGGCAGAGATGGTGCTGCGAAG
CCCTGCAGGAAGGCATCGGCGACGCCTTCATCGGCACCAGCAACGTCCACGTCGCCATGGAAATCGGCTCGGAAGCCATC
GGCACGAACGCCCATGAACTTTCAATGGTCTATGCGGCGCTGGCCGGCGATGACGATCAGATGGTGGCCGCGTCGCGCTA
CGCGGTGCTCGACGATTGGCGCCACCTGTATGACGGCAACCTGCTGGTGATCCTGCCGGACACCTATGGGACGACCTCGT
TCCTGCGCAATGCGCCCGACTGGGTGGCGGATTGGACCGGCGCGCGCCCGGACAGCAAGCCGCCGATCGCGGGGGGCGAC
GAGTTCATCGACTGGTGGAAAGCGAATGGTCGGGACCCGAAGGAGAAGCTCCTCGTCCTGTCCGACGGCATGGATATCGA
CTCGATCGAGCAGTCATTCCGGCATTTCAAGGATCGCGTCCGGGTCAGCTTCGGCTGGGGCACCAACCTGACCAACGATT
TCCGCGGCTGCGCGCCGAACGGCGGCAATGCTCTCGATCCGATCTCGCTGGTGTGCAAGGTCGTCGAGGCGGCCGGTCGC
CCGGCCGTGAAACTGTCCGACAACCCGACGAAGGCGACGGGTCCCGACGCGGATGTCGGGCGTTATCTTCAAATCTTCGG
GACGGAAGGCATGACGGATCACGCCGTCGACGTGTAG

Upstream 100 bases:

>100_bases
TCGGGCGGCTGTCGAAGCTGTTGGGATTTCCGCTGTAGCGTCCGCTTGGGCGCGCCTCGCGATCGTCGCCTGCGCAAGCC
CCACCCCGAGGGTGTCCGAG

Downstream 100 bases:

>100_bases
TCGATTCGCAGCGAGGAATTCGCCGGCGCTCCGGGCGCCGCGTCGCGCGGCTTGCGGTACGATTCGTGTTCTGCATGCGA
GCCTGTGTCGACTGCGCCCG

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSFTDIATRVHNHNYRMDPVVRTLLDTDFYKLLMLQLIWMKHRDTPVTFGVINRSKSIRIADEIDIGELRAQLDHARTLR
LSKREMIWLAGNTFYGKKQIFRPEFLSWLETFALPEYEVDVRNGQYELRFAGPWSLTTMWEIPALSIINELRARKAMRNM
GRFELDVLYARAKAKLWGKVERLQVLKAAGRLKVGDFGTRRRHGYLWQRWCCEALQEGIGDAFIGTSNVHVAMEIGSEAI
GTNAHELSMVYAALAGDDDQMVAASRYAVLDDWRHLYDGNLLVILPDTYGTTSFLRNAPDWVADWTGARPDSKPPIAGGD
EFIDWWKANGRDPKEKLLVLSDGMDIDSIEQSFRHFKDRVRVSFGWGTNLTNDFRGCAPNGGNALDPISLVCKVVEAAGR
PAVKLSDNPTKATGPDADVGRYLQIFGTEGMTDHAVDV

Sequences:

>Translated_438_residues
MSFTDIATRVHNHNYRMDPVVRTLLDTDFYKLLMLQLIWMKHRDTPVTFGVINRSKSIRIADEIDIGELRAQLDHARTLR
LSKREMIWLAGNTFYGKKQIFRPEFLSWLETFALPEYEVDVRNGQYELRFAGPWSLTTMWEIPALSIINELRARKAMRNM
GRFELDVLYARAKAKLWGKVERLQVLKAAGRLKVGDFGTRRRHGYLWQRWCCEALQEGIGDAFIGTSNVHVAMEIGSEAI
GTNAHELSMVYAALAGDDDQMVAASRYAVLDDWRHLYDGNLLVILPDTYGTTSFLRNAPDWVADWTGARPDSKPPIAGGD
EFIDWWKANGRDPKEKLLVLSDGMDIDSIEQSFRHFKDRVRVSFGWGTNLTNDFRGCAPNGGNALDPISLVCKVVEAAGR
PAVKLSDNPTKATGPDADVGRYLQIFGTEGMTDHAVDV
>Mature_437_residues
SFTDIATRVHNHNYRMDPVVRTLLDTDFYKLLMLQLIWMKHRDTPVTFGVINRSKSIRIADEIDIGELRAQLDHARTLRL
SKREMIWLAGNTFYGKKQIFRPEFLSWLETFALPEYEVDVRNGQYELRFAGPWSLTTMWEIPALSIINELRARKAMRNMG
RFELDVLYARAKAKLWGKVERLQVLKAAGRLKVGDFGTRRRHGYLWQRWCCEALQEGIGDAFIGTSNVHVAMEIGSEAIG
TNAHELSMVYAALAGDDDQMVAASRYAVLDDWRHLYDGNLLVILPDTYGTTSFLRNAPDWVADWTGARPDSKPPIAGGDE
FIDWWKANGRDPKEKLLVLSDGMDIDSIEQSFRHFKDRVRVSFGWGTNLTNDFRGCAPNGGNALDPISLVCKVVEAAGRP
AVKLSDNPTKATGPDADVGRYLQIFGTEGMTDHAVDV

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family [H]

Homologues:

Organism=Escherichia coli, GI1787162, Length=416, Percent_Identity=32.2115384615385, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6324783, Length=428, Percent_Identity=28.0373831775701, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638 [H]

Pfam domain/function: PF04095 NAPRTase [H]

EC number: =2.4.2.11 [H]

Molecular weight: Translated: 49589; Mature: 49458

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTDIATRVHNHNYRMDPVVRTLLDTDFYKLLMLQLIWMKHRDTPVTFGVINRSKSIRI
CCHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEE
ADEIDIGELRAQLDHARTLRLSKREMIWLAGNTFYGKKQIFRPEFLSWLETFALPEYEVD
ECCCCHHHHHHHHHHHHHHEECCCEEEEEECCCEECCHHCCCHHHHHHHHHHCCCCEEEE
VRNGQYELRFAGPWSLTTMWEIPALSIINELRARKAMRNMGRFELDVLYARAKAKLWGKV
ECCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
ERLQVLKAAGRLKVGDFGTRRRHGYLWQRWCCEALQEGIGDAFIGTSNVHVAMEIGSEAI
HHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCHHH
GTNAHELSMVYAALAGDDDQMVAASRYAVLDDWRHLYDGNLLVILPDTYGTTSFLRNAPD
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCH
WVADWTGARPDSKPPIAGGDEFIDWWKANGRDPKEKLLVLSDGMDIDSIEQSFRHFKDRV
HHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHE
RVSFGWGTNLTNDFRGCAPNGGNALDPISLVCKVVEAAGRPAVKLSDNPTKATGPDADVG
EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
RYLQIFGTEGMTDHAVDV
HEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SFTDIATRVHNHNYRMDPVVRTLLDTDFYKLLMLQLIWMKHRDTPVTFGVINRSKSIRI
CHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEE
ADEIDIGELRAQLDHARTLRLSKREMIWLAGNTFYGKKQIFRPEFLSWLETFALPEYEVD
ECCCCHHHHHHHHHHHHHHEECCCEEEEEECCCEECCHHCCCHHHHHHHHHHCCCCEEEE
VRNGQYELRFAGPWSLTTMWEIPALSIINELRARKAMRNMGRFELDVLYARAKAKLWGKV
ECCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
ERLQVLKAAGRLKVGDFGTRRRHGYLWQRWCCEALQEGIGDAFIGTSNVHVAMEIGSEAI
HHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCHHH
GTNAHELSMVYAALAGDDDQMVAASRYAVLDDWRHLYDGNLLVILPDTYGTTSFLRNAPD
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCH
WVADWTGARPDSKPPIAGGDEFIDWWKANGRDPKEKLLVLSDGMDIDSIEQSFRHFKDRV
HHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHE
RVSFGWGTNLTNDFRGCAPNGGNALDPISLVCKVVEAAGRPAVKLSDNPTKATGPDADVG
EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
RYLQIFGTEGMTDHAVDV
HEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]