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The map label for this gene is tig [H]

Identifier: 86749680

GI number: 86749680

Start: 2939672

End: 2941030

Strand: Direct

Name: tig [H]

Synonym: RPB_2562

Alternate gene names: 86749680

Gene position: 2939672-2941030 (Clockwise)

Preceding gene: 86749678

Following gene: 86749681

Centisome position: 55.14

GC content: 64.83

Gene sequence:

>1359_bases
ATGCAGGTCAAGGAAACCGTCGCCGACGGGTTGAAGCGCGAATTCGAAGTCAATGTCCCGGCTGCGGACATCGACGCCCA
GGTCGATGCGCGTCTGGTCGACCTGAAGGACAAGGTGAAGCTCAACGGCTTCCGTCCCGGCAAGGTCCCGGTCAGCCATC
TGAAGCGCGTCTACGGCCGCTCCGTCGCCGCCGAGACCATCGACAAGCTGGTCCGCGAGACCAATGACGGCATCTTCGCC
GAGCGCGGCTTCCGCCTCGCCACCGAGCCGAAGATCACGATGCCGCAGGACCAGAAAGTGGTCGAGGACATCCTCGCCGG
CAAGTCGGACCTGAATTACACCGTCGCGATCGAAGTGGTGCCGACCATCGAACTCGCCGACTTCAAGAGCTTCTCGGTCG
AGAAGCCGGTGGTCGAGGTTTCGGACTCCGACGTCGATGATGCGATCAAGCGGATCGCCGAGGCCAATCGCGCCTATGCC
GACAAGGCCGAGGGCGCCAAGGCTGAAAGCGGCGACCGGGTGACGATCTCGTTCAAGGGCTCGATCGAGGGCGTGCCGTT
CGACGGCGGCACCGGCGAGGACATCCCGGTGGTGATCGGCTCGAACAGCTTCATCCCGGGTTTCGAGGATCAGCTGATCG
GCATCGCCGTCGGCGAGACCCGCACCATCAAGGTGACGTTCCCGACCAACTATGCCAGCGAGACGCTGGCCGGCAAGCCG
GCCGAGTTCGAGACCACCGCGACCAAGGTCGAGGCGCCGCAGGACACCACGATCGATGACGAATTCGCCAAGACGCTCGG
CATGGAATCGCTCGACAAGCTGAAGGAAGCCGCCAAGGCGCGGCTCGCCGCGGAATACGCCGGCGCCACCCGGCTGCGCG
TCAAGCGCCAGCTGCTCGACCGCCTCGACGAAACCCACAAATTCGATGCGCCGCCGTCGCTGGTGGAGCAGGAATTCGCC
GTGATGTGGCAGTCGATCAACGCCGAGATGCAGCAGAACGGCAAGAGCTTCGCCGACGAGGACACCACCGAGGAGGCGGC
GCGCGAGGAATATCGCAAGATCGCCGACCGCCGCGTCCGCCTCGGCCTGGTGCTGTCGGAGATCGGCGAGAAGAACAAGA
TCCAGGTCACCGACGACGAGGTCAGCCGCGCGGTGATCGAGCGCGCCCGGCAGATGCCCGGCCGCGAGAAGGAAGTCTGG
GACTTCTACCGCAGCAACGCCGAGGCGCTGGCTCAGCTCCGCGCCCCGATCTACGAGGACAAGGTGGTCGATTTCATCCT
CGAACTCGCTACCGTGACCGAGAAGCCGGTCACCCGCGAGGAACTCTACAAGGACGACGACGACAAGACCGCCGCCTGA

Upstream 100 bases:

>100_bases
CACGCGCCCGCCAAGCCACCGGTGCAGGCGAGACGCCGCGCATCGCAACACTCGACACCTCCAGCCGCCCGGCTGGGTCA
CACGGAAGAAGATCGACGCC

Downstream 100 bases:

>100_bases
GCCAGTCTTCTGTAACTATTCCAACGGCCGCCGTGACCACCCGTCACGGCGGCCGTTCTGCGTCGTGAGGCGGCGTTAAC
GATGCGGCTTGAAAGCCGGG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQVKETVADGLKREFEVNVPAADIDAQVDARLVDLKDKVKLNGFRPGKVPVSHLKRVYGRSVAAETIDKLVRETNDGIFA
ERGFRLATEPKITMPQDQKVVEDILAGKSDLNYTVAIEVVPTIELADFKSFSVEKPVVEVSDSDVDDAIKRIAEANRAYA
DKAEGAKAESGDRVTISFKGSIEGVPFDGGTGEDIPVVIGSNSFIPGFEDQLIGIAVGETRTIKVTFPTNYASETLAGKP
AEFETTATKVEAPQDTTIDDEFAKTLGMESLDKLKEAAKARLAAEYAGATRLRVKRQLLDRLDETHKFDAPPSLVEQEFA
VMWQSINAEMQQNGKSFADEDTTEEAAREEYRKIADRRVRLGLVLSEIGEKNKIQVTDDEVSRAVIERARQMPGREKEVW
DFYRSNAEALAQLRAPIYEDKVVDFILELATVTEKPVTREELYKDDDDKTAA

Sequences:

>Translated_452_residues
MQVKETVADGLKREFEVNVPAADIDAQVDARLVDLKDKVKLNGFRPGKVPVSHLKRVYGRSVAAETIDKLVRETNDGIFA
ERGFRLATEPKITMPQDQKVVEDILAGKSDLNYTVAIEVVPTIELADFKSFSVEKPVVEVSDSDVDDAIKRIAEANRAYA
DKAEGAKAESGDRVTISFKGSIEGVPFDGGTGEDIPVVIGSNSFIPGFEDQLIGIAVGETRTIKVTFPTNYASETLAGKP
AEFETTATKVEAPQDTTIDDEFAKTLGMESLDKLKEAAKARLAAEYAGATRLRVKRQLLDRLDETHKFDAPPSLVEQEFA
VMWQSINAEMQQNGKSFADEDTTEEAAREEYRKIADRRVRLGLVLSEIGEKNKIQVTDDEVSRAVIERARQMPGREKEVW
DFYRSNAEALAQLRAPIYEDKVVDFILELATVTEKPVTREELYKDDDDKTAA
>Mature_452_residues
MQVKETVADGLKREFEVNVPAADIDAQVDARLVDLKDKVKLNGFRPGKVPVSHLKRVYGRSVAAETIDKLVRETNDGIFA
ERGFRLATEPKITMPQDQKVVEDILAGKSDLNYTVAIEVVPTIELADFKSFSVEKPVVEVSDSDVDDAIKRIAEANRAYA
DKAEGAKAESGDRVTISFKGSIEGVPFDGGTGEDIPVVIGSNSFIPGFEDQLIGIAVGETRTIKVTFPTNYASETLAGKP
AEFETTATKVEAPQDTTIDDEFAKTLGMESLDKLKEAAKARLAAEYAGATRLRVKRQLLDRLDETHKFDAPPSLVEQEFA
VMWQSINAEMQQNGKSFADEDTTEEAAREEYRKIADRRVRLGLVLSEIGEKNKIQVTDDEVSRAVIERARQMPGREKEVW
DFYRSNAEALAQLRAPIYEDKVVDFILELATVTEKPVTREELYKDDDDKTAA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=446, Percent_Identity=34.304932735426, Blast_Score=217, Evalue=1e-57,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 50112; Mature: 50112

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKETVADGLKREFEVNVPAADIDAQVDARLVDLKDKVKLNGFRPGKVPVSHLKRVYGR
CCCHHHHHHHHHHCEEECCCCCCCCCCCCCEEECCHHHHEECCCCCCCCCHHHHHHHHCC
SVAAETIDKLVRETNDGIFAERGFRLATEPKITMPQDQKVVEDILAGKSDLNYTVAIEVV
HHHHHHHHHHHHHCCCCEEHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
PTIELADFKSFSVEKPVVEVSDSDVDDAIKRIAEANRAYADKAEGAKAESGDRVTISFKG
ECEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
SIEGVPFDGGTGEDIPVVIGSNSFIPGFEDQLIGIAVGETRTIKVTFPTNYASETLAGKP
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHCCCC
AEFETTATKVEAPQDTTIDDEFAKTLGMESLDKLKEAAKARLAAEYAGATRLRVKRQLLD
CCCCHHHHEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLDETHKFDAPPSLVEQEFAVMWQSINAEMQQNGKSFADEDTTEEAAREEYRKIADRRVR
HHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGLVLSEIGEKNKIQVTDDEVSRAVIERARQMPGREKEVWDFYRSNAEALAQLRAPIYED
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCHHHH
KVVDFILELATVTEKPVTREELYKDDDDKTAA
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MQVKETVADGLKREFEVNVPAADIDAQVDARLVDLKDKVKLNGFRPGKVPVSHLKRVYGR
CCCHHHHHHHHHHCEEECCCCCCCCCCCCCEEECCHHHHEECCCCCCCCCHHHHHHHHCC
SVAAETIDKLVRETNDGIFAERGFRLATEPKITMPQDQKVVEDILAGKSDLNYTVAIEVV
HHHHHHHHHHHHHCCCCEEHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
PTIELADFKSFSVEKPVVEVSDSDVDDAIKRIAEANRAYADKAEGAKAESGDRVTISFKG
ECEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
SIEGVPFDGGTGEDIPVVIGSNSFIPGFEDQLIGIAVGETRTIKVTFPTNYASETLAGKP
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHCCCC
AEFETTATKVEAPQDTTIDDEFAKTLGMESLDKLKEAAKARLAAEYAGATRLRVKRQLLD
CCCCHHHHEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLDETHKFDAPPSLVEQEFAVMWQSINAEMQQNGKSFADEDTTEEAAREEYRKIADRRVR
HHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGLVLSEIGEKNKIQVTDDEVSRAVIERARQMPGREKEVWDFYRSNAEALAQLRAPIYED
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCHHHH
KVVDFILELATVTEKPVTREELYKDDDDKTAA
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA