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The map label for this gene is pleD [H]

Identifier: 86749533

GI number: 86749533

Start: 2765298

End: 2766671

Strand: Direct

Name: pleD [H]

Synonym: RPB_2413

Alternate gene names: 86749533

Gene position: 2765298-2766671 (Clockwise)

Preceding gene: 86749532

Following gene: 86749535

Centisome position: 51.87

GC content: 66.59

Gene sequence:

>1374_bases
ATGTCCGCACGCATTTTGGTGGTCGACGACATTCCGGCCAACGTCAAGCTGCTCGAAGCGCGGCTGTCGGCGGAGTATTT
CGACGTCGTCACCGCGGCCAACGGCGCGCAGGCGCTGGAGATCTGCGACCGCGCCGAATGCGACATCGTGCTGCTCGACG
TGATGATGCCGGACATGGACGGATTCGAGGTCTGCCGCCGGTTGAAGGCCAATCCGAAGACGCATTTCATCCCGGTGGTG
ATGGTGACGGCGCTCGACAGCCCGGCCGATCGGGTGCGCGGGCTCGAGGCCGGCGCCGACGATTTCCTCACCAAGCCGGT
GTCGGATGTGGTGCTGATCGCGCGGGTGCGTTCGCTGACGCGGCTGAAGATGATGACCGACGAATTGCGCATGCGCGCGA
TCACCTCGTTCGAGATCGGCGTGCAGGCGCCGGAGCGCGAGGCGGTCAACGATCTCGGCAAAGGCGGCCGCATCCTGCTG
GTCGACGACCGGCCGTCGTCCTACGAGCGGCTGGCGCCCTTGCTCGCCGCCGAACACGACATCGACGTCGAACCCAATCC
GTCCGAGGCGCTGTTCCATGCGGCCGAAGGCAATTACGATCTGCTGATCGTGTCGCTCGGCCTGGAGAATTTCGACGGGC
TGCGGCTGTGCAGCCAGGCCCGTTCGCTGGAGCGGACACGCCACGTGCCGATCCTCGCCATCGCCGACGCCGACAACAAT
GCGCGGCTGCTGCGCGGGCTCGAGATCGGCGTCAACGACTACCTGCTGCGGCCGGTCGACAAGAACGAACTCTTGGCGCG
CGCGCGCACCCAGATCCGCCGTCGGCGCTACACCGATCATCTGCGCGACAACGTGCAGCATTCGATCGAGATGGCGATCA
CCGACGGCCTGACCGGGCTGCACAATCGCCGCTACATGGAGAGCCATCTCGCGACGCTGGCCGAACAGGCGGGGTCGCGC
GGCAAGCCGCTGGCGCTGATGATCCTCGACATCGACTTCTTCAAGGCGATCAACGACACCTACGGCCACGATGCCGGCGA
CGACGTGCTGCGCGAATTCGCGGTGCGGATCAAGAAGTCGATCCGCGGCATCGATCTGGCCTGCCGCTATGGCGGCGAGG
AGTTCGTGATCGTGATGCCGGAGACCGACCTGCAGGTCGCCCAGATGGTGGCGGAGCGGCTGCGCCGCGCCATCGCCGGC
GAACCGTTCGCGATCGAGAAGGGGACGAAGCGGATCGCCGTCACGATCTCGATCGGCCTGTCGACGCTCGAACGCAAGGG
AGAAGCGGTGCGCGATCTCCTCAAGCGCGCCGACACCGCGCTGTACCGCGCCAAGCACGACGGCCGGAATCGGGTGGTCG
CGGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
ACGCATCCGCGAGGGCGGCTGCGAAGCCTATCTGTCGAAGCCGATATCGGTCGGCAAGTTCATCGAAACCGTGCGCCGTT
TCATCGGATAGGAGAACGCG

Downstream 100 bases:

>100_bases
GCACTGGCACCCTCTGCATCGAAACACGCGCCGCCGTCATCCTGAGGTGCGCGCCGTCCTTCACGGCGCGCCTCGAAGGA
TGGCCGCGCGTGCCTGCCGC

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSARILVVDDIPANVKLLEARLSAEYFDVVTAANGAQALEICDRAECDIVLLDVMMPDMDGFEVCRRLKANPKTHFIPVV
MVTALDSPADRVRGLEAGADDFLTKPVSDVVLIARVRSLTRLKMMTDELRMRAITSFEIGVQAPEREAVNDLGKGGRILL
VDDRPSSYERLAPLLAAEHDIDVEPNPSEALFHAAEGNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAIADADNN
ARLLRGLEIGVNDYLLRPVDKNELLARARTQIRRRRYTDHLRDNVQHSIEMAITDGLTGLHNRRYMESHLATLAEQAGSR
GKPLALMILDIDFFKAINDTYGHDAGDDVLREFAVRIKKSIRGIDLACRYGGEEFVIVMPETDLQVAQMVAERLRRAIAG
EPFAIEKGTKRIAVTISIGLSTLERKGEAVRDLLKRADTALYRAKHDGRNRVVAAAA

Sequences:

>Translated_457_residues
MSARILVVDDIPANVKLLEARLSAEYFDVVTAANGAQALEICDRAECDIVLLDVMMPDMDGFEVCRRLKANPKTHFIPVV
MVTALDSPADRVRGLEAGADDFLTKPVSDVVLIARVRSLTRLKMMTDELRMRAITSFEIGVQAPEREAVNDLGKGGRILL
VDDRPSSYERLAPLLAAEHDIDVEPNPSEALFHAAEGNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAIADADNN
ARLLRGLEIGVNDYLLRPVDKNELLARARTQIRRRRYTDHLRDNVQHSIEMAITDGLTGLHNRRYMESHLATLAEQAGSR
GKPLALMILDIDFFKAINDTYGHDAGDDVLREFAVRIKKSIRGIDLACRYGGEEFVIVMPETDLQVAQMVAERLRRAIAG
EPFAIEKGTKRIAVTISIGLSTLERKGEAVRDLLKRADTALYRAKHDGRNRVVAAAA
>Mature_456_residues
SARILVVDDIPANVKLLEARLSAEYFDVVTAANGAQALEICDRAECDIVLLDVMMPDMDGFEVCRRLKANPKTHFIPVVM
VTALDSPADRVRGLEAGADDFLTKPVSDVVLIARVRSLTRLKMMTDELRMRAITSFEIGVQAPEREAVNDLGKGGRILLV
DDRPSSYERLAPLLAAEHDIDVEPNPSEALFHAAEGNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAIADADNNA
RLLRGLEIGVNDYLLRPVDKNELLARARTQIRRRRYTDHLRDNVQHSIEMAITDGLTGLHNRRYMESHLATLAEQAGSRG
KPLALMILDIDFFKAINDTYGHDAGDDVLREFAVRIKKSIRGIDLACRYGGEEFVIVMPETDLQVAQMVAERLRRAIAGE
PFAIEKGTKRIAVTISIGLSTLERKGEAVRDLLKRADTALYRAKHDGRNRVVAAAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=40.5714285714286, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=35.5421686746988, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=39.8843930635838, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=39.3939393939394, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=38.6075949367089, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=36.6459627329193, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=34.1772151898734, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=39.8305084745763, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=32.7160493827161, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=36.4406779661017, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=39.6694214876033, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=38.3333333333333, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI2367329, Length=125, Percent_Identity=42.4, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=33.1428571428571, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=36.094674556213, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=34.7457627118644, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI145693157, Length=108, Percent_Identity=41.6666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=33.6134453781513, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789149, Length=109, Percent_Identity=34.8623853211009, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=38.135593220339, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790863, Length=163, Percent_Identity=26.9938650306748, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788713, Length=173, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081977, Length=187, Percent_Identity=28.3422459893048, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=41.3461538461538, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789402, Length=118, Percent_Identity=35.5932203389831, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=116, Percent_Identity=37.0689655172414, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788905, Length=136, Percent_Identity=37.5, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790552, Length=121, Percent_Identity=36.3636363636364, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI145693140, Length=111, Percent_Identity=35.1351351351351, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI1788550, Length=155, Percent_Identity=28.3870967741935, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50748; Mature: 50617

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARILVVDDIPANVKLLEARLSAEYFDVVTAANGAQALEICDRAECDIVLLDVMMPDMD
CCCEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEEEECCCCCC
GFEVCRRLKANPKTHFIPVVMVTALDSPADRVRGLEAGADDFLTKPVSDVVLIARVRSLT
HHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKMMTDELRMRAITSFEIGVQAPEREAVNDLGKGGRILLVDDRPSSYERLAPLLAAEHD
HHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCC
IDVEPNPSEALFHAAEGNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAIADADNN
CCCCCCCCHHEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
ARLLRGLEIGVNDYLLRPVDKNELLARARTQIRRRRYTDHLRDNVQHSIEMAITDGLTGL
CEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
HNRRYMESHLATLAEQAGSRGKPLALMILDIDFFKAINDTYGHDAGDDVLREFAVRIKKS
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
IRGIDLACRYGGEEFVIVMPETDLQVAQMVAERLRRAIAGEPFAIEKGTKRIAVTISIGL
HCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEECH
STLERKGEAVRDLLKRADTALYRAKHDGRNRVVAAAA
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
SARILVVDDIPANVKLLEARLSAEYFDVVTAANGAQALEICDRAECDIVLLDVMMPDMD
CCEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEEEECCCCCC
GFEVCRRLKANPKTHFIPVVMVTALDSPADRVRGLEAGADDFLTKPVSDVVLIARVRSLT
HHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKMMTDELRMRAITSFEIGVQAPEREAVNDLGKGGRILLVDDRPSSYERLAPLLAAEHD
HHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCC
IDVEPNPSEALFHAAEGNYDLLIVSLGLENFDGLRLCSQARSLERTRHVPILAIADADNN
CCCCCCCCHHEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
ARLLRGLEIGVNDYLLRPVDKNELLARARTQIRRRRYTDHLRDNVQHSIEMAITDGLTGL
CEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
HNRRYMESHLATLAEQAGSRGKPLALMILDIDFFKAINDTYGHDAGDDVLREFAVRIKKS
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
IRGIDLACRYGGEEFVIVMPETDLQVAQMVAERLRRAIAGEPFAIEKGTKRIAVTISIGL
HCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEECH
STLERKGEAVRDLLKRADTALYRAKHDGRNRVVAAAA
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA