BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is dkgB [H]

Identifier: 86749501

GI number: 86749501

Start: 2734722

End: 2735540

Strand: Direct

Name: dkgB [H]

Synonym: RPB_2381

Alternate gene names: 86749501

Gene position: 2734722-2735540 (Clockwise)

Preceding gene: 86749500

Following gene: 86749502

Centisome position: 51.29

GC content: 64.84

Gene sequence:

>819_bases
ATGCAGTTCGTTGAAGCCCATGGCGCGAAAATTCCCGCGATCGGACTCGGCACCTGGGAGCTGAGCGGCCATTCCTGCGC
CCGCATCGTCGAGCAGGCGATCCGGCTCGGCTATCGCCACATCGACACCGCGCAGGTCTACTACAACGAACGCGACGTCG
GCGAGGGCATCCGCAATTCGCACATCAGGCGCAGCGAGATGTTCGTCGCCACCAAGGTCTGGACGACGCATTTCGCGCCC
AACGATCTGCTCCGCTCGACCAAAGAGAGCCTGCACAAGATGCGGCTGTCGGAGGTCGACCTGCTGATGCTGCACTGGCC
GAACGCGCATGTGCCGCTGAACGAAACGCTCGGCGCACTCGCGCACGCCAGAGAACTCGGCATGACGCGGCATATCGGCG
TGTCGAATTTCACCGTGGCGCTGCTGAAGCAGGCGGTCGCGCTCTGTCCGGAACCGCTGGTGTGCAATCAGGTCGAGTAT
CATCCGTTTCTCGGCCAGAGCAATTTGCTGGCGGCCTGCGCCGAACTCGGCATCGCGATGGTCGCCTACAGCCCGATCGC
CAAGGGCACCGCGCGGCAGAGCGAGGTGCTGACCCGGATCGGGGCAGCGCACGGCAAGTCGCCCTCGCAGGTGTGCCTGC
GCTGGCTGGTGCAGCAGAACGTTCCGGCGATCCCGCGCACCTCGCGGATCGAGCGCCTCTCGCAAAACATCGACGTCTTC
GACTTCGTGCTCAGCGACGACGAGATGCGCGAGATCTTTGCGCTGGGCAGCCCCGAGGGCCGGCTGGTGCATTCGGTCAC
CGAGCCGGATTGGGACTGA

Upstream 100 bases:

>100_bases
CTGCGGATTCGACGGCCTCGTAATCGCGGCGATTGGCACGGCGGCAATCCCGGGACATACTGCGCAGACGGATCTGCGTT
GCGACTGCGAGAGCACCGAT

Downstream 100 bases:

>100_bases
GCGACATCGCGGCAGATTGACCCGGCCGGCGAAATCACGCACCTCGCGGGGCATGGCCTCGCTGCGCGGGTGATCGATGT
TCTTCGTGCTGTCCAAAATC

Product: 2,5-didehydrogluconate reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MQFVEAHGAKIPAIGLGTWELSGHSCARIVEQAIRLGYRHIDTAQVYYNERDVGEGIRNSHIRRSEMFVATKVWTTHFAP
NDLLRSTKESLHKMRLSEVDLLMLHWPNAHVPLNETLGALAHARELGMTRHIGVSNFTVALLKQAVALCPEPLVCNQVEY
HPFLGQSNLLAACAELGIAMVAYSPIAKGTARQSEVLTRIGAAHGKSPSQVCLRWLVQQNVPAIPRTSRIERLSQNIDVF
DFVLSDDEMREIFALGSPEGRLVHSVTEPDWD

Sequences:

>Translated_272_residues
MQFVEAHGAKIPAIGLGTWELSGHSCARIVEQAIRLGYRHIDTAQVYYNERDVGEGIRNSHIRRSEMFVATKVWTTHFAP
NDLLRSTKESLHKMRLSEVDLLMLHWPNAHVPLNETLGALAHARELGMTRHIGVSNFTVALLKQAVALCPEPLVCNQVEY
HPFLGQSNLLAACAELGIAMVAYSPIAKGTARQSEVLTRIGAAHGKSPSQVCLRWLVQQNVPAIPRTSRIERLSQNIDVF
DFVLSDDEMREIFALGSPEGRLVHSVTEPDWD
>Mature_272_residues
MQFVEAHGAKIPAIGLGTWELSGHSCARIVEQAIRLGYRHIDTAQVYYNERDVGEGIRNSHIRRSEMFVATKVWTTHFAP
NDLLRSTKESLHKMRLSEVDLLMLHWPNAHVPLNETLGALAHARELGMTRHIGVSNFTVALLKQAVALCPEPLVCNQVEY
HPFLGQSNLLAACAELGIAMVAYSPIAKGTARQSEVLTRIGAAHGKSPSQVCLRWLVQQNVPAIPRTSRIERLSQNIDVF
DFVLSDDEMREIFALGSPEGRLVHSVTEPDWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=284, Percent_Identity=32.3943661971831, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI5174391, Length=284, Percent_Identity=32.3943661971831, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI93277124, Length=296, Percent_Identity=32.4324324324324, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI223468663, Length=291, Percent_Identity=33.6769759450172, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5174695, Length=295, Percent_Identity=32.5423728813559, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=31.2714776632302, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI4502049, Length=284, Percent_Identity=32.0422535211268, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI310109922, Length=272, Percent_Identity=31.25, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI45446745, Length=291, Percent_Identity=30.5841924398625, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4503285, Length=291, Percent_Identity=30.5841924398625, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI310109920, Length=291, Percent_Identity=30.2405498281787, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI24497583, Length=290, Percent_Identity=30, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI300116271, Length=273, Percent_Identity=31.1355311355311, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=31.7689530685921, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI5453543, Length=291, Percent_Identity=29.553264604811, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=31.9391634980989, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI310109924, Length=153, Percent_Identity=32.6797385620915, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI27436969, Length=204, Percent_Identity=26.9607843137255, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4504825, Length=204, Percent_Identity=26.9607843137255, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI27436966, Length=204, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27436962, Length=236, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27436964, Length=204, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI207028673, Length=120, Percent_Identity=32.5, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI310109926, Length=120, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI27436971, Length=204, Percent_Identity=24.5098039215686, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786400, Length=271, Percent_Identity=37.6383763837638, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI87082198, Length=252, Percent_Identity=32.9365079365079, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788081, Length=272, Percent_Identity=31.6176470588235, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787674, Length=269, Percent_Identity=27.8810408921933, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081735, Length=287, Percent_Identity=26.8292682926829, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1789375, Length=313, Percent_Identity=24.2811501597444, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI48994888, Length=162, Percent_Identity=29.0123456790123, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=267, Percent_Identity=33.3333333333333, Blast_Score=170, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17537077, Length=279, Percent_Identity=35.1254480286738, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17537075, Length=279, Percent_Identity=35.1254480286738, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537079, Length=288, Percent_Identity=30.5555555555556, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17566692, Length=281, Percent_Identity=27.7580071174377, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550248, Length=280, Percent_Identity=29.2857142857143, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=269, Percent_Identity=30.8550185873606, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17564128, Length=285, Percent_Identity=29.8245614035088, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17561298, Length=265, Percent_Identity=27.1698113207547, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17538386, Length=275, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=26.3736263736264, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17562292, Length=276, Percent_Identity=26.4492753623188, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321896, Length=300, Percent_Identity=32, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324694, Length=296, Percent_Identity=32.7702702702703, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320576, Length=287, Percent_Identity=30.3135888501742, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322556, Length=256, Percent_Identity=30.859375, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319625, Length=308, Percent_Identity=29.2207792207792, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320079, Length=281, Percent_Identity=29.1814946619217, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24644950, Length=291, Percent_Identity=32.6460481099656, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24657054, Length=278, Percent_Identity=32.3741007194245, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221378297, Length=311, Percent_Identity=30.5466237942122, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24663317, Length=275, Percent_Identity=33.0909090909091, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21356425, Length=274, Percent_Identity=31.7518248175182, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI20129731, Length=270, Percent_Identity=34.8148148148148, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553081, Length=277, Percent_Identity=29.2418772563177, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24662781, Length=273, Percent_Identity=32.6007326007326, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24662789, Length=299, Percent_Identity=28.0936454849498, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24662785, Length=272, Percent_Identity=32.7205882352941, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281366140, Length=248, Percent_Identity=28.6290322580645, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 30301; Mature: 30301

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFVEAHGAKIPAIGLGTWELSGHSCARIVEQAIRLGYRHIDTAQVYYNERDVGEGIRNS
CCCCCCCCCCCCEEECCEEEECCHHHHHHHHHHHHHCHHCCCHHHEEECCHHHHHHHHHH
HIRRSEMFVATKVWTTHFAPNDLLRSTKESLHKMRLSEVDLLMLHWPNAHVPLNETLGAL
CCHHHHHEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHH
AHARELGMTRHIGVSNFTVALLKQAVALCPEPLVCNQVEYHPFLGQSNLLAACAELGIAM
HHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VAYSPIAKGTARQSEVLTRIGAAHGKSPSQVCLRWLVQQNVPAIPRTSRIERLSQNIDVF
HHHCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEE
DFVLSDDEMREIFALGSPEGRLVHSVTEPDWD
EEEECCHHHHHHHHCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MQFVEAHGAKIPAIGLGTWELSGHSCARIVEQAIRLGYRHIDTAQVYYNERDVGEGIRNS
CCCCCCCCCCCCEEECCEEEECCHHHHHHHHHHHHHCHHCCCHHHEEECCHHHHHHHHHH
HIRRSEMFVATKVWTTHFAPNDLLRSTKESLHKMRLSEVDLLMLHWPNAHVPLNETLGAL
CCHHHHHEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHH
AHARELGMTRHIGVSNFTVALLKQAVALCPEPLVCNQVEYHPFLGQSNLLAACAELGIAM
HHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VAYSPIAKGTARQSEVLTRIGAAHGKSPSQVCLRWLVQQNVPAIPRTSRIERLSQNIDVF
HHHCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEE
DFVLSDDEMREIFALGSPEGRLVHSVTEPDWD
EEEECCHHHHHHHHCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]