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The map label for this gene is glgX [H]

Identifier: 86749374

GI number: 86749374

Start: 2600267

End: 2602468

Strand: Reverse

Name: glgX [H]

Synonym: RPB_2254

Alternate gene names: 86749374

Gene position: 2602468-2600267 (Counterclockwise)

Preceding gene: 86749375

Following gene: 86749373

Centisome position: 48.81

GC content: 63.76

Gene sequence:

>2202_bases
ATGACCACCGACACTCTCGATCCGCCGCCGCCGACCACTGCGGCGACCGCCGAATCGAAGCAGCCGCCGAAACCCGCGCC
GGCCTACAAGATCATCAACGAGACCGGCAACGTACGCGAAGGCTTGCCCTACCCGCTCGGCGCCTGCTGGGATGGCGAAG
GCACCAACTTCGCGCTGTTCTCGGCAAACGCCACCAAGGTCGAGGTCTGCCTGTTCGACGGCGACACCGAGCGCCGGATC
GAGTTGCCCGAGTACACCGACGAAGTCTTCCACGGCTACATACCTGATATCGGCCCCGGCACGTTCTACGGCTATCGTGT
GCACGGCCCCTACGAGCCGGAGAACGGTCACCGCTTCAACCCGAACAAGCTGCTGCTCGACCCTTACGCACGCGCGCACG
CCGGCGAGCTGAAGTGGGACCCGGCGGTGTTCGGCTATCAGATGGAAACCTGCGACGACACCACCTTCGACGAACGCGAC
TCCGCGGCGTTCGTGCCGAAATGCGTCGTCGTCGATCCGGGCTTCGACTGGAAGGGCGAGCCCGGCCGCAAATTCGTGCC
GTGGGATCAGACCATCGTCTACGAAACCCACGTCAAGGGTTTCACCAAGCTGCATCCGGAGGTGCCGGAACATCTGCGCG
GAACCTATGCCGGCTTCGGCAGCCAGCCGGTGGTGGACTACATCGCCGCACTCGGCGTCACCGCCGTCGAGCTGCTGCCG
ATCCACACCTTCGTCAACGACAGCTATCTGCTCGACAAGAAGCTGGTGAACTATTGGGGTTACAACACCATCGGCTTCTT
CGCGCCCGATCCGCGCTACGCGGCGGACGTGCCGAACAGCCTGCGCGAATTCAAGGAGATGGTGGCGCGGCTGCACGACG
TCGGCCTCGCGGTGATCCTCGACGTCGTCTACAACCACACCGCCGAGGGCAACGAACTCGGCCCGACGCTGTCATTCAAA
GGCATCGACAACGCCAGCTACTATCGCCTGCTGCCGGAGCAGGAGCGCTATTACATCAACGACACCGGCACCGGCAACAC
CGTCAACCTCACGCATCCGCGCGTGATCCAGATGGTCACCGACTCGCTTCGCTACTGGGCCGGCGAGATGCACATCGACG
GATTCCGCTTCGATCTCGGCACCATCCTGGCCCGCGAGGTTCACGGCTTCGACGACCAGAGCGGGTTCCTCAAGGCGGTC
AATCAGGACCCGATGCTGAGCTCGGTGAAGCTGATCGCAGAACCGTGGGATTGCGGACCAGGCGGCTATCAGGTCGGTGG
ATTTCCGCCCGGCTGGGCGGAATGGAACGACAAGTTCCGTGATACGATTCGCGACTACTGGCGCGGCGACGCTTCGGTGT
CGGCCCTCGCGCCACGTCTGGCCGCCTCCGGCGACGTGTTCAATCGGCAGGGCCGCCGAGCCTGGGCCAGCGTCAACTTC
ATCACGGCGCATGACGGCTTCACGCTCAACGACTGGGCGAGCTACAACGAGAAGCACAACATTGCCAACGGCGAGGACGG
GAAGGACGGTCATTCCGACAACCGGTCGTGGAATTGCGGCGCCGAGGGACCGACCGACGACGAGGCGATCCTCGCGCTGC
GCGAGCGCCAGAAGCGCAACATGCTGGCGACGCTGCTGCTGGCGCAAGGCACCCCGATGCTGCTCGCCGGTGACGAATTC
GGTCGCACCCAGCAGGGTAACAACAACGCCTATTGCCAGGACAACGAGATCTCCTGGGTGCATTGGGACCACGACGCAGC
CGCGCAGGATCTCCTGACCTTCACCAAACGGTTGATCGAACTGCGGCGGACCTGTCCGACGCTGCGACGCAGCCGTTTCC
TGCAGGGCCAATACGACGAGGCGCTCGACGTCTGCGACGTCACCTGGATCAACGCCAATGGTGGTCCGATGCAGACCGAG
CACTGGGAAGACGGCAACGTGAAATGCTTCGGCATGTTGCTGGACGGCCGCGCGCAGAAGACCGGCATACGCCGGCACGC
AGAGGACGACACCGTGTTGCTGATCACCAACGCCTTCGACGGCGTGGTCGACTTCAACCTGCCCGAGGTTCCGCACGGCT
CGAAATGGCAGTTGCTGATCGACACCAATCTTCCGGACGGAGCCGCCGGCGAACAGTTCGCATGCGGAGCTGCGTATCAG
GTGACGGCGCGATCGGTCCTGCTGTTCGGATTGGTGGAGTAG

Upstream 100 bases:

>100_bases
CGGTCGACAGAATGGGGGACGACATCATGAGAGATCGATCGGCATCGGCGGCGCACACGGCCGCCTGCGTGACAACGCGG
CAGCATCCGGGGGCGCGACG

Downstream 100 bases:

>100_bases
AGTTCCGCTTCTCGTCGTCAGACCGGAACTTCAGCTCGAAGGCGCTTCGTCCCGCGGGCCGCTGCCAGTTCCTTCGATGG
CGAGGAAACGCCCACTCTGC

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MTTDTLDPPPPTTAATAESKQPPKPAPAYKIINETGNVREGLPYPLGACWDGEGTNFALFSANATKVEVCLFDGDTERRI
ELPEYTDEVFHGYIPDIGPGTFYGYRVHGPYEPENGHRFNPNKLLLDPYARAHAGELKWDPAVFGYQMETCDDTTFDERD
SAAFVPKCVVVDPGFDWKGEPGRKFVPWDQTIVYETHVKGFTKLHPEVPEHLRGTYAGFGSQPVVDYIAALGVTAVELLP
IHTFVNDSYLLDKKLVNYWGYNTIGFFAPDPRYAADVPNSLREFKEMVARLHDVGLAVILDVVYNHTAEGNELGPTLSFK
GIDNASYYRLLPEQERYYINDTGTGNTVNLTHPRVIQMVTDSLRYWAGEMHIDGFRFDLGTILAREVHGFDDQSGFLKAV
NQDPMLSSVKLIAEPWDCGPGGYQVGGFPPGWAEWNDKFRDTIRDYWRGDASVSALAPRLAASGDVFNRQGRRAWASVNF
ITAHDGFTLNDWASYNEKHNIANGEDGKDGHSDNRSWNCGAEGPTDDEAILALRERQKRNMLATLLLAQGTPMLLAGDEF
GRTQQGNNNAYCQDNEISWVHWDHDAAAQDLLTFTKRLIELRRTCPTLRRSRFLQGQYDEALDVCDVTWINANGGPMQTE
HWEDGNVKCFGMLLDGRAQKTGIRRHAEDDTVLLITNAFDGVVDFNLPEVPHGSKWQLLIDTNLPDGAAGEQFACGAAYQ
VTARSVLLFGLVE

Sequences:

>Translated_733_residues
MTTDTLDPPPPTTAATAESKQPPKPAPAYKIINETGNVREGLPYPLGACWDGEGTNFALFSANATKVEVCLFDGDTERRI
ELPEYTDEVFHGYIPDIGPGTFYGYRVHGPYEPENGHRFNPNKLLLDPYARAHAGELKWDPAVFGYQMETCDDTTFDERD
SAAFVPKCVVVDPGFDWKGEPGRKFVPWDQTIVYETHVKGFTKLHPEVPEHLRGTYAGFGSQPVVDYIAALGVTAVELLP
IHTFVNDSYLLDKKLVNYWGYNTIGFFAPDPRYAADVPNSLREFKEMVARLHDVGLAVILDVVYNHTAEGNELGPTLSFK
GIDNASYYRLLPEQERYYINDTGTGNTVNLTHPRVIQMVTDSLRYWAGEMHIDGFRFDLGTILAREVHGFDDQSGFLKAV
NQDPMLSSVKLIAEPWDCGPGGYQVGGFPPGWAEWNDKFRDTIRDYWRGDASVSALAPRLAASGDVFNRQGRRAWASVNF
ITAHDGFTLNDWASYNEKHNIANGEDGKDGHSDNRSWNCGAEGPTDDEAILALRERQKRNMLATLLLAQGTPMLLAGDEF
GRTQQGNNNAYCQDNEISWVHWDHDAAAQDLLTFTKRLIELRRTCPTLRRSRFLQGQYDEALDVCDVTWINANGGPMQTE
HWEDGNVKCFGMLLDGRAQKTGIRRHAEDDTVLLITNAFDGVVDFNLPEVPHGSKWQLLIDTNLPDGAAGEQFACGAAYQ
VTARSVLLFGLVE
>Mature_732_residues
TTDTLDPPPPTTAATAESKQPPKPAPAYKIINETGNVREGLPYPLGACWDGEGTNFALFSANATKVEVCLFDGDTERRIE
LPEYTDEVFHGYIPDIGPGTFYGYRVHGPYEPENGHRFNPNKLLLDPYARAHAGELKWDPAVFGYQMETCDDTTFDERDS
AAFVPKCVVVDPGFDWKGEPGRKFVPWDQTIVYETHVKGFTKLHPEVPEHLRGTYAGFGSQPVVDYIAALGVTAVELLPI
HTFVNDSYLLDKKLVNYWGYNTIGFFAPDPRYAADVPNSLREFKEMVARLHDVGLAVILDVVYNHTAEGNELGPTLSFKG
IDNASYYRLLPEQERYYINDTGTGNTVNLTHPRVIQMVTDSLRYWAGEMHIDGFRFDLGTILAREVHGFDDQSGFLKAVN
QDPMLSSVKLIAEPWDCGPGGYQVGGFPPGWAEWNDKFRDTIRDYWRGDASVSALAPRLAASGDVFNRQGRRAWASVNFI
TAHDGFTLNDWASYNEKHNIANGEDGKDGHSDNRSWNCGAEGPTDDEAILALRERQKRNMLATLLLAQGTPMLLAGDEFG
RTQQGNNNAYCQDNEISWVHWDHDAAAQDLLTFTKRLIELRRTCPTLRRSRFLQGQYDEALDVCDVTWINANGGPMQTEH
WEDGNVKCFGMLLDGRAQKTGIRRHAEDDTVLLITNAFDGVVDFNLPEVPHGSKWQLLIDTNLPDGAAGEQFACGAAYQV
TARSVLLFGLVE

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=620, Percent_Identity=47.741935483871, Blast_Score=560, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 81811; Mature: 81680

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTLDPPPPTTAATAESKQPPKPAPAYKIINETGNVREGLPYPLGACWDGEGTNFALF
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCEEECCCCCEEEEE
SANATKVEVCLFDGDTERRIELPEYTDEVFHGYIPDIGPGTFYGYRVHGPYEPENGHRFN
ECCCCEEEEEEECCCCCCEEECCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEC
PNKLLLDPYARAHAGELKWDPAVFGYQMETCDDTTFDERDSAAFVPKCVVVDPGFDWKGE
CCEEEECCHHHCCCCCCCCCCCEECEEEECCCCCCCCCCCCCEECCEEEEECCCCCCCCC
PGRKFVPWDQTIVYETHVKGFTKLHPEVPEHLRGTYAGFGSQPVVDYIAALGVTAVELLP
CCCEECCCCCEEEEEECCCCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEE
IHTFVNDSYLLDKKLVNYWGYNTIGFFAPDPRYAADVPNSLREFKEMVARLHDVGLAVIL
EHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
DVVYNHTAEGNELGPTLSFKGIDNASYYRLLPEQERYYINDTGTGNTVNLTHPRVIQMVT
HHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCEEEECCCCCCEEECCCCHHHHHHH
DSLRYWAGEMHIDGFRFDLGTILAREVHGFDDQSGFLKAVNQDPMLSSVKLIAEPWDCGP
HHHHHHCCCEEECCEEEHHHHHHHHHHCCCCCCCCHHEECCCCCCHHHHHHEECCCCCCC
GGYQVGGFPPGWAEWNDKFRDTIRDYWRGDASVSALAPRLAASGDVFNRQGRRAWASVNF
CCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHCCCCEEEEEEEE
ITAHDGFTLNDWASYNEKHNIANGEDGKDGHSDNRSWNCGAEGPTDDEAILALRERQKRN
EEECCCEEECHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHH
MLATLLLAQGTPMLLAGDEFGRTQQGNNNAYCQDNEISWVHWDHDAAAQDLLTFTKRLIE
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHH
LRRTCPTLRRSRFLQGQYDEALDVCDVTWINANGGPMQTEHWEDGNVKCFGMLLDGRAQK
HHHHCHHHHHHHHCCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCHH
TGIRRHAEDDTVLLITNAFDGVVDFNLPEVPHGSKWQLLIDTNLPDGAAGEQFACGAAYQ
HHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCHHH
VTARSVLLFGLVE
HHHHEEEEEEECC
>Mature Secondary Structure 
TTDTLDPPPPTTAATAESKQPPKPAPAYKIINETGNVREGLPYPLGACWDGEGTNFALF
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCEEECCCCCEEEEE
SANATKVEVCLFDGDTERRIELPEYTDEVFHGYIPDIGPGTFYGYRVHGPYEPENGHRFN
ECCCCEEEEEEECCCCCCEEECCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEC
PNKLLLDPYARAHAGELKWDPAVFGYQMETCDDTTFDERDSAAFVPKCVVVDPGFDWKGE
CCEEEECCHHHCCCCCCCCCCCEECEEEECCCCCCCCCCCCCEECCEEEEECCCCCCCCC
PGRKFVPWDQTIVYETHVKGFTKLHPEVPEHLRGTYAGFGSQPVVDYIAALGVTAVELLP
CCCEECCCCCEEEEEECCCCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEE
IHTFVNDSYLLDKKLVNYWGYNTIGFFAPDPRYAADVPNSLREFKEMVARLHDVGLAVIL
EHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
DVVYNHTAEGNELGPTLSFKGIDNASYYRLLPEQERYYINDTGTGNTVNLTHPRVIQMVT
HHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCEEEECCCCCCEEECCCCHHHHHHH
DSLRYWAGEMHIDGFRFDLGTILAREVHGFDDQSGFLKAVNQDPMLSSVKLIAEPWDCGP
HHHHHHCCCEEECCEEEHHHHHHHHHHCCCCCCCCHHEECCCCCCHHHHHHEECCCCCCC
GGYQVGGFPPGWAEWNDKFRDTIRDYWRGDASVSALAPRLAASGDVFNRQGRRAWASVNF
CCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHCCCCEEEEEEEE
ITAHDGFTLNDWASYNEKHNIANGEDGKDGHSDNRSWNCGAEGPTDDEAILALRERQKRN
EEECCCEEECHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHH
MLATLLLAQGTPMLLAGDEFGRTQQGNNNAYCQDNEISWVHWDHDAAAQDLLTFTKRLIE
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHH
LRRTCPTLRRSRFLQGQYDEALDVCDVTWINANGGPMQTEHWEDGNVKCFGMLLDGRAQK
HHHHCHHHHHHHHCCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCHH
TGIRRHAEDDTVLLITNAFDGVVDFNLPEVPHGSKWQLLIDTNLPDGAAGEQFACGAAYQ
HHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCHHH
VTARSVLLFGLVE
HHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]