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The map label for this gene is cspE [C]

Identifier: 86749297

GI number: 86749297

Start: 2470302

End: 2470511

Strand: Reverse

Name: cspE [C]

Synonym: RPB_2176

Alternate gene names: 86749297

Gene position: 2470511-2470302 (Counterclockwise)

Preceding gene: 86749299

Following gene: 86749296

Centisome position: 46.34

GC content: 59.05

Gene sequence:

>210_bases
ATGCCGACAGGGACCGTGAAGTGGTTCAATGGACAAAAGGGCTTCGGCTTTATCGCGCCGAACGACGGTGGCAACGATGT
GTTCGTGCACATCAGCGCCGTTGAGCGCGCCGGGCTTTCGGGCCTCGCCGAAGGCCAGAAGGTCAATTTCGAGCTCAAGA
CCGACAAGATGCGCGGTAAGACGAGCGCGGAAAATCTCGAGCTGGTCTGA

Upstream 100 bases:

>100_bases
GAACTTTCTGAACGCGCCCGCGGGCTCCTTCCCAAAGACATCGATTAGTTGAACGACGGCGTGTGATCGTCACGCGTCTA
TATGCTTTGGAGAAGACAAG

Downstream 100 bases:

>100_bases
TCCAGCTCGCTCCGTTTCACGGATGTACTGAAACGGTAGGTGTGACCCGCTCGATCCTTTGCGATTGGGCGGGCGTCGCC
ATGTCTGCAGTCGGCTCATC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MPTGTVKWFNGQKGFGFIAPNDGGNDVFVHISAVERAGLSGLAEGQKVNFELKTDKMRGKTSAENLELV

Sequences:

>Translated_69_residues
MPTGTVKWFNGQKGFGFIAPNDGGNDVFVHISAVERAGLSGLAEGQKVNFELKTDKMRGKTSAENLELV
>Mature_68_residues
PTGTVKWFNGQKGFGFIAPNDGGNDVFVHISAVERAGLSGLAEGQKVNFELKTDKMRGKTSAENLELV

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=53.8461538461538, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1789979, Length=52, Percent_Identity=59.6153846153846, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1788126, Length=50, Percent_Identity=62, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787839, Length=52, Percent_Identity=57.6923076923077, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=48.4848484848485, Blast_Score=65, Evalue=8e-13,
Organism=Escherichia coli, GI1787834, Length=48, Percent_Identity=58.3333333333333, Blast_Score=63, Evalue=4e-12,
Organism=Escherichia coli, GI1787107, Length=52, Percent_Identity=51.9230769230769, Blast_Score=61, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7411; Mature: 7280

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGTVKWFNGQKGFGFIAPNDGGNDVFVHISAVERAGLSGLAEGQKVNFELKTDKMRGK
CCCCCEEEECCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCC
TSAENLELV
CCCCEEEEC
>Mature Secondary Structure 
PTGTVKWFNGQKGFGFIAPNDGGNDVFVHISAVERAGLSGLAEGQKVNFELKTDKMRGK
CCCCEEEECCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCC
TSAENLELV
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]