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The map label for this gene is amiD [H]

Identifier: 86749108

GI number: 86749108

Start: 2254367

End: 2255233

Strand: Reverse

Name: amiD [H]

Synonym: RPB_1985

Alternate gene names: 86749108

Gene position: 2255233-2254367 (Counterclockwise)

Preceding gene: 86749122

Following gene: 86749102

Centisome position: 42.3

GC content: 67.24

Gene sequence:

>867_bases
ATGTTGTCAGGTTCGTTCGCGTCTGCAGGCTCAGAGTCGAAGCGTCGACGTGCCGTCCCACTGCCCAAGGGCATCGTGGC
CATGCAGACTTTTACACCCGATTCATCCGTCGTTTCCGACGTGATCCCGTCGCCGAATTTCGGCGAGCGCAACAAGGCGC
GCCAGCCCGACATGATCGTGCTGCACTACACCGGCATGCCCGACGTCGAGGGCGCCCTGACCCGGCTGTGCAAGGCCGGC
ACCGAAGTGTCGGCGCATTACGTCGTGCTCGAGGACGGCCGGATCCTGCAATGCGTGCCGGAATCGAAGCGCGCCTGGCA
CGCCGGCGTGGCGCATTGGGCCGGCGAGGACGATATCAATTCCTGCTCGATCGGCATCGAGATCATCAATCGCGGCCATG
ATTGGGGCTATCCGGACTATCCGCTTCGCCAGATCGCCGCGGTGATCGCGCTGTGCCGCGGAATCATCATCCGCCGCAAC
GTTCAGCCGCATCGCGTGCTCGGCCATTCCGACGTGGCCCCCGCGCGCAAGAAGGACCCCGGCGAGAAATTTCCGTGGCG
GGCGCTGGCCTCCTCCGGCGTCGGGCATTGGGTCGAGCCGGCGCCGATCGATGCCGGCGCGGTGCTGCGCCATGGCGCCG
AGGGCGAACCGGTCCAGGTGCTGCAGCGCGCGCTGGCCGATTACGGCTACGGCATCGCGATCAACGGCAAGTTCGACCAC
GCGACCTTCGAGGTCGTCACCGCCTTCCAGCGCCATTTCCGGCCCGAGAAGGTCGACGGCATCGCCGACGGCTCGACGCT
GGCAACGCTGCACGCGCTGCTCGGCGGTCTGCCGGTCGAGGTCCGCACCGCCGCTGCGCTGGGCTGA

Upstream 100 bases:

>100_bases
GCCGAGTTGCGACCGACGGTCATCACACGGATAGTGGGCAGGCGCTGCGGCTGGCTGCATTCGCGCCTCCGCCGTTCTCC
TGCCGGGTTTGGATCGTCTG

Downstream 100 bases:

>100_bases
GCGACGCAGGCCCAACAACCAGCCAGATCATCGCCCTGCCGGCTCTGACGCGATCAGAGCCCGCAGGGCGATGGCGTTAT
GGCTTGTCAGCCACCGATCC

Product: negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MLSGSFASAGSESKRRRAVPLPKGIVAMQTFTPDSSVVSDVIPSPNFGERNKARQPDMIVLHYTGMPDVEGALTRLCKAG
TEVSAHYVVLEDGRILQCVPESKRAWHAGVAHWAGEDDINSCSIGIEIINRGHDWGYPDYPLRQIAAVIALCRGIIIRRN
VQPHRVLGHSDVAPARKKDPGEKFPWRALASSGVGHWVEPAPIDAGAVLRHGAEGEPVQVLQRALADYGYGIAINGKFDH
ATFEVVTAFQRHFRPEKVDGIADGSTLATLHALLGGLPVEVRTAAALG

Sequences:

>Translated_288_residues
MLSGSFASAGSESKRRRAVPLPKGIVAMQTFTPDSSVVSDVIPSPNFGERNKARQPDMIVLHYTGMPDVEGALTRLCKAG
TEVSAHYVVLEDGRILQCVPESKRAWHAGVAHWAGEDDINSCSIGIEIINRGHDWGYPDYPLRQIAAVIALCRGIIIRRN
VQPHRVLGHSDVAPARKKDPGEKFPWRALASSGVGHWVEPAPIDAGAVLRHGAEGEPVQVLQRALADYGYGIAINGKFDH
ATFEVVTAFQRHFRPEKVDGIADGSTLATLHALLGGLPVEVRTAAALG
>Mature_288_residues
MLSGSFASAGSESKRRRAVPLPKGIVAMQTFTPDSSVVSDVIPSPNFGERNKARQPDMIVLHYTGMPDVEGALTRLCKAG
TEVSAHYVVLEDGRILQCVPESKRAWHAGVAHWAGEDDINSCSIGIEIINRGHDWGYPDYPLRQIAAVIALCRGIIIRRN
VQPHRVLGHSDVAPARKKDPGEKFPWRALASSGVGHWVEPAPIDAGAVLRHGAEGEPVQVLQRALADYGYGIAINGKFDH
ATFEVVTAFQRHFRPEKVDGIADGSTLATLHALLGGLPVEVRTAAALG

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=236, Percent_Identity=38.9830508474576, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786300, Length=163, Percent_Identity=36.1963190184049, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31024; Mature: 31024

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGSFASAGSESKRRRAVPLPKGIVAMQTFTPDSSVVSDVIPSPNFGERNKARQPDMIV
CCCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCEEE
LHYTGMPDVEGALTRLCKAGTEVSAHYVVLEDGRILQCVPESKRAWHAGVAHWAGEDDIN
EEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCC
SCSIGIEIINRGHDWGYPDYPLRQIAAVIALCRGIIIRRNVQPHRVLGHSDVAPARKKDP
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCC
GEKFPWRALASSGVGHWVEPAPIDAGAVLRHGAEGEPVQVLQRALADYGYGIAINGKFDH
CCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCH
ATFEVVTAFQRHFRPEKVDGIADGSTLATLHALLGGLPVEVRTAAALG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCC
>Mature Secondary Structure
MLSGSFASAGSESKRRRAVPLPKGIVAMQTFTPDSSVVSDVIPSPNFGERNKARQPDMIV
CCCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCEEE
LHYTGMPDVEGALTRLCKAGTEVSAHYVVLEDGRILQCVPESKRAWHAGVAHWAGEDDIN
EEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCC
SCSIGIEIINRGHDWGYPDYPLRQIAAVIALCRGIIIRRNVQPHRVLGHSDVAPARKKDP
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCC
GEKFPWRALASSGVGHWVEPAPIDAGAVLRHGAEGEPVQVLQRALADYGYGIAINGKFDH
CCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCH
ATFEVVTAFQRHFRPEKVDGIADGSTLATLHALLGGLPVEVRTAAALG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]